海南岛上岸渔获调查与分析

上岸渔获调查是调查渔业资源的主要方法之一。通过2010年9月至2011年8月监测海南岛主要渔港渔情,统计分析渔获上岸量、渔获组成和作业类型,评估南海渔业资源开发现状并提出建议。结果表明,1）低值幼鱼渔获比例超过40％,经济渔获蓝圆鲹（Decapterusmaruadsi）、带鱼（Trichiurushaumela）、马鲛（Scomber-morus）、眼镜鱼（Menemaculata）、金线鱼（Nemipterus）和头足类（Cephalopoda）的总和不足25％;2）蓝圆骖和带鱼是海南岛上岸渔获的优势种;3）拖网是海南岛近海渔获量最高的作业类型;4）海南岛近海底层渔业资源已严重衰退,远海中上层渔业资源具有巨大开发潜力。建议禁止海南岛近海拖网生产,允许南海休渔期间灯光围网和灯光罩网生产,通过增加远海作业船只的柴油补贴等途径扶持灯光围网和灯光罩网生产,构建“养护近海渔业、开发远海渔业”的新捕捞格局。     

闽南-台湾浅滩渔场单船拖网作业调查与分析

根据2009—2010年闽南-台湾浅滩渔场单船拖网作业监测调查资料和生产统计数据,阐明单船拖网作业的渔业地位、渔场分布、网具类型,着重分析该作业渔获种类组成及主要捕捞对象的资源动态。结果表明:单船拖网作业产量位居闽南-台湾浅滩渔场各种海洋捕捞作业的首位,大吨位、大功率渔船数量连续多年不断增长;渔具型式以有翼单囊网具为主;部分渔船使用的拖网网囊尺寸远远低于国家标准;带鱼、二长棘鲷幼鱼损害现象较为严重;秋季8—10月蓝圆鲹和鲐鱼群体绝大多数个体可达到最小可捕规格。调查研究结果还表明,2002年以来单船拖网渔业资源结构已发生较大变化,生命周期短、营养级较低的蟹类、枪乌贼类、乌贼类和虾类资源渔获比重显著增长;原优势种类绿布氏筋鱼资源已出现严重衰竭;二长棘鲷资源量逐年减少,渔获群体明显趋向小型化、低龄化。     

福建底拖网捕捞能力的分析

根据2002年福建闽南地区双船底拖网和单船底拖网的生产调查及全省渔业统计，结合相关的渔捞记录，应用DEA法，分析了福建各地，市底拖网的捕捞能力和闽南地区不同作业方式捕捞能力及其影响因素，并与单位捕捞努力量渔获量计算进行比较。结果表明：福建各地、市实际拥有底拖网的渔船数量、功率和吨位可能达到的最大产出量的“能力利用度”差异悬殊，1999年和2001年最低的仅为19．4％、19．9％；全省底拖网平均“能力利用度”偏低，1999年和2001年分别为64．3％、67．5％，全省底拖网作业仍然存在渔船的投人数量过多、功率和吨位偏大的问题；因捕捞方式不同，影响捕捞能力的因子有所不同，但投入功率和作业天数都是影响双船底拖网和单船底拖网捕捞能力的主要因素；在考虑将单位捕捞努力量渔获量数据转换为“能力利用度”时，其前提条件是计算采用的因子必须一致。但单位捕捞努力量渔获量在考虑计算投入因子的数量上有很大的局限性，采用DEA法可获得各种投入要素下捕捞能力变化的信息，结果比较符合客观实际。     

南海北部马面鲀渔获率初步研究

根据2009-2014年在南海北部生产的183艘渔船的渔捞日志,分析了南海北部海域马面鲀渔获率随作业方式、年际分布与季节分布间的变化特征。分析结果表明,渔获马面鲀主要作业类型为双拖、单拖、桁杆拖虾、围网、定置张网和刺网6种,其中单拖、双拖网作业渔获率较高;2009-2014年,南海北部马面鲀渔获率低于南海南部,整体渔获率呈逐年递减趋势,仅2011年渔获率突增,2014年马面鲀渔获率降为最低。马面鲀常年主要分布于珠江口、粤西、海南岛沿岸及北部湾海域,粤东海域偶有分布。海南岛沿岸、粤西及珠江口海域渔获率密度较大,北部湾和粤东海域渔获率密度相对较小。各区域渔获率季节变化明显,渔获率最大值常在夏季出现,春、秋季次之,冬季最小。引起渔获率差异的主要原因为自然条件与捕捞压力。本文通过对马面鲀渔获率变化及原因分析,为南海北部马面鲀合理捕捞提供依据。     

珠江口虾拖网的副渔获组成

拖虾是南海捕捞渔业的一种主要作业方式,南海北部的珠江口是广东省虾拖网的优良渔场之一。文章以珠江口铜鼓水道虾拖网渔业生物调查资料为基础,对该渔业的副渔获组成进行了分析研究。结果表明,珠江口虾拖网渔获种类多,以底栖鱼类和甲壳类为优势类群;副渔获的组成在不同月份有所变化;副渔获比例较高,虾与副渔获之比为1:10-1:19;副渔获中大部分鱼种未达到可捕规格,对幼鱼资源造成损害。建议渔业管理部门重视对副渔获问题的调查研究。     

四片式拖网技术推广研究报告

八十年代以来,南海拖网渔业发展迅速,渔船数量猛增,渔获量逐年增大。但渔船单位功率产量连年下降,说明底鱼资源正在衰退。相反,中下层鱼类资源捕捞潜力依然巨大。于是,1988～1989年我们进行了南海机轮单船四片式拖网的研究,并获得成功,且正式通过了专家技术鉴定。 材料和方法 推广应用的网具是在1988～1989年研制的单船四片式拖网的基础上经过进一步优化设计制作成具有南海各地特     

我国当前南海南部拖网渔业的困局和对策

南海南部渔场是广西拖网渔船在南海外海的传统作业区域之一。然而,近年来该海域渔业涉外事故频发,广西渔船损失惨重。为了规避涉外事故,广西拖网渔船不得不频频中断正常生产,转移到南海外海其它涉外风险较低的渔场作业。2013年5月以后,在南海外海生产的广西拖网渔船陆续撤出南海南部,至今未恢复生产。本文采用南海捕捞信息动态采集网络和信息渔船2013年的生产记录,通过统计及对比,分析拖网渔船从南海南部转移后面临的困境和成因。结果表明,渔船转移到6°N以北的南海外海渔场生产,单位捕捞努力量渔获量（CPUE）减少31.25%、优质鱼类所占的比例明显下降、全部航次亏损,生产难以为继。目前南海南部的生产态势明显不利于南海外海渔业的发展。本文提出恢复和坚持南海南部生产是南海外海拖网渔业发展的基础,有关部门应加强渔场巡航护渔、组织综合编队生产及加大对渔船开发南海南部渔业资源的支持力度。为了进一步发展南海外海拖网生产,建议在南海南部探索试验共同渔区模式。     

渤海口虾蛄三重刺网渔获组成及其捕捞性能分析

为研究渤海口虾蛄(Oratosquilla oratoria)三重刺网的捕捞性能,以河北省昌黎县的口虾蛄三重刺网为基础,于2013年4、5、7月在河北省秦皇岛近海进行50 mm、55 mm、60 mm和65 mm 4种网目尺寸的三重刺网作业性能比较实验,并与60 mm网目单片刺网和底拖网进行渔获对比分析。结果表明:三重刺网具有捕获渔场中绝大多数渔业种类的能力,选择能力较差。春季的渔获种类19种,为渤海近岸底层种类;夏季的渔获种类19种,除了小黄鱼(Pseudosciaena polyactis)和带鱼(Trichiurus lepturus)等底层鱼类外,还有日本鲭(Scomber japonicus)和蓝点马鲛(Scomberomorus niphonius)等中上层鱼类。春季的渔获结构单一,口虾蛄占渔获量的90%以上,主要兼捕种类鲜明鼓虾(Alpheus distinguendus)占4.1%,渔获选择性较好;夏季的渔获中,口虾蛄比例在75%左右,兼捕对象主要为小黄鱼、蓝点马鲛等经济鱼类的幼鱼,对渔业资源造成一定程度的损害,但渔获选择性比底拖网好。单片刺网的选择性最好,但渔获量低,只有三重刺网渔获量的30%左右,无法满足生产要求。建议开展渤海口虾蛄的可捕产量和分区域捕捞管理研究,开发作业性能更优越的口虾蛄专业捕捞渔具。     

南海大目拖网及其初步试验

80年代以来，南海底层鱼类资源正在衰退，渔船单位功率产量逐年下降，而且大部分为低值下杂鱼，优质鱼只占10％，渔船生产效益低，以致一些底拖网渔般被迫停产或转产，拖网渔业潜在崩溃危机。另一方面，南海渔场尤其海南岛周围水域中下层鱼类资源(如带鱼、马鲛鱼等)有待开发利用的潜力很大，     

科技与学术动态

南海新型大目拖网研制成功 改革开放以来,南海区底拖网渔业迅猛发展,大功率拖网渔船数量不断增加,拖网渔业总产量连年增长。然而底鱼资源却遭受严重破坏,许多经济鱼类资源随之日趋衰退,导致渔船单位功率产量趋于下降,渔船经济效益不佳。但是,据调查发现南海中层水域,尤其海南岛周围水域,中层鱼类的捕捞潜力依然巨大,可是现用的底拖网由于网目尺寸和网口周长太小,网口垂直张开受到限制,     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Protein and amino acid composition from the mantle of juvenile O ctopus vulgaris exposed to prolonged starvation

Protein and amino acid composition of the mantle of juvenile O ctopus vulgaris (Cuvier, 1797) during fasting for 27 days were determined. Average protein content of octopus mantle was of 711.19 ± 46.80 g kg^?1 DW, and it decreased with increasing fasting days. The non‐essential amino acids content was higher (486.18 ± 11.08 g kg^?1 protein) than essential amino acids (425.82 ± 9.15 g kg^?1 protein) at the start of the experiment (unstarved animals). The results suggest that the amino acid profile of the mantle where the most abundant amino acids are Arg, His, Lys, Gly, Leu and Pro could indicate a prolonged fasting condition (>20 days) or poor nutrition of O . vulgaris. This study supports the idea of using mantle for metabolic needs of starved O . vulgaris suggesting that the degradation pathway of amino acids to pyruvate and tricarboxylic acid cycle intermediates was favoured contrary to the degradation pathway of ketogenic amino acids. Special considerations should be taken concerning Thr, Ile, Ser, Ala, Asx (Asp, Asn), Glx (Glu, Gln) (because of their fast intake) and Lys and His (due to their stable contents) during a prolonged period of fasting.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

美人鱼发光杆菌对凡纳滨对虾非特异性 免疫功能及抗病力的影响

在基础饲料中分别添加0.1%和1%美人鱼发光杆菌灭活菌、0.1%美人鱼发光杆菌活菌配制成3种免疫实验饲料,以基础饲料为空白对照组饲料,每组设3个平行样。对个体质量为(4.83±0.36)g的凡纳滨对虾进行为期20 d的饲养实验,分别在0、5、10、15和20d进行取样,以血清中的酚氧化酶(PO)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)和溶菌酶(UL)活性为免疫指标,探讨了美人鱼发光杆菌作为免疫制剂对凡纳滨对虾非特异性免疫效应的影响;在投喂免疫饲料后的第22天,按0.004 2 kg/kg体重的剂量,直接投喂对虾白斑综合征病毒(WSSV)病料,并记录累积死亡率。结果表明,美人鱼发光杆菌免疫实验组对凡纳滨对虾血清中PO、ACP、AKP、UL和SOD活性影响明显高于对照组,并且在饲料中添加美人鱼发光杆菌后,明显提高了对虾抵御WSSV感染的能力。其中0.1%美人鱼发光杆菌活菌实验组的抗病毒感染能力最强,WSSV感染14d内累计死亡率为63.3%±5.8%;而对照组为96.7%±3.3%。研究表明,美人鱼发光杆菌添加在对虾饲料中能提高凡纳滨对虾非特异性免疫水平,增强抵抗疾病的能力,将其作为对虾免疫增强剂具有良好的应用前景。     

Effects of Spirulina platensis as a feed additive on growth and coloration of blue dolphin cichlids (Cyrtocara moorii Boulunger, 1902)

This study was carried out to investigate the effects of replacing fish meal with dietary Spirulina as a feed supplement on the growth performance and coloration of blue dolphin cichlids (Cyrtocara moorii). Five isonitrogenous (47% crude protein) and isocaloric (17.36 kJ/g digestible energy) diets were for formulated to replace FM with 0, 5, 10, 15 and 20% Spirulina (designated as Control, SP5, SP10, SP15 and SP20 respectively) and fed to the fish (initial body weight, 3.15 ± 0.01 g). Fish were randomly distributed into fifteen 120 L aquariums (26.5 ± 1.00°C), 15 fish per aquarium. The diets were tested in triplicate for 12 weeks. Experimental groups were fed twice daily (09:00 and 17:00) by hand to satiation. At the end of the feeding trial, significantly (p < 0.05) higher weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER) and lower feed conversion ratio (FCR) were observed in fish fed the SP10 diet when compared to the SP20 diet. There was no significant difference in these parameters between the other groups. The skin coloration of blue dolphin cichlid fed a diet containing Spirulina meal was enhanced. The best coloration was observed in the SP15 group. These impressions were objectively validated by chemical determinations of carotenoids extracted from fish skins and passed statistical tests of significance. The study findings show that Spirulina meal does not diminish growth rates except at very high levels.     

Dietary thiamin and pyridoxine requirements of fingerling Indian major carp,Cirrhinus mrigala (Hamilton)

Two experiments were conducted to quantify the dietary thiamin (experiment I) and pyridoxine (experiment II) requirements of fingerling Cirrhinus mrigala for 16 weeks. In experiment I, dietary thiamin requirement was determined by feeding seven casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) with graded levels of thiamin (0, 0.5, 1, 2, 4, 8 and 16 mg kg^?1 diet) to triplicate groups of fish (6.15 ± 0.37 cm; 1.89 ± 0.12 g). Fish fed diet with 2 mg kg^?1 thiamin had highest specific growth rate (SGR), protein retention (PR), RNA/DNA ratio, haemoglobin (Hb), haematocrit (Hct), RBCs and best feed conversion ratio (FCR). However, highest liver thiamin concentration was recorded in fish fed 4 mg thiamin kg^?1 diet. Broken‐line analysis of SGR, PR and liver thiamin concentrations exhibited the thiamin requirement in the range of 1.79–3.34 mg kg^?1 diet (0.096–0.179 μg thiamin kJ^?1 gross energy). In experiment II, six casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) containing graded levels of pyridoxine (0, 2, 4, 6, 8 and 10 mg kg^?1 diet) were fed to triplicate groups of fish (6.35 ± 0.37 cm; 1.97 ± 0.12 g). Fish fed diet containing 6 mg kg^?1 pyridoxine showed best SGR, FCR, PR, RNA/DNA ratio, Hb, Hct and RBCs, whereas maximum liver pyridoxine concentration was recorded in fish fed 8 mg kg^?1 dietary pyridoxine. Broken‐line analysis of SGR, PR and liver pyridoxine concentrations reflected the pyridoxine requirement from 5.63 to 8.61 mg kg^?1 diet. Data generated during this study would be useful in formulating thiamin‐ and pyridoxine‐balanced feeds for the intensive culture of this fish.     

Sea bream culture in Egypt; status, constraints and potential

Marine fish farming in Egypt began in 1976 with the culture of gilthead sea bream, (Sparus aurata) as this fish was notably adaptable to brackish and marine pond conditions. Today, marine fish and shrimp farms amount to about 19,000 ha, out of which 42% is already in production while the rest, i.e., 58% is still under construction. In 1997, cultured gilthead sea bream production of 2,250 tons made up 3% of the 75,000 tons total aquaculture catch. In polyculture, usually with the grey mullet and sea bass, gilthead sea bream contributed 440 kg ha^–1 to the total yield of 1,700 kg ha^–1 (26%) over a period of 16 months. For the same period, the yield of monoculture ponds averaged 100 kg ha^–1, while in marine cages, yields ranged from 4–10 kg m³. In 1996–1997, fry of 0.25–1 g and fingerlings 1–10 g with a total of 3 million, were collected from the wild and 1 million fry were produced in the three marine hatcheries out of the four existing ones. The development of sea bream culture in Egypt is now severely inhibited by the shortage of seeds and adequate feeds. Exports of both sea bream and sea bass, during 1994–1996 averaged 1,300 tons per year.     

A Minchinia mercenariae‐like parasite infects cockles Cerastoderma edule in Galicia (NW Spain)

The cockle Cerastoderma edule fishery has traditionally been the most important shellfish species in terms of biomass in Galicia (NW Spain). In the course of a survey of the histopathological conditions affecting this species in the Ria of Arousa, a haplosporidan parasite that had not been observed in Galicia was detected in one of the most productive cockle beds of Galicia. Uni‐ and binucleate cells and multinucleate plasmodia were observed in the connective tissue mainly in the digestive area, gills and gonad. The parasite showed low prevalence, and it was not associated with abnormal cockle mortality. Molecular identification showed that this parasite was closely related to the haplosporidan Minchinia mercenariae that had been reported infecting hard clams Mercenaria mercenaria from the Atlantic coast of the United States. The molecular characterization of its SSU rDNA region allowed obtaining a fragment of 1,796 bp showing 98% homology with M. mercenariae parasite. Phylogenetic analysis supported this identification as this parasite was clustered in the same clade as M. mercenariae from the United States and other M. mercenariae‐like sequences from the UK, with bootstrap value of 99%. The occurrence of M. mercenariae‐like parasites infecting molluscs outside the United States is confirmed.     

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Plasma estradiol-17 (E₂), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E₂ and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E₂ and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass.