3种滤食性贝类对塔玛亚历山大藻的摄食研究

20 0 3年 4月在实验室内采用静水法 ,研究了长牡蛎、紫贻贝、栉孔扇贝对塔玛亚历山大藻的清滤率 (CR)和摄食率 (IR) ,实验时间持续 3d ,水温 17℃。实验中每 1种贝都分别投喂 :(1)叉鞭金藻 (对照藻 ) ,(2 )叉鞭金藻 +塔玛亚历山大藻去藻过滤液 1.5L ,(3)叉鞭金藻 +塔玛亚历山大藻细胞。结果表明 ,塔玛亚历山大藻过滤液 ,对 3种贝的清滤率的无明显影响 (P >0 .0 5 ) ;3种贝对塔玛亚历山大藻和叉鞭金藻混合液的清滤率与对照组相比明显降低 (P <0 .0 1) ;而对塔玛亚历山大藻的摄食率和保留率 3d间无显著变化 ;实验期间 ,随时间的变化 ,牡蛎对混合液的清滤率变化最大 ,贻贝次之 ,扇贝则无明显著变化 (P >0 .0 5 ) ;如果以摄食率作为指标 ,贻贝在控制赤潮方面的潜力较大 ,值得今后进一步研究。     

广东省海水养殖贝藻类碳汇潜力评估

大型藻类和滤食性贝类可以直接或者间接吸收水体中的碳（C）,收获养殖产品形成了一个＂可移出的碳汇＂,提高了海域的碳汇潜力。文章从物质量评估和价值量评估两方面对广东省贝、藻养殖的碳汇贡献进行了定量评估。物质量评估结果显示,2009年广东省海水养殖的贝类和藻类收获可以从海水中移出C约11×104t,相当于39.6×104t二氧化碳（CO2）;价值量评估结果显示封存固定这些CO2所需要的费用约0.59×108～2.38×108美元。因此,基于贝、藻养殖的碳汇渔业具有巨大的经济效益、生态效益和社会效益。     

Lipophilic Toxins in Wild Bivalves from the Southern Gulf of California,Mexico

Most of the shellfish fisheries of Mexico occur in the Gulf of California. In this region, known for its high primary productivity, blooms of diatoms and dinoflagellates are common, occurring mainly during upwelling events. Dinoflagellates that produce lipophilic toxins are present, where some outbreaks related to okadaic acid and dinophisystoxins have been recorded. From January 2015 to November 2017 samples of three species of wild bivalve mollusks were collected monthly in five sites in the southern region of Bahía de La Paz. Pooled tissue extracts were analyzed using LC-MS/MS to detect lipophilic toxins. Eighteen analogs of seven toxin groups, including cyclic imines were identified, fortunately individual toxins did not exceed regulatory levels and also the total toxin concentration for each bivalve species was lower than the maximum permitted level for human consumption. Interspecific differences in toxin number and concentration were observed in three species of bivalves even when the samples were collected at the same site. Okadaic acid was detected in low concentrations, while yessotoxins and gymnodimines had the highest concentrations in bivalve tissues. Although in low quantities, the presence of cyclic imines and other lipophilic toxins in bivalves from the southern Gulf of California was constant.     

Seasonal differences in effect of broodstock diet on spawning success in the great scallop

Great scallop (Pecten maximus L.) broodstock collected at two different seasons, spring (May) and winter (December), were given four different diets during conditioning. The spring group was spawned in late June, and the winter group in February.The highest number of veligers in the summer spawning (30 millions per 10broodstock), was produced using a diet consisting of 80% Tahitian Isochrysis galbana. In the winter spawning, broodstock given a dietconsisting of 80% diatoms (Skeletonema costatum/Chaetocerosgracilis) produced the highest number of veligers (29 millions per 10broodstock). Fecundity and the number of egg-releasing individuals were higher in the early summer spawning (June) than in the winter spawning (February), however, the total number of three days old veligers produced was nearly the same in the two seasons.     

Scallop introductions and transfers, from an animal health point ofview

The effects of transfers and introductions of bivalue molluscs are alwaysmore or less unpredictable. Moving scallops, there is a risk of introducingpathogenic agents or of disturbing the balance between potentiallypathogenic agents and host species in the recipient ecosystem. Risk is noteliminated by merely following official regulations. Some factors which maybe important in order to assess risk, and to choose the level of risk we arewilling to accept, are: The existence of ``stowaways', and the action of mechanical vectors.One organism may carry another, and it is seems impossible to obtain a``clean' organism, in spite of long quarantines. The biological vector function. There may be unknown reservoirs,intermediate or alternative hosts of pathogenic agents in the ``new'environment. An introduced species may also carry potentially pathogenicagents into a new ecosystem. The often lacking knowledge concerning epizootiology. Life cycles ofmany pathogenic agents are poorly known, as are the infectious dose ofagent, influence of environmental factors on disease, etc. The diagnostic tools, and the ``human factor' in diagnostics. Methodsare often not sensitive enough to detect a pathogenic agent in a carrierstate, or immediately after the agent has entered the host, or at a lowprevalence/how intensity level. It is also important that personnel aresufficiently trained to do the diagnostic work. The official priority of disease control. There are no notifiablediseases of scallops. EC regulations, and the ``one area/one agent/one bivalve species'management. National and international trade, including illegal transport of livebivalves.Considering these factors enables a better focus on the riskfactors and the assessment of risks associated with introductions andtransfers of scallops. Consideration may also help to clarify the scientificlevel of knowledge and experience required to handle scallop diseases, andfocus on practical problems in scallop health control.     

5种滤食性贝类对牙鲆的粪便、残饵及网箱养殖区沉降物的摄食行为

采用室内静水法,研究了虾夷扇贝、栉孔扇贝、长牡蛎、紫贻贝及菲律宾蛤仔对不同浓度、质量的牙鲆粪便、残饵、沉降物的摄食行为,从摄食生理角度,分析了基于滤食性贝类的鱼+贝IMTA养殖模式的可能性。研究结果显示,这5种贝类对鱼粪、残饵及网箱周围沉降物都可摄食,但是,不同贝类对食物可获得性的反应不同。菲律宾蛤仔、长牡蛎、栉孔扇贝的摄食率显著高于其它2种贝类;5种贝类的吸收率、吸收效率无显著性差异。不同饵料对贝类的摄食行为影响显著。吸收效率(AE)都随有机物含量的增加而增大;对有机物的吸收率(AR)与有机物浓度(POM)呈线性正相关关系。颗粒物TPM浓度达到26.24,21.64,27.00 mg/L时,长牡蛎、栉孔扇贝、虾夷扇贝会产生假粪来调节摄入的能量。在牙鲆+滤食性贝类的IMTA养殖模式中,菲律宾蛤仔、长牡蛎、栉孔扇贝是比较好的候选种。     

双壳类功能基因和蛋白质组学研究进展

近十年来,双壳类在水产业中的地位日趋重要。由于对赤潮的发生存有预警作用,其在环境科学中的价值日益受到重视。有关双壳类基因组学和蛋白质组学的研究进展很快。上世纪,双壳类基因组结构、功能和进化等方面的研究才刚刚开始,现在已经开始研究双壳类基因连锁图谱、基因转录和蛋白质组学。与双壳贝类生产性能相关的分子生物学特性研究报道越来越多。主要的研究热点是双壳类重大疾病的易感性、双壳类对环境压力的耐受力以及生长状况的分子基础等。太平洋牡蛎（Crassostreagigas）是目前双壳类基因组关注的焦点,被推荐为首选的双壳类基因组学模式动物,正在筹划对其进行全基因组测序。明确了双壳类在污染监测中的作用,可以从双壳类细胞和机体与共息生物之间的关系来进一步研究,由此扩展到浮游植物的毒理效应领域的基因组研究。从养殖增殖考虑,今后需重点对双壳类的生长、性别决定和生殖腺发育等方面的基因调控以及幼虫阶段的某些基本过程的基因控制方面进行研究,进而推广到实际生产应用中。     

Environmental DNA as a monitoring tool for the endangered freshwater pearl mussel (Margaritifera margaritifera L.): a substitute for classical monitoring approaches?

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