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151.
152.
吉林农业科技学院校园网络用户数量庞大,但是校园网络带宽资源有限,人均带宽不足,导致上传资源、下载资料速度缓慢。本文详细阐述了吉林农业科技学院"阳光下"服务交流平台的网站功能规划、前台设计、数据库的实现和服务器搭建、系统门户的架构和系统测试与维护。 相似文献
153.
Weiping HUANG Masashi NAGANO Sung-Sik KANG Yojiro YANAGAWA Yoshiyuki TAKAHASHI 《The Journal of reproduction and development》2014,60(1):9-13
The objective of this study was to clarify the effects of prematurational culture
(pre-IVM) supplemented with 3-isobutyl-1-methylxanthine (IBMX) on nuclear and cytoplasmic
maturation of in vitro-grown bovine oocytes. In experiment 1, oocytes (95
μm in diameter) derived from early antral follicles (0.5–1 mm in diameter) were cultured
for 12 days for in vitro growth (IVG). IVG oocytes with a normal
appearance were subjected to examinations of diameter and chromatin structure in the
germinal vesicle (GV) before IVM. In addition, percentages of metaphase II (M II) were
examined after IVM. Regardless of pre-IVM, the mean diameters of IVG oocytes were about
115 μm. The proportions of GV3 (50.0%) and M II stages (80.1%) of IVG oocytes with pre-IVM
were higher than those without pre-IVM (28.0 and 49.4%, respectively). In experiment 2,
the fertilizability and developmental competence of IVG oocytes were examined. Regardless
of pre-IVM, the normal fertilization rates of IVG oocytes were similar (around 70%) but
were lower than that of in vivo-grown oocytes (88.0%). Cleavage and
blastocyst rates of IVG oocytes with pre-IVM (63.0 and 26.1%, respectively) were higher
than those without pre-IVM (45.8 and 12.7%, respectively). The blastocyst rate based on
cleaved IVG oocytes with pre-IVM (41.7%) was similar to that of in
vivo-grown oocytes (48.7%), although the cleavage rate of IVG oocytes with
pre-IVM was lower than that of in vivo-grown oocytes. In conclusion,
pre-IVM with IBMX improved the maturational and developmental competences of IVG oocytes,
probably due to promotion of their chromatin transition and synchronization of meiotic
progression. 相似文献
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155.
皱纹盘鲍是我国北方海珍品的主要养殖种类,其特殊的生长习性、营养及药用价值说明它的消化能力强,体内具有活性强的酶。近年来,鲍内脏酶的研究越来越为人们所重视,特别是鲍内脏中的褐藻酸酶、纤维素酶、琼脂酶的研究和应用。本文主要研讨鲍内脏酶的组成、制备法及其应用前景。 相似文献
156.
[目的]研究铬胁迫下薏米对含铬污水的净化作用及铬胁迫对薏米生理生化的影响,为人工湿地净化含铬污水提供理论支持.[方法]开展桶栽模拟人工湿地试验,以薏米为材料,定期浇灌不同浓度的Cr(Ⅵ)生活污水(0、5、20、40、60mg/LK2Cr2O7),定期测定进出水的化学需氧量(COD)和总磷(TP),并检测薏米叶片过氧化物酶(POD)、超氧化物歧化酶(SOD)、丙二醛(MDA)等指标.[结果]薏米对含铬污水中COD的去除率随Cr(Ⅵ)浓度增大而降低,5mg/L的Cr(Ⅵ)处理在对总磷的去除率在90%以上,并随Cr(Ⅵ)浓度的升高而下降;薏米叶中的MDA含量随Cr(Ⅵ)浓度和处理时间的增加而呈增加趋势;叶片SOD、POD活性均随处理时间的延长而呈先上升后下降的趋势.[结论]薏米能耐受一定浓度的Cr(Ⅵ)胁迫,且对水质净化有较好的作用. 相似文献
157.
158.
本试验旨在研究不同混合比例对甘薯蔓、酒糟及稻草混合青贮品质的影响。试验设5个组:对照组(鲜甘薯蔓单一青贮)、凋萎组(经2 d自然晾晒处理的甘薯蔓)、40%组(40%鲜甘薯蔓+40%酒糟+20%稻草混贮)、55%组(55%鲜甘薯蔓+25%酒糟+20%稻草混贮)、70%组(70%鲜甘薯蔓+10%酒糟+20%稻草混贮),每个组10个重复,压实密封后室温贮藏60 d,取样分析青贮品质。结果表明:1)甘薯蔓与酒糟和稻草混合以及凋萎处理后青贮均改善了其感官品质;2)与对照组相比,凋萎组干物质、粗蛋白质、乳酸含量极显著增加(P<0.01),氨态氮/总氮、丙酸、丁酸含量及p H极显著降低(P<0.01);3)与对照组和凋萎组相比,3个混贮组干物质、中性洗涤纤维、酸性洗涤纤维、乳酸含量都极显著增加(P<0.01),氨态氮/总氮、粗蛋白质、粗灰分含量及p H极显著降低(P<0.01);4)随着混贮组中甘薯蔓比例的升高,干物质含量极显著降低(P<0.01),粗蛋白质含量显著降低(P<0.05),氨态氮/总氮、丙酸含量极显著增加(P<0.01);5)与青贮前各组原料相比,对照组和凋萎组干物质、粗蛋白质损失较多,40%组损失最少。综上所述,鲜甘薯蔓、酒糟及稻草混合比例为40∶40∶20青贮品质最优。 相似文献
159.
KANG Jing-jing ZHAO De-ming TENG Ke-dao JIAO Xi-lan WANG Ping-li SUN Zhe NI Pei-pei WANG Zhi-feng ZHANG Rui YANG Yu-rong LIANG Hong-de 《农业科学学报》2014,13(4):858-869
Ulcerative colitis (UC) is a lifelong illness with profound emotional and social impacts, and could cause serious damage to large intestine, especially in colon. However, the pathogenesis of UC remained unclear. The present study attempts to find out the role of matrix metalloproteinases-7 (MMP-7) and lysozyme in the pathogenesis of UC through a mice model induced by dextran sulfate sodium (DSS). The UC model was evaluated both by disease activity index (DAI) and the intestinal histopathology. The results show that there is a high correlation between the DAI score and the pathological changes of colon. Interleukin-6 (IL-6) serum levels and large intestinal fluids levels in UC mice are always higher than that of the control groups, which might be associated with the degree of the inflammation damage in the colon. The change tendency of the MMP-7 mRNA and protein expressions are both up-regulated firstly and then down-regulated from 1 to 5 d in the colon, but only the MMP-7 protein is up-regulated at 7 d again. The up-regulated MMP-7 levels in the early stage of UC may play a protective role through the activated defensins, while the down-regulated levels in the mid-later stage of UC may be connected with the severe lesions in the colon. However, the up-regulated MMP-7 levels in the later stage of UC in the colon may also contribute to the tissue repair or be served as a marker to CRC (colorectal cancer). The distribution of lysozyme protein indicates that there may be Paneth-like cells in the colon. Both the changes of MMP-7 and lysozyme in the small intestine may play a protective role for the safe environment of the whole gut, especially to the colon of UC. 相似文献
160.