用酶联免疫吸附和正向间接血凝试验检测猪温抗体

应用ELISA和IHA对我省两种猪场的66份血清进行了检测，结果表明用ELISA检测两猪场免疫合格率分别为83．33％、80．5％，用IHA检测分别为90％、97．2％；两者检测结果符合率为84．8％，具有较高的群体相关性。     

ELISA和IHA检测弓形虫抗体的比较试验研究

目的:分析ELISA方法和IHA方法检测弓形虫病的可靠性。方法:本文应用酶联免疫吸附试验(ELISA)和间接血凝试验(IHA)两种方法,平行检测来自龙岩市某个猪场的32份猪血清中的抗弓形虫特异性抗体。结果:ELISA和IHA对猪弓形虫抗体阳性检出率分别为62.5%(20/32)、53.13%(17/32),这两种方法的阳性检出符合率较高,为71.88%(23/32)。其中,ELISA方法的敏感性(93.33%)、检测效率(81.25%)以及Youden指数(63.92%)都在IHA方法之上,但ELISA方法的特异性(12/17,70.59%)略低于I-HA方法(13/17,76.47%)。结论:ELISA和IHA两种方法均可用于猪弓形虫病的诊断和血清学调查,但ELISA方法更适用于猪弓形虫病的血清学调查。     

羊流产嗜衣原体病和弓形虫病IHA与ELISA诊断方法的比较

通过比较研究羊流产嗜衣原体与弓形虫抗体的间接血凝试验(IHA)与酶联免疫吸附试验(ELISA)检测结果,选择适宜贵州省山羊流产血清学调查的检测方法。通过IHA和ELISA两种方法对贵州省195份山羊血清进行羊流产嗜衣原体与弓形虫抗体的检测,统计并分析两种疫病的阳性符合率、阴性符合率和总符合率。结果表明,羊流产嗜衣原体与弓形虫IHA与ELISA的总符合率分别为77.95%和78.97%,阳性符合率均仅为50%,阴性符合率为80.45%和79.27%。说明在检测两种疫病血清抗体方面,IHA比ELISA更适合在贵州省基层推广。     

某猪场猪瘟免疫程序的调整及免疫效果评价

为了更好地预防和控制猪瘟,找出适合四川某猪场的猪瘟免疫程序,笔者根据实际情况对免疫程序进行了调整。然后用ELISA和IHA法对免疫程序调整前后的不同日龄猪血清进行了抗体检测,结果显示:调整前用ELISA法检测到3日龄、25日龄、50日龄、90日龄和120日龄猪只的猪瘟抗体阳性率分别为80%、54.54%、77.78%、80%和95%,用IHA法测得的阳性率分别为100%、63.64%、77.78%、90%和95%;调整后用ELISA法测得的猪瘟抗体阳性率分别为100%、80%、77.27%、77.78%和95%,用正向IHA法测得的抗体阳性率分别为97.73%、94.44%、96.15%、100%和100%。调整前用ELISA和IHA测得的平均阳性率为77.46%、85.28%,调整后则变为86.01%、97.66%,虽然均符合农业部颁布的猪群猪瘟抗体阳性率应不低于70%的标准,但免疫程序调整后的抗体阳性率明显高于调整前,可见调整后的免疫程序更适合于该猪场。     

3种猪瘟抗体检测试剂盒的比较与分析

为掌握3种猪瘟病毒抗体检测试剂盒的优缺点,本研究应用IHA、间接ELISA、阻断ELISA试剂盒分别对67份猪血清样品进行检测,结果显示IHA、间接ELISA、阻断ELISA分别检测出阳性样品48份、56份、59份,阳性率分别为71.64%、83.58%、88.06%,与中和试验的符合率分别为89.55%、92.53%、94.03%。结果表明IHA灵敏度稍低,对血清质量要求高,但检测快速、操作简单及无需特殊仪器设备,适合个体检测和基层实验室使用;间接ELISA和阻断ELISA敏感性高、通量高,但对操作人员有专业水平要求、需要酶标仪,适合对畜群检测和一般实验室使用。     

IHA法和间接ELISA法检测猪附红细胞体抗体结果对比分析

为了寻求适用于基层实验室对猪附红细胞体抗体的检测方法,文章试验用间接血凝试验(IHA)和间接ELISA两种方法分别检测了402份临床送检猪血清,并对这两种方法的敏感性和符合率进行比较研究。结果表明,IHA和ELISA法结果符合率为80.1%,且ELISA方法比IHA敏感性更高,更适合批量检测和群体普查。     

应用三种方法筛选猪瘟抗体阴性猪的比较试验

为了寻找筛选猪瘟抗体阴性猪更简便的方法,本试验应用猪瘟中和试验(SN)、阻断ELISA和正向间接血凝(IHA)三种方法分别对170份未经猪瘟活疫苗免疫的仔猪血清进行猪瘟抗体检测。结果显示,SN、ELISA和IHA检测出的猪瘟阴性血清分别为144份、150份和131份,表明三种检测方法有较好的符合率,其中SN与ELISA的检测符合率为90.59%,SN与IHA的检测符合率为82.94%,而ELISA法与另两种方法比较阴性符合率最高。本结果表明,ELISA法具有较高的敏感性,适合应用于猪瘟活疫苗安全检验抗体阴性猪的筛选。     

衣原体单克隆抗体在规模化牛羊场相关抗体的检测中的应用

采用淋巴细胞杂交瘤技术,研制出的抗衣原体单克隆抗体(McAb),应用酶联免疫吸附试验(ELISA)方法,对本地区所采的1361份牛血清,698份羊血清进行检测,结果:牛血清阳性检出率为9.18%,羊为22.06%。选择牛羊血清各50份用已建立的检测衣原体抗体ELISA方法与传统的间接血凝试验(IHA)进行比较,其敏感性高。牛血清ELISA阳性检出率为32%,IHA为24%,ELISA比IHA试验高出8个百分点。羊血清ELISA阳性检出率为26%,IHA为22%,前者比IHA高出4个百分点。     

某猪场猪口蹄疫免疫程序的调整及免疫效果评价

为探讨一种适合四川某猪场的猪口蹄疫免疫程序,笔者根据猪场的实际情况对原有免疫程序进行了调整,然后采集调整前和调整后不同日龄的猪血清,用ELISA和IHA进行抗体检测。结果显示:调整前3日龄、25日龄、50日龄、90日龄和120日龄猪的ELISA抗体阳性率分别为50%、30%、40%、50%、55%,IHA抗体阳性率分别为70%、54.54%、66.67%、70%和85%;调整后ELISA抗体阳性率分别为90%、35%、45.45%、61.11%和50%,IHA抗体阳性率分别为82.98%、83.33%、92.86%、95%和95%。可见调整后的免疫效果明显优于调整前,调整后的免疫程序为:25日龄首免,50日龄二免,90日龄三免,以后每3个月免疫一次。     

副猪嗜血杆菌血清5型间接血凝试验和间接ELISA抗体检测方法的建立

为建立副猪嗜血杆菌（HPS）的血清学诊断方法，通过探索HPS荚膜多糖产生的最适体外培养条件，提取了HPS血清5型菌株的荚膜多糖（CPS），并以之为抗原分别建立了间接血凝试验（IHA）和间接ELISA两种抗体检测方法，对其特异性、敏感性和符合率进行了比较研究。结果表明，两种检测方法的特异性良好，但ELISA的敏感性是IHA的5～10倍，二者的阳性符合率、阴性符合率和总符合率分别为79．7％、55．2％和65．3％。用这两种方法检测了320份临床送检猪血清，IHA和ELISA的阳性率分别为40％和59％。结果证实，这两种方法适用于不同实验室条件下HPS的诊断和流行病学调查。     

Development and evaluation of an enzyme-linked immunosorbent assay for bovine antibody (IgG) to Pasteurella haemolytica

The sensitivity of an indirect enzyme-linked immunosorbent assay (ELISA) for bovine IgG serum antibody to Pasteurella haemolytica was compared with that of an indirect hemagglutination (IHA) test. Pasteurella haemolytica serotypes were grown in a chemically defined cell culture medium, and soluble antigens released into the growth medium were used in the ELISA and IHA test. An ELISA with serotype-1 antigen consistently detected antibody in sera that were positive by IHA test (correlation, 99%). Sera reacting with serotype-1 ELISA antigens also reacted with ELISA antigens prepared from other serotypes. Although ELISA titers averaged 5 log2 units higher than IHA titers, plots of titers determined by the 2 methods were approximately linear. Titer increases detected in paired serum samples by either test were similar. The ELISA was more sensitive than was the IHA in detecting colostral IgG antibody in serum of newborn calves. The ELISA uses a simple, stable antigen preparation and detects antibody to P haemolytica serotypes that commonly infect cattle.     

Evaluation of a monoclonal blocking ELISA and IHA for antibodies to Mycoplasma hyopneumoniae in SPF-pig herds.

A monoclonal blocking enzyme-linked immunosorbent assay (ELISA) and an indirect haemagglutination assay (IHA) were applied to serum samples from 124 specific pathogen-free (SPF) breeding and multiplying herds, which participate in the routine serological surveillance of the Danish SPF programme. Clinical and pathological observations of the herds and microbiological culturing of Mycoplasma hyopneumoniae were used to calculate herd sensitivity, herd specificity and herd predictive values for the two serological assays. The ELISA was superior to the IHA in herd sensitivity and herd specificity, with values of 93 per cent and 96 per cent, respectively, for the ELISA, and 61 per cent and 92 per cent for the IHA. During the six month period of evaluation 2.5 per cent of the herds were infected with M hyopneumoniae each month. At this level the IHA was found to have a positive herd predictive value of 16 per cent, compared with 39 per cent for the ELISA. The negative herd-predictive value on the same level was 99.8 per cent for the ELISA and 98.9 per cent for the IHA. If the assays were applied to a group of herds with a herd prevalence of M hyopneumoniae infection of 30 per cent (as is the case with the production herds in the Danish SPF programme) the predictive value of a positive herd diagnosis would be 91 per cent for the ELISA and 76 per cent for the IHA, and the predictive value of a negative herd diagnosis would be 97 per cent with the ELISA and 85 per cent with the IHA.(ABSTRACT TRUNCATED AT 250 WORDS)     

贵州省猪弓形虫病的血清学调查

为全面了解贵州省猪弓形虫感染情况,对采自9个市（州、地）264个养猪场（户）的2 906份血清用酶联免疫吸附试验（ELISA）检测猪弓形虫抗体,总体阳性率为65.83%,变化范围为27.88%~85.42%。采集65份猪血清做ELISA和间接血凝试验（indirect heamagglutination assay,IHA）比较测定,两种方法总体符合率为58.46%。IHA方法补充检测177份猪血清,弓形虫抗体阳性率为27.68%。血清学调查结果与国内部分省（市）报道相符。     

Comparison of enzyme-linked immunosorbent assay and indirect hemagglutination test for quantitating antibody responses in chickens against Pasteurella multocida

An indirect enzyme-linked immunosorbent assay (ELISA) was developed to measure humoral antibody responses of chickens against Pasteurella multocida. A standard indirect hemagglutination (IHA) test was used to compare serologic results with those of ELISA. The ELISA was also used following challenge with P. multocida to compare the efficacy of three commercial fowl cholera vaccination regimens. Although antibody titers measured by ELISA and IHA were highly correlated, ELISA was at least twice as sensitive as IHA. Antibody measured by ELISA and IHA also correlated significantly with protection against P. multocida challenge. No mortality occurred in any of the three vaccinated challenged groups. However, control unvaccinated chickens experimentally infected with P. multocida developed signs of acute pasteurellosis and died by the 10th day post-challenge. Impression smears made of hepatic tissue from all chickens were stained (Wright's stain), and typical bipolar rods characteristic of Pasteurella were identified in smears from unvaccinated challenged controls only.     

基于新城疫病毒V蛋白鉴别鸡群感染与免疫的间接ELISA方法的建立

本研究以新城疫病毒(NDV)V蛋白羧基端结构域(Vc)的重组蛋白为包被抗原,建立了用于检测NDV V蛋白抗体的间接ELISA方法,并采用该方法检测了鸡群免疫或接毒后血清中的V蛋白抗体水平。结果显示:两组不同NDV灭活疫苗组在免疫后的3周内检测结果均为阴性;两组灭活疫苗免疫3周后再人工感染NDV强毒的鸡群,攻毒后第7、14和21 d,NDV阳性率分别为60%、80%、70%和50%、80%、70%;两组不同的NDV弱毒疫苗免疫组鸡群,仅在免疫后第21 d阳性率分别为20%和10%。以上结果表明,NDV疫苗免疫组与强毒感染组的V蛋白抗体阳性率存在明显差异,本方法可在群体水平上区分新城疫疫苗免疫与强毒感染鸡群,为NDV血清学诊断和流行病学调查提供了一种新的检测手段。     

Evaluation of an enzyme-linked immunosorbent assay for detection of Eperythrozoon suis antibodies in swine.

An ELISA was developed and tested to detect antibodies to Eperythrozoon suis in swine. Results were compared with those of the indirect hemagglutination (IHA) test. Antigen isolated from swine heavily infected with E suis was used for both tests. Comparison of the ELISA with the IHA test revealed a significant (P less than 0.001) correlation between results. Of 114 samples obtained from 9 swine infected with E suis, 87.7% were seropositive (titer greater than or equal to 200) via the ELISA, and 80.7% were seropositive (titer greater than or equal to 20) via the IHA test. The sensitivity of the ELISA was greater than that of the IHA test. All blood samples obtained from specific-pathogen-free swine tested negative for E suis antibody. Cross-reactions were not observed between E suis antigen and antisera against various swine and cattle disease agents using ELISA. We concluded that the ELISA may be used for rapid and effective diagnosis of infection with E suis in swine.     

A monoclonal blocking ELISA detecting serum antibodies to Mycoplasma hyopneumoniae.

A monoclonal blocking enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Mycoplasma hyopneumoniae in porcine serum has been developed. The monoclonal antibody (mAb) reacts with an M. hyopneumoniae specific epitope on a molecule of approximately 74 kDa. Only sera from M. hyopneumoniae infected pigs were able to block the binding of the mAb although antibodies from M. flocculare infected pigs also recognized a 74 kDa molecule. Sera from experimentally infected pigs as well as field samples were compared by the ELISA and by an indirect hemagglutination assay (IHA). In experimental pigs, the earliest detectable antibody response was found to be almost identical for both assays, but for some of the pigs the time of detection was significantly earlier by blocking ELISA than by IHA. In naturally infected herds more samples were found to be positive by ELISA than by IHA. Furthermore, the results indicate that sera from naturally M. flocculare infected pigs may give rise to cross-reactions in the IHA. The blocking ELISA appears to be a valuable and reproducible tool in the surveillance and serodiagnosis of M. hyopneumoniae infections in pigs.