EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide,Inhibits Liver Cancer Cell Growth and Adhesion

The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.     

A comparison of the effects of carbon dioxide and medical air for abdominal insufflation on respiratory parameters in xylazine-sedated sheep undergoing laparoscopic artificial insemination

AIMS: To determine if abdominal insufflation with medical air will improve oxygenation and ventilation parameters when compared to insufflation with CO₂ in xylazine-sedated sheep undergoing laparoscopic artificial insemination (AI).

METHODS: Forty-seven sheep underwent oestrus synchronisation and were fasted for 24 hours prior to laparoscopic AI. Each animal was randomised to receive either CO₂ or medical air for abdominal insufflation. An auricular arterial catheter was placed and utilised for serial blood sampling. Respiratory rates (RR) and arterial blood samples were collected at baseline, after xylazine (0.1?mg/kg I/V) sedation, 2 minutes after Trendelenburg positioning, 5 minutes after abdominal insufflation, and 10 minutes after being returned to a standing position. Blood samples were collected in heparinised syringes, stored on ice, and analysed for arterial pH, partial pressure of arterial O₂ (PaO₂), and CO₂ (PaCO₂). The number of ewes conceiving to AI was also determined.

RESULTS: Repeated measures ANOVA demonstrated temporal effects on RR, PaO₂, PaCO₂ and arterial pH during the laparoscopic AI procedure (p<0.001), but no difference between insufflation groups (p>0.01). No sheep experienced hypercapnia (PaCO₂>50?mmHg) or acidaemia (pH<7.35). Hypoxaemia (PaO₂<70?mmHg) was diagnosed during the procedure in 14/22 (64%) ewes in the CO₂ group compared with 8/23 (35%) ewes in the medical air group (p=0.053). Overall, 15/20 (75%) ewes in the CO₂ group conceived to AI compared with 16/22 (72.7%) in the medical air group (p=0.867).

CONCLUSIONS AND CLINICAL RELEVANCE: There were no statistical or clinical differences in RR, PaO₂, PaCO₂, pH, or conception to AI when comparing the effects of CO₂ and medical air as abdominal insufflation gases. None of the sheep experienced hypercapnia or acidaemic, yet 42% (19/45) of sheep developed clinical hypoxaemia, with a higher percentage of ewes in the CO₂ group developing hypoxaemia than in the medical air group. Based on the overall analysis, medical air could be utilised as a comparable alternative for abdominal insufflation during laparoscopic AI procedures.     

高抗氧化活性lager型啤酒酵母选育

采用过氧化氢刺激lager型啤酒酵母,Tadpoling法筛选细胞活力恢复较快突变菌,根据各菌株线粒体膜电位、胞内氧自由基(ROS)水平筛选获得4株活性较高菌株。比较突变菌与原始菌传代发酵性能、胞内ROS、活力指标及每一代细胞死亡率水平,最终获得一株抗氧化能力提高且活力稳定性较高啤酒酵母菌株。分析验证突变菌线粒体DNA修复相关基因MHR1发现,该基因发生部分碱基突变。     

超高效液相色谱—串联质谱法检测鸡血液样品中喹诺酮类药物残留

旨在建立鸡血液样品中多种喹诺酮类药物残留的超高效液相色谱—串联质谱方法。样品经乙腈提取,经Waters Acquity UPLC BEH C₁₈色谱柱分离,以甲醇和0.1%甲酸水溶液为流动相进行梯度洗脱,电喷雾正离子（ESI⁺）模式电离,MRM扫描模式检测。经方法学验证,该方法的线性关系、回收率、精密度均符合要求。应用超高效液相色谱—串联质谱法建立了鸡血液样品中多种喹诺酮类药物同时检测的方法,实现了动物血液样品中12种喹诺酮类药物同时定性、定量分析。     

低、无毒农药防治花灌木主要刺吸式口器害虫

在查清天津市园林主要花灌木刺吸口器害虫桃粉蚜、绣线菊蚜、棉蚜和山楂红蜘蛛发生消长规律的基础上 ,确定了防治适期和结合点 ,筛选出 10 %吡虫啉、绿灵、蚧螨灵、 1. 8%齐螨素和花保5种低、无毒药剂 ,防效均达到 90 %以上。查出天敌 13种 ,并对优势种作了取食量调查     

安普霉素对仔猪内分泌的调控作用及血液生化指示的影响

采用单因子试验设计 ,28日龄大长北三元杂交断奶仔猪72头随机分为3组 ,研究饲料中添加不同剂量的安普霉素 (0、20、90mg/kg)对仔猪内分泌的调控作用及血液生化指标的影响。试验期为4周。结果表明 :仔猪日粮中添加90mg/kg的安普霉素可促进机体与生长有关的内分泌活动 ,提高内源激素 (生长激素、胰岛素、甲状腺激素T3)水平 (P<0.05),从而促进肌肉蛋白沉积 ;并具有显著降低血液中氨、尿素氮含量和提高血糖水平的作用 (P<0.05) ,表明安普霉素对仔猪具有增加氮沉积 ,促进蛋白质合成、抑制蛋白质分解的作用     

3种农药的微核效应研究

运用蚕豆根尖微核技术研究3种农药(甲胺磷、甲基硫菌灵和盐酸吗啉胍)在不同浓度下的诱变效应。结果表明,3种农药诱发蚕豆根尖细胞微核率与对照组相比均有不同程度的上升(P<0.05或P<0.01),农药浓度与微核率呈剂量-效应关系,盐酸吗啉胍的诱变效应大于其他2种农药。     

37 ℃持续热应激肉鸡血气改变的动态分析

80只25日龄雄性AA肉鸡,随机分为对照组(21～24 ℃,相对湿度50%±5%)和试验组(37 ℃±0.5 ℃,相对湿度70%±5%),自由采食和饮水.在12 d的试验期间,动态检测血气指标、呼吸频率、直肠温度和生产性能的变化.结果显示热暴露初期呈现代偿性呼吸性碱中毒,其特点为动脉血二氧化碳分压(3.82±0.36)降低,pH(7.48±0.04)升高;中期呈现失代偿性代谢性酸中毒,其特点为血浆HCO-3浓度(19.00±2.55)降低,pH(7.37±0.06)显著下降(P＜0.05);后期酸碱平衡趋于正常.热应激后,肉鸡日增重和日采食量极显著下降(P＜0.01)、料肉比极显著升高(P＜0.01);呼吸频率极显著升高(P＜0.01);热暴露第1、5、7、9和12天体温极显著升高(P＜0.01).动脉血氧分压与呼吸频率呈极显著负相关(P＜0.01),与体温呈显著负相关(P＜0.05);血氧饱和度与体温极显著负相关(P＜0.01).表明37 ℃持续热应激的肉鸡呈现由代偿性呼吸性碱中毒转变为失代偿性代谢性酸中毒,最后趋于正常的动态变化过程.