超高效液相色谱—串联质谱法检测鸡血液样品中喹诺酮类药物残留

旨在建立鸡血液样品中多种喹诺酮类药物残留的超高效液相色谱—串联质谱方法。样品经乙腈提取,经Waters Acquity UPLC BEH C₁₈色谱柱分离,以甲醇和0.1%甲酸水溶液为流动相进行梯度洗脱,电喷雾正离子（ESI⁺）模式电离,MRM扫描模式检测。经方法学验证,该方法的线性关系、回收率、精密度均符合要求。应用超高效液相色谱—串联质谱法建立了鸡血液样品中多种喹诺酮类药物同时检测的方法,实现了动物血液样品中12种喹诺酮类药物同时定性、定量分析。

Determination of Quinolones Residues in Chicken Blood Samples by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

The objective of this study was to establish an ultra performance liquid chromatography-tandem mass spectrometry method for the determination of various quinolones residues in chicken blood samples. The samples were extracted with acetonitrile solution and were separated by Waters Acquity UPLC system with a BEH C₁₈ column. The used mobile phases were methanol and 0.1% formic acid aqueous solution in gradient elution. The electrospray was operated in the positive ionization mode, and the compounds were identified by MRM mode. Methodological identification showed that the linear relationship, recovery and precision of the established method met the requirements. A method for simultaneous determination of various quinolones residues in chicken blood samples by ultra performance liquid chromatography-tandem mass spectrometry was successfully established, and qualitative and quantitative analysis of 12 quinolones residues was achieved simultaneously.