桁拖网渔具分隔网片对虾类的分隔效率

利用分隔网片将桁拖网渔具网囊分为上下2层(虾囊和鱼囊)以实现虾类和鱼类渔获分离,对于改善桁拖网渔具作业性能、提高目标种类渔获质量以及实现选择性捕捞具有重要的意义.为分析不同网日尺寸(60mm、75mm和90mm)分隔网片对主要渔获种类的分隔效率,在吕泗渔场开展了2个航次的海上生产实验.利用分隔装置性能分析模型,对哈氏仿对虾(Parapenaeopsis hardwickii)和葛氏长臂虾(Palaemon gravier)的渔获数据进行拟合.结果表明,不同虾类接触分隔网片的概率没有显著性差异;虾类个体50%接触概率体长约为40mm,表明大多数进入网具的虾类都能接触分隔网片.随着分隔网片网目尺寸增大,50%选择体长也逐渐增大.因此,分隔网片的分隔效率随之提高.鱼囊和虾囊的网目尺寸相同,但结构有所差异.比较在不同网囊尺寸选择性假设条件下渔获数据的拟合结果后发现,鱼囊和虾囊具有相同选择性的假设更为合理;通过比较发现,分隔网片对虾类的尺寸选择性与相同网目尺寸的网囊选择性具有较大的差异.本研究还对产生这一差异的原因进行了探讨.     

广东大鹏湾虾拖网网囊网目选择性研究

虾拖网渔业是南海海域重要的作业方式之一,但尚未有关该渔业网目尺寸选择性的报道。为评价虾拖网渔具的渔获性能、合理利用虾类资源,在广东大鹏湾进行了网目选择性试验。试验采用双支架虾拖网作为试验网具,并使用套网法估算了网目内径为35、40和45 mm的网囊对主捕虾类的选择性。结果显示,虾类渔获中刀额新对虾、须赤虾、周氏新对虾和日本囊对虾渔获数量较多,比较以往资料,刀额新对虾和周氏新对虾优势体长、平均体长均有所下降,其它2种虾类平均体长未见明显变化;选择性分析结果显示,主捕虾类的L50随网目尺寸线性增大。根据试验结果并结合历史资料,笔者认为现阶段35 mm的网囊网目尺寸对于保护上述4种虾类资源是比较合适的。     

桁拖网渔具分隔网片对蟹类的分隔效果

传统捕虾桁拖网渔具网目小、渔获对象繁杂、选择性能差。使用网片式分隔装置、实现渔具的选择性捕捞是缓解这一问题的有效手段之一。2005年秋汛期间,在吕四渔场桁拖网渔业中开展了网目尺寸分别为60、75和90 mm分隔网片的海上生产试验,本文通过蟹类渔获为研究对象,分析分隔网片对其的分隔效果。试验结果显示,使用以个体接触分隔网片概率和网片对个体的留存率为基础的分隔效率模型,对蟹类甲宽分布数据的拟合发现,随着网目尺寸的增大,分隔网片对三疣梭子蟹(Portunus trituberculatus)、细点圆趾蟹(Ovalipes punctatus)的50%留存甲宽和留存率范围均有所增大;而上述2种蟹类个体接触不同网目尺寸分隔网片的概率则没有明显的一致性变化趋势。试验结果表明,蟹类在到达桁拖网网身部时具有一定的游泳能力且具有一定的游泳高度,也反映出使用向下倾斜的分隔网片在结构和装配上是比较合理的。     

桁拖网作业中鱼虾分隔的初步试验研究

1996年末,我们在浙江渔场进行了桁拖网作业的鱼虾分隔试验,如同我们过去对这类渔具所作的放大网目以释放鱼、虾幼体;伸长吊纲,提高网口以增加对三疣梭子蟹的捕捞产量(季星辉等,1984)一样,本次试验也是这类网具选择性研究的内容之一,目标是改善桁拖网的选择性。在提高这种作业经济效益的同时,充分兼顾保护渔业资源和海洋生态环境的社会效益。 试验工作是在现有多囊捕虾桁拖网上,选择其中一个囊网,用一片目大为100mm的网片进行分隔,使鱼进入原囊网下方增设的盛鱼囊袋,而虾因其遇惊会弹跳的习性,穿过分隔网片进入原囊网。实践证明,分隔的效果是明显的,虾的分隔率达到55～68％,鱼的分隔率达到95％。现有的试验是初步的,我们将创造条件继续下去。     

硇洲岛周围水域虾拖网虾渔获组成

根据2005年渔业资源监测船生产调查资料和现场取样数据,运用数理统计方法分析了虾拖网虾渔获组成、生物学特征以及月份变化等。结果表明:硇洲岛周围水域虾拖网捕获的虾类主要是沿岸性底栖虾类,渔获中优质虾种少,幼虾比例高,墨吉明对虾、日本囊对虾、哈氏仿对虾是虾拖网渔业的主捕虾种,虾渔获组成存在明显的月份变化。为合理利用虾类资源和促进虾渔业可持续发展,应根据虾类特性开发适宜广东沿海虾拖网的选择性捕捞技术,减少幼虾兼捕量。     

再谈网囊改革

笔者曾于《中国水产》1993年第五期发表过《使用方形网目网片有利于保护资源》文章,现就有关问题进行补充。 拖网网囊的主要作用在于聚积渔获物,网囊的网目形状、网目尺寸及网囊本身的形状,对鱼类具有很大的捕捞选择性。它们不但会影响拖网的渔获量,而且会影响网囊释放幼鱼和保护鱼类资源的效果。可见,拖网网囊的设计无疑是拖网设计的一个重要环节,在新设计拖网时必须兼顾考虑上述关系。 (一) 30年代以来,世界上许多渔业发达的国家都把规定拖网网囊网目尺寸作为拖网渔业管理的重要措施之一,一直在进行拖网网囊网目选择性试验和研究。早     

鱼虾分离器对虾拖网渔获的影响

在虾拖网渔业的混合渔获中鱼的平均分布率高达81％，不仅鱼类资源有巨大浪费，而且对鱼类资源的繁殖也产生严重影响。为了减少幼鱼副渔获，经常采用网囊的网目选择性。但是，早期的研究证明，许多变量影响拖网和其他移动性渔具中鱼类的尺寸选择。要克服的最大问题之一就是网囊渔获增加后有效网目张开减小。     

南海区双船大网目拖网网囊网目对带鱼的选择性

大网目拖网是我国海洋渔业捕捞带鱼的重要渔具。为提高拖网网囊选择性, 本研究在南海区采用套网法对双船大网目拖网 4 组网目尺寸(30、35、40 和 45 mm)的网囊进行实验, 在双重拔靴法的架构下, 拟合选择性参数、比较选择性的差异、估算捕捞方式指标。结果表明, 随着网目尺寸的增大, 网囊对带鱼的 50%选择体长增大, 对带鱼幼鱼个体的选择率显著下降, 带鱼幼鱼的渔获比例也呈下降趋势。结合带鱼最小可捕规格(23.0 cm)管理规定, 结果还表明网目尺寸为 40 mm 的网囊选择性较差, 其对带鱼的抛弃率大于 93%。为了进一步提高带鱼拖网渔业的资源养护水平, 还需要开展更多的选择性实验。     

多囊桁拖网选择性研究中对照网囊网目尺寸的选择

对比作业法是拖网渔具选择性研究中重要的试验方法之一,而对比试验中对照网囊网目的选择至关重要。研究利用多囊桁拖网不同网目尺寸(20、30、35和40mm)网囊捕获的哈氏仿对虾、小黄鱼和棘头梅童鱼渔获体长分布数据,应用几何相似原理,分析过滤性渔具的选择性,并按不同假设条件下(假设1:符合几何相似原理对渔获具有一定的选择性;假设2:对渔获种类没有选择性),探讨如何合理选择对照网囊网目尺寸。结果表明,对照网网囊网目尺寸为20mm,在上述2种假设下,对选择性模型的拟合、简化及对其它网囊的选择性参数没有显著的影响;在考虑了桁拖网渔具各网囊具有相同相对作业强度,以及桁拖网渔具与普通拖网渔具选择性分析方法上的差异等因素后,认为在此项研究中,将小于20mm网目尺寸的网囊视为没有选择性的对照网囊是可行的。     

关于解决鱼虾混获问题的设想

<正> 目前我国渔民捕虾用的渔具有拖网、流刺网、张网和敷网,其中以拖网的捕捞效率为最高。但是,在虾拖网渔业中,鱼虾混获却成为我国虾拖网渔业者长期以来迫切期望解决的一个重大难题。一般来说,虾拖网的网目尺寸比鱼拖网的网目尺寸小得多,所以这种虾拖网在捕虾作业过程中不可避免要混     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Plasma estradiol-17 (E₂), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E₂ and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E₂ and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass.     

Cardiac,ventilatory and metabolic responses of two ecologically dissimilar species of fish to waterborne cyanide

Changes in heart rate, ventilatory activity and oxygen consumption were determined in trout (Salmo gairdneri) and brown bullhead catfish (Ictalurus nebulosus) during exposure to a steadily increasing concentration of waterborne cyanide selected to produce death in 8–9 hours for each species. The lethal cyanide concentration for the bullheads was an order of magnitude higher than for trout. Trout developed an immediate and gradually increasing bradycardia throughout the exposure period. Cyanide produced tachycardia in the bullhead followed by a gradual onset of bradycardia as the concentration of cyanide was raised. Pericardial injection of atropine (a muscarinic cholinergic antagonist) indicated that bradycardia in the trout was due initially to increased vagal tone but later due to the direct effect of cyanide on the heart. Hyperventilation in the trout persisted throughout the exposure period, although the rate and amplitude fluctuated and was variable between individual fish. During the last hour of exposure (highest cyanide concentration), ventilation was characterized by rapid, shallow breaths followed by a sudden respiratory arrest. The bullheads exhibited hyperventilation during the first 3 hours of exposure followed by a gradual, linear drop in ventilation rate and amplitude until death occurred. Cardiac and ventilatory responses in both species were attributed to stimulation of central and peripheral chemoreceptors by cyanide. Evidence is presented which suggests the initial response in the bullheads was due, at least in part, to gustatory stimulation by the cyanide. Oxygen consumption of the trout remained above pre-exposure levels for the majority of the test period. Oxygen consumption in the bullhead paralleled the changes in heart and ventilatory rates. Whole-body lactate levels of fingerlings of both species during cyanide exposure were measured to estimate the extent of anaerobiosis. Whole-body lactate levels were much greater in the bullheads than the trout, indicating a higher capacity for anaerobiosis, possibly due to a greater fuel supply. Overall, the trout responded to cyanide in a manner similar to that produced by environmental hypoxia whereas the bullheads experienced a gustatory stimulus which masked the hypoxia-like response.     

An overview of stress physiology of fish transport: changes in water quality as a function of transport duration

This study brings an integrated analysis about the relationship between water deterioration and its physiological consequences in live fish transport. The analysis was focused on the transport water and its deterioration, and physiological challenges imposed on the fish. Usual commercial handling procedures employed to mitigate fish stress during transport were discussed. Future topics of research for the establishment of safer fish transport protocols were proposed. Transport was classified into short (≤8 h) or long transport (>8 h). The main issue in short transports should be the prevention of water pH reduction, while in long transports it is the increase in ammonia. Plasma cortisol is the most employed marker for stress and is acutely elevated upon short episodes of transport, but remains elevated even in long‐transport events. Plasma glucose is perhaps a better marker for handling stress. Plasma lactate, pH, osmolality CO₂ and ions should be more often evaluated. Plasma Na⁺ and Cl^‐ are very useful markers of acidosis, due to their respective exchange for H⁺ and , for acid–base regulation. The establishment of species‐specific transport protocols should be preceded by such combined analyses of water and physiological parameters.     

Are hatchery‐reared abalone naïve of predators? Comparing the behaviours of wild and hatchery‐reared northern abalone,Haliotis kamtschatkana (Jonas, 1845)

Abalone populations have declined worldwide, generating interest in enhancement using hatchery‐reared individuals. In many cases, such restoration efforts have met with limited success due to high predator‐induced mortality rates. Furthermore, the mortality rates of outplanted hatchery abalone are often considerably higher than for wild individuals. This study uses northern abalone (Haliotis kamtschatkana) as a case study to determine whether hatchery‐reared abalone behave differently than their wild counterparts. In the field, outplanted hatchery‐reared abalone were significantly less responsive than wild abalone, in terms of number of abalone responding and intensity of response, to nearby movement and to physical contact with an inert probe. Also, when encountering a cue to which all abalone responded (a seastar predator), hatchery‐reared individuals remained subdued. Anti‐predator behavioural deficits in hatchery‐reared abalone were more pronounced in 4‐year‐old individuals than in 1‐year‐old individuals, suggesting an influence of either age or amount of time spent in the hatchery environment. These behavioural differences are expected to increase the vulnerability of hatchery‐reared abalone to predators, and are likely a major cause of their elevated predator‐induced mortality when outplanted.     

Effects of cadmium on the kinetics of calcium uptake in developing tilapia larvae, Oreochromis mossambicus

The toxic effects of Cd²⁺ on Ca²⁺ influx kinetics in developing tilapia (Oreochromis mossambicus) larvae were evaluated. Addition of 20 µg l^-1 of Cd²⁺ to the environment of 0 and 3 day-old larvae competitively inhibited the Ca²⁺ uptake within 4h resulting in a great increase in K_m values for Ca²⁺ influx (19.3 and 17.4 fold, respectively) as compared with their respective controls. Consequently, the actual Ca²⁺ influx of larvae in solutions of 0.2 mM Ca²⁺ are suppressed by 32–45%. Also, 3 day-old larvae were more sensitive to internally accumulated Cd²⁺ than 0 day-old larvae. Although the Ca²⁺ influx in 0 and 3 day-old larvae may be restored to the levels of their respective controls with 24h of being transferred to a 20 µg l^-1 Cd²⁺ solution, total body Ca²⁺ content was significantly reduced in 3 day-old larvae. Increased Ca²⁺ uptake efficiency ensures sufficient Ca²⁺ for normal growth. However, rapid increase in Ca²⁺ influx after hatching also leads to higher Cd²⁺ uptake. Exposure to Cd²⁺ will lead to a drop in body Ca²⁺ content resulting in retardation of larval growth. Therefore, we conclude that if Ca²⁺ uptake is interfered with at this critical stage of development, larvae will not be able to maintain normal levels of body Ca²⁺ and will show signs of Cd²⁺ poisoning.     

Migratory dynamics of stream-spawning longnose gar (Lepisosteus osseus)

Abstract– Literature evidence suggests that lake-dwelling longnose gar (Lepisosteus osseus) enter tributary streams to spawn, Until the present study, the dynamics of this breeding migration had never been investigated quantitatively. During the summers of 1991 and 1992, longnose gar were captured as they entered Weaubleau Creek, Missouri, a tributary of Harry S. Truman Reservoir. The in-stream spawning migration began in early April and ended in late May, and was positively correlated with stream flow and water level, and negatively correlated with water temperature. In-stream residence times ranged from 15 to 94 days, with males exhibiting longer residence times than females. Once in-stream, longnose gar travelled as far as 10 km upstream and occupied certain pools at greater relative frequencies. Although the reason for this preferential utilization is not completely understood, it may relate to pool depth and riffle proximity. Longnose gar disperse from the spawning stream great distances, with gar captured in Weaubleau Creek being recaptured up to 48 km away. This information should provide fisheries biologists the means to consider the reproductive ecology of this species in their conservation and management decisions.     

Nutritional regulation of hepatocyte fatty acid desaturation and polyunsaturated fatty acid composition in zebrafish (Danio rerio) and tilapia (Oreochromis niloticus)

The desaturation and elongation of [1-¹⁴C]18:3n-3 was investigated in hepatocytes of the tropical warm freshwater species, zebrafish (Danio rerio) and Nile tilapia (Oreochromis niloticus). The hepatocyte fatty acid desaturation/elongation pathway was assayed before and after the fish were fed two experimental diets, a control diet containing fish oil (FO) and a diet containing vegetable oil (VO; a blend of olive, linseed and high oleic acid sunflower oils) for 10 weeks. The VO diet was formulated to provide 1% each of 18:2n-6 and 18:3n-3, and so satisfy the possible EFA requirements of zebrafish and tilapia. At the end of the dietary trial, the lipid and fatty acid composition was determined in whole zebrafish, and liver, white muscle and brain of tilapia. Both zebrafish and tilapia expressed a hepatocyte fatty acid desaturation/elongation pattern consistent with them being freshwater and planktonivorous fish. The data also showed that hepatic fatty acid desaturation/elongation was nutritionally regulated with the activities being higher in fish fed the VO diet compared to fish fed the FO diet. In zebrafish, the main effect of the VO diet was increased fatty acid Δ6 desaturase activity resulting in the production of significantly more 18:4n-3 compared to fish fed the FO diet. In tilapia, all activities in the pathway were greater in fish fed the VO diet resulting in increased amounts of all fatty acids in the pathway, but primarily eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3). However, the fatty acid compositional data indicated that despite increased activity, desaturation of 18:3n-3 was insufficient to maintain tissue proportions of EPA and DHA in fish fed the VO diet at the same level as in fish fed the FO diet. Practically, these results indicate that manipulation of tilapia diets in commercial culture in response to the declining global fish oil market would have important consequences for fish fatty acid composition and the health of consumers. Scientifically, zebrafish and tilapia, both the subject of active genome mapping projects, could be useful models for studies of lipid and fatty acid metabolism at a molecular biological and genetic level. This revised version was published online in August 2006 with corrections to the Cover Date.     

Production of diatom–bacteria biofilm isolated from Seriola lalandi cultures for aquaculture application

Controlled incorporation of selected microalgae and bacteria in aquaculture systems can be beneficial because they can act as microbiological control. That is why the characteristics of biofilm generated naturally in Seriola lalandi culture cages were analysed, their potential benefit to the growth of larvae was studied, and their controlled use for improving the larval viability and as a vector to improve incorporation of previously studied probiotic bacteria was tested. According to biodiversity results, these biofilms are composed of a diatom–bacteria mix showing a decrease in biodiversity in laboratory culture conditions being dominated by Navicula phyllepta and bacteria of the family Rhodobacteraceae. This can be produced on mesh substrates incorporated in bioreactors with rapid growth rate and adhesiveness. Preliminary results from the addition of substrates with this specific biofilm in larvae culture systems showed that it is consumed by the larvae without negative effects, while positive effects on the viability of larvae in combination with probiotics were observed. Considering preliminary results, the addition of these specific substrates with diatom–bacteria biofilms could be a good improvement for aquaculture systems and together with the use of probiotics can contribute to maintaining a stable and controlled system improving the viability of the larval fish culture in its early stages.