农村电网改造规划方法的研究

农村电网的合理规划对节约优化农村有限的资金和能源、减轻农民负担、推动经济发展具有十分重要的意义。新理论和新技术的研究与应用是我国农村电网规划的大势所趋。为此,针对我国农村电网的实际情况,介绍了农网改造的缘由、意义和目的,阐述了电网规划的模型、采用的技术方法及其发展趋势。研究结果对我国现在和将来的农村电网规划与改造具有指导意义和参考价值。     

四川简阳地区砂岩层中水分利用研究

分析了四川简阳地区砂岩和土壤中的水分运动特性和砂岩中储存的水分对土壤水分补给量的多少。对当地的砂岩和土壤进行了水分特征曲线的测定试验、入渗试验和蒸发试验,并采用研究中常用模型对试验结果进行了拟合,通过对拟合结果的分析,得出了当地土壤和砂岩的水分特性,总结出了该地区表面土层在缺水时水分很容易被下部砂岩层补给,砂岩中的水分是作物利用的重要水资源的结论。这对于充分利用当地水资源,合理确定类似地质条件地区灌溉定额,解决无灌溉条件丘陵区作物缺水问题,实现农业节水灌溉有一定的参考价值。     

生活污水厌氧消化出水后处理技术研究进展

本文综述了生活污水厌氧消化系统相关后处理技术的技术特点和研究进展,着重分析了好氧处理、稳定塘处理及土地处理三种后处理技术方法,简要介绍了户用一体化装置的研究及应用状况。     

国外一种最新速度系数资料的插值求解

速度系数法是确定叶轮几何参数的一种常用方法。本文作者以数值分析方法求解了国外一种最新速度系数曲线资料的插值函数．从而为叶轮设计人员提供了一项方便利用国外先进技术．提高叶轮设计质量的有效工具。     

Progress in RNA-binding protein HuR and its roles in development of hepatocellular carcinoma

RNA-binding proteins （RBP） are molecules with a variety of biological functions discovered in recent years. Among them, HuR is an important RBP, widely expressed in various tissues of the body, and is a member of the Hu/embryonic lethal abnormal vision （ELAV） protein family. It mainly affects the expression levels of target genes in the cells by regulating the stability and/or translation efficiency of the mRNA of the genes, thus participating in the regulation of cell life activities. In recent years, more and more studies on HuR have revealed its important role in inflammation and cancer. This review summarizes the functions of HuR and probes into the regulation of HuR functions. In addition, the roles of HuR in the occurrence and development of hepatocellular carcinoma （HCC） are also discussed, which provides important information for exploring the pathogenesis, biomarkers and therapeutic targets of HCC.     

Construction of mouse ESC line stably expressing Pdx1 by Tet-On system

AIM To construct the mouse embryonic stem cell （ESC） line with stable pancreatic and duodenal homeobox 1 （Pdx1） expression by Tet-On system, which may lay a foundation for further research on the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells. METHODS The Pdx1-overexpressing lentiviral vector with green fluorescent protein marker and puromycin resistance was constructed by Tet-On system and was used to infect the mouse ESC. The cells were divided into 3 groups： blank control group （ESC group）, empty lentivirus control group （PDX1^- ESC group） and Pdx1 lentivirus transfection group （PDX1⁺ ESC group）. Flow cytometry was used to detect the transfected cells after screening by doxycycline （DOX）. The function of Tet-On system and the expression of Pdx1 gene were detected. The transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were sorted by flow cytometry, and constructed ESC line with stable expression of Pdx1 and negative control ESC line were verified. RESULTS （1） The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were 90.72% and 94.01% after screening by DOX, respectively. The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group was 97.84% and 98.13% after sorting by flow cytometry, respectively. （2） With DOX, green fluorescence was observed in PDX1^- ESC group and PDX1⁺ ESC group. The mRNA and protein expression of Pdx1 was significantly increased in PDX1⁺ ESC group （P<0.05）. Without DOX, no green fluorescence was observed in the cells of the 3 groups, and no significant difference in the mRNA and protein expression of Pdx1 was observed （P>0.05）. （3） After 3 months of cryopreservation, the cell lines still survived in resuscitation culture and were regulated by DOX. CONCLUSION Using Tet-On system, the mouse ESC line with inducible Pdx1 expression were successfully established and could be used as an effective cell model to research the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells.     

不同时期增补LED蓝光对番茄植株生长、果实品质和养分吸收的影响

以Micro-Tom番茄为试材,在营养液水培条件下,对果实发育成熟期补光(B)、果实发育期补光(B1)、果实成熟期补光(B2)下番茄植株生长、叶片光合参数、果实质量、果实品质、根系形态、矿物质离子平均吸收速率及其相互关系进行了研究,以期明确增补蓝光对番茄生长、果实品质、养分吸收的调控效果.结果 表明:B1处理能显著促进番茄的生长与果实膨大,增加果数、提高果实质量,与B处理的效果一致;B处理显著提高了番茄根系生长各项指标,B1处理显著促进主根的伸长,而B2处理对番茄根系生长无显著影响;B1处理或者B2处理显著提高了番茄果实中的有机酸含量,可溶性糖、维生素C含量、糖酸比与不补光处理差异不显著;B1与B补光处理显著提高了NO3-N、PO4-P、K+、Ca2+、Mg2+平均吸收速率;B1与B补光处理通过促进PO4-P与K+吸收,间接促进坐果,加快番茄果实发育,促进成熟与果实着色.因此,B1补光处理能达到与B补光处理相同的效果,优于B2处理.在低耗能补光策略上可以考虑采用果实发育期补光.     

微咸水灌溉模式对向日葵产量和品质的影响

为研究干旱半干旱灌区向日葵利用微咸水进行灌溉的适宜矿化度及可行性,采用淡+咸+咸畦灌模式,在向日葵苗期采用淡水灌溉,现蕾期、灌浆期采用不同矿化度微咸水2.5 g/L(KX2)、3.0 g/L(KX3)、3.5 g/L(KX4)、4.0 g/L(KX5)进行灌溉,并以全生育期淡水灌溉(KX1)为对照,研究微咸水灌溉对向日葵品质和产量的影响.结果表明:葵花籽粒中的蛋白质和油酸随着灌溉水矿化度的增加而增加,亚油酸和脂肪随着灌溉水矿化度的增加而减小,棕榈酸和硬脂酸随着灌溉水矿化度的增加呈现先增加后减小的趋势,VE随着灌溉水矿化度的增加呈现先增加后减小再增加的趋势.部分品质指标之间具有比较强的相关性,其中,蛋白质与油酸、亚油酸之间有较强的相关性,脂肪与脂肪酸各组成指标(亚麻酸除外)、VE之间有较强的相关性,脂肪酸各组成指标中除了棕榈酸与其他脂肪酸指标相关性不显著外,其他脂肪酸指标之间具有较强的相关性.并且脂肪-油酸、亚油酸-蛋白质呈正"S"变化趋势,脂肪-亚油酸呈倒"S"变化趋势;脂肪-VE、油酸-蛋白质是正相关线性关系,亚油酸-油酸是负相关线性关系,且亚油酸和油酸的相关度很高.不同处理籽粒品质指标平均综合得分排名依次为:KX5>KX4>KX2>KX3>KX1,品质指标综合得分随着灌溉水矿化度的增加而增大,不同处理向日葵平均产量依次为:KX3>KX1>KX2>KX4>KX5,产量随着灌溉水矿化度的增加而减小.因此,当灌溉水矿化度为3.5 g/L时,既不会造成向日葵大幅度地减产,又能提高其品质,是比较合理可行地微咸水灌溉浓度.     

芸薹属SSR标记在菜薹中的通用性及其应用

以4份遗传背景差异较大的菜薹种质为试材,检测133对芸薹属SSR引物在菜薹中的PCR扩增效果,以期发现适用于菜薹的SSR标记。结果表明:有50对引物在4份菜薹种质中表现出较好的通用性,其扩增产物多在100~350bp,多态性数目在1~4,共91个多态性位点。在此基础上,利用在"四九-19号菜心"和"3T6"中具有多态性的40对SSR引物,对130份以"四九-19号菜心"和"3T6"为亲本建立的F6重组自交系群体进行基因分型,构建了一个具有10个遗传群、总长度为641.39cM的菜薹遗传连锁图。研究结果可为今后菜薹遗传多样性、基因定位和分子标记辅助育种提供理论参考。     

LED白光处理对番茄贮藏品质的影响

以番茄为试材,研究了LED白光照射处理对番茄的保鲜效果的影响,为改善采后品质提供参考。结果表明:LED白光处理可有效维持番茄的感官品质,延缓其水分损失,并且不影响番茄的色泽。除此之外,还能够较好地抑制番茄可溶性固形物、维生素C含量等营养物质的降解,提高番茄的过氧化物酶(POD)和多酚氧化酶(PPO)活性,增强番茄的抗氧化能力。