Lion's paw scallop (Nodipecten subnodosus, Sowerby 1835) aquaculture in Bahía Magdalena, Mexico: effects of population density and season on juvenile growth and mortality

This study examined the effects of density and growing season on growth and survival of juvenile lion's paw scallops (Nodipecten subnodosus) in Estero San Buto, a mangrove channel in Bahía Magdalena, Mexico. Scallops were kept in plastic mesh bags in oyster trays at three population densities (500, 1000 and 2000 organisms per tray, or low medium and high density respectively) over a period of 2 months from October to December 2001 and from February to April 2002. Growth (shell height increment) was measured every 7–12 days and mortality was evaluated at the end of the experiment (dead shell count). Overall growth was fast (0.24–0.38 mm day^?1) in comparison with other pectinids. Significant differences were found for both, density and season, with faster growth occurring at lower densities and during the fall season when the water temperature was higher. Mortality was low (0.5–3.0% 60 day^?1) except for the high‐density treatment in the fall (44% 60 day^?1). Crowding together with high water temperatures and increased metabolic oxygen demand of the scallops and possibly competition for food were the probable reasons for this high mortality. Overall, mortality was lower in the spring, when temperatures were lower, O₂ values were higher and food was more abundant.     

Post-transport recovery of cultured scallop (<Emphasis Type="Italic">Pecten maximus</Emphasis>) spat,juveniles and adults

High mortality associated with transport operations in scallop culture has been a major problem faced by European farmers. Simulated transport with Pecten maximus L. spat <2 mm, spat 15–30 mm, juveniles 30–50 mm and adults >100 mm were carried out in Spain, Ireland and Norway. Different time and temperature combinations were studied in order to maximise post-transport survival and establish best practices. Out-of-water transport could result in 100% survival if conditions were right, but the response to emersion stress depended on size, season and location. Post-transport recovery decreased with emersion time and was strongly influenced by temperature. Air exposure was tolerated for a longer time by adult scallops than spat and juveniles, but the results differed among trials in the different countries. The maximum emersion time that gave post-transport survival ≥80% was 12 h for the smallest spat, 18 h for larger spat and 24 h for juvenile and adult scallops. Adults were less affected by transport temperatures that deviated from ambient seawater temperature than spat and juveniles. In general post-transport recovery was high when sea temperature was <10°C, but during warm-water seasons special care should be taken to avoid stressful and lethal transport conditions. A transport temperature <12°C was recommended, though not more than 10°C below ambient culture temperature. A maximum transport time of 9 h was suggested for spat and juveniles to attain post-transport survival close to 100%, but 12–24 h was feasible during the cold-water season or at favourable transport temperatures.     

Effect of air exposure on scallop behaviour, and the implications for subsequent survival in culture

Scallop spat produced for continued culture normally requiretransportation between sites, and the associated stresses may causemortalities. In the present experiment, scallops of 40–55 mm shell heightwere emersed in air for time intervals up to 24 h and their behaviourexamined once re-immersed. Scallops were placed upside-down and thenumber of movements and the cumulative numbers righting in 5 min timeblocks were recorded. The greatest frequency for all behaviouralresponses was found at 15–17 °C in August. Responses were reducedin November and June (9–11 °C) and least at 5 °C in January.All effects of treatment, temperatures and season, and increases in airexposure were significantly different. Following the treatments, mortalitiesafter 10 days in culture was about 10–30% for scallops emersed for 18 and24 h in August and June. Scallops did not show significantly differentbehaviour whether they were emersed upright or inverted. However,scallops emersed at 15 °C had fewer responses than scallops held at <10 °C, so chilling during transport may prolong scallop vitality. Theresults suggest that air exposure >12 h should be avoided. At temperatures>9 °C, behavioural responses may be a simple and effective method toassess vitality which can assist in the management of scallop culture.     

Effect of immersion time of cultch on spatfall of the scallop Argopecten purpuratus (Lamarck 1819) in the Marine Reserve at La Riconada, Antofagasta, Chile

A pilot program to evaluate the feasibility of collecting juvenile (seed) scallops using Japanese technology was carried out from October 2001 to April 2002 in the Marine Reserve at La Rinconada, Antofagasta, Chile. The continuous presence of larvae in waters in the reserve allowed for collection of 400–15,340 spat per collector and showed the high reproductive capability of local scallops and the relationship between numbers of larvae in the water and amount of spatfall. Highest rates of settlement were proportional to high numbers of umbone larvae in the water at >5000 larvae m⁻³ when collectors were first immersed. Absence of a relationship between larval numbers and postlarval settlement with trophic factors such as chlorophyll and particulate organic matter in the water suggests that productivity was not limited by these factors. Harvest of spat from collectors immersed for four and five months had recovery efficiencies of 5.5% and 59.4% compared with settlement in collectors after immersion for one month. Cohort analyses of recovered spat suggested that settlement occurred over the lengthy immersion period and was due to the continuous presence of larvae in the water. The loss of a high percentage of spat over the 4–5 immersion period compared with settlement in the first month can be attributed to intraspecific competition of spat density and growth as well as clogging of the collectors by sediment and marine fouling that impeded a flow of water through the nets.     

Growth of the European flat oyster in the Mediterranean Sea (Murcia, SE Spain)

Growth of the natural European flat oyster (Ostrea edulis L.) spat from the Mar Menor (Murcia, Spain) was studied in the Mediterranean Sea over 18 months. The oysters were cultured in two types of containers, stackable plastic Galician trays and plastic mesh pots, deployed at a depth of 15 m hung from a long-line. Two size classes of spat were used, Class I spat (initially 53 mm in size and 19 g in weight) and class II spat (initially 31 mm, 4 g). The best results were observed in the class I oysters grown in the trays, which yielded a substantial biomass (24.77 kg m^–2), the survival rate was 69% and commercial size (60 mm) was attained by all the oysters within 9 months of the start of the experiment. The observed growth of the class II oysters was poor, attaining a mean of not more than 50 mm and 18 g; the survival rate ranged between 25–74% and only between 8–13% of the class II spat attained commercial size.     

Status of a commercial sea scallop enhancement project in Iles-de-la-Madeleine (Quebec, Canada)

The main objective of the REPERE (REcherche sur le Petoncle a des fins d'Elevage et de REpeuplement) research programme, initiated in the Iles-de-la-Madeleine in 1991, was to enhance natural sea scallop (Placopecten magellanicus) populations which were greatly reduced by overfishing. Sea bed seeding was chosen as the best approach to achieve this goal. In 1992, after two years of scientific research work, mainly centred on producing a supply of juveniles and developing intermediate culture methods, the Scallop Fishermen's Association, supported by governmental financing, initiated a commercial seeding project. Two thousand collectors were set out in open waters suitable for spat settlement. A collecting rate of 780 spat per collector produced a catch of 1.5 million spat. Technical problems led to significant losses of juveniles. Intermediate culture in pearl nets in a lagoon, between July and October 1993, enhanced growth noticeably. At an age of 1 y, average shell height was 32 mm. In the fall of 1993, 30 000 young scallops were seeded on a natural bed closed to fishing. Ten thousand collectors were set out in the fall of 1993. Collecting success was 1145 spat per collector and spat losses were reduced by technical improvements. In November 1994, 1.5 million scallops were seeded on a sea bed closed to the fishery. Scallops from the 1994 seeding could reach market size (90 mm) by 1997.     

Strategies for enhancement of natural bay scallop, Argopecten irradians irradians, populations; A case study in the Niantic River estuary, Connecticut, USA

We investigated strategies to enhance populations of bay scallops,Argopecten irradians irradians (Lamarck, 1819), in a presumablyrecruitment-limited natural habitat. At present, the Niantic Riverestuary supports only a minor bay scallop population that is harvestedrecreationally. Three enhancement strategies were evaluated; (1)collection and redistribution of natural spatfall, (2) introduction andover-wintering of hatchery-reared stock into natural habitat to providenew spawning stock, and (3) over-wintering of hatchery-reared stock insuspension culture for creation of mobile spawner sanctuaries. Anassessment of natural bay scallop recruitment in the Niantic Riverconducted in 1997 indicated that few spat were found, they were widelydispersed within the river, and peak spawning occurred in late July1997. Direct re-seeding was evaluated as an enhancement measure byplanting hatchery-reared scallops ( 38 mm shell height) insmall-scale, 100-m² plots at different times and densities.Time of planting and the inferred predation intensity were major factorsaffecting survival; whereas, planting density had no significant effect.Approximately 9,000 scallops (35–45 mm shell height), broadcastwithin an eelgrass bed in November 1997, had high over-winter survivaland underwent gametogenesis and spawning during 1998. Of 26,000 bayscallops ( 45 mm shell height) over-wintered in suspensionculture from 1998–1999, approximately 60–80%survived, and these scallops spawned in mobile sanctuaries, during thesummer of 1999. There is good potential for using aquacultural methodsfor enhancement of bay scallop populations when natural recruitment ispoor and habitat and environmental conditions are not limiting.     

Effects of air emersion on survival and growth of hatchery rearedgreat scallop spat

Scallop spat production normally requires transfer between growthsystems. Simulated transport experiments were carried out in April, June,December and February to evaluate effects of transport time on greatscallop (Pecten maximus) spat growth and survival. The spat (1.7–1.8 mm in shell-height and 21–25 µg ash free dry weight [AFDW]) wereheld in moist coffee filters at a temperature of 10 °C for up to 24 h,before being replaced into sieves in rearing tanks at 15 °C. The studyshowed that by increasing air emersion time, survival and growthdecreased. No significant difference in the results between 0 and 4 h of airemersion was found, while the effects after 12 and 24 h differed betweenspat groups. Survival and growth rates showed seasonal differences. Meansurvival was 35–71% in April and 77–99% from June to February. In Junemean growth rates attained were 115–128 µm shell-height and 15–18µg AFDW per day compared with 49–69 µm and 3.8–7.0 µgper day for the other spat groups. Great scallop spat may survive atransfer time of 24 h, but transportation for longer than 12 h is notrecommended if subsequent high survival and growth rates are to beensured.     

Mortality of scallop spat in cultivation, infested with tube dwellingbristle worms, Polydora sp.

Between 1995 and 1997, a traditional Norwegian oyster poll with an attachednursery was used for the production of scallop (Pecten maximus) spat.During summer 1997, four batches of 2 mm scallop spat were placed in thenursery. In June, hydroids (Obelia) caused problems with fouling andaccumulation of sediment in the nursery. Thereafter, the scallop spat wereheavily infested with tube dwelling polychaets (Polydora sp.). Thepolychaets were both introduced with poll water and reproduced inside thenursery. Infested spat suffered high mortalities. Although the spat could have been affected by the hydroids, as well as shortage of food, Polydorainfestations were considered the main cause of the mortalities. Dead andinfested spat were continuously sorted out and discarded, but the infestationproblems persisted. As a result, the scallop spat did not represent anycommercial value for the company, and in September, the nursery wasemptied. In total, approximately one million spat – representing onethird of Norway's intensive scallop spat production in 1997, was lost.     

Spat Collection and Growth to Commercial Size of the Tehuelche Scallop Aequipecten tehuelchus (D'Orb.) in the San Matias Gulf, Patagonia, Argentina

Studies on larval settlement and growth of the commercial scallop Aequipecten tehuelchus were carried out over a 2-yr period in San Matias Gulf, Argentina. Gonadal index was used to indicate spawning and spat collectors were deployed when spawning began. Artificial spat collectors (mono-filament bags filled with thin shrub branches) were placed at a depth of 25 m in two areas of the Gulf during two seasons (1989–1990 and 1991–1992). Maximum settlement occurred by mid-February during the first season and by mid-January in the second season. Larger numbers of spat were found in collectors placed near the sea bottom. After settlement, spat were removed and placed in cages for growout. Scallops reached the average commercial size (60 mm shell height) 16 mo later. Mortality rate during the entire period was 10%.     

Influence of environmental factors,season and size at deployment on growth and retrieval of postlarval lion's paw scallop Nodipecten nodosus (Linnaeus, 1758) from a subtropical environment

The effect of seasonal variation of environmental factors on daily shell growth rates (DGR) of postlarval Nodipecten nodosus was studied at the southern distribution limit of the species in Santa Catarina State, Brazil. Five deployments of hatchery produced postlarvae (initial shell height 0.5 mm) in the sea-based nursery were carried out from August 2000 to September 2001, and DGR and percent retrievals were recorded. Chlorophyll-a, seston, salinity, dissolved oxygen and turbidity were measured weekly, and temperature was recorded hourly. Additionally, DGR and retrievals were compared for postlarvae maintained simultaneously in the sea- and land-based nurseries (initial shell height 0.5 mm), and also for post-larvae deployed in the sea-based nursery at different initial sizes (0.29–1.1 mm). Mean DGR was significantly lower in late winter–early spring 2000 (0.045 mm day⁻¹), intermediate in late winter–early spring 2001 (0.078 mm day⁻¹) and significantly higher in the other seasons (late spring–early summer, 0.152 mm day⁻¹; late summer–early autumn, 0.149 mm day⁻¹ and late–autumn early winter, 0.130 mm day⁻¹). Temperature was the best predictor of growth, which was least at temperatures below 20 °C. Growth rate was also minimal during a period of low salinity and high turbidity. Mean DGR was significantly higher in postlarvae deployed in the sea-based nursery than in those maintained in the land-based nursery. Loss of postlarvae in the sea-based nursery was initially higher in collectors transferred earlier to the sea (ca. 2–3 weeks post-set; shell height 0.5–0.8 mm), but percent retrievals were similar after postlarvae deployed to the sea ca. 4–5 weeks post-set (shell height 1.1 mm) were retrieved simultaneously with those deployed earlier. At retrieval, postlarvae deployed approximately 2 weeks post-set were larger than those deployed subsequently, but spat deployed 1 week post-set attained a similar size to those deployed 2 weeks post-set. A strategy to deploy postlarvae in the sea-based nursery at a size circa 0.5 mm is proposed as more advantageous than keeping them longer in land-based facilities. In southern Brazil, there is a wide window of opportunity to deploy post-larval scallops in the sea-based nursery in which growth is maximized, except when water temperatures drop below 20 °C.     

Effects of water depth on survival and growth of <Emphasis Type="Italic">Argopecten purpuratus</Emphasis> (Lamarck, 1819) spat in northern Chile

Collection and suspended culture of Argopecten purpuratus spat in Japanese-type collectors was undertaken in the Rinconada Marine Reserve (Antofagasta, Chile) to determine growth variations between surface and bottom waters. Scallop spat was collected at 16-m depth and grown at 1- and 16-m depths. An initial settlement of ca. 13,000 post-larvae per bottom collector (2 cohorts) was observed on 3 February 2001. Two new cohorts settled in bottom collectors on 3 March, while no spat settlement occurred in surface collectors. The four cohorts exhibited substantial and different mortality rates over the study period, depending on the cohort and on-growing depth. The first and second cohorts exhibited mortality rates of 80.3 and 53.1% in bottom collectors, respectively, versus 29 and 43.6% in surface structures. The third and fourth cohorts exhibited mortality rates of 21.4 and 59.7% in bottom collectors, respectively. Mean shell height of scallop spat after 88 days of culture was 6.46 mm for the first cohort and 4.37 mm for the second cohort in bottom collectors, versus 10.33 and 7.31 mm in surface collectors, respectively. Mean post-larval growth rate in bottom collectors (86 μm day⁻¹) was significantly lower than growth rate in surface collectors (146 μm day⁻¹). Factors explaining the depth-related mortality and growth rates are discussed to improve scallop culture in the Reserve. It was concluded from the results that culture improvement in Antofagasta Bay would require collecting spat in bottom waters and raising it near the surface during initial cultivation stages.     

Survivorship and growth rates of hatchery-reared sea urchins

The survivorship and growth rates of juvenile, hatchery rearedPsammechinus miliaris (Gmelin) were observed for seaurchins fed different diets, and maintained at varying stocking densities andlocations (tank or sea-based). In addition survivorship and growth of secondgeneration hatchery stock was also recorded. Somatic growth rates of juvenileurchins (initial test diameter 6.5 mm SD 1.26) were measured over9months. A high lipid, high protein diet supported higher somatic growth ofurchins in this size range. Higher stocking densities (4 individualsl^–1 as opposed to 2 individuals l^–1)adversely affected growth as did location (sea-based urchins being smaller thantank based) although survivorship in all treatments and in both first andsecondgenerations was exceptionally high. Further research is required to designdietspromoting somatic rather than gonadal growth in urchins below market size.     

Comparison of early life history stages of the bay scallop, Argopecten irradians: Effects of microalgal diets on growth and biochemical composition

The culture of bay scallops, Argopecten irradians, is limited by a reliable and affordable supply of spat and the ability to ensure that animals attain market size within a single growing season. The main goals of our study were thus: (1) to develop growth-optimizing algal diets for implementation in hatcheries, and (2) to identify and compare bay scallop postlarval and juvenile dietary requirements, especially of lipids and fatty acids, which if met may enhance production. Nutritional needs of postlarval bay scallops (present study) are compared with those of sea scallops, Placopecten magellanicus, offered the same diets in a previous companion study. To this end, postlarval (initial shell height, SH = 240 μm) and juvenile (initial SH = 10 mm) bay scallops were offered 6–7 microalgal diet combinations at 20 °C, for 3 weeks. A similar growth ranking among diets was observed between the two developmental stages. A combination diet of Pavlova sp. (CCMP 459) and Chaetoceros muelleri was far superior to any other diet tested, yielding growth rates of 58 and 357 μm day^− 1 which were 65% and 25% higher than the next highest performing diet of Tetraselmis striata/C. muelleri in postlarvae and juveniles, respectively. The T. striata/C. muelleri diet, which is limited in the n-3 fatty acid docosahexaenoic acid (DHA), yielded very poor growth of sea scallop postlarvae in a prior study, indicating that bay scallops may have less stringent requirements for DHA than sea scallops. The Pav 459/C. muelleri diet, which also supported the highest growth of sea scallop postlarvae, is characterized by elevated levels of the n-6 fatty acids, arachidonic (AA) in C. muelleri and 4,7,10,13,16-docosapentaenoic (DPA) in Pav 459. The two diets deficient in AA and n-6 DPA, Pavlova lutheri/Thalassiosira weissflogii and P. lutheri/Fragilaria familica, yielded the lowest growth rates in both bay scallop postlarvae and juveniles. Tissue enrichment of these two fatty acids relative to the diet, as well as overall enrichment in ∑n-6 fatty acids was observed across developmental stages and dietary treatments. A similar pattern has previously been observed in sea scallop postlarvae, suggesting a dietary requirement for n-6 fatty acids in pectinids that has often been overlooked in the past.     

A pump-pot nursery system for rearing juvenile New Zealand dredge oysters Tiostrea lutaria

A wave-operated nursery system was developed for growing hatchery-produced spat of the New Zealand dredge oyster Tiostrea lutaria (Hutton) through to a size (≈ 20 mm) suitable for on-growing in conventional oyster farming equipment. The pump-pot enabled cultch-free dredge oyster spat to be transferred from hatchery upwellers at a small size (> 2 mm), thus avoiding the need to supply them with cultured algae to maintain a high growth rate. The wave-activated pumping action of the pot maintained a sufficient flow of water through the pot to achieve rapid growth of small spat held at high densities.     

Nursery growth, survival and chemical composition of great scallop Pecten maximus (L.) spat from different larval settlement groups

Scallop Pecten maximus spat (1.3–2.1 mm shell height) from different settlement groups were transferred from hatchery to land‐based nursery at different ages and sizes. Chemical content, growth and survival were compared at transfer time and after 1 and 8 weeks of nursery growth. Growth was lowest and mortality highest in the first week after transfer. Mean shell height growth was 21.5–71.4 μm day^?1 and ash‐free dry weight (AFDW) growth ?2.7 to 10.3 μg day^?1. Spat from the first settlement group attained a larger size and weight than spat from larvae settled 3 days later, but had a lower daily growth rate (%). Keeping the late‐settled spat a longer time in the hatchery to reach a bigger size before transfer seemed not to improve subsequent nursery growth. Survival showed a large variation with mean survival ranging from 32% to 74%. A substantial reduction in lipid content was found after transfer to the nursery. Sterol content at transfer was the only lipid class correlating with survival in the nursery. Based on the results, it is justified that spat groups of different settlement age are included in production of 15‐mm great scallop spat if they are transferred from the hatchery at the same age.     

Growth and mortality of the king scallop grown in suspended culture in Malaga, Southern Spain

Growth and mortality of the king scallop, Pecten maximus, werecompared when grown in cages and by ear hanging in suspended culturein Fuengirola, Malaga, in southern Spain. Seed (juveniles) used in theexperiment was collected in September 1997 that had settled on collectorsin April-June, of that year. Culture in suspended cages began in January1998 when the seed measured 42.7 (3.3) mm shell height and ended inFebruary 1999. Significantly faster growth was found at a minimum culturedensity (16 scallops/cage) than at two other densities (24 and 36scallops/cage). Depth (1, 5 and 10 m from the bottom) influenced growth,poorest growth occurred closest to the bottom. Under optimum growingconditions, 16 scallops/cage suspended 10 m from the bottom, scallops grewto 10 cm shell length (legal size) by February 1999.In ear hanging culture, ropes were moored in April (51.3 (4.5) mm),June (58.2 (4.5) mm) and November 1998 (64.3 (4.9) mm).Initially, rapid shell growth was observed in all three cultures.Subsequently, the shells became covered with barnacles, Balanus sp.,that possibly caused total mortality of the April culture and led to highmortalities in the two other cultures.     

Utility of high-density plantings in bay scallop, Argopecten irradians irradians, restoration

Two different methods of establishing high-density spawner sanctuaries for bay scallop (Argopecten irradians irradians) restoration were evaluated over 2 years at a site in Northwest Harbor, East Hampton, New York, USA. Hatchery-reared scallops, which had been overwintered at nearby sites, were free-planted directly to the bottom in late March/early April at an initial target density of 94?C128 scallops/m². In addition, scallops were stocked in off-bottom culture units consisting of three vertically stacked 15-mm mesh ADPI^® bags at densities of 50, 100, or 200 scallops/bag (=117, 234, or 468 scallops/m²), respectively. Survival of scallops differed significantly by year, planting method, and scallop source. Survival of free-planted scallops was generally lower than caged scallops. Better survival of free-planted scallops in 2005 versus 2006 likely reflected the presence of luxuriant eelgrass beds in 2005, which were absent in 2006. Survival of scallops in ADPI bags was not appreciably related to stocking density. Shell growth was highest for free-planted scallops; in cages, growth was somewhat better at 50 versus 200 scallops/bag. Wet weights of epibionts were significantly higher in caged versus free-planted scallops. Reproductive condition of scallops stocked at 50/bag was usually higher than at 200/bag. Both free-planting and off-bottom systems yielded high densities of adult bay scallops at the time of spawning, which ensures a higher probability of successful fertilization of spawned eggs and thus a greater potential for success of restoration efforts.     

Reproduction of hatchery-reared and transplanted wild bay scallops, Argopecten irradians irradians, relative to natural populations

Efforts to restore bay scallop populations in the United States throughtransplantation of wild stock and reseeding of hatchery-reared individuals haveincreased due to declines in natural populations, yet little is known of thecomparative spawning patterns and relative reproductive investment of thesedifferent groups. In this study, spawning patterns of wild scallops from asource population in Northwest Harbor, New York and of scallops transplanted toa distant site in the same embayment (Flanders Bay) were similar.Hatchery-reared scallops held in pearl nets in Hallock Bay, New York showed thesame temporal spawning pattern and level of reproductive investment as scallopsfrom adjacent wild populations and wild scallops held in pearl nets. We suggestthat hatchery-reared scallops may be equally valuable as potential broodstockfor reseeding operations as wild scallops of the same size, provided thatappropriate conditioning and rearing practices are maintained. Both wild andhatchery-reared scallops may be transplanted a short period (i.e. 2–4weeks) prior to expected spawning to provide a source of potential broodstockinareas where natural population densities are low.     

健跳港上游湾区牡蛎潮间带分带格局和资源补充研究#br#

为了解牡蛎在潮间带的生态特性及资源分布情况,调查了浙江三门健跳港上游湾区牡蛎潮间带分带格局,并检验附苗器类型和地点对牡蛎资源补充的影响。结果显示:潮间带定量调查共记录到3种牡蛎,即熊本牡蛎(Crassostrea sikamea)、近江牡蛎(C.ariakensis)和香港牡蛎(C.hongkongensis),首次在浙江海域记录到香港牡蛎的自然分布。熊本牡蛎在调查的5个潮区(1.4 m、2.2 m、3.0 m、3.8m、4.6 m)均有分布,其平均密度沿增加的高程梯度呈现上升趋势,其中4.6 m潮区熊本牡蛎密度显著高于1.4 m和2.2 m潮区(P<0.05)。近江牡蛎和香港牡蛎仅分布于最底层的1.4 m和2.2 m潮区。牡蛎资源补充实验结果表明,该湾区具有很高的熊本牡蛎资源补充量,采苗地点(P<0.05)和附苗器类型(P<0.05)均显著影响牡蛎苗的密度和壳高,总体上水泥片的附苗效果要好于塑胶轮胎,上游S1点附苗效果优于下游S2点,推荐上游S1点作为熊本牡蛎附苗场。