密花柽柳(Tamarix arceuthoides)春夏两季种子的萌发行为

密花柽柳(Tamarix arceuthoides)具有两季开花结实特性,通过对春花期与夏花期种子的形态和萌发特性的比较,结果表明:密花柽柳春花期种子与夏花期种子在形状、大小、颜色上无显著差异。新采收的春花期种子与夏花期种子在5～15℃,5～25℃,15～25℃和25～35℃4个温变周期下都能够快速萌发,且春花期种子比夏花期种子的萌发速率快,春花期种子萌发率达到50%仅需要2～36h。浓度低于0.2mol/L的NaCl溶液对密花柽柳春花期种子和夏花期种子萌发的影响不大;但从0.4mol/L起萌发率随着浓度增高而降低,直至为0;将在盐溶液中处理8d的种子转移至蒸馏水后,萌发恢复率均为0,表明NaCl处理后的春花期种子和夏花期种子永久地失去萌发力。室温储存2个月密花柽柳春花期种子和夏花期种子的活力保持在80%以上,之后随着贮藏时间的延长种子活力逐渐下降,贮藏6个月时春花期和夏花期的种子活力均下降40%～50%。     

温度、盐分和储藏时间对多花柽柳种子萌发的影响

通过研究储藏时间、温度以及不同浓度NaCl溶液对多花柽柳种子萌发的影响,结果表明:多花柽柳种子褐色,棒状,长(0.368±0.005)mm,宽(0.172±0.004)mm,千粒重(20.518±2.500)mg;种子寿命约240 d;在5/15 ℃,5/25 ℃,15/25 ℃和25/35 ℃(光/暗=12/12 h)4个温变周期下,多花柽柳新成熟种子的萌发率均在90%以上,且彼此间差异不显著;盐分浓度小于0.2 mol/L时不抑制种子萌发,大于0.6 mol/L时萌发率随盐浓度的增高而降低,直至为零,且复水后恢复萌发率低,表明NaC1处理后的部分种子永久地失去萌发力.最后探讨了多花柽柳种子的萌发行为对荒漠环境的适应意义.     

温度、盐分对木地肤Kochia prostrata种子萌发的影响

以分布于准噶尔荒漠的木地肤为材料,研究了木地肤种子在4个温变周期(5/2℃、15/2℃、25/15℃、30/15℃,光12 h/暗12 h)下以及0.0~4.0mol/L NaCl溶液中的萌发。结果表明:木地肤种子萌发的适宜温周期为30/15℃和25/15℃,萌发率均大于85%;低温周期(5/2℃、15/2℃)明显抑制种子的萌发。低浓度的NaCl盐溶液(≤0.1mol/L)促进木地肤种子萌发,有一定的增效效应;小于0.6mol/LNaCl盐溶液对种子累积萌发率(≥80%)无显著影响;NaCl浓度大于1mol/L后,随着盐浓度的增加,盐度与萌发率呈显著负相关关系;盐胁迫解除后,种子具有一定的恢复萌发能力,随着盐浓度的不断增加,种子的恢复萌发率逐渐下降;在NaCl盐溶液高达2.0、4.0mol/L时,恢复萌发率为36%和39.6%。     

不同类型盐分对盐爪爪种子萌发和幼苗生长的影响

采用培养皿法研究不同类型钠盐胁迫对荒漠植物盐爪爪种子萌发率、萌发指数、幼苗芽长和根长,分析盐爪爪种子萌发与幼苗生长适应不同类型盐碱地的机制,为种植盐爪爪生态修复不同类型盐碱地提供了科学依据。结果表明:(1)NaCl、Na2SO4、NaHCO3、Na2CO3均显著抑制盐爪爪种子的萌发率和萌发指数。(2)回归方程分析盐爪爪萌发期的耐NaCl、Na_2SO_4、NaHCO_3、Na_2CO_3临界值分别为325 mmol·L-1、267 mmol·L-1、245 mmol·L~(-1)和166 mmol·L~(-1),其极限值分别是671 mmol·L-1、580 mmol·L~(-1)、569 mmol·L~(-1)和389 mmol·L-1,相对于中性盐,盐爪爪种子萌发时对碱性盐更敏感。(3)NaCl、Na_2SO_4、NaHCO_3、Na_2CO_3均显著抑制盐爪爪幼苗芽和根的生长,盐爪爪种子萌发和幼苗生长对这4种钠盐的响应差异显著;相对于幼苗芽,幼苗根的响应更为敏感,可将根长作为评价盐爪爪耐盐性的参数,碱性盐毒害强于中性盐;根据盐分对盐爪爪种子萌发和幼苗生长的毒害性顺序依次为:NaClNa_2SO_4NaHCO_3Na_2CO_3。     

褪黑素浸种对大豆种子萌发过程中干旱胁迫的缓解效应

以大豆干旱敏感型品种绥农26为材料,采用室内培养法,研究不同浓度褪黑素(0、100、300、500、800μmol·L~(-1))浸种对6%PEG-6000模拟干旱胁迫条件下大豆种子萌发的影响。结果表明:干旱胁迫处理抑制了大豆种子萌发,与对照(蒸馏水处理种子)相比,处理D(6%PEG处理种子)的发芽势、发芽率、根体积和鲜重显著降低,降幅分别为38.14%、23.72%、21.4%、25.6%;根系电导率、MDA和H_2O_2含量大幅上升,增幅分别为51.18%、83.33%和60.60%。而褪黑素浸种处理(100、300、500、800μmol·L~(-1))显著缓解了干旱胁迫对种子萌发的抑制,促进了根系生长,其中以根直径和干重提升最为显著,分别增加了16.38%、29.73%、34.97%、31.72%和10.70%、14.28%、10.70%、25.00%;抗氧化酶(SOD、POD、CAT、APX)活性提高,其中处理M500+D(500μmol·L~(-1)褪黑素+6%PEG)效果最为显著,增幅分别为37.61%、40.10%、12.96%和28.31%。在6%PEG-6000模拟的干旱胁迫下,500μmol·L~(-1)褪黑素浸种处理对大豆种子萌发过程中干旱胁迫的缓解效果最佳。     

不同外源调节物质对低温胁迫下玉米的缓解效应分析

脯氨酸(Pro)和甜菜碱(GB)是一类重要的渗透调节物质,其与低温胁迫下植物的抗逆性密切相关。为了探讨不同浓度的外源Pro或GB对低温胁迫下玉米种子萌发、幼苗性状的影响,综合评价Pro或GB的低温缓解效果。本试验以3份寒敏感自交系为试材,分别在萌发期和苗期10℃低温胁迫下,用5种不同浓度的Pro或GB进行浸种、培养作为试验处理;25℃和10℃下以双蒸水浸种、培养作为正向、负向对照处理CK(+)、CK(-)。结果表明:(1)低温胁迫下,玉米种子的发芽势、发芽率、胚芽长、胚根长、胚芽鲜重和胚根鲜重显著降低;幼苗的相对电导率、丙二醛、脯氨酸及可溶性糖含量显著升高,SOD、POD及CAT活性显著增强。(2)添加外源Pro或GB后,除幼苗相对电导率和丙二醛含量明显降低外,其余5个幼苗性状和6个萌发性状都明显升高。(3)低温胁迫下,6个萌发性状和7个幼苗生理生化性状在自交系间差异显著,在Pro或GB浓度间差异显著,在自交系与浓度互作间差异显著。(4)利用低温缓解指数EI作为综合评价指标,并采用隶属函数法对外源Pro或GB的低温缓解效果进行综合评价表明,萌发期600、800μmol·L~(-1)Pro;20μmol·L~(-1)GB的综合低温缓解效果最佳,为0.882或0.647,1000μmol·L~(-1)Pro或50μmol·L~(-1)GB的综合缓解效果最小为0.513或0.022;苗期400μmol·L~(-1)Pro或10μmol·L~(-1)GB的综合低温缓解效果最佳为0.577或0.500,1000μmol·L~(-1)Pro或40μmol·L~(-1)GB的综合低温缓解效果最小仅为0.246或0.289;萌发期和苗期,外源Pro的综合低温缓解效果分别是GB的1.858倍和1.064倍。     

NaCl和Na2SO4胁迫对白菜型冬油菜种子萌发的影响及其耐盐性分析

以6个耐盐性不同的白菜型冬油菜种子为材料,将NaCl和Na_2SO_4按摩尔比1:1混合,设浓度为0(CK)、45、90、135、180、240 mmol·L~(-1)6个处理,测定其对种子萌发和生长参数的影响,研究不同冬油菜种子萌发时的耐盐能力,以期为耐盐冬油菜的筛选提供依据。结果表明:(1)45 mmol·L~(-1)和90 mmol·L~(-1)盐浓度对种子萌发的影响不明显;135 mmol·L~(-1)和180 mmol·L~(-1)盐浓度明显抑制了种子萌发,表现为种子发芽势、发芽率、发芽指数、活力指数、胚根长、胚芽长、鲜重和干重均随盐浓度的增大而明显降低。因此,135~180 mmol·L~(-1)盐浓度可认为是耐盐性鉴定的适宜范围。(2)不同指标对盐胁迫的敏感程度差异较大,发芽指数、活力指数、胚芽长和鲜重与盐浓度均为极显著负相关,对盐胁迫最为敏感。(3)3个耐盐冬油菜ky-1、ky-10-191和ky-NDJ的种子发芽指数、活力指数、胚芽长和鲜重的耐盐临界值显著高于原有品系平油1号、10-191和ky-NDJ。ky-1、ky-10-191、ky-NDJ的耐盐临界值分别为133.895、123.264、107.399 mmol·L~(-1),而平油1号、10-191、NDJ的耐盐临界值分别为117.394、103.947、93.834 mmol·L~(-1)。用隶属函数法综合评价,耐盐性强弱顺序依次为ky-1ky-10-191平油1号kyNDJ10-191NDJ。可见,ky-1、ky-10-191和ky-NDJ的耐盐性较平油1号、10-191和NDJ均有所提高。     

温周期及果翅对梭梭种子萌发行为的调控

种子萌发行为的受控机制是生殖生态学的重要研究内容.通过对适宜的温周期下,不同贮藏时期果翅对梭梭种子萌发影响的研究.结果表明:梭梭种子在温周期为5℃/25℃,5℃/15℃和15℃/25℃(暗/光=12 h/12h)中均能快速萌发.果翅对秋天新成熟种子的萌发有显著的抑制作用,使种子处于强迫休眠状态;果翅对种子萌发的抑制作用随着贮藏时间的推移逐渐降低,到翌年春天(4月)解除这种抑制作用.果翅对梭梭种子萌发行为的调控作用确保了种子在合适的时间萌发与种群的成功定居,这也是梭梭在荒漠地区广泛分布的主要原因.     

外源硫化氢对加工番茄种子耐盐性及抗氧化酶的影响

为明确硫化氢对盐胁迫下加工番茄种子萌发的缓解作用,用Na Cl半致死浓度为85 mmol·L~(-1)的KT-63(耐盐性弱)和半致死浓度为120 mmol·L~(-1)的KT-18(耐盐性强)为材料,用各自的半致死浓度与不同浓度的NaHS(0.2,0.4,0.6,1.0 mmol·L~(-1))分别配成混合液处理种子。结果表明,随着NaHS浓度的增加,种子的发芽率、发芽势、发芽指数、活力指数、SOD、POD、CAT活性呈先上升后下降的趋势,其中与盐胁迫单独处理相比,用0.4mmol·L~(-1)NaHS处理,种子的发芽率、发芽势、发芽指数、活力指数、SOD、POD、CAT活性在KT-63中分别提升70%、250%、120%、130%、6.7%、150%和180%,在KT-18中分别提升50%、250%、60%、80%、4.3%、160%和200%,而MDA的含量显著降低,并且对KT-63的缓解效应强于KT-18。综上所述,H2S(0.4 mmol·L~(-1))有效缓解了加工番茄萌发过程中盐胁迫的抑制作用,促进了种子的萌发。     

外来有害植物土荆芥种子贮藏与萌发特性的研究

研究了不同光照和温度对外来有害植物土荆芥（Chenopodium ambrosioides L．）种子萌发率的影响及种子在不同贮藏时间和不同贮藏温度下萌发率的变化。结果表明，光照是土荆芥种子萌发的必要条件，全黑暗条件下萌发率仅为9．7％。种子在15～20℃恒温条件下萌发率均达到80％以上，25℃恒温和10℃恒温能显著抑制其萌发。零下低温（-20℃）和零上低温（4℃）贮藏3个月的土荆芥种子萌发率分别为86．3％和84．7％，与贮藏前萌发率（89．3％）相比无显著差异，而在室温和25℃条件下贮藏的种子，萌发率显著下降。     

Germination strategies of annual and short-lived perennial species in the Arabian Desert

Germination timing is highly regulated in short-lived plant species since it strongly influences recruitment success of vegetation. In deserts, the spatiotemporal distribution of plant-available water is highly episodic and unpredictable, making winter months more favorable for seed germination when other abiotic conditions co-occur. We hypothesized that changes in photoperiod and thermoperiod would impact germination more in seeds that had undergone in situ storage. We assessed 21 annual and short-lived perennial species in the Arabian Desert to find (1) if seeds were dormant at maturity, (2) if in situ seed storage increased germination percentage compared with no storage, (3) if photoperiod and thermoperiod germination requirements were influenced by in situ storage, and (4) if a phylogenetic association in seed germination could be observed. Seeds of each species collected in early 2017 were divided into two batches. One was tested for germination within one week (fresh seeds). The other was stored in situ at the maternal location (stored seeds) until October 2017 and tested for seed germination in the first week of November. Seed germination was conducted in incubators at two thermoperiods (15°C/20°C and 20°C/30°C; 12 h/12 h), and two photoperiods (12 and 0 h light per day). Results indicated that seed germination percentages of 13 species were significantly enhanced by in situ storage. A thermoperiod response was exhibited by stored, but not fresh seeds. Light exposure increased germination of fresh seeds but had only a minimal effect on stored seeds. Germination traits exhibited no phylogenetic correlation. This result indicated that selection pressure for germination strategy was stronger than that for taxonomic traits of these desert species.     

Eco-physiological studies on desert plants: germination of Halothamnus iraqensis Botsch. seeds under different conditions

With the aim to investigate if the halophyte Halothamnus iraqensis Botsch. can be suitable for re-vegetation and remediation of salt-affected lands, this study evaluated(1) the effects of photoperiod, thermoperiod, storage period and wings' presence on its seed germination, and(2) the ability of its seeds to have successful germination recovery after salt stress. Germination tests in different photoperiods(12 h light/12 h darkness and total darkness) and thermoperiods(15℃/20℃ and 20℃/25℃) were conducted for seeds collected in 2012, 2013, 2014, 2015 and 2016. The seeds collected in 2016 were sown under different salinity levels(0, 100, 200, 400 and 600 m M Na Cl) to assess the salinity tolerance during the germination. Wings' presence highly inhibited seed germination of this species in both photoperiods and thermoperiods under all salinity level treatments. In addition, the germination recovery occurred well when seeds were deprived of their wings. The photoperiod of 12 h light/12 h darkness and the thermoperiod of 15℃/20℃ were the best conditions for seed germination. Germination percentages of H. iraqensis seeds decreased with the increasing storage duration, especially after three years of the collection. In addition, H. iraqensis seeds were able to germinate under different salinity levels, and their germination percentages decreased with increasing salinity levels. H. iraqensis seeds have the ability to recover their germination after alleviating the salt stress, irrespective of photoperiod, highlighting the halophilous character of this species.     

Assessment of daily heat pulse regimes on the germination of six Amaranthus species

In Mediterranean environments, thermal requirements for seed germination of Amaranthus species are met in spring. Nonetheless, seedlings of these species start emerging in the field from late winter, although the theoretical heat sum thresholds required for germination are not likely to be reached under field conditions. We hypothesised that soil thermal fluctuations can reduce the heat requirements of these species. Cardinal temperatures and thermal times of six Amaranthus species (A. albus, A. cruentus, A. deflexus, A. graecizans, A. retroflexus and A. viridis) were determined on non‐dormant seeds at constant temperatures ranging from 10 to 40°C. Subsequently, germination response to heat pulses was studied by imposing a thermal fluctuation regime of 32/8°C with two different thermoperiods of 3/21 and 6/18 h. In the two thermoperiods, exposure to 32°C was imposed for a different number of days: from 1 to 12 and from 1 to 6 heat pulses cycles in the 3/21 and 6/18 h thermoperiods respectively. Cumulative germination, germination rate and mean germination time were evaluated. Heat sum requirements and final germination percentage were affected by thermoperiod and number of thermal cycles. Amaranthus spp. germination was higher and faster when seeds were submitted to 6 h compared with 3 + 3 h of heat pulses. Our data showed that heat sum requirements for germination may change depending on the way in which varying temperatures are imposed on germinating seeds.     

Maternal salinity improves yield,size and stress tolerance of Suaeda fruticosa seeds

Shrubby seablite or lani(Suaeda fruticosa Forssk)is a perennial euhalophyte with succulent leaves,which could be planted on arid-saline lands for restoration and cultivated as a non-conventional edible or cash crop.Knowledge about the impacts of maternal saline environment on seed attributes of this important euhalophyte is lacking.This study investigated the effects of maternal salinity on yield,size and stress tolerance of S.fruticosa seeds.Seedlings of S.fruticosa were grown in a green net house under increasing maternal salinity levels(0,300,600 and 900 mM NaCl)until seed production.Total yield,size,stress tolerance and germination of the descended seeds under different maternal saline conditions were examined.Plants grown under saline conditions(300,600 and 900 mM NaCl)produce a substantially higher quantity of seeds than plants grown under non-saline condition(0 mM NaCl).Low maternal salinity(300 mM NaCl)improves seed size.Seeds produced under all maternal salinity levels display a higher tolerance to low temperature(night/day thermoperiod of 10℃/20℃),whereas seeds produced under 300 mM NaCl maternal saline condition show a better tolerance to high temperature(night/day thermoperiod of 25℃/35℃)during germination.Seeds from all maternal saline conditions germinate better in the 12 h photoperiod(12 h light/12 h dark)than in the dark(24 h dark);however,seeds produced from low and moderate maternal saline conditions(300 and 600 mM NaCl)show a higher germination in the dark than those from control and high maternal saline conditions(0 and 900 mM NaCl).In general,maternal salinity is found to improve yield,size and stress tolerance of S.fruticosa seeds.     

Seasonal changes in germination responses of buried seeds of the weedy summer annual grass Setaria glauca

Seeds of Setaria glauca (L.) Beauv. buried in soil and exposed to natural temperature cycles exhibited seasonal changes in temperature, but generally not light; dark requirements for germination. Seeds were dormant at maturity in late September and October (autumn), and during burial from October to January they entered conditional dormancy, germinating up to ≥60% in light and darkness at daily thermoperiods of 25/15,30/15 and 35/20^C by January. During burial from February to May or June, seeds became non-dormant and germinated up to 68–100% in light and darkness at 15/6,20/10,25/15,30/15 and 35/20^C in May or June. At maximum yearly temperatures in June or July–August, 65–89% of the seeds entered conditional dormancy (germinating at 30/15 and 35/20, but not at 15/6,20/10 and 25/15^C), and the others entered dormancy (not germinating at any thermoperiod). Thus, most buried seeds had an annual conditional dormancy/non-dormancy cycle, but some had an annual dormancy/non-dormancy cycle. Except for seeds buried in 1990 that lost the ability to germinate in darkness at all thermoperiods the first summer of burial, seeds incubated in light and in darkness exhibited the same patterns of seasonal changes in germination responses. Although conditionally dormant and non-dormant seeds germinated to high percentages in darkness in Petri dishes, seedlings were found only in bags of seeds exhumed in April and May 1983, indicating that some factor(s) associated with the burial environment other than darkness prevented germination of buried seeds.     

外源调节剂包衣对低温胁迫下棉花种子萌发及幼苗耐寒性的影响

为探讨外源调节剂包衣对低温胁迫棉花幼苗耐寒性的调控效应,通过室内砂培试验,研究了外源调节剂包衣处理对低温下棉花种子萌发的影响,分析了5℃低温胁迫下以及恢复常温后外源调节剂包衣对棉花幼苗耐寒性的生理响应。结果表明:低温胁迫下棉花种子的萌发和幼苗生长受到抑制,水杨酸以及外源调节剂复配包衣处理均能显著提高低温胁迫下棉花种子的发芽势、发芽率、发芽指数和活力指数,其中发芽指数和活力指数达到极显著水平,增幅分别为41.2%~44.4%和51.2%~63.9%;外源调节剂包衣处理叶片的相对电导率(REC)和MDA含量显著降低,可溶性蛋白和可溶性糖等渗透调节物质的含量显著提高,SOD、POD和CAT等抗氧化酶的活性也显著增强;5 mmol·L-1水杨酸+45 mmol·L-1氯化钙+60 mg·L-1亚硒酸钠复配包衣处理的叶片REC、MDA含量显著低于其它包衣处理,可溶性蛋白含量及其增幅显著高于其它包衣处理。说明水杨酸、氯化钙和亚硒酸钠三元复配包衣在缓解棉花幼苗低温逆境中具有协同效应,可以缓解低温胁迫对棉花种子萌发及幼苗生长的抑制作用,提高植物的耐寒性。     

青海云杉种子萌发特性

以天祝县青海云杉天然种群种子为研究对象,通过对种子生活力测定,分析种子萌发对温度、光照、水分和盐分胁迫的响应,为引种提供参考数据。结果表明：① 种子的生活力为72%。② 在黑暗条件下,温度小于10 ℃种子不萌发,最适萌发温度25/15 ℃(72%);种子在24 h黑暗和24 h光照条件下的萌发率显著高于14 h光照/10 h黑暗。③ 当NaCl胁迫浓度为50 mmol·L^-1时,可促进种子萌发,当胁迫浓度大于50 mmol·L^-1时,抑制种子萌发;胁迫解除后,随着胁迫浓度的增加,恢复萌发率(RGR)先增加后减小,胁迫浓度为400 mmol·L^-1时,RGR达到最大值13%。④ 随着PEG-6000试剂胁迫渗透势的增加,种子萌发显著减少,在-0.9 MPa时,种子不能萌发;胁迫解除后,RGR先增加后减少,在-0.9 MPa时,达到最大值35%。     

Effects of water stress and NaCl stress on different life cycle stages of the cold desert annual Lachnoloma lehmannii in China

For a plant species to complete its life cycle in arid and saline environments, each stage of the life cycle must be tolerant to the harsh environmental conditions. The aim of the study was to determine the effects of water stress (water potentials of -0.05, -0.16, -0.33, -0.56, -0.85 and -1.21 MPa) and NaCl stress (50, 100, 200, 300, 400, 500 and 600 mmol/L NaCl) on seed germination percentage, seedling survival and growth, juvenile growth and plant reproduction of Lachnoloma lehmannii Bunge (Brassicaceae), an cold desert annual that grows in the Junggar Basin of Xinjiang, China in 2010. Results indicated that low water stress (-0.05 and -0.16 MPa) had no significant effect on seed germination percentage. With a decrease in water potential, germination percentage decreased, and no seeds germinated at -0.85 and -1.21 MPa water stresses. Germination percentage of seeds was significantly affected by NaCl stress, and higher germination percentages were observed under non-saline than saline conditions. An increase in NaCl concentrations progressively inhibited seed germination percentage, and no seeds germinated at ≥400 mmol/L NaCl concentration. Non-germinated seeds were transferred from both PEG (polyethylene glycol-6000) and NaCl solutions to distilled water for seed germination recovery. The number of surviving seedlings and their heights and root lengths significantly decreased as NaCl stress increased. About 30% of the plants survived and produced fruits/seeds at 200 mmol/L NaCl concentration. Thus, seed germination, seedling establishment and reproductive stage in the life cycle of L. lehmannii are water- and salt-tolerant, with seedlings being the least tolerant. These tolerances help explain why this species can survive and produce seeds in arid and saline habitats.     

恶性杂草马齿苋（Portulaca oleracea）种子萌发特性的研究

为了明确恶性杂草马齿苋种子的萌发特性,本文研究了温度、光照、pH、水势、盐分及土层深度对其种子萌发的影响。结果表明：马齿苋种子在光照L∥D=14 h∥10 h、温度白天32 ℃,晚上28 ℃的变温条件下萌发率最高,为92%。马齿苋种子萌发适宜的酸碱度范围较宽,在5相似文献   

影响丰花草种子萌发和幼苗生长的主要环境因子

运用培养皿滤纸法和盆栽法,分别研究温度、光照和水分条件对热带常见杂草丰花草种子萌发和幼苗生长的影响。结果表明:丰花草种子萌发温度范围广泛(5~40℃),随温度升高,丰花草幼苗的鲜质量和生长长度均呈现先升高后降低的趋势;恒温下30℃为其种子萌发的最适温度,其发芽率、发芽指数、活力指数均相对较高,而平均发芽时间最短;25℃时生长的幼苗最健壮,其芽长、根长和芽鲜质量、根鲜质量均显著高于其他温度时的对应值。而变温条件更有利于丰花草种子萌发,12 h D/12 h L、5℃/15℃时发芽率达55.63%,15℃/25℃时发芽率90%,25℃/35℃时的种子萌发率、幼苗长度、幼苗鲜质量均最大。光照不是影响丰花草种子萌发的关键因子,但光照可能有利于促进丰花草幼苗生物量向根部分配,光暗交替(12 h D/12 h L)条件下丰花草幼苗生长情况相对最好(长度根冠比为1.05∶1;鲜质量根冠比为0.51∶1)。水分是决定丰花草种子能否萌发的关键因素,25℃、12 h D/12 h L条件下,在土壤最大持水量60%时丰花草种子萌发和幼苗生长状况均最佳。