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101.
Niu Chen Li Yun 《中国林学(英文版)》2006,8(1):32-34
Conventional methods of DNA recovery from agarose gel generally require expensive equipment, extended elution times, or considerable handling of the sample after elution. We developed a simple protocol for a quick and effective recovery of DNA from agarose gels with good yield and quality. Using a Sephadex resin filled spin column, DNA fragments of 500 bp to 6 kb in an agarose gel slice were easily recovered by a 2 min centrifugation. The recovery efficiencies were over 40%-50% and the eluted DNA can be used directly for downstream application, such as polymerase chain reactions (PCR) and restriction enzyme digestion. This method could also be used to recover large DNA fragment (48 kb) without degradation. The use of Sephadex helps to remove small molecular impurities from agarose and it also reduces the chance of clogging the column filter caused by direct contact with agarose. 相似文献
102.
以I-69杨嫩枝、半硬枝和一年生硬枝为试验材料,研究了乙烯在茎插穗不定根形成中的作用。生长素类物质处理可诱导乙烯释放,在扦插生根的整个过程中,乙烯释放量均比对照高,同时促进不定根形成。而用GA_3、ABA和KT处理,乙烯释放量均与对照相同,它们不促进,甚至抑制或强烈抑制不定根形成。用浓度为25μg·ml~(-1)-1000μg·ml~(-1)的乙烯气体处理,均能促进I-69杨硬枝插穗不定根形成和芽的萌发。乙烯利处理,低浓度(小于25μg·ml~(-1))促进,随浓度提高从无促进到抑制。高浓度乙烯利、IBA和NAA处理插穗其形态变化相似。试验结果认为乙烯对I-69杨插穗不定根形成的作用与生长素相类似,低浓度促进,高浓度抑制。 相似文献
103.
104.
YuXinxiao NiuJianzhi XuJunliang 《中国林学(英文版)》2004,6(3):28-35
The paper provides a systematical analysis of ecological restoration effects of natural secondary forest of closurearea in Chao Guanxi Gou, Miyun County, Beijing. The results indicate that through more than twenty years of hillclosing afforestation since 1983, canopy closure has improved almost by 0.2; forest cover rate has raised from 7.2% to 93.8%; biodiversity, tree biomass and vegetation community have increased to a great extent. Compared with the average canopy closure before hillclosing afforestation in this area, it has improved to over 0.4 in average and increased by 0.1-0.2. The forest coverage degree has reached more than 90%. Consequently, the forest plays more important roles in intercepting precipitation, improving water storage capacity of soil, decreasing the surface runoff, and preventing soil and water loss. 相似文献
105.
本文针对正态总体的情形,讨论了如何利用试差法确定样本容量,即利用 n=1+s~2/(Δ (?)~2)·t~2_(n-1)(a) 式反复迭代,直到求出的n值差异很小为止,这时的n值即为所求的样本容量。文中并给出了试差法在林业中的应用实例。 相似文献
106.
107.
森林生态系统碳循环研究方法概述 总被引:2,自引:0,他引:2
介绍了森林生态系统碳循环研究方法,对当前森林生态系统碳循环研究的几种主要方法模型进行了概述,主要有Miami模型和Thornthwaite模型、Chikugo模型和生物量转换因子连续法等。 相似文献
108.
不同光质对长鞭红景天悬浮细胞生长及苯丙氨酸解氨酶活性的影响 总被引:4,自引:0,他引:4
对不同光质条件下长鞭红景天悬浮细胞生物量及苯丙氨酸解氨酶(PAL)的活性水平进行检测,探讨长鞭红景天次生代谢和环境调节之间的相关性.结果表明:1)长鞭红景天悬浮培养细胞生物量变化为S型曲线,6~9 d进入直线生长期,PAL活性也表现出一定的规律性,在细胞指数生长期急剧上升,至细胞干质量最大时,活性最大,生长停止期时急速下降至初始水平;2)不同光质在较长时间辐射处理下,长鞭红景天悬浮细胞生物量表现出一定的差异,PAL活性也随之不同,其中长时间的红光处理有利于酶活性的提高,但生物量显著低于白光处理. 相似文献
109.
110.
Yu Niu Yongwei Zhao Jintian He Yang Yun Mingming Shen Zhending Gan Lili Zhang Tian Wang 《动物营养(英文)》2021,7(3):667
The aim of present study was to evaluate whether diets supplemented with dihydroartemisinin (DHA) could alleviate intestinal inflammatory injury in weaned piglets with intrauterine growth retardation (IUGR). Twelve normal birth weight (NBW) piglets and 12 piglets with IUGR were fed a basal diet (NBW-CON and IUCR-CON groups), and another 12 piglets with IUGR were fed the basal diet supplemented with DHA at 80 mg/kg (IUGR-DHA group) from 21 to 49 d of age. At 49 d of age, 8 piglets with similar body weight in each group were sacrificed. The jejunal and ileal samples were collected for further analysis. The results showed that IUGR impaired intestinal morphology, increased intestinal inflammatory response, raised enterocyte apoptosis and reduced enterocyte proliferation and activated transmembrane toll-like receptor 4 (TLR4)/nucleotide-binding and oligomerization domain (NOD)/nuclear factor-κB (NF-κB) signaling pathway. Dihydroartemisinin inclusion ameliorated intestinal morphology, indicated by increased villus height, villus height-to-crypt depth ratio, villus surface area and decreased villus width of piglets with IUGR (P < 0.05). Compared with NBW piglets, IUGR piglets supplemented with DHA exhibited higher apoptosis index and caspase-3 expression, and lower proliferation index and proliferating cell nuclear antigen expression in the intestine (P < 0.05). Dihydroartemisinin supplementation attenuated the intestinal inflammation of piglets with IUGR, indicated by increased concentrations of intestinal inflammatory cytokines and lipopolysaccharides (P < 0.05). In addition, DHA supplementation down-regulated the related mRNA expressions of TLR4/NOD/NF-κB signaling pathway and upregulated mRNA expressions of negative regulators of TLR4 and NOD signaling pathway in the intestine of piglets with IUGR (P < 0.05). Piglets in the IUGR-DHA group showed lower protein expressions of TLR4, phosphorylated NF-κB (pNF-κB) inhibitor α, nuclear pNF-κB, and higher protein expression of cytoplasmic pNF-κB in the intestine than those in the IUGR-CON group (P < 0.05). In conclusion, DHA supplementation could improve intestinal morphology, regulate enterocyte proliferation and apoptosis, and alleviate intestinal inflammation through TLR4/NOD/NF-κB signaling pathway in weaned piglets with IUGR. 相似文献