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991.
AIM: To explore the effects of curcumin analogue L6H4 on the myocardial tissue of type 2 diabetic rats and its mechanism. METHODS: Male Sprague-Dawley rats were randomly divided into normal control (NC) group, high-fat (HF) group, high-fat treatment (FT) group, diabetes mellitus (DM) group and diabetes treatment (DT) group.The rats in the latter 4 groups were fed high-fat diet for 4 weeks, then the rats in DM groups and DT groups were intraperitoneally injected with streptozotocin (STZ) to induce type 2 diabetes, while the rats in FT group and DT group were given L6H4. The blood glucose and lipid levels were detected by biochemical method, and serum adiponectin (APN) levels were detected by ELISA. The serum insulin levels were measured by radioimmunoassay and homeostasis model assessment of insulin resistance (HOMA-IR) were calculated. The morphological changes of myocardium were observed by Masson staining and electron microscopy. The protein expression of adiponectin receptor 1 (AdipoR1) and transforming growth factor β1(TGF-β1) in myocardial tissue were determined by immunohistochemistry. The protein expression of adipoR1 was also detected by Western blot for verification. RESULTS: Compared with NC group, the blood glucose, lipids, insulin, HOMA-IR and TGF-β1 were increased in HF and DM group, but they were decreased after treated with L6H4. Compared with NC group, the concentration of serum APN were decreased and the expression of AdipoR1 in the myocardium were weakened in HF group and DM group, and they increased after treated with L6H4. The myocardial fibrosis was obvious in HF group and DM group, the mitochondria in cardiomyocytes expanded, and the cristae disordered, partial disappeared. These lesions were significantly reduced after L6H4 treatment. CONCLUSION: L6H4 exerts a protective effect on the heart in type 2 diabetic rats. The increased concentration of serum APN, the enhanced expression of AdipoR1, and the expression of TGF-β1 inhibited by APN may be involved in the mechanism of protection. 相似文献
992.
HAO Xiao LI Shu-ren MENG Tian-tian GAO Qing DANG Yi XUN Li-ying YUAN Ke-xin ZHANG Qian-hui HAO Qing-qing QI Xiao-yong 《园艺学报》2016,32(3):554
AIM: To investigate the different dose of perindopril on cardiac function in the rabbits with ischemic cardiac dysfunction. METHODS: Male rabbits weighing 2.5~3.0 kg(n=30) were randomly divided into 3 groups(n=10):high dose perindopril group(HD group), low dose perindopril group(LD group) and cardiac dysfunction group(CD group). The Left anterior descending coronary artery of the rabbits was ligatured for model preparation. In HD group, the rabbits were treated with perindopril split normal saline solution(1 g/L)2 mL·kg-1·d-1. In LD group, the rabbits were treated with perindopril split normal saline solution(0.33 g/L)2 mL·kg-1·d-1. In CD group, the rabbits were treated with normal saline solution 2 mL·kg-1·d-1. Four weeks after treatment, the cardiac function was measured via echocardiography, the mRNA expression of angiotensin-converting enzyme 2(ACE2) and angiotensin type 2 receptor(AT2R) was analyzed by real-time PCR, serum angiotensin(Ang)-(1-9) and Ang-(1-7) levels were detected by ELISA. RESULTS: Compared with CD group, the cardiac function of the 2 groups treated with perindopril was significantly improved(P<0.01), and more improvement in HD group was observed than LD group(P<0.05). The serum angiotensin(Ang)-(1-9) and Ang-(1-7) level and the mRNA expression of ACE2 and AT2R in the 2 groups treated with perindopril were significantly improved(P<0.01). Compared with LD group, the mRNA expression of ACE2 and AT2R and the serum levels of Ang-(1-9) in HD group were significant improved(P<0.05), while no difference of serum Ang-(1-7) level was observed. Correlation analysis revealed that the improvement of the cardiac function was associated with serum Ang-(1-9) level, mRNA expression of ACE2 and AT2R(P<0.01), but has no significant correlation with serum Ang-(1-7) level. CONCLUSION: High dose of perindopril may improve more cardiac function in ischemic cardiac dysfunction model in rabbits. The mechanism may relate to increasing serum Ang-(1-7) level to activate AT2R. 相似文献
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为明确氟和铝对茶树[Camellia sinensis (L.) O. Kuntze]叶片叶绿素荧光特性的影响,采用盆栽沙培的方法,设置不同浓度氟和铝互作处理,采用调制叶绿素荧光仪对其荧光各参数进行分析,结果发现,茶树幼苗在未添加铝的情况下,随着氟浓度的增加,PSⅡ的实际光化学量子效率Y(Ⅱ)、最大荧光(Fm)、最大光化学效率(Fv/Fm)、光化学淬灭系数(qP和qL)降低。最小荧光Fo、非光化学淬灭系数(NPQ和qN)、PSⅡ调节性能量耗散的量子产额Y(NPQ)增加;在低铝水平下,随着氟浓度的增加,Fm、Fv/Fm、Y(Ⅱ)、NPQ和qN等值降低,而qP和qL升高,Fo、Y(NO)和Y(NPQ)值未达显著差异水平;在高铝处理下,随着氟处理浓度的增加,Fo逐渐增加,Y(Ⅱ)、Fv/Fm、qP值下降,Y(NPQ)和NPQ值在低氟时(100βmg·L-1)显著升高。可见,当受到氟胁迫时,茶树叶片光合效率会降低;添加铝可以减轻对植株造成的伤害,在低铝水平下,对提高叶片光合效率的作用更加明显。 相似文献
997.
应用亲缘关系指数分析山东省不同年代主栽花生品种的遗传多样性 总被引:6,自引:0,他引:6
应用亲缘关系指数对山东省不同年代及不同类型的主栽花生品种进行了亲缘关系和遗传多样性分析。 结果表明,约11.11% 品种组合间的亲缘系数(COP)值为0,所有供试品种COP值变异范围为0.000~0.819,平均 值为0.123,表明大多数品种在系谱上的遗传关系很小;进一步通过COP值聚类分析,在欧式距离=1.61处,可将 供试材料分为4个类群,但整体上聚类结果比较分散,说明山东省主栽花生品种遗传差异及多样性较大。 相似文献
998.
基于近等基因导入系发掘玉米抗甘蔗花叶病毒主效基因 总被引:5,自引:1,他引:5
利用玉米自交系掖478与中自01构建近等基因导入系群体(BC4F2),通过田间人工接种甘蔗花叶病毒鉴定获得抗病植株。采用38个SSR标记分析抗病株基因型,通过连锁不平衡分析,在玉米第3和6染色体上发掘3个主效抗病QTL。第3染色体上的QTL置信区间为26.1cM-phi053-5cM;第6染色体上的QTL置信区间分别为1.2cM-bnlg161和5.3cM-bnlg1538-7cM。建立了基于近等基因导入系发掘玉米抗甘蔗花叶病毒主效QTL技术,获得了一批含有抗病毒QTL的近等基因导入系,为抗病育种提供了信息和材料。 相似文献
999.
不同生育期玉米干旱-复水补偿效应的品种差异研究 总被引:4,自引:1,他引:4
以抗旱性不同的两个玉米品种郑单958和户单4号为材料,研究不同生育期干旱-复水对产量和水分利用效率的影响。结果表明,各生育期干旱-复水后,两个品种产量均低于对照处理,尤以扬花期干旱-复水最低;两个品种水分利用效率在扬花期干旱-复水最低;郑单958的产量、水分利用效率均高于户单4号,两者耗水量基本相等。分析两个品种株高、叶面积指数、干物质积累等在复水之后的变化表明,各生育期干旱-复水后,两个品种均存在一定的补偿效应,且郑单958的补偿效应明显较强。因此,郑单958在干旱-复水后补偿能力强,从而保持较高的产量和水分利用效率。 相似文献
1000.
利用微波诱变技术对产淀粉酶枯草芽孢杆菌7658进行了微波诱变效应的研究。通过对稀释液、菌悬液浓度、微波照射时间及照射距离等参数做单因素平行实验,对枯草芽孢杆菌7658的HC值以及液化作用进行比较和分析,得出各参数的最优组合,同时测定变异菌株的遗传稳定性。研究结果表明最佳诱变条件为:稀释液选用生理盐水,菌悬液浓度为10^6个/mL,照射时间为45s,微波强度为低火。 相似文献