Effects of external counterpulsation on renin-angiotensin system and hemodynamics in dogs with myocardial ischemia

AIM:To examine the effect of external counterpulsation(ECP) on renin-angiotensin system(RAS) and hemodynamics in dogs with myocardial ischemia and their relationship. METHODS:Acute myocardial ischemia was induced by occluding the left anterior descending of the dogs. The local renin activity, angiotensin Ⅱ(AngⅡ) level, angiotension-converting enzyme(ACE) activity were tested by biochemical methods and hemodynamics was recorded by a 8-channel physiological recorder.RESULTS:Ischemia could actiivate renin,ACE and AngⅡ in cardiovasculature and ECP reduced them except for renin in ischemic myocardium. Ischemia also could activate RAS in lung and kidney, which play an important role on circulating RAS, and ECP reduced them. Furthermore, ECP could improve hemodynamics and there existed a close relationship between local AngⅡlevel and hemodynamics. CONCLUSION:ECP can reduce local RAS and improve hemodynamics in dogs with myocardial ischemia, which might be one of mechanisms underlying the protective effect of ECP on ischemic myocardium.     

12-(取代苯氧异丁酰氧亚氨基)-1,15-十五内酯的合成及除草活性

通过取代苯氧异丁酸与12-(羟基亚氨基)十五内酯的反应,合成了取代苯氧异丁酰氧亚氨基十五内酯,并测定了其对苋菜的除草活性。所有化合物均经过1H NMR、13C NMR和元素分析确证。初步的生物活性测定结果表明,目标化合物 Ⅲa~Ⅲd 的EC50值分别为34.570、46.492、55.385、50.114 mg/L,其活性比对照药剂2,4-D(117.325 mg/L)高,而比苯磺隆(22.381 mg/L)低。     

灰树花不同配方栽培研究

对灰树花七种栽培配方进行了研究，其结果表明：木屑和棉籽壳作为栽培的主要原料，若等比例混合。生物学效率最高；麦麸与玉米粉搭配使用，可以提高灰树花的产量及生物学效率；平菇废料是栽培灰树花较好的原料，并具有很好的经济效益、社会效益和生态效益；并筛选出灰树花高产的三个配方。     

国产克洛素隆尿药排泄数据分析

应用Kromasil-C18色谱柱(250 mm×4.6 mm,5 μm),WatersTM480型可调波长紫外检测器,0.01M磷酸钾(pH=7):乙腈(3:1)为流动相,检测波长265 nm,含量测定采用标准曲线法,建立了RP-HPLC法检测绵羊尿中克洛素隆含量的方法.方法有效性评价结果表明,尿药含量在0.01～5.0μg/ml及5.0～30.0μg/ml范围呈良好线形关系(r=0.9993、0.9995),方法平均回收率99.32%,日内、日间变异系数分别为3.91%、6.28%.尿药最低检测限0.005μg/ml.试验绵羊以7 mg/kg单剂量经静脉、肌肉及口服三种途径给药后,尿中药物浓度分析表明,克洛素隆经体内处理后主要经肾脏排泄,给药96 h内,静注经尿排泄原药为81.76%,肌注为64.03%,口服为48.50%.     

山豆根多糖体外清除自由基作用的研究

采用邻二氮菲-Fe2 氧化法测定了山豆根多糖(SSP1)清除羟自由基(.OH)的作用,应用邻苯三酚自氧化反应体系为产生超氧阴离子自由基(O2.)模型观察了SSP1对超氧阴离子的清除作用,选择辣根过氧化物酶-酚红法测定了山豆根多糖对H2O2的清除作用.结果表明,山豆根多糖(50～400 mg/l)具有清除羟自由基的作用,且呈明显的量效关系;对邻苯三酚自氧化具有抑制作用,对调理酵母多糖诱导的小鼠脾脏淋巴细胞释放H2O2具有抑制作用.该结果提示山豆根多糖具有抗氧化作用.     

富铁酵母对家兔红细胞免疫功能的影响

为探讨富铁酵母对家兔红细胞免疫功能的影响,将32只家免随机分成富铁酵母高、低剂量组,FeSO4组及空白对照组,分别灌服富铁酵母(Fe元素8 mg/kg、4 mg/kg)、FeSO4(Fe元素4 mg/kg)及空白生理盐水;连续5 d后,检测红细胞C3g受体花环率和复合物(IC)花环率.结果:富铁酵母较FeSO4及空白组能显著提高红细胞C3h受体花环和红细胞复合物(IC)花环的百分率,但高、低剂量间无显著性差异.结论:富铁酵母能显著提高红细胞免疫功能.     

加强自身卫生防护防止传播动物疫病

随着我国畜牧养殖业的不断发展，动物防疫检疫人员在依法严格把关、确保动物及其产品检疫质量、保护人民身体健康、促进养殖业健康发展等方面，做出了积极努力和贡献。但动物防疫检疫人员作为与各种动物及其产品的密切接触者，其自身对消毒防护措施的重视程度，与动物疫病的发生和流行有着一定的关系。     

玉米螟赤眼蜂对亚洲玉米螟种群的控制作用

为了解决深圳特区甜玉米产区日趋严重的农药污染问题,笔者在深圳龙岗生态示范农场甜玉米地研究了玉米螟赤眼蜂Trichogramma ostriniae的应用技术,分析了玉米螟赤眼蜂释放量、释放次数对亚洲玉米螟Ostrinia furnacalis Guen6e控制作用的关系,提出了最件释放方法.     

南瓜新品种龙园栗香的选育

龙园栗香南瓜是从日本和韩国引进品种中 ,经分离选育的自交系 93- 12 - 8和 93- 9- 7配制的早熟南瓜一代杂种 ,果实扁圆形 ,果皮墨绿色 ,覆白绿色点条斑纹 ,平均单瓜质量 2 .0kg ,果肉桔黄色 ,可食率达 80% ,肉质香面适口、品质佳。从播种至采收 80d(天 )左右 ,较抗白粉病和病毒病 ,平均产量逾 5 0 0 0 0kg·hm-2 。     

Expression of CD44s in lung cancer and its significance

AIM: To investigate expression of CD44s in lung cancer and it's clinical significance. METHODS: A total of 117 primary lung cancer from patients were examined for CD44s expression by immunohistochemical staining. RESULTS: CD44s mostly expressed in non-small cell lung cancer (NSCLC) but not in small ecll lung cancer (SCLC), and squamous cell carcinoma(SCC) showed much stronger expression of CD44s than adenocarcinoma(ADC)(P＜0.05). In comparison of the lung cancer with/ without lymph node metastasis, the latter showed stronger expression of CD44s(P＜0.01). According to TNM, there was a distinct statistic difference between early stage and advanced stage(P＜0.05). CONCLUSION: CD44s might be a better indicant in histological classification of lung cancer, lymph node metastasis, clinical stage and prognosis.