不同结构对囊素样肽的免疫活性影响

化学合成单拷贝（BS）、30拷贝串联（KG30）和30拷贝分支结构（MAP）3种不同结构的囊素样肽，通过体外外周血淋巴细胞刺激增殖试验、杂交瘤细胞抗体分泌刺激试验、E-玫瑰花环试验和体内小鼠腹腔巨噬细胞吞噬功能、新城疫疫苗免疫应答的测定，比较了其免疫增强活性。结果表明，BS和MAP的活性显著高于KG30，BS体外试验活性优于MAP，而MAP体内试验活性优于KS。结构对囊素的活性有显著影响，以分支结构的囊素增强免疫效果最好。     

欧亚温带草原东缘生态样带研究探讨

为揭示全球变化与欧亚温带草原东缘生态系统的关系,提出沿着从中国长城至俄罗斯贝加尔湖设置一条经向样带,主要表征欧亚大陆东缘经向热量梯度,反映受大陆性气候控制下的草原地带的生态系列.沿样带开展生物地球化学过程、能量交换、植被结构动态、气候—植被、放牧—草地—牧草驯化、土地利用格局与管理、模型模拟等研究工作,对于加深气候变化和人为影响对草原生态系统的发生、发展、利用及演变规律的影响的认识,增强对气候变化的减缓和适应能力,具有非常重要的意义.     

火炬松芽苗移栽容器育苗技术

介绍了火炬松形态特征及生物学特性,并论述了其种子处理、苗床、育苗基质的准备,播种、芽苗移栽、幼苗的管理以及病虫害防治等技术措施。     

Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most widespread and destructive wheat diseases in many wheat-growing regions of the world. The winter wheat translocation line H9014-14-4-6-1 has all stage resistance. To identify stripe rust resistance genes, the segregating populations were developed from the cross between H9014-14-4-6-1 and Mingxian 169 (a wheat cultivar susceptible to all Pst races identified in China). The seedlings of the parents and F 1 plants, F2 , F3 and BC 1 generations were tested with Pst races under controlled greenhouse conditions. Two genes for resistance to stripe rust were identified, one dominant gene conferred resistance to SUN11-4, temporarily designated YrH9014 and the other recessive gene conferred resistance to CYR33. The bulked segregant analysis and simple sequence repeat (SSR) markers were used to identify polymorphic markers associated with YrH9014. Seven polymorphic SSR markers were used to genotype the F2 population inoculated with SUN11-4. A linkage map was constructed according to the genotypes of seven SSR markers and resistance gene. The molecular map spanned 24.3 cM, and the genetic distance of the two closest markers Xbarc13 and Xbarc55 to gene locus was 1.4 and 3.6 cM, respectively. Based on the position of SSR marker, the resistance gene YrH9014 was located on chromosome arm 2BS. Amplification of a set of nulli-tetrasomic Chinese Spring lines with SSR marker Xbarc13 indicated that YrH9014 was located on chromosome 2B. Based on chromosomal location, the reaction patterns and pedigree analysis, YrH9014 should be a novel resistance gene to stripe rust. This new gene and flanking markers got from this study should be useful for marker-assisted selection (MAS) in breeding programs for stripe rust.     

浅谈圆柱齿轮精度标准的发展变化

圆柱齿轮精度是影响齿轮使用寿命和性能的主要因素。圆柱齿轮精度标准也经历了几十年发展完善的过程。详细论述了圆柱齿轮精度标准的发展历程和新旧标准的变化,提醒广大企业和设计人员积极使用新标准,推广新标准。     

清代壮族边疆地区的林果生产研究

对清代壮族边疆地区的林果生产情况进行了分析,结果表明：该地区林果生产既有量的扩张,又有质的变化,市场化与近代化发展趋向明显。林果生产发展推动了壮族边疆地区乡村手工业的发展,加速了地区的开发进程。     

海南慢行旅游廊道空间体系的构建

以慢行旅游廊道空间体系为研究对象,以空间体系的单元模型构建为切入点,选取海南"大兴隆旅游区"作为其单元模型的规划实验,初步探索海南慢行旅游廊道系统空间体系的构建。可为海南建设国际旅游岛提供参考。     

肾型传染性支气管炎病毒M基因的遗传分析及其在杆状病毒表达系统中的表达

对肾型鸡传染性支气管炎病毒分离株Ck/Jiangsu/DS10/2008株(DS10株)膜蛋白(M)基因进行扩增、克隆和序列测定,并与GenBank中的其它25株参考株的M基因进行比对分析并构建进化树,研究其遗传进化关系。另将M基因克隆至杆状病毒表达系统pFastBac1载体并转染Sf9昆虫细胞进行表达,经间接免疫荧光鉴定,用琼扩试验验证表达产物的反应性,以研究M基因表达产物的生物学活性。测序结果表明DS10的M基因全长为678bp,编码225个氨基酸。遗传进化分析表明,DS10病毒与其它不同致病型的传染性支气管炎病毒共属同一大分支。间接免疫荧光检测表明,M蛋白在Sf9细胞中得到表达,琼扩试验验证表达产物具有良好的反应性,以上结果表明所表达的M蛋白具有良好的生物学活性。本研究表达的M蛋白可为其生物学活性研究及其诊断抗原、新型疫苗开发提供基础材料。     

TM600-2型离心式空气压缩机喘振故障分析及解决措施研究

离心式压缩机是生产过程中十分重要的设备,解决离心式压缩机运行的质量和效率具有重要意义。笔者通过对日常工作的总结,分析了TM600-2型离心式空气压缩机喘振故障原因,并提出了几点自己关于解决改故障的方案,实践证明很好地改善了喘振的问题。     

Mechanism of quercetin improving rat coronary artery myogenic response under high glucose

AIM: To investigate the mechanism of quercetin improving rat coronary artery myogenic response under high glucose (HG) by measuring muscle tension of coronary arterial ring and recording voltage-gated K⁺ channel (Kv) current of coronary artery smooth muscle cells by whole cell patch clamp. METHODS: The coronary rings from the normal SD rats were acutely isolated, and then divided into 6 groups: (1) control group; (2) HG group; (3) HG+low dose (3 μmol/L) of quercetin group; (4) HG+moderate dose (10 μmol/L) of quercetin group; (5) HG+high dose (30 μmol/L) of quercetin group; (6) HG+C6303 (PKC inhibitor)+high dose of quercetin group. Determinations of coronary artery response to vasoconstrictor (60 mmol/L KCl or 0.1 mmol/L U46619) or vasodilator (ACh at 10^-9~10^-5 mol/L) were performed, and the percentage of coronary ring tension was calculated using the contraction as 100% caused by 60 mmol/L KCl. The rat coronary artery smooth muscle cells were acutely isolated for recording the Kv current using whole cell patch clamp. RESULTS: Compared with control group, the contraction amplitudes to 60 mmol/L KCl or 0.1 mmol/L U46619 were significantly increased under HG incubation. Quercetin intervention concentration-dependently reduced the coronary artery contraction amplitude. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. Compared with control group, the diastolic amplitude to ACh decreased significantly in HG group, and quercetin intervention concentration-dependently increased the coronary artery diastolic amplitude. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. Compared with control group, HG incubation inhibited Kv current of coronary artery vascular smooth muscle cells significantly, and quercetin intervention attenuated the inhibitory effect of HG on Kv current intensity. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. CONCLUSION: Quercetin has a protective effect on myogenic response of coronary artery under HG and the effects is related to the increase in Kv current and the activation of PKC in vascular smooth muscle cells.