长江葛洲坝水利枢纽截流后长吻鮠的年龄与生长

对长江葛洲坝水利枢纽截流后长吻(?)年龄与生长进行了研究.采用脊椎骨鉴定年龄.得到体长与体重的关系式为W=2.5404×10~(-2)L~2.8164,椎体半径和体长相关式为L=8.2625+8.8414R;用von Bertalanffy方程对长吻(?)年龄和生长作了研究,得长吻(?)体重生长拐点在5.7465龄,应限制捕捞体重小于365l克,体长小于60厘米的长吻(?)个体.     

云龙湖水库青梢鲌的生长特性

采用鳞片和耳石对取自云龙湖水库的青梢鲌(Erythroculter dabryi)进行了年龄鉴定,并对其生长特性进行分析。结果显示:以鳞片和耳石为年龄鉴定材料时,二者的吻合率分别为59.57%和90.16%。年龄鉴定结果显示,所捕获的青梢鲌由1~8龄个体组成,主要是3~5龄个体;个体体长与耳石半径之间呈直线相关:L=9.5632R-0.1595(R2=0.995,n=175),体重与体长呈指数函数相关:W=1.07×10-2L3.3035,其生长规律符合von Bertalanffy方程:Lt=20.4741(1-e-0.2531(t+0.8056)),Wt=229.5864(1-e-0.2531(t+0.8056))3.3035;青梢鲌生长拐点年龄为3.92龄,对应的体长为14.28 cm,体重为69.88 g;3.92龄为合理的捕捞时期。     

新疆额尔齐斯河银鲫年龄鉴定比较与生长特征研究

以2013年4－10月从新疆额尔齐斯河采集的546尾银鲫（Carassius auratus gibelio）样本为材料,比较4种钙化组织（微耳石、鳞片、鳃盖骨和脊椎骨）年龄鉴定结果,选择最佳的年龄鉴定材料,研究其生长特征。结果表明,4种钙化组织中,微耳石磨片年龄读数的平均百分比误差（IAPE）最低（1.89%）,同一观察者2次读龄的吻合率最高（91.9%）,为4种材料中最为准确的年龄鉴定材料;采用微耳石进行年龄鉴定,银鲫样本由1～9龄组成,以3～4龄为主,占77.28%;银鲫体长与体重呈幂函数关系,雌雄群体间无显著性差异,体长与体重的关系为：W=2.661×10-5L3.085（R2=0.96,n=546）;生长规律用von Bertalanffy生长方程表示为：Lt=359.7 [1-e-0.172(t+1.066)], Wt=2042.3[1-e-0.172(t+1.066)]3.085;个体的生长拐点年龄为5.49龄,对应的体长及体重分别为243.2 mm和610.81 g。     

长江中上游大口鲶的年龄和生长

本文对长江上、中游干流的大口鲶进行了年龄与生长研究。脊椎骨是鉴定年龄的最佳材料,年轮形成时间4～6月,体长与体重关系式为W=1.28815×10~(-2)L~(2.920288),椎体半径和体长相关式为L=22.533+8.4413R,生长适合Von Bertalanffy生长方程式,为充分合理利用大口鲶资源,应限制捕捞4龄以前,体长79厘米以下的个体。     

武湖翘嘴红鲌年龄和生长的研究

报道了武湖翘嘴红鲌的年龄生长特点.其渔获物中2+和3+龄个体占57.1%,体长以300～600mm为主,体长与鳞长之间呈直线相关L=6.1814+90.4837 R,体重与体长呈指数函数相关W=2.045 ×10-6L3.2585,3+龄以前生长最快,生长指标高,体长和体重的相对增长率大,其生长规律符合Von Bertalanffy方程L1=1 404.9(1-e-0.10(t+0.71)),W1=3.629×14(1-e-0.10(t+0.71))3.2585,捕捞3龄以上个体较为合理.     

西藏雅鲁藏布江双须叶须鱼的年龄结构与生长特征

根据2008–2009年、2012年以及2013年采自雅鲁藏布江水系的956尾样本对双须叶须鱼(Ptychobarbus dipogon)的年龄结构和生长特征进行研究。雌性渔获物体长范围为146~569 mm,雄性渔获物体长范围为167~506 mm,性别未辨渔获物体长范围为78~297 mm;耳石上的年轮形成于每年的3–6月份;估算的雌性群体年龄范围为3~24龄,雄性群体年龄范围为3~13龄,性别未辨群体年龄范围为2~6龄。体长(Ls)–体重(W)方程分别为:W=2.494×10–5Ls2.877(雌性),W=2.790×10–5Ls2.856(雄性);拟合出来的von Bertalanffy生长方程为:Lt=606.9[1–e–0.114(t+0.163)],Wt=2538.4[1–e–0.114(t+0.163)]2.88(雌鱼);Lt=496.3[1–e–0.162(t–0.018)],Wt=1391.1[1–e–0.162(t–0.018)]2.86(雄鱼)。估算的雌鱼和雄鱼的拐点年龄分别为9.1龄和6.5龄,对应的体长和体重分别为396.0 mm、743.6 g和322.5 mm、406.3 g。研究表明,双须叶须鱼种群结构趋向低龄化,可能受全球气候变化、生物入侵和过度捕捞等因素的影响。针对这些影响因素,本研究提出了相应的渔业管理措施。     

东海中部日本鲭繁殖群体年龄与生长特征

根据 2021 年 3?4 月在东海中部采集的日本鲭(Scomber japonicus)繁殖群体样本, 通过耳石轮纹判读、生长逆算和生长方程拟合分析了其群体结构与生长特征。结果表明: 日本鲭繁殖群体中雌雄个体之间的叉长和体重关系不存在显著性差异(F=0.376, P=0.54＞0.05); 雌雄合计的年龄结构为 1~7 龄, 优势年龄组为 1~2 龄, 占比 73.63%; 3 龄及 3 龄以下, 雌性数量多于雄性; 3 龄以上, 雌雄数量基本相当, 但雌性缺少高龄个体。叉长与体重关系式为: W=0.8837×10^–6FL^3.47。线性函数关系是叉长和耳石半径关系的最优解, 其关系式为 FL=184.09R?112.73。利用 4 种模型逆算出的叉长和年龄数据来拟合 von Bertalanffy、Gompertz 和 Logistic 等 3 种生长方程并进行比较, 其中用 Dahl-Lea 模型逆算的 von Bertalanffy 方程的 AIC 值和 BIC 值均最小, 因此选择其作为最佳生长方程, 得到的 von Bertalanffy 叉长生长方程为 FLt=427.18[1?e^{–0.23(t+2.5)}]; 体重生长方程为 Wt=1187.20[1?e^{(–0.23(t+2.5)}] 3.47。经过二阶求导, 得到拐点年龄为 2.91 龄, 对应的拐点体重和叉长分别为 365.03 g, 304.09 mm, 可以作为今后制定日本鲭开捕规格的重要参考依据。     

雅鲁藏布江下游墨脱江段黄斑褶鮡的年龄结构与生长特征

于2018年3月、7月,在雅鲁藏布江墨脱江段采集黄斑褶鮡(Pseudecheneis sulcatus)203尾,其中183尾可用脊椎骨作为鉴定材料对其进行年龄鉴别,并对其进行生长特征分析。通过对比发现,脊椎骨比微耳石更适合做为黄斑褶鮡的年龄鉴定材料。所采样本的年龄范围为1~9龄,其中2~5龄为优势群体,占样本总数的73.22%。雌、雄个体生长无显著性差异,样本体长分布范围为50.0~144.0 mm,平均体长为(99.1±18.67)mm,体重分布范围为3.8~65.6 g,平均体重为(26.0±14.7)g。脊椎骨半径与体长的直线关系式:L=62.88R+40.69(R2=0.89,n=183)。体长与体重的关系式:W=3×10^-5L^2.9292(R2=0.96,n=183),通过对比Compertz GF、Logistic GF、von Bertalanffy生长方程(VBGF)三种生长方程的拟合效果,选用von Bertalanffy方程进行描述黄斑褶鮡生长特性,生长方程:Lt=189.56[1-e^-0.118[t+1.981]],Wt=155.45[1-e^-0.118[t+1.981]]2.9292;生长速度方程:dL/dt=22.37e^-0.118(t+1.981),dW/dt=53.57e^-0.118(t+1.981)(1-e^-0.118(t+1.981))1.9292;生长加速度方程:d^2L/dt^2=-2.639e^-0.118(t+1.981),d^2W/dt^2=6.34e^-0.118(t+1.981)(1-e^-0.118(t+1.981))0.9292(2.9292e^{-0.118(t+1.981}-1)。黄斑褶鮡体重生长拐点为7.1龄,对应的体长和体重分别为124.6 mm和45.5 g。黄斑褶鮡属小型,中速生长鱼类。雅鲁藏布江下游墨脱江段的黄斑褶鮡资源已遭到破坏,需加大力度进行保护。     

怒江细尾高原鳅生长特征与食性

对2008年从怒江采集到的172尾细尾高原鳅(Triplophysa stenura)进行了生长与食性的研究。并采用von Bertalanffy生长方程、Gompertz生长方程、Logistic生长方程以及三项式方程分别拟合了细尾高原鳅的生长。实验结果显示,耳石适合于细尾高原鳅的年龄鉴定。体长与体重关系式为W=0.9996×10-5L2.9762(R2=0.9680)。体长与耳石半径间关系式为L=0.0027R1.7230(R2=0.9542)。四种生长方程均能反映其生长规律,其中von Bertalan-ffy生长方程表达式为Lt=246.9430(1-e-0.05964(t-0.1689));Wt=132.0300(1-e-0.05964(t-0.1689))2.9762。体重、生长曲线的拐点为18.45龄。食性分析表明:细尾高原鳅为杂食性鱼类,食物组成主要是水生昆虫幼虫,着生藻类,原生动物及有机碎屑等。     

闽东北外海渔场银方头鱼渔业生物学初步研究

闽东北外海底层流刺网作业渔获的银头鱼群体由1～6龄11个世代构成,以2、3和4龄占优势;体长优势组为210～270mm,体重优势组为200～400g,体长与体重的关系W=2.1120×10~(-5)×L~(2.9964)r=0.9970。初次性成熟年龄为2～3龄,最小性成熟个体体长为190mm,在4～8月均有产卵活动;按von Bertalanffy方程拟合生长参数:L_(∝)=436.21mm,W_(∝)=1715.05g,k=0.2357,t_0=-0.9542,体重生长拐点年龄为3.702龄。     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Plasma estradiol-17 (E₂), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E₂ and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E₂ and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass.     

Cardiac,ventilatory and metabolic responses of two ecologically dissimilar species of fish to waterborne cyanide

Changes in heart rate, ventilatory activity and oxygen consumption were determined in trout (Salmo gairdneri) and brown bullhead catfish (Ictalurus nebulosus) during exposure to a steadily increasing concentration of waterborne cyanide selected to produce death in 8–9 hours for each species. The lethal cyanide concentration for the bullheads was an order of magnitude higher than for trout. Trout developed an immediate and gradually increasing bradycardia throughout the exposure period. Cyanide produced tachycardia in the bullhead followed by a gradual onset of bradycardia as the concentration of cyanide was raised. Pericardial injection of atropine (a muscarinic cholinergic antagonist) indicated that bradycardia in the trout was due initially to increased vagal tone but later due to the direct effect of cyanide on the heart. Hyperventilation in the trout persisted throughout the exposure period, although the rate and amplitude fluctuated and was variable between individual fish. During the last hour of exposure (highest cyanide concentration), ventilation was characterized by rapid, shallow breaths followed by a sudden respiratory arrest. The bullheads exhibited hyperventilation during the first 3 hours of exposure followed by a gradual, linear drop in ventilation rate and amplitude until death occurred. Cardiac and ventilatory responses in both species were attributed to stimulation of central and peripheral chemoreceptors by cyanide. Evidence is presented which suggests the initial response in the bullheads was due, at least in part, to gustatory stimulation by the cyanide. Oxygen consumption of the trout remained above pre-exposure levels for the majority of the test period. Oxygen consumption in the bullhead paralleled the changes in heart and ventilatory rates. Whole-body lactate levels of fingerlings of both species during cyanide exposure were measured to estimate the extent of anaerobiosis. Whole-body lactate levels were much greater in the bullheads than the trout, indicating a higher capacity for anaerobiosis, possibly due to a greater fuel supply. Overall, the trout responded to cyanide in a manner similar to that produced by environmental hypoxia whereas the bullheads experienced a gustatory stimulus which masked the hypoxia-like response.     

An overview of stress physiology of fish transport: changes in water quality as a function of transport duration

This study brings an integrated analysis about the relationship between water deterioration and its physiological consequences in live fish transport. The analysis was focused on the transport water and its deterioration, and physiological challenges imposed on the fish. Usual commercial handling procedures employed to mitigate fish stress during transport were discussed. Future topics of research for the establishment of safer fish transport protocols were proposed. Transport was classified into short (≤8 h) or long transport (>8 h). The main issue in short transports should be the prevention of water pH reduction, while in long transports it is the increase in ammonia. Plasma cortisol is the most employed marker for stress and is acutely elevated upon short episodes of transport, but remains elevated even in long‐transport events. Plasma glucose is perhaps a better marker for handling stress. Plasma lactate, pH, osmolality CO₂ and ions should be more often evaluated. Plasma Na⁺ and Cl^‐ are very useful markers of acidosis, due to their respective exchange for H⁺ and , for acid–base regulation. The establishment of species‐specific transport protocols should be preceded by such combined analyses of water and physiological parameters.     

Are hatchery‐reared abalone naïve of predators? Comparing the behaviours of wild and hatchery‐reared northern abalone,Haliotis kamtschatkana (Jonas, 1845)

Abalone populations have declined worldwide, generating interest in enhancement using hatchery‐reared individuals. In many cases, such restoration efforts have met with limited success due to high predator‐induced mortality rates. Furthermore, the mortality rates of outplanted hatchery abalone are often considerably higher than for wild individuals. This study uses northern abalone (Haliotis kamtschatkana) as a case study to determine whether hatchery‐reared abalone behave differently than their wild counterparts. In the field, outplanted hatchery‐reared abalone were significantly less responsive than wild abalone, in terms of number of abalone responding and intensity of response, to nearby movement and to physical contact with an inert probe. Also, when encountering a cue to which all abalone responded (a seastar predator), hatchery‐reared individuals remained subdued. Anti‐predator behavioural deficits in hatchery‐reared abalone were more pronounced in 4‐year‐old individuals than in 1‐year‐old individuals, suggesting an influence of either age or amount of time spent in the hatchery environment. These behavioural differences are expected to increase the vulnerability of hatchery‐reared abalone to predators, and are likely a major cause of their elevated predator‐induced mortality when outplanted.     

Effects of cadmium on the kinetics of calcium uptake in developing tilapia larvae, Oreochromis mossambicus

The toxic effects of Cd²⁺ on Ca²⁺ influx kinetics in developing tilapia (Oreochromis mossambicus) larvae were evaluated. Addition of 20 µg l^-1 of Cd²⁺ to the environment of 0 and 3 day-old larvae competitively inhibited the Ca²⁺ uptake within 4h resulting in a great increase in K_m values for Ca²⁺ influx (19.3 and 17.4 fold, respectively) as compared with their respective controls. Consequently, the actual Ca²⁺ influx of larvae in solutions of 0.2 mM Ca²⁺ are suppressed by 32–45%. Also, 3 day-old larvae were more sensitive to internally accumulated Cd²⁺ than 0 day-old larvae. Although the Ca²⁺ influx in 0 and 3 day-old larvae may be restored to the levels of their respective controls with 24h of being transferred to a 20 µg l^-1 Cd²⁺ solution, total body Ca²⁺ content was significantly reduced in 3 day-old larvae. Increased Ca²⁺ uptake efficiency ensures sufficient Ca²⁺ for normal growth. However, rapid increase in Ca²⁺ influx after hatching also leads to higher Cd²⁺ uptake. Exposure to Cd²⁺ will lead to a drop in body Ca²⁺ content resulting in retardation of larval growth. Therefore, we conclude that if Ca²⁺ uptake is interfered with at this critical stage of development, larvae will not be able to maintain normal levels of body Ca²⁺ and will show signs of Cd²⁺ poisoning.     

Migratory dynamics of stream-spawning longnose gar (Lepisosteus osseus)

Abstract– Literature evidence suggests that lake-dwelling longnose gar (Lepisosteus osseus) enter tributary streams to spawn, Until the present study, the dynamics of this breeding migration had never been investigated quantitatively. During the summers of 1991 and 1992, longnose gar were captured as they entered Weaubleau Creek, Missouri, a tributary of Harry S. Truman Reservoir. The in-stream spawning migration began in early April and ended in late May, and was positively correlated with stream flow and water level, and negatively correlated with water temperature. In-stream residence times ranged from 15 to 94 days, with males exhibiting longer residence times than females. Once in-stream, longnose gar travelled as far as 10 km upstream and occupied certain pools at greater relative frequencies. Although the reason for this preferential utilization is not completely understood, it may relate to pool depth and riffle proximity. Longnose gar disperse from the spawning stream great distances, with gar captured in Weaubleau Creek being recaptured up to 48 km away. This information should provide fisheries biologists the means to consider the reproductive ecology of this species in their conservation and management decisions.     

Nutritional regulation of hepatocyte fatty acid desaturation and polyunsaturated fatty acid composition in zebrafish (Danio rerio) and tilapia (Oreochromis niloticus)

The desaturation and elongation of [1-¹⁴C]18:3n-3 was investigated in hepatocytes of the tropical warm freshwater species, zebrafish (Danio rerio) and Nile tilapia (Oreochromis niloticus). The hepatocyte fatty acid desaturation/elongation pathway was assayed before and after the fish were fed two experimental diets, a control diet containing fish oil (FO) and a diet containing vegetable oil (VO; a blend of olive, linseed and high oleic acid sunflower oils) for 10 weeks. The VO diet was formulated to provide 1% each of 18:2n-6 and 18:3n-3, and so satisfy the possible EFA requirements of zebrafish and tilapia. At the end of the dietary trial, the lipid and fatty acid composition was determined in whole zebrafish, and liver, white muscle and brain of tilapia. Both zebrafish and tilapia expressed a hepatocyte fatty acid desaturation/elongation pattern consistent with them being freshwater and planktonivorous fish. The data also showed that hepatic fatty acid desaturation/elongation was nutritionally regulated with the activities being higher in fish fed the VO diet compared to fish fed the FO diet. In zebrafish, the main effect of the VO diet was increased fatty acid Δ6 desaturase activity resulting in the production of significantly more 18:4n-3 compared to fish fed the FO diet. In tilapia, all activities in the pathway were greater in fish fed the VO diet resulting in increased amounts of all fatty acids in the pathway, but primarily eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3). However, the fatty acid compositional data indicated that despite increased activity, desaturation of 18:3n-3 was insufficient to maintain tissue proportions of EPA and DHA in fish fed the VO diet at the same level as in fish fed the FO diet. Practically, these results indicate that manipulation of tilapia diets in commercial culture in response to the declining global fish oil market would have important consequences for fish fatty acid composition and the health of consumers. Scientifically, zebrafish and tilapia, both the subject of active genome mapping projects, could be useful models for studies of lipid and fatty acid metabolism at a molecular biological and genetic level. This revised version was published online in August 2006 with corrections to the Cover Date.     

Production of diatom–bacteria biofilm isolated from Seriola lalandi cultures for aquaculture application

Controlled incorporation of selected microalgae and bacteria in aquaculture systems can be beneficial because they can act as microbiological control. That is why the characteristics of biofilm generated naturally in Seriola lalandi culture cages were analysed, their potential benefit to the growth of larvae was studied, and their controlled use for improving the larval viability and as a vector to improve incorporation of previously studied probiotic bacteria was tested. According to biodiversity results, these biofilms are composed of a diatom–bacteria mix showing a decrease in biodiversity in laboratory culture conditions being dominated by Navicula phyllepta and bacteria of the family Rhodobacteraceae. This can be produced on mesh substrates incorporated in bioreactors with rapid growth rate and adhesiveness. Preliminary results from the addition of substrates with this specific biofilm in larvae culture systems showed that it is consumed by the larvae without negative effects, while positive effects on the viability of larvae in combination with probiotics were observed. Considering preliminary results, the addition of these specific substrates with diatom–bacteria biofilms could be a good improvement for aquaculture systems and together with the use of probiotics can contribute to maintaining a stable and controlled system improving the viability of the larval fish culture in its early stages.