Four bacterial blight (BB) resistance genes,
Xa7,
Xa21,
Xa22 and
Xa23, were introgressed into an elite hybrid rice restorer line Huahui 1035, by marker-assisted selection (MAS). Ten promising
BB resistant lines identified by MAS approach in Huahui 1035 restorer background and their respective F
1 hybrids with a cytoplasmic male sterile line i.e. Jinke 1A, three BB resistant gene donors and their recurrent parent were
evaluated for BB resistance by inoculating them with eleven representative races of
Xanthomonas
oryzae pv
. oryzae (
Xoo) in China. Further, the ten marker assisted selection of BB (MAS-BB) resistant restorer lines and their F
1 hybrids were also characterized for agro-morphological traits and grain yield. Results revealed that restorer lines with
Xa23 introgression i.e. HBQ809 and HBQ810 were found to be resistant to all eleven Chinese representative
Xoo races, showing broad spectrum resistance to BB. However, the lines possessing
Xa7 or
Xa7+
Xa21 were resistant to ten out of eleven
Xoo races. While restorer lines with
Xa21 were resistant to nine out of eleven
Xoo races. Interestingly, the F
1 hybrids with
Xa23,
Xa7 or
Xa7+
Xa21 were resistant to two severe epidemic
Xoo races of China. Restorer lines i.e. HBQ807 and HBQ808 possessing
Xa22 were only resistant to six or five out of inoculated eleven
Xoo races. Lesion length comparisons between restorer lines and their F
1 hybrids for the above four BB resistance genes showed them to be dominant in heterozygote genotype. Three promising high
yielding F
1 hybrids with BB resistance were identified for immediate exploitation for hybrid rice production in China.
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