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排序方式: 共有267条查询结果,搜索用时 140 毫秒
261.
Banks JA Nishiyama T Hasebe M Bowman JL Gribskov M dePamphilis C Albert VA Aono N Aoyama T Ambrose BA Ashton NW Axtell MJ Barker E Barker MS Bennetzen JL Bonawitz ND Chapple C Cheng C Correa LG Dacre M DeBarry J Dreyer I Elias M Engstrom EM Estelle M Feng L Finet C Floyd SK Frommer WB Fujita T Gramzow L Gutensohn M Harholt J Hattori M Heyl A Hirai T Hiwatashi Y Ishikawa M Iwata M Karol KG Koehler B Kolukisaoglu U Kubo M Kurata T Lalonde S Li K Li Y Litt A Lyons E Manning G Maruyama T Michael TP 《Science (New York, N.Y.)》2011,332(6032):960-963
262.
Marek Wełnicki Jacek L. Skrzeczkowski Włodzimierz Zagorski Stanisława Skrzeczkowska Anna Kowalska-Noordam Mirosława Was Waldemar Marczewski 《Potato Research》1990,33(4):497-503
Summary To assess the reliability of dot-spot hybridization assay for the Potato Spindle Tuber Viroid, more than 1000 potato samples
were cross-tested by biological and hybridization methods. Both methods gave similar results with a range of cultivars. The
dot-spot hybridization test was reproducible with formaldehyde-denaturated cell-sap samples stored at room temperature for
over 6 months. This finding means that samples can be transported from field stations to a diagnostic laboratory and allows
for cross-checking of results between laboratories. The data suggest that dot-spot hybridization is suitable for breeding
and quarantine purposes. 相似文献
263.
Crystal J. Hoffman BS Lais R. Costa MV MS PhD DACVIM DABVP Lisa M. Freeman DVM PhD DACVN 《Journal of Equine Veterinary Science》2009,29(10):719-726
Nutrition is a critical component of equine health. Horse owners' knowledge of nutrition is likely to affect their feeding practices. The aim of this study was to survey feeding practices, dietary supplement use, and knowledge about equine nutrition in New England by surveying a subpopulation of horse owners (67/337 or 19.8%) who brought their horses to the Large Animal Hospital at the Cummings School of Veterinary Medicine at Tufts University between July and September 2008. All owners reported feeding hay, with the majority feeding grass or timothy hay. Most owners (96%) reported feeding a concentrate in addition to hay. Approximately 84% of owners reported including at least one dietary supplement in their horse's daily feeding. The most commonly used supplements were chondroprotectives, electrolytes, and multivitamins. Survey questions designed to assess the owner's knowledge of nutrition suggested that many owners may not have a basic understanding of principles of equine nutrition; less than 50% knew the daily water and hay requirements for a horse, and 69% lacked knowledge about the proper use of concentrates in a diet. Most of the surveyed owners consulted multiple sources of information concerning equine nutrition, including veterinarians (n = 36), trainers (n = 27), feed stores (n = 10), and the internet (n = 7). Although the major source of information was the veterinarian, it appears that the communication between horse owners and their veterinarian about optimal feeding practices could be enhanced. This survey demonstrated areas in the veterinarian–client dialog that need to be addressed when evaluating the health and well-being of the horse. 相似文献
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266.
Jin Zhao Qingwu Xue Kirk E. Jessup Thomas H. Marek Wenwei Xu Jourdan Bell 《Journal of Agronomy and Crop Science》2023,209(1):116-131
Deficit irrigation (DI) is an effective way to save irrigation water while maintaining sustainable yield in irrigated crops. However, limited information is available related to canopy structure and solar radiation use under DI condition. In this study, our objective was to assess maize hybrids for leaf development, photosynthetically active radiation (PAR) interception and water use under DI condition. Field experiments were conducted in 2016 and 2017 in four maize hybrids at well-watered (I100, referring to 100% evapotranspiration [ET] requirement) and DI (I75, referring to 75% ET requirement) water regimes. Compared to I100, I75 did not reduce maize biomass and grain yield. Although DI reduced the leaf appearance rates (1.5% in 2016 and 7.6% in 2017) and resulted in greater variations in leaf area index (LAI) among hybrids, the amount of PAR interception was not affected during the growing season. DI significantly reduced the seasonal ET in both years (19.8% in 2016 and 26.6% in 2017). All the hybrids extracted more soil water (29 mm in 2016 and 27 mm in 2017) at I75 than at I100. Maize plants at I75 had greater water use efficiency (WUE) (1.68 kg m−3) than those at I100 (1.41 kg m−3). However, DI did not affect radiation use efficiency (RUE). In conclusion, DI at I75 maintained grain yield through improved soil water extraction and WUE but stable canopy radiation interception and RUE. 相似文献
267.
Change in Sox9 protein localization through gonad development in Russian sturgeon (Acipenser gueldenstaedtii) 下载免费PDF全文
Maciej Kamaszewski Aleksandra Gosk Marek Skrobisz Teresa Ostaszewska 《Aquaculture Research》2017,48(6):3111-3120
The Russian sturgeon is a highly prized species reared in aquaculture. The process of gonad development in this critically endangered species is poorly understood. The aim of this study was to describe the localization of Sox9 protein during gonad development of the Russian sturgeon from the day of hatching to the 1440 day post hatching (dph). The larvae at age 1, 10, 25 dph and prepared gonads of 300, 720, 1440 dph individuals were immunohistochemistry‐stained for Sox9 detection. Sox9‐positive regions were detected in larvae in primordial germ cells cytoplasm. Analysis of 300 dph sturgeon gonads revealed the presence of the Sox9 protein in cytoplasm of some oocytes in the chromatin nucleus stage. In testes at 720 dph, Sox9 was observed in the cytoplasm of type A and early B spermatogonias. In the ovaries, Sox9 was observed in the cytoplasm of diplotene oocytes and prefollicular cells. In testes of 1440 dph sturgeon, Sox9 was present in the nucleus of the spermatocytes and in types A and B spermatogonias cytoplasm. Analysis of ovaries at 1440 dph reveals multiple diplotene oocytes with a Sox9‐positive cytoplasm. Furthermore, in 720 and 1440 dph, sturgeon presence of intersexual gonads was detected. In intersex gonads, Sox9 was observed in the cytoplasm of diplotene oocytes and type A spermatogonias. This study may be the first attempt to determine Sox9 protein localization during ontogenesis of the Russian sturgeon. Localization of Sox9 protein may become a useful marker of the maturation level in testis of the Russian sturgeon. 相似文献