东北林蛙消化系统LDH同工酶表型的季节变化

为了解小兴安岭地区不同季节东北林蛙消化系统中食道、胃、肠道和肝脏的乳酸脱氢酶(LDH)同工酶的分布情况和活性,试验采用聚丙烯酰胺凝胶垂直板电泳对其进行分离分析。结果表明:东北林蛙消化系统4种器官组织中LDH同工酶共分离出电泳迁移率为0.299~0.153,基因型为C’4、CC’3、C2C’2、C3C’、C4、AB2C、B4、B’4、A2C2、A2B2、A2B’2、A3B、A4的LDH1~LDH13的13条谱带。4种器官组织在不同季节分离出的谱带数不同,在春季食道、胃、肠道和肝脏中均分离出11条谱带,夏季分别分离出10,10,11,9条谱带,秋季分别分离出4,4,5,5条谱带,冬季分别分离出7,6,6,6条谱带。电泳迁移率为0.255,0.242的LDH6和LDH7为冬季特有谱带。各组织中LDH13活性均最强。LDH同工酶的分布和活性具有明显的组织特异性,在不同季节的各组织器官中的表达程度不同,呈现多态性,具有季节性的差异。     

东方田鼠不同器官组织同工酶研究

为了对东方田鼠的深入研究及鼠害防治提供基础的生理生化指标依据,试验采用聚丙烯酰胺凝胶垂直板电泳的方法对东北林区东方田鼠的心脏、肝脏、肾脏、骨骼肌、脑、睾丸6种器官组织中的乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)同工酶进行分离、分析。结果表明:东方田鼠心脏、肾脏、骨骼肌、脑、睾丸5种器官组织中均有LDH1~LDH5的5条谱带出现,而在肝脏中只出现1条谱带为LDH5,在心脏、肾脏、骨骼肌、睾丸4种器官组织中均有亚带出现,6种器官组织中的LDH同工酶活性不同;在东方田鼠心脏、肝脏、肾脏、骨骼肌、脑、睾丸6种器官组织中的SOD同工酶中共分离出SOD1~SOD18的18条谱带,其中SOD4、SOD9、SOD14、SOD154条谱带为6种器官组织中共有谱带,且SOD15谱带在6种器官组织中活性最强。2种同工酶在6种器官组织中的谱带分布及酶活性存在明显的组织特异性。     

进化水平不同的动物和人血清乳酸脱氢酶同工酶电泳图谱的比较研究

应用聚丙烯酸胺垂直板梯度凝胶电泳分析了进化水平不同的17种动物和人血清乳酸脱氢酶(LDH)同工酶.17种动物是鲤鱼、中华大蟾蜍、蝮蛇、罗斯蛋鸡、山鸡、紫貂、银狐、貉、梅花鹿、马鹿、黑白花奶牛、新疆细毛绵羊、猪、马、大耳白兔、昆明小鼠和猴.在同一凝胶板、同一条件下,对这些动物和人血清LDH同工酶谱分析表明,LDH同工酶谱在各类动物之间存在明显差异.进化比较低级的动物(杂交鲤鱼除外),血清LDH同工酶谱带迁移率较小,接近阴极;反之,进化比较高级的动物,同工酶谱带迁移率较大,接近阳极.亲缘关系相近的动物,LDH同工酶谱相似.表明LDH同工酶谱带的差异与动物间的亲缘关系有关.同种不同个体的动物LDH同工酶谱差异不明显,只有杂交鲤鱼个体LDH同工酶谱存在明显差异.     

燕雀6种器官组织中SOD和LDH同工酶电泳分析

为了给鸟类的深入研究提供基础的生理生化指标依据,试验采用聚丙烯酰胺凝胶垂直板电泳的方法对燕雀心脏、肝脏、肾脏、肌肉、脑、肺脏6种器官组织中乳酸脱氢酶(LDH)和超氧化物歧化酶(SOD)同工酶进行分离、分析。结果表明:燕雀6种器官组织中共分离出SOD1~SOD16、电泳迁移率为0.800~0.113的16条谱带,6种器官组织中共分离出LDH1~LDH5、电泳迁移率为0.152~0.097的5条谱带。LDH和SOD同工酶在不同组织中有谱带缺失现象,同一个体在同一组织内和不同组织之间同工酶谱带分布和酶活性存在明显差异。     

山西黑猪不同器官组织乳酸脱氢酶同工酶活性分析

为了探究哺乳动物中乳酸脱氢酶(LDH)同工酶的种类及不同器官组织中LDH同工酶的活性及相对含量,试验以山西黑猪为试验材料,利用紫外分光光度计和聚丙烯酰胺凝胶电泳对不同器官组织中LDH活性和同工酶谱带进行检测和分离。结果表明:不同器官组织中LDH的活性存在明显区别,不同器官组织中所含LDH同工酶的种类和相对含量各不相同,不同器官组织中相同LDH同工酶的相对迁移率差异不大。说明动物机体中同工酶的保守性较高。     

棕黑锦蛇雌雄个体不同器官/组织同工酶比较分析

为了研究棕黑锦蛇雌雄个体不同器官/组织同工酶,试验采用聚丙烯酰胺凝胶垂直板电泳方法对棕黑锦蛇心脏、肝脏、肾脏、肌肉、大脑、肺脏、睾丸7种器官/组织(雌性6种)中乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、酯酶(EST)进行分离、分析。结果表明:棕黑锦蛇不同组织中共分离出LDH1~LDH5、电泳迁移率为0.129~0.291的5条谱带,共分离出SOD1~SOD13、电泳迁移率为0.093~0.954的13条谱带,共分离出EST1~EST9、电泳迁移率为0.121~0.595的9条谱带。各种器官/组织中,心脏中LDH同工酶活力最强,肝脏中SOD同工酶活力最强,肾脏中EST同工酶活力最强。棕黑锦蛇雌雄个体之间及同一个体、不同器官/组织之间同工酶谱带分布和酶的活性存在明显差异。     

叶尔羌高原鳅三种同工酶的组织差异性研究

为了探究叶尔羌高原鳅同工酶表达的组织差异性和基因位点表达特征,试验采用不连续聚丙烯酰胺凝胶电泳技术对养殖和野生个体的心脏、肌肉、肝脏和肾脏4种组织器官的乳酸脱氢酶(LDH)、酯酶(EST)和苹果酸脱氢酶(MDH)进行分离、分析。结果表明:叶尔羌高原鳅LDH由2个基因位点编码,4种组织器官中共检测到3条酶带,组织差异性不明显;EST由3个基因位点编码,4种组织器官中共检测到7条酶带,其中EST-1a和EST-3b为各组织器官共有酶带,具有明显的组织差异性;MDH的S-MDH和M-MDH型同工酶在各组织器官中均有表达,4种组织器官中共检测到5条酶带,但酶带分布具有明显的组织差异性。根据酶谱特征对其种质鉴定进行了分析发现,叶尔羌高原鳅同工酶具有自属的一套同工酶系统特征。     

牦牛睾丸附睾LDH同工酶表达模式的研究

利用比色法和聚丙烯酰胺凝胶电泳(PAGE)技术对6、12、18、24月龄半血野牦牛、横交半血野牦牛和家牦牛睾丸、附睾组织LDH同工酶活性及谱带表达进行测析.结果表明睾九、附睾LDH在相同月龄其表达模式不同,6月龄睾丸LDH有4条酶谱,活性百分率依次(LDH1＞LDH3＞LDH2＞LDH4),附睾只有3条酶谱(LDH1＞LDH2＞LDH3);到12、18月龄时,睾丸LDH有5条酶谱(LDH1＞LDH3＞LDH2＞LDH2＞LDH4＞LDH5),附睾4条酶漕(LDH1＞LDH2＞LDH3＞LDH4),睾丸和附睾LDH同工酶随月龄增长和睾丸组织发育,活性和酶谱表现出明显组织器官的特异性.3种类型牦牛LDH同工酶表达基本相似.24月龄时,睾丸、附睾LDH有6条谱带,LDH-X位于LDH4与LDH5之间,证明此时牦公牛睾丸中精子成熟.     

棕黑锦蛇消化系统同工酶电泳分析

为给棕黑锦蛇人工养殖和开发利用等提供生理生化指标依据,试验采用聚丙烯酰胺凝胶垂直板电泳方法,对棕黑锦蛇消化系统中食道、胃、小肠、大肠、肝脏、胰腺6种器官中乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)同工酶进行分离分析。结果表明:6种器官中共分离出LDH1~LDH5、电泳迁移率(Rm)0.259~0.156的5条谱带,LDH1~LDH3、LDH54条谱带为6种器官中的共有谱带,Rm为0.184的LDH4为胃中的特有谱带;6种器官中共分离出SOD_1~SOD_(23),Rm为0.868~0.033的23条谱带,食道、胃、小肠、大肠、肝脏、胰腺中分别分离出9条、13条、13条、8条、14条、15条谱带;SOD_5,SOD_6,SOD_(11),SOD_(12)4条谱带为6种器官共有,各器官间和器官内LDH、SOD同工酶的谱带分布和活性均有差异。     

黑白花奶牛血清乳酸脱氢酶活性及其同工酶的测定

目前,国内外关于各品种牛的乳酸脱氢酶(LDH)同工酶的研究,曾有不少报道。LDH同工酶在临床上对受损器官和组织的定位、疾病的性质及预后判断具有重要意义。而且,不同种类的动物LDH同工酶的相对活力有明显的种间差异,故可作为鉴别种的参考。为适应临床诊断和遗传学研究的需要,我们测定了新疆石河子地区的黑白花奶牛LDH活性正常值及其同工酶酶谱。     

Heartworm in dogs in Canada in 1984

In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.

Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.

     

Heartworm in dogs in Canada in 1985

In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).

Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1988

In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%).     

Heartworm in dogs in Canada in 1989

In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.

Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.

     

Heartworm in dogs in Canada in 1987

In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%).     

Heartworm in dogs in Canada in 1986

In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).

Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1983

In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.

Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.