南疆不同品种绵羊MSTN基因克隆及其组织表达谱分析

本研究旨在对山区型和田羊、平原型和田羊、卡拉库尔羊的MSTN基因进行组织表达谱分析。参考GenBank上（登录号：NM_001009428.1）绵羊MSTN基因序列设计引物,采用RT-PCR技术克隆绵羊MSTN基因序列,进行组织表达谱分析。结果表明：MSTN基因在各品种绵羊不同组织中表达的差异较大,MSTN在各品种绵羊的肌肉、肾脏和脾脏中都有表达,其中肌肉中表达量最高,其次是肾脏和脾脏;MSTN在山区型、平原型和田羊的心脏中表达丰度较低,而在卡拉库尔羊心脏中则几乎无表达;MSTN在山区型、平原型和田羊的肝脏中几乎无表达,而在卡拉库尔羊肝脏中则有一定量表达;MSTN在山区型和田羊、平原型和田羊与卡拉库尔羊的肺脏中则都无表达。     

高效减水剂和粉煤灰对混凝土性能的影响

通过对加入高效减水剂和粉煤灰的混凝土和易性和强度的试验研究,结果表明高效减水剂和粉煤灰都能提高混凝土的和易性;当粉煤灰掺量为25％、高效减水剂为1.0％时混凝土强度最大.     

两种植物冠层分析仪测量夏玉米LAI结果比较分析

植物冠层分析仪测量作物叶面积指数存在高估或低估现象,不同仪器的测量结果均与真实值存在差异,这种差异除了与仪器有关,还在不同的作物物候期表现出不同规律。本研究使用LAI-2000、Sunscan两种主流植物冠层分析仪测量夏玉米不同生育期LAI,并与LI-3000C叶面积仪实测结果比较。研究发现：当玉米LAI<1时,LAI-2000测量结果高于实际值,Sunscan测量结果与实际值无显著差异;12时,两种冠层分析仪测量结果均低于实际叶面积指数;不同LAI范围内,LAI-2000、Sunscan测量值与实际值的相关系数不同,最高可达0.9778和0.9637,通过建立测量值和实际值的经验关系模型,对仪器测量值进行校正可减少误差。     

黑龙江林蛙(Rana amurensis)消化道5-HT细胞的分布及形态学观察

应用ABC(avidin-biotin-peroxidase complex)免疫组织化学方法对黑龙江林蛙(Rana amurensis)消化道内的5-HT免疫活性细胞(5-Hydroxytryptamine immunoreactive cell,5-HT cell)进行了免疫组织化学的定位研究和形态学观察。结果显示:5-HT细胞在黑龙江林蛙消化道的各个部位均有分布,其中以胃幽门部分布密度为最高,胃贲门部次之,空肠部最低。5-HT细胞的形态多样,有圆形、椭圆形、锥体形和梭形等,细胞分布于上皮细胞之间、上皮基部和腺泡上皮细胞之间。5-HT细胞形态与其内、外分泌功能是相适应的。     

库尔勒香梨保鲜状况及贮藏中的新问题

库尔勒香梨是新疆特色的传统优势梨,不少学者认为库尔勒香梨是瀚海梨和鸭梨的自然杂交种。近年来,它已成为中国香港、东南亚市场上具有较强竞争力的水果。但是库尔勒香梨在贮藏过程中存在很多问题,严重影响了其销售量和经济效益,大大降低了商品价值,带来了较大的经济损失。     

增稠剂对特一粉冷冻面团拉伸特性的影响

以特一粉冷冻面团为研究对象,通过质构仪,研究添加黄原胶、卡拉胶、瓜尔豆胶、聚丙烯酸钠对冷冻面团拉伸特性的影响,通过单因素试验进行响应面分析。结果表明,当添加黄原胶为0.33%,瓜尔豆胶为0.90%,卡拉胶为0.61%,聚丙烯酸钠为0.05%时,得出最大拉伸面积,对冷冻特一粉面团品质有很好的改良作用。     

A rapid staining‐assisted wood sampling method for PCR‐based detection of pine wood nematode Bursaphelenchus xylophilus in Pinus massoniana wood tissue

For reasons of unequal distribution of more than one nematode species in wood, and limited availability of wood samples required for the PCR‐based method for detecting pinewood nematodes in wood tissue of Pinus massoniana, a rapid staining‐assisted wood sampling method aiding PCR‐based detection of the pine wood nematode Bursaphelenchus xylophilus (Bx) in small wood samples of P. massoniana was developed in this study. This comprised a series of new techniques: sampling, mass estimations of nematodes using staining techniques, and lowest limit Bx nematode mass determination for PCR detection. The procedure was undertaken on three adjoining 5‐mg wood cross‐sections, of 0.5 × 0.5 × 0.015 cm dimension, that were cut from a wood sample of 0.5 × 0.5 × 0.5 cm initially, then the larger wood sample was stained by acid fuchsin, from which two 5‐mg wood cross‐sections (that adjoined the three 5‐mg wood cross‐sections, mentioned above) were cut. Nematode‐staining‐spots (NSSs) in each of the two stained sections were counted under a microscope at 100× magnification. If there were eight or more NSSs present, the adjoining three sections were used for PCR assays. The B. xylophilus– specific amplicon of 403 bp ( DQ855275 ) was generated by PCR assay from 100.00% of 5‐mg wood cross‐sections that contained more than eight Bx NSSs by the PCR assay. The entire sampling procedure took only 10 min indicating that it is suitable for the fast estimation of nematode numbers in the wood of P. massonina as the prelimary sample selections for other more expensive Bx‐detection methods such as PCR assay.     

傣族新村景观特色初探——简评云南景洪曼景法村

云南省景洪市的曼景法村,是近年来在新农村建设中涌现出来的傣族新村。其景观特点与传统的傣族村寨相比有所区别,有自己的独特新颖之处。文章从建筑、植物、水景、宗教景观、道路、园林小品等方面对傣族新村曼景法的景观进行了分析。     

芥菜、苦荬菜的活体蔬菜保鲜研究

试验模拟植物组织培养或鲜花插枝保鲜的原理,对芥菜(Brassica juncea Czem.)和苦荬菜[Ixeris dentate(Thunb.)Nakai]的贮藏保鲜进行了试验研究。通过补充必要的水分养分,可以显著地改善与延长芥菜和苦荬菜贮藏保鲜的时间,贮藏期间的叶绿素、维生素C、pH值、亚硝酸盐等理化指标在贮藏6d的时期内未发生显著变化,而对照的相关指标在2～3d内即发生显著变化。说明此方法可以有效延长叶菜的贮藏保鲜时间,且营养价值和食用安全性不受影响。     

Cutaneous pythiosis in a dog from Brazil

This report describes a case of cutaneous pythiosis in a 6‐year‐old female mixed breed dog, from the central west region of São Paulo State, Brazil. The cytological and histopathological analyses showed an intense inflammatory infiltrate with presence of numerous hyphal elements, suggesting infection due to Pythium insidiosum. The diagnosis was confirmed by nested‐PCR, which was carried out with specific primers derived from the ribosomal DNA region. The pathogen occurs in Brazil and veterinarians should be aware of the importance of correctly diagnosing this disease and differentiating it from other fungal diseases.