康拜尔早生葡萄未熟种子内源GAs的抽提及活性GAs组成的分析

用高效液相色谱（ODS－HPLC）对康拜尔早生葡萄未熟种子提取液分离成33段（No．1～33）每段用矮生稻测定内源GAs的活性含量。测定表明，具有活性的GAs主要在No．14～15段（A区）、No．18～19（B区）、No．23～25段（C区）和No．26～27段（D区），分别含有相当于GA_3活性量，0．46、0．33、1．00、10．39 μg／kg FW。对D区用GC－MS全扫描质谱鉴别出，D区内的GAS主要成分为GA4、3epi－GA4、GA4－15－ene、GA7、GA7－15－ene和GA_34，并没发现GA_3的存在。     

Floral induction (FI) in longan (Dimocarpus longan, Lour.) trees. III: Effect of shading the trees on potassium chlorate induced FI and resulting hormonal changes in leaves and shoots

Previous experiments demonstrated that treatment of longan trees with potassium chlorate (KClO₃) induces “off season floral induction” (FI) even in the absence of the naturally required cool temperature [Manochai, P., Sruamsiri, P., Wiriya-alongkorn, W., Naphrom, D., Hegele, M., Bangerth, F., 2005. Year around off season flower induction in longan (Dimocarpus longan, Lour.) trees by KClO₃ applications: potentials and problems. Sci. Hortic. 104, 379–390]. Potassium chlorate, however, cannot replace the presence of functional mature leaves and sufficient light intensity. Here we examined in more detail the effect of shade (about 10% of natural sunlight) on KClO₃ affected hormone concentrations/transport of leaves and shoot apical buds (SAB) and their interactions with FI.     

苹果受精着果机理研究初报

用气相色谱（ＧＣ）测定了ＰＰ３３３，ＧＡ３处理及未处理苹果果实，果台副梢中内源ＧＡ３，ＧＡ４＋７，ＡＢＡ，ＩＡＡ，Ｚ和ＺＲ的含量，用质谱（ＭＳ）测定了＾１５Ｎ的分配运转。结果表明，ＰＰ３３３处理明显地增加主要座果率，抑制果台副梢的增长。而ＧＡ３则具有增大果径和促进果台副梢生长的作用，内源细胞分裂素的含量与苹果能力相吻合，其它激素含量的多少与着果没有是有显相关。多效唑（ＰＰ３３３）处理明显地提高了内     

一类新型植物生长延缓剂的作用机理及其应用

含有降冰片烯二氮杂环丁烯、三唑、嘧啶、吡啶和咪唑类结构的一类新型植物生长延缓剂,其共同的特征是在分子内杂环中的 sp~2杂化 N 上具有一对孤对电子。这对孤对电子作为第六配体结合到细胞色素 P-450内血红素铁上,代替了催化反应中所需的氧,导致某些依赖细胞色素P-450的单氧酶失活,并因此影响与 GA,ABA 和甾醇等有关的代谢途径,进而调节植物的生长和发育过程。因此,这类植物生长延缓剂在农林和园艺等方面具有广泛的应用前景。     

樟树、檫树、闽楠种子的休眠和萌发特性

对樟科Lauraceae 3个属的代表性树种的种子休眠与萌发特性进行研究．结果表明:新采集的樟树Cinnamoumcamphora (linn．)Presl、檫树Sassafras Tzumu(Hemsl．)Hemsl和闽楠Phoebe bournei(Hemse．)Yang的种子胚形态发育正常,除檫树种子外,樟树和闽楠种子的离体胚能正常萌发,在黑暗条件下,3个树种的完整种子于15-35℃温度范围内,无论恒温和变温均不能萌发,闽楠种子仅在光照下30、35℃恒温和30℃日／20℃夜变温中较窄的温度范围内能萌发,但发芽迟缓、过程长、萌发率低;3个树种的种子均具生理休眠特性,种皮透气性差和存在发芽抑制物质是樟树种子休眠的原因,低温(5℃)层积80 d,不能完全解除休眠,在30℃日／20℃夜最适变温条件下．并给予适当光照,萌发率仅为41．00％;采用0．5％KNO3+0．10％GA3溶液浸种2 h后低温层积80 d,在光照条件下,萌发率可提高到74．00％;胚存在发芽抑制物质和种皮透气性差是引起檫树种子休眠的最初原因,经低温层积后,种皮防碍抑制物质向外渗透是种子不能完全解除休眠的关键因素,低温层积240 d,在25℃日／15℃夜最适温度下,光暗中的萌发率仅为59．00％-54．00％．而采用4％H2O2+0．10％GA3溶液浸种后低温层积．萌发率可高达87．00％;种皮透气性差,发芽要求特殊的温度和一定的光照是闽楠种子休眠的原因,低温层积60 d,在光照条件下,20、25℃恒温和30℃日／15℃夜变温适宜温度中,萌发率能达77．33％-85．33％．     

Study on the Change of GAs,ABA Level and ABA／GAs Ratio in the Embryos of Spring Wheat and Winter Wheat During Vernalization

Spring wheat(cv.7742)and winter wheat (cv.Nongda 015) were vernalized by seed chilling,and the changes of GAs,ABA content and GAs/ABA ration in embryo wer investigated.The differences between cultivars,treatments and variety-treatment inleractions were significant for both GAs and ABA.The level of GAs and ABA as well as GAs/ABA ratio were hardly changed by seed chilling in spriug wheat Howerve,in winter wheat,the GAs content decreased much less compared with ABA that reduced sharply after chilling.It showed that the GAs/ABA ratio in winter wheat was increased remarkably(4.55 times)than in spring wheat.It was suggested that the absolute GAs Ievel was independent of the vernalization effect because there was a higher GAs Level before and after seed chilling in winter wheat when compared with spring wheat,One of the vemalization effects was the decline of ABA level that led to the increase of GAs/ABA ratio.     

NtCPS2对烟草光合特征及光合产物的影响

为探讨顺-冷杉醇合成基因NtCPS2对烟草光合作用的影响,以烤烟品系8306、8306B(纯合突变植株T3代)、新豫烟11号(MSK326×8306B)和烤烟品种MSK326、豫烟11号(MSK326×8306)为试验材料,比较其顺-冷杉醇、赤霉素、脱落酸含量及相关合成基因相对表达量,光合参数及光合作用相关基因 相对表...     

ABA/GAs调控特异蛋白质与柑桔的抗寒性

选用较抗寒柑桔品种兴津温州蜜柑(Citrus unshiu Marc.)枳砧盆栽苗为材料,研究了人工低温锻炼和外源 ABA喷布处理下其内源 ABA/GAs对抗寒特异蛋白质的调控作用及与抗寒性的关系。两种处理均诱导兴津柑桔苗抗寒力的发育。在抗寒力发育过程中,叶片内源ABA、ABA/GAs在第7天呈一峰状剧增趋势。同时,其基因表达发生改变,第7到21天,新产生了32、25KD两种可溶性特异蛋白质及104、37、30KD3种膜特异蛋白,第14和21天产生17和82、27KD3种膜特异蛋白,它们在17～28℃脱锻炼3天后均消失。作者提出低温、ABA首先诱导ABA/GAs的积累,由ABA/GAs启动特异性板机,最终合成抗寒特异蛋白质,进而提高柑桔之抗寒力;膜特异蛋白质贡献可能更大。     

An application of Neurogenetic Algorithm System to individual tree growth model

A neural network system with genetic algorithms (Neurogenetic Algorithm System, or NGAS) was employed to develop individual coniferous tree growth models. A multivariable regression model was applied to compare the performance of NGAS. An IBM personal computer with the BioComp System’s software program of NGO was used to execute this comparison. The results indicate that NGAS is more accurate and effective than the conventional regression method in modeling individual tree growth based on the criteria of Sum of Squared Error (SSE), Average Absolute Error (AAE), Mean Squared Error (MSE) and Final Predicted Error (FPE). This study also suggests that individual tree growth may indeed be a non-linear process. Using this flexible neural network system to model individual tree growth can yield satisfactory prediction results.     

稻株内源iPA和GAs变化与再生芽的关系

采用中国农业大学提供的植物激素ELISA方法,研究了杂交水稻中后期各器官中内源激素iPA和GAs的变化及其与再生芽萌发生长的相关性。结果表明,内源激素iPA,GAs变化与再生芽萌发生长关系密切;头季稻齐穗后5d至19d植株内源激素iPA,尤其是GAs的含量较低,而稻穗中内源激素含量水平相对较高,再生芽生长缓慢或处于休眠状态;齐穗19d以后,随着稻穗中内源激素含量水平的降低,稻株叶、鞘和茎、芽中内源促进类激素iPA,GAs含量水平的增加,再生芽萌发、伸长的速度相应加快;齐穗后19d下部节茎中iPA,GAs含量水平高于上部节茎,低位芽长于高位芽;齐穗后26d,33d上部节住茎、芽的内源iPA,GAs整体含量高于下部节位,上位芽生长速度快于下位芽。表明在一定条件下提高内源激素的整体含量,能打破再生芽的休眠,促进其萌发生长。