茶树花青素形成分子机理的研究进展

花青素是由类黄酮合成途径产生的次生代谢产物,含量高时会使茶树新梢呈现红色或紫色。同时,花青素相比儿茶素等具有更明显的抗氧化、预防肿瘤等药理保健作用。文章就茶树花青素合成途径、转录及转录后调控等方面进行综述,以期更好地为高花青素茶树的育种研究提供理论依据。     

Effect of cGMP on voltage-gated potassium channel in pulmonary artery smooth muscle cells from rats exposed to chronic hypoxia

AIM: To investigate the effect of cGMP on voltage-gated potassium channel in pulmonary artery smooth muscle cells (PASMCs) from rats exposed to chronic hypoxia. METHODS: (1) Wistar rats were randomly divided into control group (group A) and chronic hypoxia group (group B). Then group B received hypoxia 8 hours per day for 4 consecutive weeks. (2) Single PASMC was obtained via acute enzyme separation method. (3) Conventional whole-cell patch clamp technique was used to record resting membrane potential (Em) and ion currents of voltage-gated potassium channel. The changes of ion currents of voltage-gated potassium channel before and after applying cGMP (1 mmol/L), an agonist of protein kinase G (PKG), and cGMP plus H-8 (1 mmol/L), an inhibitor of PKG were compared between two groups. RESULTS: The Em of group B were significantly lower than that of group A. The ion currents of voltage-gated potassium channel in group A and group B were all significantly inhibited by cGMP [control group: from (118.0±5.0) pA/pF to (89.9±16.5) pA/pF, n=6, P＜0.05;chronic hypoxia group: from (81.0±5.0) pA/pF to (56.8±9.1) pA/pF, n=6, P＜0.05]and these effects were reversed by H-8 [control group: from (119.2±10.3) pA/pF to (117.8±9.1) pA/pF, n=6, P＞0.05;chronic hypoxia group: from (96.8±6.2)　pA/pF to (98.0±2.2)　pA/pF, n=6, P＞0.05]. CONCLUSIONS: The currents of voltage-gated potassium channel was inhibited by chronic hypoxic. The inhibitory effect of cGMP on currents of voltage-gated potassium channel in PASMCs from both normal and chronic hypoxic rats may be probably through the phosphorylation of voltage-gated potassium channel.     

富铁酵母对家兔红细胞免疫功能的影响

为探讨富铁酵母对家兔红细胞免疫功能的影响,将32只家免随机分成富铁酵母高、低剂量组,FeSO4组及空白对照组,分别灌服富铁酵母(Fe元素8 mg/kg、4 mg/kg)、FeSO4(Fe元素4 mg/kg)及空白生理盐水;连续5 d后,检测红细胞C3g受体花环率和复合物(IC)花环率.结果:富铁酵母较FeSO4及空白组能显著提高红细胞C3h受体花环和红细胞复合物(IC)花环的百分率,但高、低剂量间无显著性差异.结论:富铁酵母能显著提高红细胞免疫功能.     

酒精生理盐水合剂麻醉对家兔的影响

为探讨酒精生理盐水合剂的麻醉效果,观察了10只家兔注射40%的酒精生理盐水合剂麻醉前后的心率、呼吸、体温的变化。结果表明,酒精生理盐水合剂可以使兔呼吸频率降低,心率升高,体温下降;酒精生理盐水对家兔麻醉效果较好。     

吴茱萸中总皂苷、总黄酮和总酚酸的含量测定

目的 测定吴茱萸生药和提取物中总皂苷、总黄酮和总酚酸的含量.方法 采用80%乙醇提取,正丁醇萃取,乙醚脱脂的方法提取吴茱萸;用分光光度法测定总皂苷、总黄酮和总酚酸的含量.结果 实验用的两种吴茱萸生药中总皂苷、总黄酮和总酚酸的百分含量分别为5.78、5.24、30.23和12.36、10.99、24.36;提取物中总皂苷、总黄酮和总酚酸的百分含量分别为29.56、52.14、81.88.结论 不同吴茱萸药材中总皂苷、总黄酮和总酚酸的含量差异显著(t检验,P<0.01);吴茱萸提取物中含有大量的皂苷类、黄酮类和酚酸类成分.     

高效丰产水稻品种的群体生产特性研究

水稻轻简化栽培要求在较短的生育期获得相对较高的产量,对水稻物质生产的效率提出更高的要求.选择不同类型的杂交稻和常规粳稻品种,对物质生产特征及其生理基础进行分析,探讨高效丰产水稻品种的群体生产特性.结果表明:产量表现较好的现代品种高效和丰产能协同形成,结实期物质日生产量较高,稳定表现在200 kg·hm-2以上.高效丰产...     

江苏盐城首次记录到彩鹮

2020年5月4日在江苏盐城大丰麋鹿国家级自然保护区(120°47′—120°53′E,32°59′—33°03′N)记录到3只彩鹮。栖息地为一处浅滩水塘,3只彩鹮于水中觅食。以往曾宣布彩鹮在国内绝迹,本次记录填补了彩鹮在本地区的分布空白,证实了彩鹮在江苏省仍有分布。这一纪录或许说明本地区栖息地得到了有效的改善。     

普通高校女大学生初学健美操应注意问题的分析与探讨

当今,我国高校体育教学不断深入改革与创新,大学体育课不断的完善,健美操已成为当今女大学生的一种时尚运动,在校园内很受欢迎。对普通高校女大学生在初学健美操阶段应注意的问题进行了分析探讨,并提出了相应的建议。     

Physiological response of flag leaf and yield formation of winter wheat under different spring restrictive irrigation regimes in the Haihe Plain,China

In order to identify the optimum period of spring water-restrictive irrigation for winter wheat(Triticum aestivum L.) in the Haihe Plain, China and elucidate its effects on flag leaf senescence and yield formation, field experiments were conducted at the Xinji Experimental Station of Hebei Agricultural University from 2016 to 2019 by using different irrigation regimes in spring, including the conventional regime involving two irrigation periods(control(CK), the 3-leaf unfolding stage and the anthesis stage) and a series of single, restrictive irrigation regimes(SRI) comprising irrigation at the 3-leaf unfolding stage(3 LI), 4 LI, 5 LI, and 6 LI. There are five major findings:(1) The senescence(determined by the green leaf area, GLA) in the 4 LI treatment occurred moderately earlier than that in CK, showed no significant difference with that in 5 LI and 6 LI, and occurred significantly later than that in 3 LI.(2) Compared with other SRI treatments, the GLA value and photosynthetic rate in 4 LI were 14.82 and 20.1% higher, respectively. Microstructural analysis of flag leaf also revealed that the mesophyll cells and chloroplasts were irregularly arranged under drought stress in 3 LI and 6 LI; however, drought stress had minimal negative effects on the microstructure in 4 LI and 5 LI.(3) Postponed irrigation in spring could significantly increase superoxide dismutase(SOD) and catalase(CAT) activities in the early stage of grain filling; however, these activities would subsequently decrease. Among the four SRI treatments, the overall enzyme activities were the highest in 4 LI, and the combined malondialdehyde(MDA) content in flag leaves in 4 LI and 5 LI was 14.5% lower on average than that in 3 LI and 6 LI.(4) The soluble sugar(SS) and proline(Pro) contents in 4 LI were the highest among the four SRI treatments; however, they were lower than those in CK. The abscisic acid(ABA) hormone content in 4 LI and 5 LI was lower than that in 3 LI and 6 LI, respectively, suggesting a smaller drought stress effect in 4 LI and 5 LI.(5) In two growing seasons, there was a larger number of spikes per unit area in 4 LI(i.e., 13.4% higher than that in 5 LI and 6 LI) and the 1 000-grain weight in 4 LI was the highest among the four SRI treatments(i.e., 6.0% higher than that in the other three SRI treatments). Therefore, a single restrictive irrigation regime at the 4-leaf unfolding stage is recommended to be effective in slowing down the senescence process of flag leaves and achieving high yield.     

IBV AH1-99分离株N基因的克隆及序列分析

利用RT-PCR技术成功扩增出IBV AH1-99分离株的N基因全长cNDA,将其克隆到pMD18-T载体上。经酶切分析、PCR鉴定及核苷酸序列测定,成功获得该分离株N基因的重组质粒。序列分析结果表明,分离株N基因全长1 230个核苷酸,编码409个氨基酸。同部分IBV参考毒株相比,核苷酸同源性为85.8%~89.9%,氨基酸同源性为86.6%~91.0%,在进化关系树中,AH1-99与国内分离株X、LX4亲缘关系较近。