干扰素在犬病临床中的应用

一、干扰素的基本特点 （一）具有严格的种属特性：即某种动物细胞产生的干扰素,只能作用于同种动物的细胞,使其建立抗病毒状态。例如用犬的细胞生产的干扰素,只对犬有抗病毒作用。 （二）作用广谱性：干扰素对机体细胞的抗病毒作用一旦形成,就能获得抗多种病毒的能力。     

干扰素在犬类病毒性疾病治疗中的作用

<正>干扰素在治疗中具有良好的抗病毒和抑制病毒复制等作用,尤其是对犬瘟热、犬细小病毒病、犬传染性肝炎和犬冠状病毒病等疾病的治疗具有一定的疗效。一、干扰素作用机理干扰素(Interferon,简称IFN)是一组具有多种功能的活性蛋白质(主要是糖蛋白),是一种由单核细胞和淋巴细胞产生的细胞因子。它们在同种细胞上具有广谱的抗病毒、影响细胞生长以及分化、调节免疫功能等多种生物活性。干扰素是一种广谱     

禽干扰素基因工程表达技术研究进展

干扰素是一组具有多种功能的活性蛋白(主要是糖蛋白),是一种由单核细胞和淋巴细胞产生的细胞因子。根据干扰素产生的来源和结构不同可分为α干扰素、β干扰素和γ干扰素三类。研究发现干扰素通过细胞表面受体作用使细胞产生抗病毒蛋白,从而抑制病毒的复制,同时它还可增强自然杀伤细胞(NK细胞)、巨噬细胞和T淋巴细胞的活力,从而起到免疫调节作用,并增强抗病毒能力。由于干扰素几乎能抵抗所有病毒引起的感染,加     

干扰素对犬病的辅助治疗作用

干扰素（Ｉｎｔｅｒｆｅｒｏｎ，ＩＦＮ）是一种糖蛋白。由致敏猪淋巴细胞产生的商品干扰素制剂称为猪白细胞干扰素，是一种高活性、多功能的生物活性制品。干扰素是抗病毒感染的非特异性免疫的一个重要因素，能干扰几乎所有病毒的复制，保护未感染细胞抵抗病毒的攻击。干扰素对于免疫应答也有显著的调节作用，并可抗肿瘤。干扰素虽然是一种广谱的抗病毒活性物质，但却具有较严格的种属特异性。为阐明猪白细胞干扰素对其他种属动物的抗病毒作用，我们从１９９８－１０～１９９９－０１期间，选用四川世红生物技术有限公司生产的猪白细胞干扰…     

合理使用干扰素提高畜禽免疫力

<正>干扰素(IFN)是一种广谱抗病毒剂,并不直接杀伤或抑制病毒,而主要是通过细胞表面受体作用使细胞产生抗病毒蛋白,从而抑制乙肝病毒的复制;同时还可增强自然杀伤细胞(NK细胞)、巨噬细胞和T淋巴细胞的活力,从而起到免疫调节作用,并增强抗病毒能力。干扰素是一组具有多种功能的活性蛋白质(主要是糖蛋白),是一种由单核细胞和淋巴细胞产生的细胞因子。它们在同种细胞上具有广谱的抗病毒、影响细胞生长,以及分化、调节免疫功能等多种生物活性。     

鸭α-干扰素在昆虫细胞中的表达

干扰素（Interferon，IFN）是活细胞在病毒或其他干扰素诱生剂作用下产生的一种糖蛋白，当它进入未感染的细胞时，可诱导该细胞产生抗病毒蛋白质，从而抑制其他病毒在该细胞中的复制。干扰素具有抗病毒、抗肿瘤和调节免疫功能作用。无论哺乳类还是禽类，干扰素都有两大类型，即Ⅰ型（主要是α和β）和Ⅱ型（γ）干扰素。其中α干扰素（IFN-α）由白细胞产生。Ⅰ型于扰素的抗病毒作用最强，主要是通过旁分泌发挥作用。     

重组鸡α-干扰素的表达及其生物活性的初步研究

为获得重组鸡α-干扰素并对其进行生物活性研究,本试验对重组大肠杆菌进行诱导表达,收集重组鸡α-干扰素包涵体后进行复性纯化及免疫印迹试验,并通过测定病毒EID₅₀,在接种H9亚型禽流感病毒、新城疫病毒和传染性支气管炎病毒的鸡胚中进行干扰素抗病毒活性试验,运用微变量细胞病变抑制法进行重组鸡α-干扰素抗病毒效价的测定。结果显示复性后重组鸡α-干扰素的免疫印迹试验成功获得了预期条带,在鸡胚中重组鸡α-干扰素具有显著的抗H9亚型禽流感病毒、新城疫病毒和传染性支气管炎病毒等活性作用。在CEF/VSV细胞系上测定重组鸡α-干扰素的抗病毒效价为1.5×2¹⁰ U/mg左右,高浓度重组鸡α-干扰素具有一定的细胞毒性。结果表明重组鸡α-干扰素蛋白具有免疫原性和抗原性,在鸡胚内具有较好的抑制或杀灭H9亚型禽流感病毒、新城疫病毒和传染性支气管炎病毒的效果,呈广谱性,抗病毒效价较高,为下一步抗病毒制品的创制奠定基础。     

猪干扰素在临床生产中的应用

干扰素是由干扰素产生细胞（interferon—producing cells,IPCs）所分泌的一类具有高度生物学活性的糖蛋白,具有广谱的抗病毒、抗肿瘤、抗细胞增殖和免疫调节等功能。目前,猪病毒性传染病依然是制约养猪业发展的一类重要且难以控制的疾病,由于猪干扰素对猪病毒性传染病具有防御和抑制作用,有关猪干扰素的应用研...     

白羽鹌鹑IFN-α基因克隆、遗传衍化及原核表达研究

<正>干扰素(IFN)是一种具有广谱抗病毒、抗肿瘤和免疫调节等功能的细胞因子,可以通过调节机体的某些细胞来间接发挥抗病毒生物学作用,抑制病毒生长,促进免疫细胞活性。IFN-α属于Ⅰ型干扰素,是由白细胞分泌的一类具有抗病毒和免疫调节功能的细胞因子~([1])。IFN-α与靶细胞的表面受体结合后可以激活靶细胞内潜在抗病毒基因的表达,产生具有三磷酸鸟苷(GTP)酶活性的抗病毒蛋白质,使细胞具备     

鸭-干扰素在昆虫细胞中的表达

干扰素(Interferon,IFN)是活细胞在病毒或其他干扰素诱生剂作用下产生的一种糖蛋白,当它进入未感染的细胞时,可诱导该细胞产生抗病毒蛋白质,从而抑制其他病毒在该细胞中的复制[1]。干扰素具有抗病毒、抗肿瘤和调节免疫功能作用。无论哺乳类还是禽类,干扰素都有两大类型,即型(主要是α和β)和型(γ)干扰素。其中α干扰素(IFN-α)由白细胞产生。I型干扰素的抗病毒作用最强,主要是通过旁分泌发挥作用[2]。鸭I型干扰素基因是在1995年由Schults等成功克隆的[3,4]。他们使用已克隆的鸡型干扰素基因1(ChIFN1)作为探针,基因组DNA Southern杂…     

Prostatic squamous metaplasia in a cat with interstitial cell neoplasia in a retained testis

An 11-year-old cat with a retained testis was presented with a chronic history of dysuria and bladder atony. Medical therapy failed to alleviate the clinical signs. Contrast radiography demonstrated a diffusely narrowed urethra. During a celiotomy and prepubic urethrostomy, a retained testis, stenosed urethra, and irregularly enlarged prostate were observed. Histopathologic diagnosis was retained testis with a well-differentiated interstitial cell tumor, a poorly differentiated interstitial cell tumor, and marked squamous metaplasia of the prostatic epithelium with suppurative prostatitis. Neoplastic interstitial cells were immunoreactive for Melan A, consistent with reports of Melan A expression in steroid hormone-producing tissue. This is the first report of prostatic squamous metaplasia associated with testicular neoplasia in a felid.     

Results of excision of thymoma in cats and dogs: 20 cases (1984-2005)

OBJECTIVE: To provide long-term follow-up information for a series of dogs and cats with invasive and noninvasive thymomas treated by excision alone. DESIGN: Retrospective case series. ANIMALS: 9 cats and 11 dogs with thymoma. PROCEDURES: Medical records were reviewed. The following factors were analyzed for their effect on prognosis: age of dog or cat, invasiveness of the tumor, percentage of lymphocytes in the mass (percentage lymphocyte composition) on histologic evaluation, and mitotic index of the mass. RESULTS: All patients were treated with excision of the tumor alone. Median overall survival time for the cats was 1,825 days, with a 1-year survival rate of 89% and a 3-year survival rate of 74%. Median overall survival time for the dogs was 790 days, with a 1-year survival rate of 64% and a 3-year survival rate of 42%. Recurrence of thymoma was observed in 2 cats and 1 dog, and a second surgery was performed in each, with subsequent survival times of 5, 3, and 4 years following the first surgery. Percentage lymphocyte composition of the mass was the only factor that was significantly correlated with survival time; animals with a high percentage of lymphocytes lived longer. CONCLUSIONS AND CLINICAL RELEVANCE: Results of this study indicated that most cats and dogs with thymomas did well after excision. Even cats and dogs with invasive masses that survived the surgery and the few cats and dogs with recurrent thymomas or paraneoplastic syndromes had a good long-term outcome. Excision should be considered an effective treatment option for dogs and cats with thymomas.     

A Comparison of Three Techniques for Closure of Pelvic Flexure Enterotomies in Normal Equine Colon

Six enterotomies 3 cm long, spaced at 3 cm intervals, were made in the antimesenteric border of the pelvic flexure of the ascending colon in five adult horses. Ten incisions each were sutured with a Utrecht pattern (single layer), a full-thickness simple continuous oversewn with a Cushing pattern (two layer), and a mucosal simple continuous with a seromuscular simple continuous oversewn with a Cushing pattern (three layer). In all horses, chromic gut and polyglactin 910 were each used once for each pattern. On day 6, the pelvic flexure was excised and the colonic vessels were injected with a radio-opaque mass. Sections of each enterotomy were studied by light microscopy and high detail radiography. The single-layer closure led to serosa-to-mucosa healing and a thin bowel wall, and the three-layer closure resulted in distortion of the bowel wall by hematoma formation and submucosal contamination with ingesta. The two-layer closure resulted in good alignment and improved healing. There was less inflammatory reaction with polyglactin 910 than with chromic gut.     

不同前作对冬牧70黑麦产量和品质的影响

在南京地区研究了不同前作(水稻Oryza satica和杂交狼尾草Pennisetum americanum×P. purpureum)对冬牧70黑麦Secale cereale var. Dongmu 70产量、再生情况和营养品质的影响.结果表明:水稻茬种植的冬牧70黑麦较杂交狼尾草茬种植的再生茎蘖数多,株高高,干物质积累多;水稻茬冬牧70黑麦,2次刈割鲜干比也明显比杂交狼尾草茬的高,第2次刈割的茎叶比较杂交狼尾草茬的低.因此,在水稻茬种植冬牧70黑麦产量较高,品质也较好.     

A rapid method for preparing avian tracheal rings for organ culture

A simple, rapid method for preparing rings from chicken tracheas is described. Using a polyacrylamide disc gel slicer, up to 100 uniform tracheal rings were obtained from two tracheas with a single cut and without injury to the ciliated tracheal epithelium. The responses of rings cut with a disc gel slicer and those cut conventionally with a scalpel blade were compared by virus titration, virus neutralization, and viability of uninfected 18-day-old-chick embryo rings over a nine-day incubation period. Rings cut with a disc gel slicer did not differ significantly from those cut conventionally with respect to virus titer, neutralization indices, or viability. A disc gel slicer expedites the preparation of functional rings and offers an alternative to the present method of cutting tracheal rings with a scalpel blade.     

Taenia coenurus in the orbit of a chinchilla

A 4-year old, male intact, captive-bred chinchilla (Chinchilla lanigera) was presented due to progressive exophthalmos of the right eye over a 5-month period. Ophthalmic examination revealed exophthalmos with dorsal displacement of the right globe. Retropulsion was decreased and a fluctuant, subcutaneous mass could be palpated posterior and dorsal to the central aspect of the zygomatic bone. Transdermal ultrasonography revealed a fluid-filled mass consistent with a cyst located within the ventral right orbit. Computed tomography demonstrated dorsal displacement of the globe, lateral displacement of the zygomatic arch, and numerous mineral-dense foci within the lumen of the cyst. The cyst was removed en bloc by ventral transpalpebral orbitotomy. Histopathology revealed a single capsulated cyst with multiple invaginated protoscolices, characterized by a prominent scolex with refractile hooklets, suckers, and abundant calcareous corpuscles consistent with a Taenia coenurus. Exophthalmos resolved with surgical therapy and there was no evidence of recurrence or postoperative complications over a period of 2 years. To the authors' knowledge, this is the first reported case of an orbital cyst of parasitic origin in a chinchilla.     

Hydrometra in a ferret-case report.

A desexed female ferret was presented with bilateral alopecic changes. Clinical examination revealed generalized alopecia and abdominal distension. A polycystic mass was found behind the right kidney, and the whole abdomen was filled with a large turgid mass. Radiography and ultrasonography confirmed the presumptive diagnosis of a hydrometra. Hematology and serum biochemistry showed regenerative anemia with light azotemia. Laparotomy showed the presence of a neoplastic mass at the location of the right ovary, a massive enlargement of the uterus filled with a clear fluid, and a subcapsular cyst on the left kidney. After surgery, histopathologic examination of the tissues diagnosed a leiomyoma of the right ovary with hyperplasia of the uterine wall.     

Cattle with thymic lymphoma and haematoma of the ventral neck: a comparison of findings

The goal of the present paper was to establish criteria for the differentiation of thymic lymphoma and haematoma of the ventral neck in cattle. Four cattle with thymic lymphoma and seven with haematoma of the ventral neck underwent physical, haematological, biochemical and ultrasonographic examinations as well as histological evaluation of a biopsy specimen. All of the animals had firm non-painful swellings, which were no warmer than normal and extended from the pharyngeal region to the thoracic inlet. A diagnosis could not be established based on the results of physical examination and palpation of the swellings. Animals with a haematoma had a shorter history of being ill compared to those with thymic lymphoma. In patients with a haematoma, anaemia was present, the heart rate was elevated and the haematocrit was decreased, but not in cattle with thymic lymphoma. A tentative diagnosis was made on the basis of a macroscopic evaluation of the biopsy samples, which were dark red and firm in cattle with a haematoma. All cases of malignant thymic lymphoma were confirmed by histological examination of a biopsy sample. Ultrasonography did not help to differentiate between haematoma and thymic lymphoma. All animals with thymic lymphoma and three of those with a haematoma were euthanased. The four remaining animals with a haematoma were treated successfully.     

Efficacy of a continuous, multiagent chemotherapeutic protocol versus a short-term single-agent protocol in dogs with lymphoma

OBJECTIVE: To compare response rates and remission and survival times in dogs with lymphoma treated with a continuous, multiagent, doxorubicin-based chemotherapeutic protocol or with a short-term single-agent protocol incorporating doxorubicin. DESIGN: Nonrandomized controlled clinical trial. ANIMALS: 114 dogs with lymphoma. PROCEDURES: Dogs were treated with a chemotherapeutic protocol consisting of L-asparaginase, vincristine, cyclophosphamide, doxorubicin, methotrexate, and prednisolone (n=87) or doxorubicin alone (27). RESULTS: 63 of 86 (73%) dogs treated with the multiagent protocol (data on response was unavailable for 1 dog) and 14 of 27 (52%) dogs treated with the single-agent protocol had a complete remission. Dogs with lymphoma classified as substage相似文献   

Detection of serum alpha-fetoprotein in dogs with naturally occurring malignant neoplasia

Serum alpha-fetoprotein (AFP) concentrations were determined, by use of an automated microparticle enzyme-linked immunoassay (MEIA), in 16 control dogs and 48 dogs with previously untreated, histologically confirmed, naturally occurring neoplasia (17 dogs with lymphoma and 31 dogs with nonhematopoietic malignancies, 13 dogs with carcinomas, 18 dogs with sarcomas). Mean serum AFP concentrations for untreated dogs with lymphoma, for dogs with sarcomas, and for dogs with carcinomas were not significantly different from the mean serum AFP concentration for the 16 untreated control dogs. Mean serum AFP concentration for dogs with transitional cell carcinoma (n=7) was not significantly different from the mean serum AFP concentration for the control dogs. It has been shown previously by others that a mean serum AFP concentration > 225 ng/mL is suggestive of a hepatic malignancy (e.g., hepatocellular carcinoma, lymphoma). In our study, all 48 dogs had a normal complete blood count, serum biochemical analysis, and urinalysis. Only one of the 48 dogs was found to have a serum AFP concentration > 225 ng/mL. Later evaluation of this dog confirmed hepatic involvement with lymphoma. AFP can be detected in the serum of dogs with naturally occurring tumors using the MEIA technique. A serum AFP concentration above that observed in normal dogs is not a common finding in dogs with naturally occurring neoplasia; however, we confirmed that a serum AFP concentration > 225 ng/mL, with or without evidence of a serum biochemical abnormality, may suggest primary and/or secondary hepatic involvement with a neoplastic disease and may warrant an adjustment in clinical stage and prognosis. A prospective diagnostic and therapeutic evaluation of dogs, with naturally occurring tumors having an elevated serum AFP concentration would determine the validity of this conclusion.