除草剂莠去津降解菌SFAD3的分离鉴定及其土壤修复潜力

为获得能够修复除草剂莠去津污染土壤的高效降解菌,采用摇瓶富集法、平板分离法从莠去津过量使用的土壤中分离得到降解菌SFAD3,通过形态学、生理生化特征观察以及16S rDNA和ITS序列分析进行种类鉴定,测定获得菌株的最适降解条件,并通过土壤接种和盆栽试验验证菌株对莠去津污染土壤的修复作用。结果表明,菌株SFAD3最终被鉴定为门多萨假单胞菌Pseudomonas mendocina,该菌株培养30 d时对污染土壤中50 mg/kg莠去津的降解率可达72.6%;菌株SFAD3在MM液体培养基中最适降解条件为温度37℃、pH 7、莠去津初始浓度6.25 mg/L、接种量2%,对莠去津降的降解率为50.0%～72.2%;与仅有莠去津的处理相比,添加有SFAD3发酵液的处理20 d后芝麻的株高、根长、湿重和干重能够显著恢复,并且该菌对芝麻还具有一定的促生作用。表明降解菌SFAD3在修复莠去津污染土壤方面具有潜在的应用价值。     

曲拉通和硝酸混合溶液直接提取快速测定草鱼中铅含量

为建立快速测定草鱼中铅含量的方法,通过分析曲拉通和硝酸混合溶液浓度、提取时间、称样定容体积比例等因素的影响,以及基体改进剂的使用效果,确定了草鱼中铅元素提取测定的最佳条件。验证结果显示：采用曲拉通和硝酸混合溶液提取草鱼中铅元素方法的提取率为86.5%～95.6%,加标回收率为91.5%～95.6%,精密度均小于4.0%,检出限和定量限分别为0.011和0.032 mg/kg,满足了国家标准GB 5009.12—2017第一法石墨炉原子吸收光谱法的要求。与国家标准方法比较,本研究建立方法的样品前处理时间从4～12 h缩短至0.2 h内,酸试剂用量从湿法消解的7～12 mL减少至1～2 mL;前处理过程无需高温消解,无需其他前处理设备,避免了样品污染和待测元素损失,实现了降低分析成本以及保护环境和试验人员的目的。结果表明,该方法是一种简单、准确、绿色、快速测定草鱼中铅含量的方法,尤其适合大量样品检测。     

甜菜夜蛾(Spodopteraexigua Hübner)雄蛾触角对雌蛾性信息素的EAG反应

采用EAG生测技术研究表明,甜菜夜蛾雄蛾触角上具有4种性信息素组分(Z9,E12-14Ac(A)、Z9-14OH(B)、Z9-14Ac(C)和Z9,E12-14OH(D))的感受细胞.单一组分A,二元组分AB和AD,三元组分ABC、ABD和ACD及四元组分ABCD的EAG反应显著较高,与3头雌蛾性信息素腺体浸提液的反应强度相当.进一步对AB二元混合物中两组分的最佳比例以及最佳比例的剂量反应进行了测定.当AB间的比例为55时可产生最大的EAG反应,其它比例(91、73、37、19)间无明显差异.AB(55)混合物的使用剂量在0,04～4μg范围内,EAG值随剂量增加呈直线上升;使用剂量低于0.04μg或高于40μg,EAG值不再有明显变化.     

宁南旱区苜蓿草地土壤水分消耗规律及粮草轮作土壤水分恢复效应研究

对宁南旱区不同生长年限紫花苜蓿草地土壤水分消耗及粮草轮作水分恢复效应进行了研究。结果表明:(1)随着苜蓿生长年限延长,在1￣6年内苜蓿草地土壤湿度下降迅速,产草量逐年上升,7年后土壤湿度下降趋于平缓,但苜蓿产草量下降迅速,表明苜蓿生长强烈耗水引起深层土壤干燥化,导致苜蓿生长逐渐衰败,苜蓿平均降水生产效率逐年下降;(2)苜蓿草地土壤垂直剖面可分为降水入渗恢复层(0￣200cm)、根系发达枯竭层(200￣500cm)和根系衰老缓耗层(500cm以下)三个层次。随苜蓿生长年限延长,苜蓿剖面的主要土壤干层逐渐上移,并且干层厚度呈现减小趋势;(3)耕翻的苜蓿茬后轮作粮食作物的年份越长,土壤水分恢复越好,实行草粮轮作的苜蓿最迟不超过生长的第10年。     

农杆菌介导的马铃薯茎段遗传转化体系优化研究

以甘肃主栽品种的茎段为外植体,研究了不同转化条件(菌液浓度、感染时间、预培养及共培养时间)对转化效率的影响,以及选择压和培养基对抗性出芽的影响,初步建立了马铃薯品种“陇薯3号”和“台湾红皮”的遗传转化体系并获得了转化植株。卡那霉素生根筛选和PCR鉴定结果表明,再生的转化植株假阳性率比较高,要获得预期的转基因植株群体需加大遗传转化工作中转基因植株的数量。     

Measurements to Protect Owners'' Benefits under the Non-price Bottom Bidding

Investment on construction projects has become an important factor in improving the development of economy in China.The non-price bottom bidding is a representation that the investment on projects is becoming standardized.During the years of trying out the non-price bottom bidding,the colluding in bidding or bidding below the cost has happened several times,which have damaged the benefits of owners.To solve theses problems,this paper firstly studies the reason of these problems-the failure in the normal relationship between principal and agent.Then,some measures have been presented as follows: building systematic and scientific system of qualification prejudication,judging behaviors of colluding in bidding and bidding below the cost with reason and providing a surety mechanism of the project.The authors hope that these measures are beneficial to non-price bottom bidding.     

Strength and Microstructure of Sulphoaluminate Cement Containing Ultra- Fine CaCO3

The existence of ettringite in sulphoaluminate cement hardened paste is mainly in the form of pole-like crystal,which causes unfavorable effect on the cement.In this paper the strength and microstructure of the sulphoaluminate cement containing ultra-fine CaCO_3 weze discussed.Experimet results show that the strength of sulphoaluminate cement was obviously improved by adding 3% of ultra-fine CaCO_3.After curing for 28d,the compressive strength of cement paste and mortar is 100.6MPa and 94.1MPa respectively,and the bending strength of the mortar reached 12.5MPa.SEM study on hardened paste of sulphoaluminate cement containing ultra-fine CaCO_3 showed that its structure was dense and homogeneous,there was hardly any massive crystall detected.     

Analysis on Longitudinal Cracks and Deflection of the Pre-stressed V-shape Slab Roof Structure

In this paper, the finite element method is used to analyze the behavior of the prestressed V-shape Slab Roof structure, especially the effect of temperature change on the stress of slab. The calculated results indicate that when the temperature changes, the tensile stress at the lower extreme fibers of the flange plate near rib exceeds the tensile strength of concrete subjected to the combined action of triaxial stresses and results in the longitudinal cracks of the flange plate. The deflection analysis is based on the mechanical model of the prestressed V shape slab with the longitudinal cracks. The calculated results are in general agreement with the field observations.     

Determination to Weighting Coefficient of Combination Forecast Based on Rough Set Theory

The problem of determination to weighting coefficient is a key and difficulty for combination forecast. A method of determining weighting coefficient based on rough set theory is showed in this paper. Determining weighting coefficient is translated into estimating significance of attributes among rough set. A relation data model about combination forecast is established. Knowledge systems are built through making attribute value into eigenvalue. Under data moving, the weighting coefficients of a combination forecast model are computed by analyzing the dependence and significance of forecasting method for the predicted object. The proposed approach overcomes the subjectivity of traditional determination to weighting coefficient, avoids computing linear or nonlinear extremum problem and makes combination forecast more objective. The validity of the proposed approach is verified with a case.     

观赏羽衣甘蓝的离体繁殖

以花蕾为外植体，离体繁殖羽衣甘蓝８个杂各一代材料。培养基为（ｍｇ／Ｌ）：ＭＳ＋ＢＡ１－２＋ＮＡＡ０．１－０．２＋ＩＡＡ０．５＋（或不加）ＧＡ３０．３＋３％蔗糖。平均１８％－６７％的接咎花蕾产生不定芽；药有３０％的不定芽发育成完整植物。培养物的严重褐化和再生植物的普遍玻璃质化是影响植株成活的主要因素。培养基中加入活性炭和青霉素，并给以强光照射，可提高植株成活率。