不同光质对长鞭红景天悬浮细胞生长及苯丙氨酸解氨酶活性的影响

对不同光质条件下长鞭红景天悬浮细胞生物量及苯丙氨酸解氨酶(PAL)的活性水平进行检测,探讨长鞭红景天次生代谢和环境调节之间的相关性.结果表明:1)长鞭红景天悬浮培养细胞生物量变化为S型曲线,6～9 d进入直线生长期,PAL活性也表现出一定的规律性,在细胞指数生长期急剧上升,至细胞干质量最大时,活性最大,生长停止期时急速下降至初始水平;2)不同光质在较长时间辐射处理下,长鞭红景天悬浮细胞生物量表现出一定的差异,PAL活性也随之不同,其中长时间的红光处理有利于酶活性的提高,但生物量显著低于白光处理.

Effects of Different Light Treatments on Growth and PAL Activity of the Suspension-Cultured Cells of Rhodiola fastigiata

The biomasses and the phenylalanine ammonia lyase (PAL) activities were tested in suspension-cultured cells of Rhodiola fastigiata under different light treatments, and relationship between development of the secondary metabolism of R. fastigiata and the environment factors were investigated. The results showed that: 1) Biomasses of the suspension-cultured cells changed in a typical S curve. The biomasses reached a linear growth phase for 6 to 9 d, and the PAL activity also showed the similar change. The PAL activity increased rapidly at the exponential growth phase of the suspension-cultured cells, and reached the maximum at the time of maximum dry-mass and decreased to initial level when growth of the cells stopped; 2) Biomasses of the suspension-cultured cells of R. fastigiata showed difference under different light treatments, and the activity of PAL also changed at the same timing. Among these light treatments, the continuous red light treatment was of advantage to the increase of the PAL activity. However, the biomass under red light was obviously lower than that under white light.