新疆野苹果(Malus sieversii)种群年龄结构及其动态特征

新疆野苹果（Malus sieversii）是我国珍贵的濒危野生植物。通过在伊犁河谷的那孜、大莫合沟、那拉提和交托海4个新疆野苹果分布区建立样地,绘制年龄结构图、编制静态生命表,采用生存分析探明种群的存在状况,应用时间序列分析预测种群发展趋势。结果表明：新疆野苹果年龄结构呈中部膨大,幼龄个体少,中老龄个体多,那孜种群年龄结构偏大,交托海种群偏小;与其他种群相比,大莫合沟V＇pi最小,随机干扰风险极大值（Pmax）接近0,对外界干扰较敏感;那拉提种群的区间寿命、总寿命和期望寿命低,消失率高,种群衰退早;不同种群的存活曲线趋于Deevey-Ⅱ型,表明各龄级的死亡率相近;随着龄级增加,种群死亡密度曲线先下降后上升,危险率函数在Ⅳ龄级后增加明显,说明环境压力对野苹果的影响在Ⅳ龄级后表现强烈;受虫害、旅游、放牧干扰影响,时间序列分析预测野苹果种群老龄个体数量增加,中、幼龄个体缺乏补充,呈现不同程度衰退。应采取人工促萌或补植幼苗,调控林地密度,控制虫害蔓延,加强野苹果种质资源保护。     

氮添加对新疆野苹果幼苗枝叶大小的影响

新疆野苹果(Malus sieversii)是全世界栽培苹果的祖先之一,也是我国重要的战略性植物种质资源,但目前其种群退化严重,对新疆野苹果进行保育需要了解其对氮添加的响应.对不同水平氮添加处理的新疆野苹果幼苗小枝的枝叶大小性状进行单因素方差分析以及异速生长分析,以探明新疆野苹果对氮添加处理的响应,为种群恢复提供理论基...     

孑遗植物新疆野苹果种群生命表与生存分析

应用”空间代替时间”及匀滑技术,编制新疆野苹果种群特定时间生命表,绘制其死亡曲线和存活曲线,分析其种群数量动态变化.结果表明:新疆野苹果种群处于生命力相对旺盛的中龄期(Ⅴ、Ⅵ龄级比例最高),但因幼龄个体数目稀少而存在衰退趋势;新疆野苹果的死亡率和消失率随着龄级的增加呈现先减小后增加的趋势,种群死亡率在Ⅰ龄级最高(83.2％),Ⅶ龄级最小(28.6％),种群动态表现为前期衰退、中期相对稳定、后期不稳定的特点.     

利用高通量测序检测新疆野苹果上的病毒

新疆野苹果Malus sieversii (Ledeb.) Roem.是苹果的祖先, 也是重要的育种遗传资源, 常用作砧木。近年来, 新疆野苹果的分布面积骤减, 对其的保护越来越重要。本研究采用高通量测序技术, 对采自新疆伊犁天山野果林的26份样本进行病毒检测, 检测到苹果褪绿叶斑病毒(apple chlorotic leaf spot virus, ACLSV)和苹果茎痘病毒(apple stem pitting virus, ASPV)。通过RT-PCR检测所有样品(127份), 检测到ACLSV、ASPV和苹果茎沟病毒(apple stem grooving virus, ASGV), 检出率分别为22%、19.7%和11%。结合RACE PCR技术扩增得到ASGV新疆野苹果分离物基因组全长序列, 暂时命名为ASGV-XJ。去除3′末端poly(A)后的长度为6 506 bp, 有两个彼此重叠的开放阅读框(open reading frame, ORF), ORF1(47-6 364 nt)编码一个241 kD的多聚蛋白, 包含甲基转移酶(methyltransferase)、木瓜蛋白酶(papain-like-protease)、解旋酶(nucleotide triphosphate-binding helicase)、RNA 依赖的RNA聚合酶(RNA-dependent RNA polymerase)和C端的外壳蛋白(coat protein, CP)等; ORF2(4 798-5 760 nt)编码一个36 kD的运动蛋白(movement protein, MP)。系统发育分析表明, 分离物间没有表现出寄主专一性和地理分布规律。上述结果对新疆野苹果的保护工作有重要的参考意义。     

死亡植株对新疆野苹果种群遗传多样性的影响

新疆野苹果（Malus sieversii）为国家二级保护植物和重要的野生果树种质资源。选取遭受小吉丁虫等病虫害破坏最为严重的新源县种群为对象,利用微卫星（SSR）标记死亡植株和存活植株的基因型,对比两者间的遗传差异,探究树木死亡对新疆野苹果种群遗传多样性的影响。结果表明：（1）死亡植株与存活植株间没有显著的遗传分化,这说明新疆野苹果植株死亡不存在遗传相关性,为随机死亡过程;（2）树木死亡对新源县种群的遗传结构未产生重大影响,遗传多样性水平仅轻微减小,但丢失了许多低频度等位基因;（3）未来的监测和评价中,等位基因数（Na）比遗传多样性指数能更有效地反映新疆野苹果遗传资源变化。     

天然林中不同径级新疆野苹果的光合能力

研究新疆天山野果林建群种新疆野苹果（Malus sieversii）光合生理特性,对于该种群保育及其生态功能评价具有重要意义。通过野外测定新疆新源天然野果林中不同径级64株新疆野苹果南向枝条上部叶和下部叶饱和光强下的净光合速率（A）、气孔导度（g_s）和比叶面积（SLA）。结果表明：随着径级增加,上部叶和下部叶的A_area、A_mass显著增加。g_s随径级的增加先增加再降低,在径级Ⅲ（树干0.5 m高处直径25~35 cm）时达最大。上部叶比下部叶有着高的A_area、A_mass和g_s。回归分析表明：随着树干直径增加,A_area和g_s呈二次多项式增加,A_mass呈线性增加。SLA随径级增加变化不显著,上部叶SLA显著低于下部叶。上部叶的A_area高于下部叶,但由于SLA显著减小,在A_mass上两者差异减小。随g_s增加,上部叶Aarea呈幂函数增加,下部叶A_area呈二次多项式增加。      

新疆野苹果资源保护对策探讨

新疆野苹果是现代栽培苹果的原始祖先,也是苹果育种中的重要材料,具有极为重要的保护价值.但近几年由于对新疆野苹果林的不合理利用和管理不善等原因,导致了新疆野苹果资源的持续减少.根据实地调查和文献资料,对新疆野苹果林的主要威胁因素及其保护和研究状况进行了评估,认为超载放牧、不合理的嫁接改造、病虫危害及过度的果实采收是造成新疆野苹果林持续衰退的主要因素.现有保护区数量少、保护范围小、保护手段单一,难以满足对野苹果资源的保护需要.据此,提出了加强保护区和保护小区建设、健全野苹果林保护体系、加强新疆野苹果种质资源的收集和异地保护、促进野苹果林群落的更新恢复和保护、加强对野苹果林演替动态及更新机制等方面的建议.     

生境片段化对新疆野苹果种群遗传结构的影响

生境片段化对植物种群具有复杂的遗传效应。在地理种群内部,新疆野苹果(Malus sieversii)呈现出多个片段化小群体。本研究选取遗传多样性较高的巩留种群作为研究对象,利用微卫星(SSR)标记采自5个小群体样点的101个植株样品基因型。通过分析不同小群体间的遗传多样性差异与遗传谱系关系,来探究生境片段化对新疆野苹果种群遗传结构的影响。结果表明:5个小群体间存在着遗传多样性差异,其中处于边缘位置的萨哈和小莫合尔2小群体保存了高水平的遗传多样性;由于地理隔离效应作用,这5个小群体可以划分为东西两个不同的遗传谱系。建议在将来开展的新疆野苹果种质资源保育和核心种质库构建过程中,应当注重这些边缘小群体和不同的遗传谱系。     

世界栽培落叶果树起源中心--新疆天山伊犁谷地野果林

据实地调查,天山伊犁河谷地(含外伊犁山)野苹果林为17741 hm2;野杏林为1355 hm2,较位于哈萨克斯坦境内阿拉木图以西的天山塔拉斯阿拉套野苹果林1419 hm2;野杏林190 hm2,和卡拉套野杏林790 hm2的合计还多。据此可见,天山伊犁河谷地的孑遗植物(野果林)的面积远远大于天山阿拉木图以西。不仅如此,天山伊犁河谷地野果林还有相当数量的樱桃李、欧洲李(1980年林培钧教授发现于     

新疆野苹果枝条化学计量海拔变异特征研究

为揭示不同海拔新疆野苹果(Malus sieversii)养分化学计量特征的变异性,选择一条山谷作为固定研究区,在1300 m、1400 m、1500 m和1600 m等4个海拔梯度上连续3 a(2016—2018年)开展调查取样,对比分析野苹果一年生枝条N、P、K化学计量时空变异特征及其与环境的关系。结果表明:不同海拔和年份新疆野苹果枝条化学计量指标多为中、弱变异,其中P的变异性最弱,N:K变异性最强;年份对除P:K外的5个化学计量指标有显著影响,而海拔仅对N:P有显著影响;枝条N、N:P及N:K也受年份与海拔交互作用的影响。枝条P(2017年除外)、K及P:K在不同海拔间无显著差异,但均具有明显的年际变化;枝条N、N:P及N:K同时具有显著的年际和海拔变异性,其变化趋势各异。相关性分析和NMDS分析表明,野苹果自身生长状况(枯枝率、投影盖度、病虫害程度及结果量)、年降水量及土壤因子对枝条化学计量特征均有一定影响,但不同化学计量指标的响应特征不同;总体上,植株长势越好,其枝条养分含量越低。可见,野苹果枝条化学计量特征在不同海拔梯度和年际间具有明显的变异性,其影响因素较为复杂。     

新疆伊犁地区野果林的群落特征及保护

新疆伊犁地区是我国野果林分布的主要地段.通过野外调查和资料收集,共获得67个野果林群落样方,分析了新疆伊犁地区野果林的群落结构及其分布特征.结果表明:野果林林下物种丰富,共记录维管束植物46科141属207种.群落分类将野果林划分为野核桃林、野苹果林、野杏林和野生樱桃李林4种类型.野杏林和野生樱桃李林的物种丰富度显著高...     

Spatiotemporal variations in the growth status of declining wild apple trees in a narrow valley in the western Tianshan Mountains,China

Malus sieversii (wild apple tree), only distributed in the Tianshan Mountains in Central Asia, is a tertiary relic species and an ancestral species of cultivated apples. However, existing natural populations of wild apple trees have been declining. To date, spatiotemporal variations in the growth status of declining wild apple trees and influencing factors in the narrow valley areas in the Tianshan Mountains remain unclear. In this study, field investigation and sampling were carried out in three years (2016-2018) at four elevations (1300, 1400, 1500, and 1600 m) in the Qiaolakesai Valley (a typical longitudinal narrow valley in the Yili River Valley) of the western Tianshan Mountains in Xinyuan County, Xinjiang Uygur Autonomous Region, China. Projective coverage, dead branch percentage, and 18 twig traits (these 20 parameters were collectively referred to as plant traits) were determined to comprehensively reflect the growth status of declining wild apple trees. The values of dead branch percentage ranged from 36% to 59%, with a mean of 40%. Year generally showed higher impact on plant traits than elevation. In 2017 and 2018, projective coverage, leaf size, leaf nitrogen concentration, and nitrogen to phosphorous ratio were markedly higher than those in 2016. However, dead branch percentage and leaf and stem phosphorous concentrations showed the opposite trend. Most of the topological parameters of plant trait networks differed in the three years, but the strength of trait-trait association increased year by year. The mean difference between day and night temperatures (MDT), annual accumulative precipitation, soil electrical conductivity, and soil pH had the greatest impact on the plant trait matrix. The growth status of declining wild apple trees was directly and positively affected by MDT and leaf size. In conclusion, the growth of declining wild apple trees distributed in the narrow valley areas was more sensitive to interannual environmental changes than elevation changes. The results are of great significance for further revealing the decline mechanism of wild apple trees in the Tianshan Mountains.     

天山野果林生态系统受损现状及其保护

天山自然资源丰富,是新疆人民世世代代赖以生存和发展的天然宝库,野果林生态系统是其重要的组成部分, 国干旱     

新疆部分地区苹果、核桃和杨树腐烂病的病原鉴定

 树木腐烂病在新疆主林果种植区均有不同程度的发生,近年呈加重趋势。本研究在调查了新疆阿克苏、和田、伊犁、喀什和库尔勒等地区苹果树、核桃树和杨树3种林果腐烂病田间发病情况的基础上,重点对14个样本点的样品进行病原物的分离与鉴定。结果表明,各样本点3种林果均有不同程度的腐烂病发生,分离鉴定结果为引起苹果树腐烂病病原菌主要为Valsa mali var. mali(分离频率为64.5%),其次是V. mali var. pyri(25.8%)、V. malicola(3.2%)和V. nivea(6.5%);引起核桃树和杨树腐烂病病原菌为V. sordida(分离频率为83.3%和96%)和V. nivea(分离频率为16.7%和4%)。离体枝条及苹果果实上的致病性测定结果表明,3种林果上分离得到的病原菌均能感染原寄主。引起新疆部分地区苹果树腐烂病的主要病原菌是Valsa mali var. mali,核桃树和杨树腐烂病的主要病原菌是V. sordida。同时,基于实际生产中存在感病枯死杨树枝干作为苹果园、核桃园树体支撑木的现象,推测3种林果腐烂病菌间可能存在交叉感染的潜在风险。     

Genetic structure and historical demography of Malus sieversii in the Yili Valley and the western mountains of the Junggar Basin,Xinjiang, China

 Malus sieversii, a wild progenitor of domesticated apple, is distributed in western Xinjiang of China, eastern part of Kazakhstan and Kyrgyzstan in Central Asia. To well understand the genetic structure and the historical demography of this important germplasm resource, we sampled 15 populations with 110 individuals of Malus sieversii from the Yili Valley and the western mountains of the Junggar Basin, Xinjiang, and sequenced two nrDNA fragments for these 110 individuals. Meanwhile, we modeled and compared species distributions under the current and the Last Glacial Maximum climatic conditions. The results showed that populations of M. sieversii from Xinjiang had low levels of genetic diversity and genetic differentiation. During the LGM period, populations of M. sieversii had lost their northern distributions in the western mountains of the Junggar Basin. M. sieversii has experienced a demographic expansion from the south of the Yili Valley to the north of the western mountains of the Junggar Basin during the warm interglacial epochs. Due to the high sensibility of M. sieversii to disturbance, we proposed more attention should be paid to the M. sieversii populations in the western mountains of the Junggar Basin.     

Estimation of net primary productivity and its driving factors in the Ili River Valley,China

Net primary productivity(NPP), as an important variable and ecological indicator in grassland ecosystems, can reflect environmental change and the carbon budget level. The Ili River Valley is a wetland nestled in the hinterland of the Eurasian continent, which responds sensitively to the global climate change. Understanding carbon budget and their responses to climate change in the ecosystem of Ili River Valley has a significant effect on the adaptability of future climate change and sustainable development. In this study, we calculated the NPP and analyzed its spatio-temporal pattern of the Ili River Valley during the period 2000–2014 using the normalized difference vegetation index(NDVI) and an improved Carnegie-Ames-Stanford(CASA) model. Results indicate that validation showed a good performance of CASA over the study region, with an overall coefficient of determination(R2) of 0.65 and root mean square error(RMSE) of 20.86 g C/(m~2·a). Temporally, annual NPP of the Ili River Valley was 599.19 g C/(m~2·a) and showed a decreasing trend from 2000 to 2014, with an annual decrease rate of –3.51 g C/(m~2·a). However, the spatial variation was not consistent, in which 55.69% of the areas showed a decreasing tendency, 12.60% of the areas remained relatively stable and 31.71% appeared an increasing tendency. In addition, the decreasing trends in NPP were not continuous throughout the 15-year period, which was likely being caused by a shift in climate conditions. Precipitation was found to be the dominant climatic factor that controlled the inter-annual variability in NPP. Furthermore, the correlations between NPP and climate factors differed along the vertical zonal. In the medium-high altitudes of the Ili River Valley, the NPP was positively correlated to precipitation and negatively correlated to temperature and net radiation. In the low-altitude valley and high-altitude mountain areas, the NPP showed a negative correlation with precipitation and a weakly positive correlation with temperature and net radiation. The results suggested that the vegetation of the Ili River Valley degraded in recent years, and there was a more complex mechanism of local hydrothermal redistribution that controlled the growth of vegetation in this valley ecosystem.     

伊犁野果林植物多样性及其保护

在中国仅分布于新疆伊犁，塔城等地的天山野果林是苹果，核桃，杏，李等多种果树的起源地之一，新疆野苹果，野核桃，野巴旦均已被列为中国濒危二级保护植物。根据多年的调查和采集，初步统计分布在伊犁野果林中仅种子植物就有４３０余种，其中野生果树植物有７科１７属４８种，此外还分布有许多苔藓类，地衣类以及大型真菌等丰富的微生物资源，野果林又是多种鸟类和动物活动，繁衍的优良栖息地。     

Association of phytoplasmas with the decline of European hazel in southern Italy

In the Campania region of southern ltaly. commercial orchards of European hazel ( Corylus avellana ) are severely affected by yellowing and decline. To determine whether phytoplasmas are associated with the disorder, stem samples from diseased trees were examined using polymerase chain reaction assays. No visible products were obtained by amplification of sample DNA with universal and group-specific phytoplasma primers. However, when the products obtained with universal primers were re-amplified with nested primers that were specific for the fruit tree phytoplasmas of the apple proliferation group, most samples tested positively. Restriction site analysis revealed that the trees were infected with the apple proliferation, pear decline, and European stone fruit yellows phytoplasmas in about the same proportion. Some of the trees were doubly infected with one of the fruit tree phytoplasmas and the aster yellows agent. Most of the infected trees were also identified by hybridization of the products obtained in the initial amplification with suitable oligonucleotide probes.     

Seed and pollen transmission of <Emphasis Type="Italic">Apple latent spherical virus</Emphasis> in apple

To examine whether Apple latent spherical virus (ALSV) has spread among apple trees in an orchard, we surveyed 21 apple trees surrounding two ALSV-infected trees for virus infection using a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). None of the 21 trees were infected, indicating that ALSV has not spread from the infected trees to the neighboring apple trees since it was first detected in 1984. We analyzed seed embryos and seedlings derived from infected trees and detected ALSV in 10 of 223 seed embryos (4.5%) and 10 of 227 seedlings (4.4%). From these results, we conclude that ALSV is seed-transmitted at a rate of ca. 4.5% in apple. We also analyzed seed embryos and seedlings from uninfected apple trees that were hand-pollinated with pollen from infected trees. We detected ALSV in only 1 of 260 seed embryos and in none of the 227 apple seedlings. This result indicated that the seed transmission rate via infected pollen is only 0–0.38%. In situ hybridization analysis of ALSV-infected apple flower buds showed that ALSV was present inside almost all pollen grains and in all ovary and ovule tissues, including the embryo sac and inner integument.     

新疆野苹果和秦冠的抗黑星病特性

 透射电镜观察表明, 苹果叶片上表皮角质层厚度在品种间存在显著差异, 新疆野苹果和秦冠叶片的角质层厚度显著高于富士和嘎啦的;同一品种不同龄期叶片的角质层厚度随着叶龄增长而增厚, 且黑星病严重度与叶片上表皮角质层厚度间存在显著的负相关关系。苹果品种抗病性组分分析结果表明, 新疆野苹果的病害严重度最低, 约为嘎啦的1/22, 潜育期最长, 为嘎啦的2.0倍, 无(或少有)病斑出现, 不产孢。秦冠的严重度约为嘎啦的1/14, 潜育期约为嘎啦的1.5倍, 产孢量约为嘎啦的1/26。黑星病菌在新疆野苹果和秦冠叶片上的侵染概率及病斑扩展速率均显著低于富士和嘎啦。因此, 新疆野苹果和秦冠对黑星病的抗病性表现在抗侵入和抗扩展两个方面。