猪弓形虫病的有效治疗方案研究及分析

弓形虫病是一种人畜共患病,其分布广泛且危害极大。研究弓形虫病的防治办法不仅对公共卫生的治理有重要意义,对猪的养殖生产也非常重要。猪弓形虫病在猪身上的发病率很高,近年来对养猪业造成严重的影响。笔者将针对猪弓形虫的特点对有效治疗方案进行分析研究。     

猪弓形虫病的诊断与防治

弓形虫病也称弓形体病,是由刚第弓形虫引起的一种人兽共患原虫病,分布非常广泛。弓形虫是1908年第一次从北非的一种梳趾鼠的单核细胞中发现的,根据形态将其命名为弓形虫。之后,我国境内也发现了弓形虫,尤其是在一些家畜（类似于狗、兔等）的体内发现了弓形虫的存在。近年来,猪弓形虫病在猪群之间的暴发性传播给养猪业造成了较大损失,也使人们重新关注猪弓形虫病的研究。     

猪弓形虫病的诊断和防治

猪弓形虫病是一种人畜共患病,是由刚第弓形虫所引起的一种原虫病。猪弓形虫病的传染性强,一旦猪暴发可使整个猪场的猪只发病,无疑会给猪场带来巨大的经济损失。该病分布非常广泛,死亡率可达60%以上,猪场应予以高度重视。本文对猪弓形虫病的流行病学、临床症状、诊断和防治方法等进行了概述。     

犬场暴发弓形虫病的诊治

<正>弓形虫病是一种广泛存在、人畜共患、寄生于细胞内的原虫病,具有广泛的自然疫源性。犬弓形虫病一般处于隐性感染,当机体免疫力下降时才发病,其临床症状与犬瘟热非常相似,极易被误诊,应该引起广泛重视。     

免疫学技术在弓形虫检测方面的研究概况

弓形虫病是世界分布最广泛的一种人兽共患病,此病在温暖潮湿的地区感染率极高,所以建立高效敏感的检测方法是非常必要的。目前,国内检测弓形虫病的技术有多种,其中以免疫学技术为主。虽然病原学检测方法的敏感性高,特异性强,但其成本高,对人体会产生严重的副作用,所以并不适宜推广。近年来,通过对弓形虫表面抗原的大量研究,检测弓形虫病的免疫学技术也有了显著提高。从敏感性差、操作复杂到现在的特异性强、快速简便,从单一抗原的检测到现在的多表面抗原的检测,无疑为检测弓形虫带来了新的思路。论文主要对检测弓形虫的ELISA、改良凝集试验以及化学发光免疫分析的最新进展进行了综述。     

人工感染小白鼠弓形虫病攻毒量的测定

<正>弓形虫病(Toxoplasnasis)是广泛流行于世界各地的,危害人类身体健康,影响畜牧业发展的人兽共患原虫病[1-4]。国内外许多学者曾对人工感染小白鼠弓形虫病的途径、剂量、病状、病变进行大量研究[5-7]。为了对人工感染弓形虫病的小白鼠进行治疗药物的筛选,确定弓形虫病的适宜感染途径和剂量,掌握用药时机,拟定药效观察方法和指标,制订药效判定标准,家畜弓形虫病防治方法的研究课题组进     

猪弓形虫病诊断方法的研究进展

弓形虫病是一种人兽共患疾病,广泛分布于世界各地,对人及家畜的危害非常严重.人的先天性弓形虫病在胎儿或婴幼儿可出现发育畸形、智力障碍、脑炎、脑膜炎甚至死亡等临床症状;许多畜禽如猪、牛、猫、犬、羊、马、骆驼、家兔、鸡、鸭等都可以感染弓形虫且出现病症,其中以猪的感染率较高,该病曾给养猪生产造成巨大经济损失.为此,主要就猪弓形...     

动物弓形虫检测方法研究进展

弓形虫病(Toxoplasmosis)是由弓形虫(Toxoplasma gondii)引起的一种人兽共患寄生虫病,危害严重且分布广泛,目前尚未有理想的防治药物。因此,及时准确的检测对于防治弓形虫病尤为重要。就弓形虫的病原学检测、血清学检测及分子生物学检测等方法进行了综述,介绍了动物弓形虫检测中各类方法的研究现状及应用情况,分析了各种技术的优点与局限性,以期为动物弓形虫检测方法的发展和研究提供参考。     

弓形虫病免疫学诊断方法研究进展

弓形虫病是一种分布广泛、危害严重的人兽共患病,不但在公共卫生上有很重要的意义,而且对畜牧业也造成巨大经济损失.为了寻找敏感、特异、快速的弓形虫病诊断方法,国内外学者在弓形虫病诊断方面进行了大量研究.目前,弓形虫病调查、诊断的方法有病原学方法、免疫学方法、分子生物学方法,但免疫学方法仍是进行弓形虫感染调查、弓形虫病诊断的常用方法.检测抗弓形虫抗体或循环抗原的免疫学诊断方法主要有染色试验(DT)、凝集试验(AT)、酶联免疫吸附试验(ELISA)、免疫胶体金技术等.文章就该病的免疫学诊断方法的研究进展加以概述.     

一起猪弓形虫病的诊治

猪弓形虫病(Toxoplasmosis)是由刚第弓形虫引起的一种原虫病,又称弓形体病。弓形虫病是一种人畜共患病,宿主的种类十分广泛,人和动物的感染率都很高。据国外报道,人群的平均感染率为25%~50%,有人推算全世界约有至少5亿人感染弓形虫。猪暴发弓形虫病时可使整个猪场的猪只发病,死亡率高达60%以上。我国弓形虫感染和弓形虫病的分布     

Attempted transmission of Toxoplasma gondii infection from pregnant cats to their kittens

Sixteen 1- to 7-week-old pregnant specific-pathogen free cats were inoculated orally with Toxoplasma gondii cysts. Fetuses and neonatal kittens were examined for toxoplasma infection by inoculating suspensions of their tissues into mice. Toxoplasma gondii was not isolated from 23 fetuses and 16 newborn kittens from 13 queens. Six (3 litters) of the 15 kittens from the 3 remaining queens were killed on the day of or a day after birth, and the remaining 9 kittens were housed with their mothers for 7 to 33 days. None of the 9 kittens from the 2 litters examined between 0 and 33 days of age was infected with T gondii. In the other litter, T gondii was isolated from 3 kittens killed at 9, 16, and 22 days of age but not from 3 littermates killed on days 1, 1, and 22. Internal organs from the 3 kittens with proved toxoplasma infectivity in mice were examined histologically. Multifocal granulomatous encephalitis, hepatitis, nephritis, myocarditis, myositis, and interstitial pneumonia were found in all 3 kittens. Toxoplasma forms were demonstrated histologically in the tissues of 2 of these kittens. The mode of toxoplasma infection in newborn kittens was not determined but did not appear to be either transplacental or via fecal contamination from oocysts excreted by the mother cat. Evidence obtained in these experiments suggests that transplacental toxoplasma infection in the cat is not an important epidemiologic factor in perpetuation of the disease in the feline population.     

Intracranial Toxoplasma gondii granuloma in a cat

An 8-year-old cat with recent-onset generalized seizures was diagnosed with a right forebrain mass using magnetic resonance imaging. The mass was excised and upon histologic and immunohistochemical examination shown to be a Toxoplasma gondii granuloma. Serology supported active T gondii infection. The cat was treated with phenobarbital to control seizures. After definitive diagnosis of toxoplasma granuloma, clindamycin was administered for approximately 1 month. Seizures recurred 8 months after initial presentation, and the cat was euthanased at the owner's request. This is a previously unreported manifestation of feline central nervous system toxoplasmosis. When a mass lesion is present in the brain of a cat and serologic test results support active infection with T gondii, toxoplasma granuloma must be a differential diagnosis. If the patient is suffering from clinical disease, surgical resection of the mass (if possible) can be complimented with medical treatment until definitive diagnosis is obtained. Immunocompromising factors should be identified and addressed if possible.     

猪弓形虫病诊断与防治

猪弓形虫病是由刚地弓形虫感染引发的一种血液原虫病,该种疾病属于人畜共患病和典型的免疫性疾病,可以在多种牲畜群体中寄生。猪群一旦受到弓形虫感染,病情会快速传播,蔓延防控不及时,还会造成整个猪群全部发病,死亡率有时高达60%以上,是危害生猪养殖产业的常发病和高发病。因此,需要加强流行病学调查,在疾病进入流行高发期前,采取针对性措施防控,提高防控效果,降低发病率,保障养殖安全。该文探讨猪弓形虫病的流行病学、临床症状、病理变化、诊断方法和防治措施。     

龙岩市部分地区牛、羊弓形虫病的血清学调查

目的：采集龙岩市部分地区牛、羊血清,检测福建省牛和羊弓形虫病的感染情况。方法：应用酶联免疫吸附试验检测牛和羊血清弓形虫IgG抗体。结果：49份牛血清弓形虫IgG抗体均呈阴性;35份羊血清中,8份弓形虫IgG抗体呈阳性,阳性率为22．86％（8／35）。结论：龙岩市部分地区羊弓形虫的感染率较高,应引起人们重视。     

桦褐孔菌粗多糖对急性感染弓形虫小鼠的存活时间及SOD的影响

为探讨桦褐孔菌粗多糖(IOCP)对急性感染弓形虫小鼠的存活时间及血清、心、肝和肺中超氧化物歧化酶(SOD)含量的影响。采用昆明系小鼠80只,随机分为IOCP组、阳性对照组(蒿甲醚)、空白对照组和阴性对照组,每组20只。用RH株弓形虫建立小鼠病理模型,观察小鼠治疗后的存活时间,以及血清、心、肝和肺中SOD含量。结果:IOCP及阳性药物均可延长感染弓形虫小鼠的存活时间;可明显提高小鼠脏器SOD活性;与阳性对照组比较差异显著(P<0.05)。结论:IOCP能延长感染弓形虫小鼠的存活时间,并具有抗氧化损伤作用。     

Titration of Toxoplasma gondii oocysts in non-pregnant sheep and the effects of subsequent challenge during pregnancy

Fifty-nine ewes, seronegative to Toxoplasma gondii, were allocated to four groups which received 2000, 200, 20 or no M1 strain toxoplasma oocysts 56 days before mating. Fifty-one of them subsequently became pregnant and were challenged with 10,000 oocysts between 78 and 83 days of gestation. Infection with 2000 oocysts induced a pyrexia, seroconversion and protective immunity in all the recipient animals. Ewes that received either 20 or no oocysts before pregnancy were susceptible to subsequent challenge and severe fetal mortality occurred. In this study 200 oocysts was the threshold value for the induction of toxoplasma infection in sheep, although not all the ewes that seroconverted to this dose were protected against further challenge.     

弓形虫单克隆抗体的制备及胶体金免疫层析试纸条研制

弓形虫病(Toxoplasmosis)是由刚地弓形虫(Toxoplasma gondii,TOX)引起的一种重要人兽共患寄生虫病,该虫是一种可寄生于有核细胞的原虫。当前对弓形虫病的诊断和治疗虽已有一些方法,但均不够理想。本文就该病的免疫学诊断方法的研究进展加以概述,以研究SAG2为检测抗原,以制备弓形虫SAG2蛋白的单克隆抗体和免疫胶体金试纸条为核心,建立一种快速、特异、便捷的检测方法,为我国弓形虫病的诊断与防治、弓形虫与宿主关系、以及弓形虫的分子生物学等研究奠定良好基础。     

IFAT detection of IgG specific to toxoplasma in thoracic fluids from aborted lambs: evaluation on routine diagnostic submissions

Thoracic fluids from 171 aborted lambs from 55 flocks were examined by the indirect fluorescent antibody test for the presence of IgG specific to Toxoplasma gondii. The technique was shown to be both sensitive and specific for the diagnosis of toxoplasma abortion at a titre of 1/256, when compared with diagnoses made in the flocks and in the small number of individual fetuses in which the pathology of the cotyledons and the serology of the ewe were known.     

兰州地区宠物犬弓形虫病血清学检测分析

为了解兰州地区宠物犬弓形虫病的感染情况以及不同弓形虫检测方法检测结果的差异,本研究对兰州地区272例宠物诊所受检犬只进行弓形虫阳性检出率的统计分析,对66份犬血样进行间接血凝试验(IHA)与酶联免疫吸附试验(ELISA)检测,并将2种方法结果进行比较。结果表明弓形虫的感染率与年龄有关,随着年龄的增长感染率增加,犬只小于1岁感染率最低3.77%,1～3岁感染率为10.59%,3～5岁感染率为15.28%,大于5岁时感染率最高22.58%,不同性别之间感染无显著差异(P>0.05);IHA与ELISA检测结果经t检验比较差异不显著(P>0.05),2种检测方法中,IHA方法适用于大规模流行病学调查。