2017—2019年江苏省兽医系统实验室检测能力比对

为了解兽医系统实验室检测能力和动物疫情监测预警水平,推进各级兽医实验室重视实验室检测工作,2017—2019年江苏省动物疫病预防控制中心组织全省兽医系统实验室开展检测能力比对工作。11个项目均采用国家标准或国家参考实验室推荐的方法进行比对。结果显示,各年度实验室正确率分别为38.64%、58.33%和93.51%,各年度样品正确率分别为89.08%、93.86%和99.32%;3个年度比对结果均正确的实验室22个,占比28.57%。结果提示各级畜牧兽医主管部门应重视兽医系统检测能力比对工作,加强实验室管理体系建设,提高检测人员业务能力,保证检测结果的正确率,提高动物疫病检测预警水平,从而为提升江苏省兽医系统实验室检测能力奠定基础。     

2019—2021年北京市兽医系统实验室检测能力比对

为了解兽医系统实验室检测能力，2019—2021年北京市动物疫病预防控制中心对12个区级动物疫病预防控制中心开展了检测能力比对。结果显示，各年度实验室符合率分别为75.00%、83.33%、100%，各年度样品符合率分别为98.02%、99.21%、100%。比对结果表明，北京市大部分兽医实验室的检测能力较强，个别实验室还需提高ELISA、F-PCR的试验能力。建议北京市各级畜牧兽医主管部门持续重视兽医系统检测能力比对工作，加强实验室管理体系建设，提高检测人员业务能力，保证检测结果的准确度，提高动物疫病监测预警水平。     

2019年辽宁省兽医系统实验室检测能力比对结果分析

为检验辽宁省兽医系统实验室整体检测能力,并促进其进一步提升,辽宁省农业发展服务中心组织实施了2019年度全省14个地市、24个生猪调出大县及33个其他县级兽医实验室检测能力比对试验,每个实验室参加3个比对项目,每个项目设置盲样6份。结果总体实验室正确率和样品正确率分别为94.37%（67/71）和99.61%（1 273/1 278）,14个地市级实验室正确率达100%,但有4个县级实验室的2个血清学检测项目上出现检测结果偏差较大。结果表明辽宁省兽医系统实验室基本具备完成相关检测项目的能力,但市、县级实验室之间存在一定差距,个别县级实验室检测水平还有待提高。这提示应重点加大对县级兽医系统实验室建设的投入,加强实验室管理体系建设,提升实验室管理运行规范化水平,进一步提升实验室检测人员业务能力,确保检测结果准确可靠。     

2019年山东省兽医系统实验室检测能力比对

2019年山东省动物疫病预防控制中心组织全省98个兽医系统实验室开展检测能力比对工作,比对项目包括非洲猪瘟病毒核酸检测、禽流感病毒核酸检测、H5亚型禽流感病毒抗体检测和口蹄疫非结构蛋白抗体检测。比对结果显示:总体实验室正确率为70.41%(69/98),其中市级兽医系统实验室正确率为93.75%(15/16),国家动物疫情测报站正确率为68.75%(11/16),县级兽医系统实验室正确率为65.15%(43/66);非洲猪瘟病毒核酸检测、禽流感病毒核酸检测、H5亚型禽流感病毒抗体检测以及口蹄疫非结构蛋白抗体检测结果正确率分别为87.76%、85.71%、93.88%和97.96%。比对结果提示基层兽医系统实验室应提升检验人员的素质,加大实验室设施设备的投入和管理,结合开展社会化服务来推进实验室建设,确保检测结果客观准确。     

青海省2017—2019年州县级兽医系统实验室检测能力比对

为全面了解和提升兽医系统实验室检测能力,2017—2019年青海省动物疫病预防控制中心连续3年组织州县级兽医实验室开展检测能力比对工作。根据每年疫病的流行态势,设置PCR、ELISA以及传统类检测等比对项目。结果显示:2017—2019年实验室总体准确率分别为50.00%、56.82%、74.47%,样品总正确率分别为90.75%、93.56%、93.48%,整体呈上升趋势;各实验室PCR、ELISA、传统类检测项目的正确率差异不显著(P>0.05);州级实验室的检测正确率明显好于县级(P<0.05)。结果表明,州县级实验室检测能力逐年提升,绝大多数实验室具备PCR、ELASA、传统类检测等检测能力,但部分县级实验室的个别项目检测能力有待提高。建议进一步加大经费投入,加强基层实验室管理、业务培训等,整体提升县级实验室的检测能力。     

2019年黑龙江省兽医实验室检测能力比对

2019年黑龙江省动物疫病预防与控制中心对全省13个市（地）、67个县（市、区）的80个兽医实验室开展了检测能力比对，比对项目包括禽流感抗体检测、猪瘟抗体检测、布鲁氏菌抗体检测、非洲猪瘟病毒核酸检测。结果显示，6个市级实验室和14个县级实验室比对结果正确率为100%，禽流感抗体、猪瘟抗体、布鲁氏菌抗体、非洲猪瘟病毒核酸检测项目结果正确率分别为71.2%、97.3%、73.3%、97.0%。比对结果客观反映了全省兽医系统实验室检测能力，提示应进一步加强实验室建设，加大资金投入，提升技术人员业务能力，规范实验室检测流程。     

2014—2021年云南省普洱市兽医系统实验室检测能力比对

为确保兽医系统实验室检测结果的科学性和准确性，普洱市动物疫病预防控制中心积极组织辖区内兽医系统实验室参与检测能力比对工作。2014—2021年，在全市共开展了44个比对项目检测。比对结果显示：参与检测能力比对的实验室数量由4个增至10个；市级动物疫病预防控制机构实验室、边境动物疫情监测站实验室和县（区）级动物疫病预防控制机构实验室的检测结果平均正确率分别为97.3%、95.7%、95.9%。以上结果表明，普洱市兽医系统实验室以考促建成效明显，整体检测水平较高，能够为动物疫病风险评估及防控体系提供有力支撑。针对现存的HA-HI检测能力薄弱、病原学检测能力不足等问题，建议相关部门培养专业素质强的实验工作人员，加大经费投入力度，加快管理体系建设，以进一步提升兽医系统实验室综合水平。     

国内要闻

2013年度兽医系统实验窒检潮能力比对工作全面完成 近日,由农业部组织开展的2013年度兽医系统实验室检测能力比对工作全面完成。今年的比对工作紧密结合国家和地方动物疫病监测计划要求,涵盖了禽流感、口蹄疫等重大动物疫病以及布鲁氏菌等人畜共患病的抗原、抗体检测项目,以全面考查实验室RT-PCR、ELISA、HA—HI等检测技术水平。全国31个省级动物疫病预防控制中心兽医实验室以及1200多个地（市）、县级动物疫病预防控制中心兽医实验室参加了比对,检测结果准确率、实验活动规范程度较前两年有了明显提高。     

2018—2022年云南省兽医系统实验室检测能力比对

为全面了解云南省兽医系统实验室检测能力和水平,提高各级兽医实验室监测预警和风险评估能力,2018—2022年云南省动物疫病预防控制中心组织全省16个州(市)级动物疫病预防控制中心实验室(简称州市级实验室)、30个国家动物疫情测报站/边境动物疫情监测站实验室(简称测报站/监测站)、79个已通过兽医系统实验室考核的县(区)级兽医实验室(简称县区级实验室)开展了检测能力比对工作。11个检测项目均采用国家标准进行比对。结果显示:各年份实验室正确率为52.27%～70.97%,样品正确率为94.04%～96.06%;连续5年全部正确的实验室有7个,此外还有10个实验室连续3年全部正确。结果表明,全省大部分兽医系统实验室具备了相应的检测能力和水平,且整体水平呈上升趋势,能够为云南省动物疫情监测预警和风险评估提供有力支撑,但部分县区级实验室及个别测报站/监测站仍有不足,尤其在HA-HI检测项目上还有待进一步提高。建议继续加强实验室管理,强化实验技能培训,提升检测人员的能力和水平,提高检测结果的准确性,积极支持有条件的测报站/监测站、县区级实验室开展分子生物学检测,以进一步提高全省兽医系统实验室检测水...     

2013—2015年贵州省市、县两级兽医实验室检测能力比对考核结果分析

对2013—2015年由贵州省动物疫病预防控制中心组织实施市、县两级兽医实验室检测能力比对考核工作结果进行分析,参加比对考核的市级兽医实验室9个,占全省市级实验室的100%(9/9),参加比对考核的县级兽医实验室60个,占全省县级兽医实验室的68.2%(60/88),全省比对考核的实验室占市县实验室总数的71.1%(69/97).比对考核实验涉及ELISA、布病虎红平板凝集试验、RT-PCR和H5亚型-HI 4个检测项目,均是当前动物疫病诊断与监测的重要检测方法,3年中ELISA、布病虎红平板凝集试验、RT-PCR、H5亚型-HI的正确率分别是92.8%、86.7%、84%、80.5%.表明本省市级实验室基本掌握了血清学和病原学检测,县级实验室也基本掌握了血清学检测,但与全国省级实验室检测平均水平比较还有一定差距,需加强专项技能培训与学习.     

Heartworm in dogs in Canada in 1984

In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.

Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.

     

Heartworm in dogs in Canada in 1988

In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%).     

Heartworm in dogs in Canada in 1985

In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).

Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1983

In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.

Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.

     

Heartworm in dogs in Canada in 1989

In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.

Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.

     

Heartworm in dogs in Canada in 1987

In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%).     

Heartworm in dogs in Canada in 1986

In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).

Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.