Cardiomyocyte apoptosis and expression of Bcl-2, Bax protein after acute myocardial infarction and late reperfusion in dogs

AIM: To study the effect of late reperfusion on apoptotic cardiomyocytes in the risk area of acute myocardial infarctin in dogs. METHODS: The experiment was divided into three groups: sham operation group, acute myocardial infarction (AMI) group, and late reperfusion (LR) group. Apart from sham operation group, the other two groups were subjected to left anterior descending branch of coronary artery ligation. The acute myocardial infarction group was only subjected to ligation for 12 hours, late reperfusion group was subjected to ligation for 6 hours following by 6 hours of reperfusion. The cardiomyocyte apoptosis was measured by TUNEL assay. Immunohistochemistry and Western blotting analysis were used to detect the expression of Bcl-2 and Bax protein. RESULTS: The number of apoptotic cardiomyocytes in late reperfusion group was much less than acute myocardial infarction group (P<0.05), and increased significantily as compared with sham operation group (P<0.01). The expression of Bcl-2 protein was enhanced gently in late reperfusion group in contrast to acute myocardial infarction group, but no significant difference in the two groups (P>0.05) was observed, although it was much more in the two groups than that in sham operation group (P<0.01). The expression of Bax protein in late reperfusion group was much higher than that in sham operation group (P<0.01), and was lower than that in acute myocardial infarction group (P<0.05). CONCLUSION: Late reperfusion reduces cardiomyocyte apoptosis in the risk area of acute myocardial infarction. The mechanism may be that late reperfusion can decrease the expression of Bax protein.     

Adipocyte-derived relaxing factor,a new vasodilative factor

Although our knowledge of ADRF (adipocyte-derived relaxing factor) is extremely limited,the little that is known has already revealed a promising future in this newly found factor.Firstly,it is secreted by adipose tissue,which is a abundantly and extensively distributed in human body and has become a hot spot for research work in recent years.Secondly,ADRF has shown a significant vasodilative action in a considerable number of experiments conducted on arteries of various sizes,from different body parts of different species of animals,including human.In this article,we introduce the development of ADRF research,sum up its known properties,including its Ca2+,protein tyrosine kinase,and protein kinase A dependent releasing,K+ channel mediated functioning,and interfered effect in different pathological models,and propose problems surrounding this factor and directions for future research work.     

松叶蜂病原微生物的研究进展

本文对我国松叶蜂几个危害区幼虫期或茧期的病原进行了调查，统计了松叶蜂类害虫的为害和病原的寄生情况。详细描述了国内外已分离的主要松叶蜂病毒各自超微结构的特征、所属类型、及其一系列研究近况。总结了近年来国内外利用松叶蜂病原微生物防治该类害虫取得的成果、目前所存在的问题。还就我国利用松叶蜂病原防治该虫进行了展望。     

基于校企合作的CDIO工程教育模式改革实践

针对CDIO工程教育体系滞后、校企合作深度不够等问题,探索基于校企合作的CDIO工程教育模式改革实践.分析现有校企合作人才培养存在的问题,提出实施CDIO工程教育,校企共建新体系;整合资源,营造工程实践教学环境;结合真实企业案例,奠定CDIO教育新基础;完善师资队伍建设,改革师资培养策略等改革,以提升学生综合职业能力和就业率.     

Cloning of Buffalo AQP9 Gene and Analysing its Expression in the Buffalo Tissues

Cloning buffalo AQP9 gene and analyzing its expression in buffalo tissues.A pair of primers was designed according to the released bovine AQP9 sequences in GenBank,which was used to clone buffalo AQP9 gene.The AQP9 gene was amplified by RT-PCR,whose nucleotide sequence and protein structure were analyzed by bioinformatics methods.The expression of AQP9 in buffalo tissues was assayed by Real-time quantitative PCR.The expression of AQP9 gene in buffalo ovary and testis tissue was detected by immunohistochemical staining method.The results showed that the cloned ORF length of buffalo AQP9 gene was 888 bp,which coded 295 amino acids.The results of multiple sequence comparison showed that the nucleotide sequence of buffalo AQP9 shared 99%,90%,97% and 88% homologeous compared with that of Bos taurus,Sus scrofa,Ovis ariessis and Homo sapiens,respectively,while shared 99%,86%,97%,83% homologeous for amino acids,respectively.Phylogenetic tree analysis indicated that AQP9 gene was highly conservative in the evolutionary process.Real-time quantitative PCR results showed that AQP9 gene expressed in buffalo liver,lung,brain,skin,testis and ovary tissues with different levels,had the most abundant expression in liver,followed by in skin and testis,less observed in lung and ovary.The results of immunohistochemical staining showed that the expression of AQP9 protein varied with the development of buffalo ovarian tissue,and gradually enhanced with follicle development.In testicular tissue,AQP9 protein expressed in spermatocyte and leydig cells of developmental stage testis.These results indicated that we had successfully cloned buffalo AQP9 gene sequences.The expression and its function of AQP9 in buffalo ovaries and testes might play an important role in follicle development and spermatogenesis.     

聚合物发光二极管的研究进展

对共轭聚合物中进行电运输及电致发光器件－聚合物发光二极管的研究进展进行了综述。     

透光抚育对红松阔叶林群落结构与生产力的影响

采用样带网格调查方法和群落重要值分析方法，研究透光抚育（全透光、半透光、未透光）对红松阔叶混交林群落（人天混群落）的树种组成结构、径级分布、蓄积量和红松蓄积生产力的影响效果。结果表明：沿由小到大的透光强度，人天混群落中红松重要值依次增大（0．499～1．000），透光有利于人天混群落中红松优势地位增强；红松大径木比例依次增加（0．00％～54．0％），小径木比例依次减少（74．9％～2．7％），透光有利于人天混群落中红松大径木比例提高；群落蓄积量依次增加（60．2475～230．4051m^3／hm^2），红松蓄积生产力依次增加（1．2692～5．3603m^3／hm^2·a），透光有利于人天混群落蓄积量及红松蓄积生产力的提高。     

弓形虫半巢式PCR检测方法的建立

根据GenBank上公布的弓形虫RH株的P30基因(X14080)设计了3条特异性引物P1、P2和P3,建立了半巢式PCR检测体系,并将P1和P3的扩增产物克隆到pUCm-T载体上进行测序.结果表明,该体系能够扩增出约250 bp的片段,P1和P3能扩增出约500 bp的片段,克隆到pUCm-T载体上的504 bp片段与P30基因(X14080)的序列同源性为99.8%.该PCR检测体系特异性强,在健康猪血液、猪链球菌、多杀性巴氏杆菌、胸膜肺炎放线杆菌和猪圆环病毒DNA上未扩增出条带;敏感性高,最低检测为18 fg.该检测体系的成功构建为猪弓形虫的检测和流行病学调查提供了有力的技术支持.     

中国商业银行财务竞争力的评价研究

将商业银行竞争力的评价指标明确界定为财务竞争力指标扣非财务竞争力指标，考察2006年我国商业银行的财务竞争力可知，在体制转轨扣入世后金融领域逐步开放的情况下，我国商业银行竞争力受到现行体制的影响，银行业的改革扣发展具有一定的路径依赖效应。要提升商业银行的竞争力。政府应当正确发挥其监管扣保护作用。     

云南省屏边县洋麻引种试验

中红麻11号和H305两个洋麻品种生物学特性相对较好,亩产量高,抗性强,可在屏边地区进一步进行多点验和推广种植.