大鳍鳠产卵前后肌肉脂肪酸组成变化的研究

采用毛细管气相色谱法,对产卵前后大鳍鳠肌肉中的脂肪酸组成进行了测定。结果表明,大鳍鳠肌肉中脂肪酸种类丰富,含有22种脂肪酸,油酸(C18:1n-9)所占比例最高,其次为软脂酸(C16:0)、棕榈酸(C16:1n-7)、亚油酸(C18:2n-6),所占比例分别为22.38%~37.57%、18.86%~23.95%、7.00%~11.52%、7.46%~9.97%。EPA和DHA在大鳍鳠肌肉中占有较高的比例,EPA占总脂肪酸的5.39%,DHA占5.44%。在野生大鳍鳠产卵前后的22种脂肪酸中有8种饱和脂肪酸(SFA),6种单不饱和脂肪酸(MUFA)和8种多饱和脂肪酸(PUFA),大鳍鳠肌肉中脂肪酸在产卵前后表现出差异。     

闽东海域繁殖期雌性棘头梅童鱼亲鱼不同组织的脂肪酸组成分析

为获知棘头梅童鱼亲鱼性腺发育后期脂类及脂肪酸蓄积特点,采用内标定量法,对性腺发育至Ⅳ期的雌性棘头梅童鱼亲鱼肌肉、肝脏和卵巢组织中的脂肪及脂肪酸含量进行了测定,并比较分析了3种组织中的脂肪酸组成。结果表明:雌性棘头梅童鱼亲鱼卵巢、肝脏和肌肉中脂肪的质量分数分别为(4.84±0.05)%、(2.66±0.05)%和(0.56±0.05)%,不同组织间具有显著性差异(P<0.05)。雌性棘头梅童鱼亲鱼的卵巢、肌肉和肝脏中分别检出29种脂肪酸,含量最高的饱和脂肪酸依次为C16∶0、C18∶0和C14∶0;含量最高的单不饱和脂肪酸依次为C16∶1、C18∶1n9c和C20∶1;含量最高的多不饱和脂肪酸依次为C22∶6n3(DHA)、C20∶5n3(EPA)和C20∶4n6(ARA)。从相对含量来看,肌肉中饱和脂肪酸的质量分数为(58.22±0.24)%,显著高于肝脏(52.71±0.24)%和卵巢(36.42±0.24)%(P<0.05);卵巢中单不饱和脂肪酸的质量分数为(23.78±0.09)%,显著高于肌肉(16.15±0.07)%和肝脏(10.79±0.05)%(P<0.05);卵巢中多不饱和脂肪酸质量分数为(39.79±0.19)%,显著高于肝脏(36.49±0.21) %和肌肉(25.63±0.19)%(P<0.05)。肌肉、肝脏和卵巢中EPA和DHA的总量依次升高,质量分数分别为(19.34±0.15)%、(26.59±0.16)%和(27.78±0.13)%。卵巢、肝脏和肌肉组织中的DHA/EPA分别为1.95、2.28和1.89,n-3系和n-6系多不饱和脂肪酸的比值分别为3.29、3.81和6.25。     

红罗非和福寿鱼雌性亲鱼不同组织脂肪酸组成分析

Folch法提取红罗非鱼(Oreochromis sp.)和福寿鱼(O.mossambicus×O.niloticus)雌亲鱼不同组织(肌肉、肝胰脏和卵巢)中的脂质,GC法分析脂肪酸组成。结果显示,红罗非鱼和福寿鱼卵巢中总脂含量分别占湿重的16.16%和10.20%,显著高于肝胰脏中的总脂含量(P<0.05),而肝胰脏中的总脂含量又显著高于肌肉(P<0.05)。2种鱼肌肉、肝胰脏和卵巢中共检出18种脂肪酸,主要饱和脂肪酸(SFA)是C14:0、C16:0和C18:0,主要单不饱和脂肪酸(MUFA)是C16:1和C18:1n-9,主要多不饱和脂肪酸(PUFA)是C18:2n-6、C20:4n-6(ARA)、C20:5n-3(EPA)和C22:6n-3(DHA)。ARA在福寿鱼肌肉中含量最高(9.16%),显著高于2种试验鱼其他组织中的ARA含量。Σ(EPA+DHA)在红罗非鱼的卵巢和福寿鱼的肌肉、肝胰脏和卵巢中含量高于其它组织(5.09%～7.70%),福寿鱼肌肉中最高(7.70%),卵巢中次之(6.97%)。红罗非鱼肌肉、肝胰脏和卵巢中的ΣPUFA含量均低于福寿鱼相应组织。红罗非鱼与福寿鱼各组织之间n-3/n-6比率变化不显著,但均小于1,为0.12～0.41。红罗非鱼的肝胰脏和卵巢中,脂肪酸含量ΣSFA>ΣMUFA>ΣPUFA;红罗非鱼肌肉、福寿鱼肝胰脏和卵巢中,ΣMUFA>ΣSFA>ΣPUFA;福寿鱼肌肉中,ΣSFA>ΣPUFA>ΣMUFA。     

长丰鲢卵巢发育后期的肝脏、卵巢营养成分及脂肪酸变化

为了解繁殖期长丰鲢卵巢和肝脏营养状况,明确亲鱼的营养程度,对长丰鲢卵巢发育后期的肝脏和卵巢营养成分及脂肪酸组成进行了对比分析。结果显示:卵巢中的粗脂肪和粗蛋白含量先上升后下降,肝脏中的粗脂肪含量逐渐下降,粗蛋白基本稳定不变。在卵巢和肝脏中共检测到21种脂肪酸,其中含量较高的脂肪酸种类分别是C16∶0、C18∶1、C22∶6和C16∶0、C18∶0、C18∶1;在卵巢和肝脏中单不饱和脂肪酸含量丰富,占总脂肪酸含量分别为38.5%~43.1%和33.0%~40.7%;在高度不饱和脂肪酸中,二十二碳六烯酸(DHA)含量在卵巢和肝脏中都是最高,含量分别为12.9%~15.5%和3.4%~5.7%。研究揭示了长丰鲢卵巢发育后期的肝脏和卵巢在营养物质组成上的特点。     

饲料磷脂水平对巴丁鱼体组织脂肪酸组成的影响

本研究旨在探讨饲料磷脂水平对巴丁鱼体脂肪酸组成的影响。用5组饲料(磷脂添加水平分别为0%、1%、2%、3%、4%)对巴丁鱼进行56d的饲养试验。试验结果显示,各组肝脏、肌肉、腹脂的C18:3n3含量随饲料磷脂水平的升高而升高,C18:1n9含量随饲料磷脂水平升高而降低;4%磷脂组肌肉、肝脏组织的高不饱和脂肪酸的含量最高,而对照组的最低。各组肝脏、肌肉、腹脂的脂肪酸大类含量排序均为:饱和脂肪酸总量单不饱和脂肪酸总量多不饱和脂肪酸总量高不饱和脂肪酸总量,且都以C16:0、C18:1n9、C18:2n6为主要的饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸。研究表明,巴丁鱼肝脏、肌肉、腹脂的脂肪酸组成在一定程度上受到饲料脂肪酸组成的影响;饲料磷脂水平为4%时,肌肉、肝脏组织转化合成高不饱和脂肪酸的能力最强。     

秘鲁外海茎柔鱼资源丰度和补充量与海表温度的相关关系

茎柔鱼(Dosidicus gigas)广泛分布在东太平洋海域,是一种重要的经济捕捞种类。本文根据2003～2010年我国鱿钓船在秘鲁外海的生产统计数据和遥感获取的海表温度(SST)数据,利用典型相关性分析法和Marine Explore软件分析了茎柔鱼的资源丰度和补充量(以单位捕捞努力量渔获量为指标,t.d-1)与栖息海域20°N～20°S、70°W～110°W各渔区SST的相关性,获得影响秘鲁外海茎柔鱼资源丰度和补充量的SST因子,并建立关系模型。结果表明,当年资源丰度与SST的相关性最高值为5月份92.5°W、12.5°N海区(相关系数为0.94,P<0.001),该海区SST最适范围为29.8～30.5℃;下一年度资源补充量丰度指数与当年SST的相关性最高值为6月份98.5°W、11.5°N海区的(相关系数为0.93,P<0.01),该海区SST最适范围为29.1～30.5℃。同时,分别建立了基于SST因子的资源丰度预测模型(P<0.01)。研究认为,秘鲁外海茎柔鱼资源丰度和补充量可较好利用SST因子进行预测。     

黄海海域5种底栖型海水鱼类组织脂肪酸组成特征分析

本研究测定和评估了青岛地区5种底栖型海水鱼[(黄鮟鱇(Lophius litulon)、鲬(Platycephalus indicus)、长绵鳚(Zoarces viviparu)、角木叶鲽(Pleuronichthys cornutus)和带纹条鳎(Zebrias zebrinus)]的形体指标、肌肉和肝脏的粗成分,以及肌肉、肝脏、脑、眼、皮肤、肠道等组织的脂肪酸组成,以全面评估底栖型代表鱼类的脂肪和脂肪酸组成特征。实验鱼每种鱼9尾,3尾一组混样作为一个重复。结果显示,在5种鱼中,肌肉脂肪含量整体偏低,黄鮟鱇和带纹条鳎肌肉脂肪含量尤其低(0.3%~0.4%),但黄鮟鱇、鲬和角木叶鲽具有较高的肝脏脂肪含量(19%~29%),而带纹条鳎和长绵鳚肝脏脂肪含量偏低(4%~5%)。脂肪酸组成方面,黄鮟鱇肌肉中n-3系列长链多不饱和脂肪酸(long chain-polyunsaturated fatty acid, LC-PUFA)含量显著高于其他鱼,尤其以DHA最为明显。黄鮟鱇背肌中还具有最高含量的18:1n-9。长绵鳚背肌脂肪酸组成最明显的特征是较低的16:0含量和较高的20:4n-6及EPA含量。肝脏脂肪酸中,鲬和角木叶鲽具有显著高的18:1n-9等单不饱和脂肪酸含量,但其DHA含量较低。在黄鮟鱇、带纹条鳎和长绵鳚的大多组织中均有较高的DHA、20:4n-6和22:5n-3,但EPA含量较低。研究表明,即使同为底栖型鱼类,不同种类间脂肪和脂肪酸组成差异也非常显著。黄鮟鱇具有显著高的n-3 LC-PUFA含量,脂肪酸营养价值较高。     

渤海银鲳成熟卵巢生化成分分析

采用现代生化分析方法,研究了渤海银鲳卵巢组织的生化成分。结果表明:卵巢水分平均含量为68.83%,蛋白质平均含量15.45%,脂肪平均含量12.37%,灰分平均含量1.21%,糖类(碳水化合物)平均含量1.82%;卵巢组织的17种氨基酸中以谷氨酸含量最高,天冬氨酸次之,赖氨酸居于第三位;卵巢脂质中的主要脂肪酸排列顺序如下:C18∶1(n9&7)>C16∶0>C22∶6n3>C16∶1n7>C20∶5n3,饱和脂肪酸(SFA)占脂肪酸总量的18.95%~25.28%,单烯酸(MUFA)占24.67%~31.87%,多不饱和脂肪酸(PUFA)占19.72%~24.26%,EPA和DHA之和占16.27%~20.08%。     

5种经济头足类水产品加工工艺性质的研究

以新鲜的枪乌贼、太平洋褶柔鱼、短蛸、冷冻的柔鱼和茎柔鱼为原料,分别测定其质量组成及其一般化学成分,并比较了柔鱼和茎柔鱼的热加工特性。研究发现,5种头足类水产品的可食部分均超过70%,水分含量超过78%,粗蛋白含量为58%~83%,粗脂肪含量为4%~19%。在2种不同的热处理方式下,柔鱼和茎柔鱼外套膜沿体轴方向的收缩均大于环绕体轴方向的收缩,在蒸煮过程中,柔鱼的质量损失和水分流失均在5 min时达到最大,茎柔鱼在6 min时达到最大,在烘烤的过程中,2种柔鱼的质量损失随着水分流失的增加而增加。     

基于脂肪酸分析的太平洋黄鳍金枪鱼营养生态位研究

黄鳍金枪鱼(Thunnus albacares)是生态系统中的顶级捕食者, 是海洋生态系统能量流动及物质循环的重要一环。为研究太平洋黄鳍金枪鱼在个体发育过程中的摄食变化以及不同海域营养生态位差异, 根据 2019 年东太平洋和 2021 年中西太平洋海域金枪鱼渔船采集的黄鳍金枪鱼样本, 对其肌肉的脂肪酸组成及特征进行了分析研究。结果显示, 黄鳍金枪鱼肌肉检测出 34 种脂肪酸, 包括 16 种饱和脂肪酸(SFA), 8 种单不饱和脂肪酸(MUFA), 10 种多不饱和脂肪酸(PUFA)。黄鳍金枪鱼肌肉脂肪酸中 SFA 含量与 PUFA 含量呈负相关, MUFA 含量与 PUFA 含量呈正相关。SFA 含量与叉长呈显著负相关, MUFA、PUFA 含量与叉长呈显著正相关。中西太平洋和东太平洋黄鳍金枪鱼肌肉脂肪酸含量存在差异, 两海域之间脂肪酸组成平均不相似性为 18.85%, 主要差异来自 n-3 系列多不饱和脂肪酸(n-3 PUFA)、SFA、PUFA、C22∶6n3 (DHA)、Cl6∶0、Cl8∶1n9、MUFA、C22∶6n3/C20∶5n3 (DHA/EPA)、 C18∶0、n-6 系列多不饱和脂肪酸(n-6 PUFA)。主成分分析显示, 黄鳍金枪鱼的营养级越高, 肉食性越高, 以甲藻和硅藻为食源的生物对黄鳍金枪鱼的能量来源贡献越大。相反, 营养级越低的黄鳍金枪鱼主要摄食以细菌、陆地植物和植食性桡足类为食源的生物。主成分回归表明, 黄鳍金枪鱼随叉长增大, 其营养级增加, 肉食性提高, 食性逐渐由以甲壳类和头足类向虾和硬骨鱼等富含多种特征脂肪酸的生物转变。东太平洋黄鳍金枪鱼较中西太平洋肉食性更强, 营养级更高, 甲壳类对其能量贡献更大。     

Sex-based differences in fatty acid composition of adult walleye

Differences in reproductive strategies of male and female fishes are presumably accompanied by differences in nutrient allocation and predicted to lead to divergence in body composition between the sexes. We compared patterns of variation in fatty acid profiles of lipids extracted from ova, liver, muscle and visceral fat between mature male and female walleye (Sander vitreus) sampled from two wild spawning stocks. Fatty acid profiles differed significantly among body tissues in both males and females, with the strongest contrast between muscle and visceral fat. Significant differences in fatty acid composition between the sexes were found in liver, muscle and visceral fat tissues. Variation among sexes and populations was greater in liver than in the other tissues. Female livers had lower relative abundances of palmitic acid (PA, 16:0) and oleic acid (OA, 18:1(n-9)), and higher relative abundances of arachidonic acid (ARA, 20:4(n-6)), eicosapentaenoic acid (EPA, 20:5(n-3)) and docosahexaenoic acid (DHA, 22:6(n-3)) compared to male livers. In addition, female muscle had higher relative abundance of OA and lower relative abundance of DHA compared to male muscle. Our results illustrate the differential effects of reproductive demands on the biochemical composition of males and females and have implications for the analysis of fatty acid profiles in studies of wild fish populations.     

中国近海日本鳗鲡玻璃鳗体组织营养成分及氨基酸、脂肪酸组成的比较

为了解日本鳗鲡玻璃鳗的早期营养需求，分别对中国近海6个不同地点玻璃鳗体组织一般营养成分、氨基酸和脂肪酸含量进行了分析。结果显示，野生玻璃鳗体组织中水分含量为78.62%±2.03%，干重基础下粗蛋白质含量为69.08%±0.64%、粗脂肪含量为11.96%±1.33%、粗灰分含量为11.45%±1.30%。在台州、舟山、南通和东台4个采样点的样品中，水分含量无显著差异，而粗蛋白含量以舟山点最高、粗脂肪含量最低，并且舟山点样品肠道内发现可见内容物。玻璃鳗样品中氨基酸含量以谷氨酸、天门冬氨酸、赖氨酸、亮氨酸最高，半胱氨酸最低；除舟山点外，各采样点氨基酸含量无明显差异。不同洄游地点玻璃鳗组织中脂肪酸含量均以C16:0、 C18:1n9、 C20:5n3(EPA)、 C22:5n3、 C22:6n3(DHA)为主要成分，其中EPA和DHA共占脂肪酸总量的31.59%±1.00%，不同洄游地点样品必需脂肪酸含量差异明显。研究表明，必需氨基酸、必需脂肪酸对于玻璃鳗的正常生长存活具有重要意义。此外，对比不同地点营养物质组成及稚鱼饵料理论需求量分析，推测舟山点海洋环境中包含更适合玻璃鳗稚鱼摄食的食物，...     

Lipid content and fatty acid composition in female American shad,Alosa sapidissima,at different stages of ovarian development under reared conditions

The fatty acid accumulation in broodstock influences the fecundity of spawners as well as egg quality. A comprehensive understanding of the lipid and fatty acid changes in broodstock during the reproductive period can increase our knowledge of the nutritional requirements in artificial breeding programs. The study assessed the lipid and fatty acid compositions of muscle, liver and ovary of American shad at different stages of reproductive development under reared conditions. The results indicated that there were significant differences in lipid content among ovary, liver and muscle. Mean total lipid levels in ovary, liver and muscle were 15.92%–10.87%, 13.62%–6.89%, 10.39%–6.11% respectively. During the development season, the total lipid content in muscle in stages V and VI were significantly lower than those in stages II and III. The total lipid content in liver in stages IV, V and VI was significantly lower than in stages II and III, whereas the total lipid content in the ovary increased significantly as the gonad developed and reached its highest value at stage V. From II to VI stage, the liver content of docosahexaenoic acid (DHA) decreased and the ovarian content of DHA increased, whereas the content of eicosapentaenoic acid (EPA) remained relatively constant. The content of C16:0 and C18:1n‐9 decreased in muscle and liver, but significantly increased in ovary. It is therefore suggested that the lipids were preferentially transferred from muscle and liver to ovary beginning in stage IV as the gonad matured. At stage IV, the lipid transfer accelerated, and saturated and unsaturated fatty acids, such as C16:0, C18:1n‐9, DHA, EPA and arachidonic acid, were selectively transferred to and conserved in ovaries.     

Effects of starvation and feeding on the fatty acid profiles of Stage I phyllosoma of the spiny lobster, Jasus edwardsii

Fatty acid analyses were conducted on newly hatched and 8‐day‐old‐starved and fed Stage I phyllosoma larvae of the spiny lobster, Jasus edwardsii. Fed animals were offered excess 1.5 mm juvenile Artemia (enriched using the alga Isochrysis galbana, Tahitian isolate, T. iso.). After 8 days, there were significant increases in larval dry weight and the proportion of lipid in fed phyllosoma, whereas these parameters decreased in starved phyllosoma. The abundance of the saturated fatty acids 16 : 0 and 18 : 0 increased in both starved and fed phyllosoma, whereas the main monounsaturated fatty acids 16 : 1n‐7, 18 : 1n‐9 and 18 : 1n‐7 increased with feeding but decreased with starvation. There were no significant differences in the relative proportions of the highly unsaturated fatty acids (HUFAs) arachidonic (AA, 20 : 4n‐6), eicosapentaenoic (EPA, 20 : 5n‐3) and docosahexanoic (DHA, 22 : 6n‐3) acids between newly hatched and starved animals, whereas quantitatively DHA decreased with starvation and feeding. The DHA/EPA ratio was significantly lower in the starved and fed phyllosoma (0.5) compared with that found in the newly hatched phyllosoma (0.9). The lipid profiles of the newly hatched, starved and fed phyllosoma contained large amounts of n‐6 fatty acids resulting in low n‐3 : n‐6 ratios (2.8, 2.7 and 1.6 respectively). The importance of these results and the ability of enriched Artemia to provide a suitable fatty acid profile for this species are discussed.     

Effects of Frying-Chilling-Reheating on the Lipid Content and Fatty Acid Composition of Cultured Sturgeon (Huso huso,Beluga) Fillets

Slices of Huso huso were fried, chilled, and then reheated to evaluate for the changes in lipid characteristics. The total lipid content of raw sample was 3.09 g/100 g which consists of 29.1 g/100 g saturated fatty acids (SFAs), 42.554 g/100 g monounsaturated fatty acids (MUFAs), and 28.126 g/100 g polyunsaturated fatty acids (PUFAs). In fried samples, levels of C18 fatty acid groups, MUFAs, PUFAs, and n6/n3 ratio increased while SFAs, EPA, and DHA content decreased. Upon chill storage, C18:2 fatty acid and n6/n3 ratio decreased while n3 fatty acids increased slightly. Free fatty acid (FFA) decreased after frying, but peroxide value (PV) increased with subsequent decrease at chilled condition.     

Nutritional evaluation of fatty acids for the open thelycum shrimp, Litopenaeus vannamei: II. Effect of dietary n-3 and n-6 polyunsaturated and highly unsaturated fatty acids on juvenile shrimp growth, survival, and fatty acid composition

This study evaluated the nutritional value of dietary n‐3 and n‐6 polyunsaturated fatty acids (PUFA) such as linoleic (LOA) and linolenic (LNA) acids, and highly unsaturated fatty acids (HUFA) such as arachidonic (AA), eicosapentaenoic (EPA), and docosahexaenoic (DHA) acids for juvenile Litopenaeus vannamei, based on their effects on growth, survival, and fatty acid composition of hepatopancreas and muscle tissue. Diets contained 5% total lipid. A basal diet contained palmitic and stearic acids each at 2.5% of diet. Five diets contained 0.5% dry weight of LOA, LNA, AA, EPA, or DHA. An additional diet evaluated HUFA in combination by supplementing at 0.5% of diet, a mixture of n‐3 HUFA. All HUFA showed higher nutritional value than PUFA for shrimp and produced significantly (P < 0.05) higher final weight, weight gain, and total lipid in shrimp muscle. Fatty acid profiles of shrimp tissues reflected the composition of the dietary lipids. In general, saturated fatty acids were more abundant in the neutral factions, while PUFA and HUFA were more abundant in the polar fractions of tissues. Under these experimental conditions, HUFA had much greater nutritional value than PUFA for juvenile L. vannamei; moreover, dietary requirements for PUFA were not demonstrated.     

闽东海域银鲳亲鱼性腺发育后期脂类及脂肪酸蓄积特点

脂肪和脂肪酸是海水鱼类早期生长发育的重要结构物质和能量来源。亲鱼的脂肪和脂肪酸储备影响其繁殖性能和早期仔鱼的发育。为了获知银鲳亲鱼性腺发育后期脂类及脂肪酸蓄积特点,本研究采用氯仿甲醇法及气相色谱法定量检测了繁殖季节闽东海域野生银鲳亲鱼不同组织的总脂肪及脂肪酸含量。结果表明:亲鱼卵巢、精巢、肝脏和肌肉的总脂肪含量差异显著。卵巢、精巢、肝脏和肌肉的总脂含量分别为:35.76%,15.11%,22.07%和22.14%(占组织干重)。极性脂肪占总脂肪的比例在精巢中最高,其次为肝脏和卵巢,在肌肉中最低。性腺从Ⅳ期发育到Ⅴ期,雌鱼卵巢总脂肪和中性脂肪含量显著增加,雄鱼肌肉极性脂肪含量显著降低。中性脂肪中卵巢的20∶5n-3(EPA,2.25~3.87 mg/g)、22∶6n-3(DHA,6.71~13.03mg/g)和高不饱和脂肪酸(HUFAs,17.20~29.64 mg/g)含量最高,极性脂肪中精巢的EPA(0.38~0.27 mg/g)和DHA(3.12~3.59 mg/g)含量最高。性腺中n-3/n-6比值显著高于肝脏和肌肉中。随着卵巢发育,DHA等必需脂肪酸在雌鱼不同组织及同一组织不同脂肪类别之间存在转移现象。研究表明,银鲳亲鱼各组织的总脂肪含量、总脂肪组成及脂肪酸绝对含量(mg/g干物质)具有组织特异性,随着性腺发育,必需脂肪酸总体上表现为由肌肉和肝脏向性腺中转移,且性腺中脂肪酸的变化主要发生在中性脂肪中。     

Replacement of fish oil with a DHA‐rich Schizochytrium meal on growth performance,activities of digestive enzyme and fatty acid profile of Pacific white shrimp (Litopenaeus vannamei) larvae

This trial was conducted to evaluate the effects of replacing dietary fish oil with Schizochytrium meal for Pacific white shrimp (Litopenaeus vannamei) larvae (initial body weight 4.21 ± 0.10 mg). Six test microdiets were formulated using Schizochytrium meal to replace 0 g/kg, 250 g/kg, 500 g/kg, 750 g/kg, 1000 g/kg or 1500 g/kg fish oil DHA. No significant differences were observed in survival, growth, final body length and activities of digestive enzyme among shrimp fed different diets (p > .05). No significant differences were observed in C20:5n‐3 (EPA) in muscle samples (p > .05). C18:3n‐3 and C20:4n‐6 in muscle increased as Schizochytrium meal replacement level increased (p < .05). No significant differences were observed in C22:6n‐3 (DHA) and n‐3 fatty acids among shrimp fed diets that algae meal replaced 0 g/kg ‐ 1000 g/kg of fish oil. Shrimp fed diet R150 had higher DHA content than other groups and had higher n‐3 fatty acids than that of shrimp fed diets R50, R75 and R100 (p < .05). C18:2n‐6, PUFA and n‐6 fatty acids in muscle increased, while n‐3/n‐6 ratio decreased with increasing algae meal replacement level from 0 g/kg to 1000 g/kg (p < .05). In conclusion, Schizochytrium meal could replace 1500 g/kg fish oil DHA in the microdiets without negatively affecting shrimp larvae survival, growth and activities of digestive enzyme.