蜂群衰竭症威胁美国农业

2006年美国大陆有惊人数量的蜜蜂群死亡.随后的调查推测,这种不明原因的蜂群消失至少在最近两年是养蜂人经历过的.到2007年春季全美国有20多个州报道了蜜蜂灾难性的损失.蜜蜂飞出去采集花粉和花蜜,却神秘地不再返回蜂巢,目前还查不出原因.这种情况经过媒体的报道,特别是在万维网上迅速的传播(到2007年7月上旬"谷歌"网上已有621 000项关于蜜蜂神秘失踪的报道),引起了世界各国的科学家、养蜂企业和农场主的关怀.     

鹅鸭瘟病的诊断与防治

鸭瘟本是鸭的一种急性败血性传染病,在同病鸭密切接触的情况下,鹅也会感染发病,鹅的鸭瘟病以流泪、头颈肿大、泄殖腔溃烂为主要特征. (一)病原的特征 该病由鸭瘟病毒感染引起,存在于病鸭(鹅)的各个内脏器官、血液、分泌物、排泄物中.鸭瘟病毒不耐热,但对低温抵抗力较强,对乙醚和氯仿敏感,对常用的消毒药抵抗力不大.     

农业部"十五"重点推广的毛绒用羊良种

一、澳洲美利奴羊 澳洲美利奴羊产于澳大利亚,是由西班牙美利奴羊(英国、南非)、兰布列羊(法国、美国)等进行杂交选育而成的著名细毛羊品种.我国引入多年,并以此为素材之一培育出了中国美利奴羊.澳洲美利奴羊具有毛丛结构好、羊毛长而明显弯曲、油汗洁白、光泽好、净毛率高、毛密度大、细度均匀的特点,对各种环境气候有很强的适应性.体型近似长方形,腿短、体宽、背部平直,后躯肌肉丰满;公羊颈部由1～3个发育完全或不完全的横皱褶,母羊有发达的纵皱褶.羊毛覆盖头部至两眼连线,前肢达腕关节,后肢达飞节.     

什么犬适合用作"军犬"

能用作军犬的犬种并没有严格的标准,广义地来说,只要能被训练后用在军事事务如:追踪、鉴别、警戒、看守、巡逻、搜捕、通讯、携弹、侦破、搜查毒品、爆炸物等任务,都可被认可为军犬.特别是在战场上,只要能作战就算是军犬.……     

缉毒犬搜出贩毒物证

2007年1月31日上午,秦皇岛市公安局刑警支队技术三大队接到指令:缉毒部门抓获贩毒嫌疑人员尤某,在其临时住所——秦皇岛市海港区某住宅查获了冰毒近30克,麻古近200粒。但查获数量     

责任编辑

鱼市有"鱼霸",菜行有"菜霸",乡村中有"村霸".而专门欺侮养蜂人的称为"蜂霸".每个转地放蜂的蜂农都有被"蜂霸"盘剥、讹诈、欺骗的经历.造成的经济损失,少则数百元,多则数万元.例如吉林省露水河椴树场地某林场,当地有一伙人,对外省放蜂的蜂场收1 000元所谓押金(属巧立名目),待摇蜜时蜂蜜必须卖给他们.这伙人限制其他人收购蜂蜜,而他们压级压价强行收购蜂蜜,转手就可渔利,蜂农违心地卖蜜.如不卖给这伙人,事先交的1 000元"押金"就难以讨回.内蒙古牙克石油菜场地,也有强行收蜜,压级压价的"蜂霸".     

马鞍伤诊治一例

永康市龙川公园挽马1匹,体型高大。数月前,马背红肿,继而溃烂流脓。畜主多次用抗生素治疗无明显好转。经检查,马背左肩部大面积红肿,溃疡流脓,触痛明显,拒绝按压,体温略升。检查后发现马鞍偏小,属典型的大马配小鞍,因摩擦过度造成伤害,诊断为马鞍伤。治疗用大油剂青霉素2瓶(300     

一例金钱豹肠梗阻的治疗

2007年5月15日园主前来我医院咨询,主诉:昆明动物园有一金钱豹,猫科,雌性,体重60 kg,年龄15岁,毛色金钱带黄棕色,不食有2 d,且呕吐、不排便、精神沉郁,曾用一些抗生素治疗无效,我院随园主前往治疗,现将总体情况表述如下:     

蜂胶——肠胃炎的克星

巢脾是蜜蜂蜂巢的主要组成部分,其主要作用是繁殖幼蜂和贮藏蜂蜜、花粉.如不对以下异常巢脾及时妥当处理,则会严重影响蜂蜜的产量和质量,降低养蜂收益.     

猪冬季颈椎风湿症的治疗措施

2006年12月初北安市杨家乡某猪场有38头猪食欲减退,体温升高到39℃左右,常突然卧地不起,呈侧仰状,耳、鼻、四肢冰凉;眼结膜潮红,口唇皮肤呈青紫色,肌肉颤抖,触之尖叫;颈脊椎骨稍强直,前肢软弱、呈游泳状,后肢麻木无力。有2头母猪和12只仔猪因医治不及时死亡。1治疗措施后即可运动自如,食欲明显增强。之后,每天颈肌注射复方当归针(10毫升)2支、祖师麻(10毫升)2支,一次注射,连续3天好转。2预防在寒冷低温季节,多给猪铺垫稻草等保暖物,让猪多晒太阳,并适当通风换气,同时,加强营养,并在新鲜饲料中添加微量元素。(012)猪冬季颈椎风湿症的治疗措施…     

Antimicrobial resistance of Escherichia coli O26 and O111 isolates from cattle and their characteristics

The present study was to investigate antimicrobial resistance profiles of Escherichia coli O26 and O111 from cattle and to characterize the virulence genes of the resistant isolates. This paper reports the high prevalence of antimicrobial resistant E. coli O26 and O111 from cattle. Among 37 E. coli O26 and 25 E. coli O111 isolates from the fecal specimens obtained from cattle, 26 (70%) and 15 (60%) were resistant to at least one antibiotic, respectively. Forty (98%) of the 41 resistant isolates were resistant to two or more antibiotics. Among the 22 antibiotics tested in this study, ampicillin was the most common antibiotic that the isolates were resistant to, followed by tetracycline and streptomycin. None of the isolates were resistant to fluoroquinolones, such as ciprofloxacin, ofloxacin and norfloxacin, and to ceftriaxone, amikacin and imipenem. Eighteen different resistant types among the 41 isolates were observed by the cluster analysis. The most frequent antibiotic-resistance type was ampicillin-tetracycline-streptomycin-cephalothin-sulfisoxazole-ticarcillin-kanamycin-minocycline-piperacillin-chloramphenicol, which accounted for 9 (22%) of the resistant isolates. The observation of frequent and multiple resistances to antibiotics highlights the need for their careful use if their benefits are to be preserved. PCR analysis of the EHEC virulence markers showed that 25 of the resistant E. coli O26 and O111 isolates tested positive for stx2 or both stx1 and stx2. This suggests that the majority of these isolates can cause serious diseases in humans and may complicate the future therapeutic options under development.     

Antibiotic resistance among indicator bacteria isolated from healthy pigs in New Zealand

AIM: To determine the resistance to antibiotics among the indictor bacteria, Escherichia coli and Enterococcus spp, isolated from the faeces of healthy pigs on three conventional pig farms and one organic farm in the North Island of New Zealand. METHODS: Faecal samples, collected at intervals between March and October 2001, were plated onto MacConkey agar and Slanetz-Bartley agar and examined after 1-3 days incubation for colonies resembling E. coli and Enterococcus spp, respectively. Typical colonies were subcultured for further identification and storage. The isolates were tested for antibiotic resistance, using disc diffusion, to ampicillin, gentamicin, streptomycin, and tetracycline. Escherichia coli isolates were also tested for resistance to ciprofloxacin, cotrimoxazole and neomycin. Enterococcus spp isolates were also tested for resistance to vancomycin, erythromycin and virginiamycin. RESULTS: A total of 296 E. coli and 273 Enterococcus spp isolates were obtained from the three conventional farms, and 79 E. coli and 80 Enterococcus spp isolates were obtained from the organic farm. All the E. coli isolates from both the conventional and organic pig farms were susceptible to ciprofloxacin, and all the Enterococcus spp isolates were susceptible to ampicillin, gentamicin and vancomycin. Isolates of E. coli from conventional pig farms were resistant to gentamicin (0.7%), neomycin (0.7%), ampicillin (2.7%), cotrimoxazole (11%), streptomycin (25%) and tetracycline (60%). Enterococcus spp isolates from the same farms were resistant to erythromycin (68%), tetracycline (66%), streptomycin (54%) and virginiamycin (49%). By contrast, for the organic pig farm 相似文献   

新疆伊犁某牛场大肠杆菌耐药性调查

为了调查新疆伊犁某牛场饮水、饲料和粪样中分离的大肠杆菌对临床常用抗菌药物的耐药情况。本试验采用微量肉汤稀释法,对饮水源、饲料源、牛粪源样品中分离出的大肠杆菌进行最小抑菌浓度测定。结果显示,25份牛场饮水源样品,大肠杆菌分离率为100.0%（25/25）,对阿莫西林/克拉维酸（12.0%）、氨苄西林（4.0%）、诺氟沙星（4.0%）、恩诺沙星（8.0%）和安普霉素（8.0%）5种抗菌药物耐药;72份牛场饲料源样品,大肠杆菌分离率为65.3%（47/72）,对阿莫西林/克拉维酸（36.2%）、氨苄西林（19.1%）、诺氟沙星（4.3%）和安普霉素（4.3%）4种抗菌药物耐药;80份牛粪源样品,大肠杆菌分离率为100.0%（80/80）,对阿米卡星（12.5%）、氨苄西林（7.5%）、恩诺沙星（7.5%）、庆大霉素（5.0%）、诺氟沙星（2.5%）、环丙沙星（2.5%）、阿莫西林/克拉维酸（1.3%）和头孢噻呋（1.3%）8种抗菌药物耐药,仅对安普霉素敏感。该牛场分离的大肠杆菌对常用抗菌药物耐药情况一般,但中介率较高,须在临床治疗细菌性疾病中避免使用不敏感和中介率高的抗菌药物,养殖场饮水和饲料有被耐药大肠杆菌污染的风险。     

河北省犊牛腹泻大肠杆菌致病性及耐药性分析

为确定河北省犊牛腹泻大肠杆菌的致病性与耐药性,本研究对2018年1月至2019年6月期间从河北省石家庄、保定、承德、唐山、廊坊的部分肉牛场腹泻犊牛样品中分离到的50株大肠杆菌进行了致病性试验、药物敏感性试验、毒力基因和耐药基因检测。结果显示:50株分离菌均对小鼠具有致病性,致病菌占比100%(50/50)。毒力基因fyuA、irp2、eaeA、ler检出率分别为68.0%、66.0%、34.0%、34.0%。50株分离菌均对15种抗生素中的2种及以上表现为耐药,对10种及以上抗生素耐药的菌株占比达34%(17/50);分离菌对土霉素、磺胺对甲氧嘧啶、磺胺间甲氧嘧啶、替米考星4种药物表现为高度耐药,耐药率分别为90%、90%、94%、100%。除四环素类tetD基因检出率为0外,其它耐药基因均有检出,其中四环素类tetC、氨基糖苷类aadA1、喹诺酮类gyrA、gyrB基因检测率高达100%。试验的50株大肠杆菌均具有较强的毒力和多重耐药性。本研究为河北省大肠杆菌所致犊牛腹泻病的防治提供了实验依据。     

新疆部分地区奶牛乳房炎大肠杆菌的分离鉴定及耐药性分析

[目的]为了解本地区奶牛乳房炎大肠杆菌的流行及耐药情况。[方法]对新疆部分地区奶牛乳房炎大肠杆菌进行分离培养、染色镜检、荧光PCR鉴定,并用纸片法对分离菌株开展药物敏感试验。[结果]从200 份奶牛乳房炎样品中分离出63 株大肠杆菌,分离率为31.5%。分离菌株对青霉素耐药率最高,达到了93.65%,对美罗培南敏感率最高,达到了98.14%,对其他抗生素均有不同程度的耐药。[结论]新疆部分地区奶牛乳房炎中大肠杆菌广泛流行,具有较高的耐药性。     

内蒙古地区羊源大肠杆菌耐药性研究

为确定内蒙古地区羊源大肠杆菌的耐药表型及其耐药基因的流行情况,本研究采用微量稀释法测定了内蒙古地区108株羊源大肠杆菌对13种临床常用抗菌药物的最小抑菌浓度。结果显示,分离菌株对阿莫西林、头孢噻吩、磺胺甲唑、黏菌素的耐药率最高,均达100.0%,对阿莫西林/克拉维酸、四环素、环丙沙星的耐药率在50%~80%之间,对头孢噻肟、美洛培南、新霉素的耐药率均低于10%,较为敏感。108株羊源大肠杆菌中耐7种以上药物的菌株占94.4%,其中15.6%菌株对13种抗菌药物耐药,只有1株菌对所有抗菌药物敏感。采用PCR方法对羊源大肠杆菌分离株所携带的6种相关耐药基因进行检测,结果显示,6种耐药基因中的4种耐药基因bla_TEM、proP-2、sul-Ⅰ、ampG检出率超过50%,耐药基因aph（3'）-Ⅰ携带率达40%,只有耐药基因aac（3）-Ⅱ检出率仅为5.5%。由此可见,内蒙古地区羊源大肠杆菌对13种抗菌药物产生了不同程度的耐药性,且存在严重的多重耐药情况,羊源大肠杆菌分离株携带aph（3'）-Ⅰ、sul-Ⅰ、ampG、bla_TEM、proP-2、aac（3）-Ⅱ耐药基因。     

Study on the Drug-resistance of Escherichia coli Isolated from Sheep in Inner Mongolia

In order to determine the drug resistance phenotype and prevalence of drug resistance genes in Escherichia coli (E.coli) isolated from sheep in Inner Mongolia, the minimum inhibitory concentrations (MICs) of the isolates to antibiotics commonly used in veterinary clinical were detected by micro-dilution method in vitro. The results showed that the highest resistance rates of the isolates to amoxicillin,cephalothin,sulfamethoxazole and polymyxin were up to 100.0%,respectively.To amoxicillin-clavulanic, tetracycline and ciprofloxacin were 50% to 80%. These isolates were sensitive to cefotaxime, meropenem trihydrate, neomycin, and their resistance rates were all less than 10%. Among the 108 strains of E.coli from sheep, 94.4% of them were resistant to more than 7 antimicrobial agents,15.6% of them were resistant to 13 antimicrobial agents, only one strain was sensitive to all antimicrobial agents. Six kinds of resistance genes among the 108 E.coli isolates were detected by PCR method.The results showed that detection rates of 4 kinds of drug resistance genes including bla_TEM, proP-2, sul-Ⅰ and ampG were all over 50%,the detection rate of resistance gene aph (3')-Ⅰ was up to 40%, only resistance gene aac(3)-Ⅱ detection rate was 5.5%. Thus, the sheep E.coli isolates in Inner Mongolia produced various degrees of resistance to 13 kinds of antibiotics, and their multi-drug resistances were very serious. They carried aph(3')-Ⅰ, sul-Ⅰ, ampG, bla_TEM, proP-2 and aac(3)-Ⅱ resistance genes.     

新疆地区牛和新引进牛大肠埃希菌耐药性比较

为了解新疆地区牛和新引进牛大肠埃希菌对临床常用抗菌药物的耐药差异,采用微量肉汤稀释法对分离的大肠埃希菌进行耐药性检测,通过卡方检验比较新疆地区牛和引进牛大肠埃希菌耐药性的差异。结果显示,新疆地区牛大肠埃希菌和引进牛大肠埃希菌除对氨苄西林(地区牛为11.90%、引进牛为6.25%)和阿莫西林(地区牛为5.55%、引进牛为5.21%)有较高的耐药率外,对其他临床常用抗菌药物的耐药率均较低,但引进牛对氟苯尼考、庆大霉素和安普霉素的中度敏感率分别为82.81%、44.79%和33.33%。经卡方检验比较,新疆引进牛对氟苯尼考、庆大霉素、安普霉素、阿莫西林和氨苄西林的中度敏感率显著高于新疆地区牛(P0.05)。虽然被检牛源大肠埃希菌对常用抗菌药物耐药较低,但引进牛大肠埃希菌高中度敏感率会导致其耐药率短期内迅速增高,因此须在临床治疗引进牛细菌性疾病中避免使用中度敏感率高的抗菌药物。     

2019年湖南省部分地区鸡、鸭、猪源大肠杆菌耐药性调查

旨在调查2019年湖南省部分地区不同来源的大肠杆菌对抗生素的耐药水平,为养殖合理用药提供参考。从4个市畜禽养殖场采集猪、鸡和鸭粪便285份,使用麦康凯琼脂培养基和伊红美蓝培养基对大肠杆菌筛选,利用基质辅助激光解析电离飞行时间质谱进行鉴定,采用微量肉汤稀释法检测16种抗生素对大肠杆菌的最小抑菌浓度(MIC)。共分离出203株大肠杆菌(鸡源146株、鸭源20株和猪源37株),对四环素和氨苄西林耐药率最高,分别达到89.2%和84.7%;对头孢他啶、奥格门丁、黏菌素耐药率较低,分别为2.5%、2.0%和0.5%;所有菌株均对美罗培南敏感。抗3种及以上抗生素的菌株数目占比为87.7%,而仅5.42%的菌株对所有检测的抗生素敏感。另外,来源于减抗示范养殖场的大肠杆菌对大部分抗生素的耐药率,与来源于非减抗养殖场大肠杆菌相比未见显著差异。上述结果表明,湖南省动物源大肠杆菌的耐药水平仍处在较高水平,重视兽药使用管理并持续开展对大肠杆菌的耐药性监测十分必要。     

猪源耐黏菌素大肠杆菌PFGE分型及耐药性研究

为了解河南省焦作市、许昌市规模化养殖厂耐黏菌素大肠杆菌的流行情况,对分离到的32株耐黏菌素大肠杆菌使用微量肉汤稀释法检测其药物敏感性,并采用脉冲场凝胶电泳(Pulsed field gel electrophoresis,PFGE)对分离株进行分型研究。结果显示,32株耐黏菌素大肠杆菌对四环素、多西环素、磺胺异噁唑、复方新诺明的耐药率均高达90%以上,32株猪源大肠杆菌可分为30种带型。结果表明,两地市养殖场猪源大肠杆菌耐药性普遍存在,且以多重耐药为主,PFGE分型结果显示,32株耐黏菌素大肠杆菌整体上表现出较大的遗传多样性,但不同菌株存在着不同程度的亲缘关系。