条件性敲除D1133L基因的重组非洲猪瘟病毒的构建及增殖特性

前期研究初步表明非洲猪瘟病毒(ASFV)编码的D1133L基因对ASFV复制至关重要,本研究拟进一步探究D1133L在ASFV复制中的作用。利用同源重组技术结合大肠杆菌lac阻遏操作系统实现条件性敲除D1133L基因,以pUC118为骨架重组转移载体ASFVΔi130,将重组转移载体转染骨髓源巨噬细胞(BMDMs),以ASFV CN/GS/2018为亲本毒株感染BMDM,在β-D-硫代半乳糖苷(IPTG)存在的条件下,经绿色荧光和PCR鉴定,获得条件性敲除D1133L重组毒株vD1133Li。利用荧光显微镜观察该重组毒株与亲本毒株在猪肺泡巨噬细胞(PAMs)中的复制差异,利用qPCR技术比较重组病毒与亲本毒株的复制差异,分析vD1133Li在无IPTG情况下回补D1133L蛋白后与在IPTG诱导情况下的复制差异。结果显示：本研究成功构建了条件性敲除D1133L的ASFV重组病毒vD1133Li,重组毒株不表达D1133L,在IPTG诱导下复制能力显著低于亲本毒株;在稳定表达D1133L的MA-104细胞系中,vD1133Li复制能力恢复。综上所述,D1133L基因对于ASFV复制至关重...     

谷物及种子的力学--流变学特性的研究进展

近年来，国内外广泛开展了固体农业物料力学流变学特性的研究。为此，概述了国内外谷物及种子的力学流变学特性方而的研究及进展情况，同时介绍了这一特性在农业工程中的应用。     

Dynamic Modeling and Simulation of 440 t/h Steam-cooled Cyclone Separator for CFB Boiler

A dynamic mathematical model of the steam-cooled cyclone separator in a 440 t/h CFB boiler has been established.Based on the mathematical model,simulation model of the cyclone separator is created with the advanced continuous simulation language(ACSL).The validity of this model has been verified by the simulation experiments and analysis.This model can provide a theoretical guidance for operation and optimal design of the cyclone separator.     

Exploration of Implementation Strategieson Housing Building Technology

Due to the sharp contradiction between rapidly developing housing construction in small-size town and quite low technological level, in this paper, the significance of setting up integrally technological system is emphasized and the suggestion that the technology should be integrated with the sustainable development, regional context and industrialization is put forward.     

基于小波分析的玉米籽粒图像正形研究

近年来,通过对玉米籽粒的尺寸、外形、色泽等方面的研究,已获得了一些必要的单籽粒的视觉信息。但是,对于粮食作物而言,只有实现了作物籽粒的大规模、快速识别,才有可能在实际生产中得到广泛应用。为此,采用图像标记法,对含有众多的、散放的玉米籽粒图像进行处理,对标记图像中的像素进行逻辑判断,采用多尺度小波分析算法,实现籽粒分割、定位及正形。     

番茄果实力学特性及与成熟度关系的试验研究

采用完整番茄果实为试样,根据弹性力学赫芝理论及线形粘弹性理论,结合图像处理的方法,从平板压缩试验及应力松弛试验研究中,提取力学—流变学性质参数,并为果实的成熟度判别作应用探讨,为番茄的生产、运输,加工及分级等提供必要的方法与基础数据资料。     

园林灌溉对再生水回用的技术要求

结合目前再生水回用的必要性和紧迫性问题,就污水处理相关技术与工艺、再生水灌溉的技术要求与标准以及再生水园林灌溉的管理和监测等方面,对再生水应用于园林灌溉进行了综合阐述,并提出了相应的技术要求.     

粮食生产的机械化问题

粮食牛产机械化是生产力发展的一个重要方而，也是一种必然趋势。机械化生产手段可以提高粮食产量，确保粮食生产持续发展，提高粮食生产效率和经济效益。粮食生产机械化不仅是农业技术变迁的一种自然发展过程，而且与制度变迁密切相关、互相促进。在粮食生产机械化中，有很多现实问题需要解决。     

Effects of arrangement of surge-rootirrigation emitters on growth,yield andwater use efficiency of apple trees

Six-year old apple trees were selected for field experiment. The objective of this study was to obtain the reasonable arrangement of surge-root irrigation emitters in apple orchards. There were three factors: the buried depth H( 25,40,55 cm),the horizontal distance L( 30,40,60 cm) between the emitters and the trunk of the experimental tree,and the number of the irrigation emitters N( 1,2,4). The effect of the arrangement of surge-root irrigation emitters on the growth,yield and irrigation water use efficiency( IWUE) of apple trees were studied in Northern Shaanxi where the irrigation quota takes 60%-75% of the field water capacity. The results showed that the arrangement of emitters for surge-root irrigation had a significant effect on apple tree yield and IWUE,especially,the yield and IWUE reached 28 388. 17 kg/hm2 and 16. 83 kg/m3 in treatment T3,respectively. At the same L and N levels( T1,T2,and T3),the yield and IWUE in treatment T3 were the highest,and the yields in treatments T1 and T2 were decreased by 26.22% and 31.48%,while IWUE is reduced by14.02% and 18.12% compared with T3,respectively. At the same H and N levels( T3,T4,and T5),the yield and IWUE of apple trees were decreased with increasing L level. Especially,when L was 30 cm( T3),the yield and IWUE were the highest. The same L and H levels( T3,T6,and T7) could promote the growth of apple trees when N was 2( T3). Compared with treatment T3,it was found that the increment of new shoots was decreased by 8.07%-18.71%,and the fruit diameter was decreased by 5.41%-9.11%. Therefore,two emitters should be arranged symmetrically on both sides of an apple tree,each was buried at a 40 cm depth and 30 cm away from the trunk of the tree to effectively improve the yield and IWUE of the apple tree in mountainous areas in Northern Shaanxi.     

实用型CAPP开发工具系统的实现

介绍了CAPP开发工具系统的工作原理，分析了开发中遇到的问题和关键技术，并提出了解决办法。