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1.
利用替代寄主生产马尾松毛虫质型多角体病毒的研究   总被引:7,自引:0,他引:7  
DpCPV对8种鳞翅目昆虫感染试验结果表明,棉铃虫最敏感,感染率达97.0%。DpCPV对棉铃虫的毒力LC50=7.65×104CPB/ml,浓度与感染死亡率相关性及回归方程均达到显著性。HaDpCPV与原毒种DpCPV的形态特征相同;棉铃虫可用人工半合成饲料配方2在小塑料盒内单虫饲养,幼虫成活率达98%以上、羽化率达90%,平均蛹重0.4006g,平均产卵量797.7粒,卵孵化率达98.2%;HaDpCPV对第一代4~5龄马尾松毛虫的毒力较强,LC50=1.14×104CPB/ml,与原毒种相比,毒力没有下降。用棉铃虫来生产DpCPV,采用23~26℃、3龄虫、使用浓度105CPB/ml以上,是比较理想的组合。将病毒喷在人工饲料表面喂虫,可获得较高的病毒产量,平均每条虫可产8.13亿CPB,与用原宿主生产的产量相接近。用HaDpCPV防治第一、二代4~5龄马尾松毛虫,防治效果达60.2%~84.0%。  相似文献   

2.
Ha-DpCPV对4~5龄马尾松毛虫有一定毒力,浓度与感染死亡率的相关性达到显著水平,致死中浓度LC50为1.15×104CPB/ml.Ha-DpCPV与DpCPV比较,其毒力没有下降。  相似文献   

3.
应用室内保存一年的提纯杨毒蛾核型多角体病毒,感染2~4龄杨毒蛾幼虫。测定结果表明,此毒株毒力较强,2龄幼虫LC50为1.7l8×l02PIB/ml;3龄幼虫LC50为8.183×102PIB/ml;4龄幼虫LC50为8.60×102PIB/ml以2龄幼虫最敏感。感染剂量越大,死亡率越高;虫龄越小,防治效果越好。  相似文献   

4.
对马尾松毛虫质型多角体病毒(D.CPV)添加辅助剂的增效。试验表明:在D.CPv中分别加入1%活性炭、20%轻质碳酸钙、0.2%墨汁水三种辅助剂,在林间试验校正死亡率达80.6%以上,比单纯病毒防治松毛虫效果好。D.CPV浓度为2×10 ̄6CPB/ml加50%甘油贮裁(冰箱2一4℃)14个月,仍有较强的活性,致毒校正死亡率达73%,比未加甘油的病毒冰箱贮藏、常温贮藏分别增效8%、29.2%。  相似文献   

5.
用棉铃虫做为替代寄主生产马尾松毛虫质型多角体病毒应用马尾松毛虫质型多角体病毒(DPCPV)防治马尾松毛虫,效果达7O%以上,且具有待效作用,一次防治可控制5-6年不成灾。但是,利用马尾松毛虫生产DpCPV受季节及虫源的限制,同时还存在林间生产的病毒质...  相似文献   

6.
舞毒蛾核型多角体病毒毒力生物测定   总被引:2,自引:0,他引:2  
采用昆虫病毒毒力生物测定方法[1],以舞毒蛾 2 龄幼虫为对象,对大邑株舞毒蛾核型多角体病毒毒力进行测定,其 L C50 = 182×104 P I B/m l,95% 置信限上限为 241×104 P I B/m l,下限为 122×104 P I B/m l;用浓度 16×106 P I B/m l 和 16×105 P I B/m l 进行感染, 其 L T50分别为 738 天、824 天。  相似文献   

7.
应用蜡状芽孢杆菌防治棉蝗的初步研究   总被引:2,自引:0,他引:2  
蜡状芽孢杆菌(BacilluscereusFranklandandFrankland)是新发现的一种杀虫效率高,杀虫速度快的杀蝗微生物,用4.4×106和4.4×107孢子/ml接种棉蝗大龄跳蝻及成虫,棉蝗的死亡率分别达到82.6%和80.8%。毒力测定结果LC50为2.7×108孢子/ml。林间大面积试验,杀虫效果达77.9%。  相似文献   

8.
刘波  杨军 《吉林林业科技》1998,(5):17-18,39
本文报道了分月扇舟娥核型多角体病毒的室内毒力测定。结果表明,接种3龄幼虫可湿性粉剂的致死中浓度LC50为1.43×10^2PIB/ml、乳剂LC50为3.25×10^2PIB/ml,与病毒标准品LC50的52.23PIB/ml相比差异不显著,可湿性粉剂的LC50经乳剂的LC50低56%,并有缩短潜伏期的趋势,表明可湿性粉剂杀虫效果优于乳剂。致死中时LT50值随感染浓度增加而减少,同一浓度感染不同虫  相似文献   

9.
将不同的苏云金杆菌菌株Bt1.Bt2、Bt3及白僵菌菌株5SO3、LS、GD,配制成不同浓度的悬浮液,饲喂感染3龄云南松毛虫幼虫。实验结果表明:生物活性较强的菌株有Bt2及5SO3,其感染浓度与死亡率值回归方程式为Y(Bt2)=-3.8162+1.1665x;Y(5SO3)=-2.3274+1.0062x。致死中浓度LO_(50)为3.614×10 ̄7细菌/ml和1.917×10 ̄7孢子/ml;致死中时间LT_(50)分别为2.641天和7.619天。  相似文献   

10.
为害水杉和银杏的标毛虫paralebedaplagiferawalker幼虫经人工感染核型多角体病毒(PpNPV)后,第4天便开始出现死亡,25天内最高死亡率可达90%。病毒的致死中浓度(LC50)为1.49×104±3×102PIB/ml(P=0.5).害虫死亡率与感染浓度呈正相关,试验表明PpNPV对栎毛虫有较强的毒力。  相似文献   

11.

In Scandinavia, moose (Alces alces L.) sometimes cause severe browsing damage to economically-important pine. Moose-vehicle accidents have spurred construction of fences along roads, and these may interfere with moose migration between summer and winter ranges, or the road alone may be a barrier. If this happens and moose build up along roads, landowners may suffer economically. Therefore, this study investigated whether roads, fences or other factors influence the use of young pine stands by moose. Eighty stands along roads in northern Sweden were evaluated in which individually-browsed branches were counted on 9972 pines. Moose browsing was not significantly related to birch (Betula pendula Roth, B. pubescens Ehrh.) density, nor did it differ between pines (Pinus contorta Douglas or P. sylvestris L.). However, increased pine density, site productivity and proximity to a highway were associated with increased browsing. Further large-scale studies are needed to understand moose habitat selection and the effects of roads.  相似文献   

12.

The root systems of 2-yr-old Picea glauca, Picea mariana and Pinus banksiana seedlings were submitted to various frost temperatures during an artificial frost to induce different levels of root damage. Frost-damaged and control seedlings were placed in a greenhouse under high and low soil moisture regimes. Seedling growth and physiology were evaluated periodically. Seedling survival was reduced when root damage reached levels of 60-80%. Root systems of all three species showed partial to total recovery by the end of the experiment. In general, root freezing damage caused reductions in seedling growth, with these reductions becoming less significant over time. Root damage had little to no effect on black spruce and jack pine seedling physiology, while white spruce CO 2 uptake decreased with increasing root damage. Shoot nitrogen content of all three species decreased slightly with increasing root damage.  相似文献   

13.
Contour hedgerows of multipurpose tree species in the sloping tea lands of Sri Lanka are expected to reduce soil erosion and also add significant amounts of plant nutrients to the soil via periodic prunings. The objective of this experiment was to characterize the biomass decomposition pattern and quantify the amount of nutrients added through prunings of six tree species (Calliandra calothyrsus, Senna spectabilis, Euphatorium innulifolium, Flemingia congesta, Gliricidia sepium and Tithonia diversifolia) currently being used in hedgerows associated with tea. Withered leaf and stem prunings (50 g) were enclosed in 2-mm litter bags, placed at 5-cm depth and retrieved after one, three, six, nine and 12 weeks. Loss of initial dry weight, N, P and K was measured. Single exponential decay function adequately described both dry weight and nutrient loss. Tree species differed significantly in their rate of breakdown with decomposition constants (k) varying from 0.0299 to 0.2006 week−1 for leaves and from 0.0225 to 0.0633 week−1 for stems. Gliricidia showed the highest k for leaves with the rest in the following descending order: Senna > TithoniaEuphatorium > Calliandra > Flemingia. A similar pattern was observed for loss of all nutrients with Calliandra and Flemingia always having lower k values than the rest. Although N immobilization was not observed, immobilization of P and K was observed during the first week of incubation in some species, particularly in stem prunings. Annual biomass of prunings differed significantly between tree species in the following descending order: Calliandra > Senna > Flemingia > Tithonia > Gliricidia > Euphatorium. Calliandra added the greatest amount of nutrients annually to the soil with Euphatorium adding the least. Calliandra prunings provided the annual total K requirement and 49% of the N requirement of mature tea. However, none of the species provided more than 5% of the P requirement. It is concluded that among the tree species tested, Calliandra and Flemingia are the most suitable for contour hedgerows in tea plantations of this agroclimatic region because of their higher soil nutrient enrichment capacity and slower decomposition rates which would minimize leaching losses. This revised version was published online in June 2006 with corrections to the Cover Date.  相似文献   

14.

The effects of seed weight and seed type on seedling growth of Pinus sylvestris (L.) were studied by seeding individually weighed orchard and stand seed in different mixtures under harsh (direct seeding in field) and optimal (seeding in nursery) conditions. In the nursery experiment an increase in the seed weight from 3 to 7 mg increased the seedling height by 10-27% and total weight by 27-113%, and decreased the height/diameter ratio by 5-6% after 2 yrs. With elimination of seed weight effects, orchard seedlings were 2% taller than stand seedlings in year 2. Without elimination of seed weight effects, orchard seedlings were 7-13% taller. In the field experiment an increase in the seed weight from 3 to 7 mg increased seedling height by 18-65%, stem volume by 81-274% and the number of top-buds by 23-34% in year 5. After elimination of seed weight effects, orchard seedlings were 7-13% taller than stand seedlings and without elimination of seed weight effects 20-21% taller after 5 yrs. Even after elimination of both seed weight and genetic effects orchard seedlings were 3-9% larger than stand seedlings in the field experiment. In conclusion, the influence of seed weight and seed type on growth traits and slenderness is highly significant and the influence seems to be greater in harsh conditions.  相似文献   

15.
Baobab leaves form an important part of the local diet in Sahel countries and elsewhere in Africa. Existing leaf nutritional data and agroforestry performance information are based solely on Adansonia digitata L., the baobab of continental Africa. The introduction potential of Adansonia species from the center of diversity in Madagascar and from Australia remains untapped. To assess this potential, the mineral contents and B1 and B2 vitamin levels of dried baobab leaves were determined for five-year old trees of A. digitata, A. gibbosa (A. Cunn.) Guymer ex D. Baum, A. rubrostipa Jum. & H. Perrier (syn. A. fony Baill.), A. perrieri Capuron and A. za Baill. grown in an introduction trial in Mali. Nutritional data were evaluated against survival and vigor to identify promising germplasm. Leaf vitamin and crude protein contents were highest in the Madagascar species, especially A. rubrostipa (B1 88 mg 100 g−1, B2 187 mg 100 g−1, protein 20.7% dry weight). However, the local species far outperformed the introductions in survival, tree height, basal diameter and resistance to termites. We suggest grafting as a way of harnessing the vigor of well-adapted local baobab varieties to the superior nutritional profiles of A. rubrostipa and others. Cross-species grafting tests in Adansonia were successful, thus creating new agroforestry possibilities with different scion/rootstock combinations.  相似文献   

16.
Abstract

Wood in general and wooden studs in particular are often distorted owing to uneven shrinkage during the drying process in the sawmill. Twist is often the most detrimental of all types of distortion, and it is caused by spiral grain in combination with variations in moisture content. For sawmills, the objective is to produce dried, straight boards, and one method of dealing with boards with excessive spiral grain is to sort them out and then dry them in a pretwisted position to obtain straight boards after drying. A model using the finite element (FE) method for the simulation of drying twist distortions was first calibrated against laboratory experiments in which boards were dried with and without restraints and pretwists. After the calibration, the FE results were compared with industrial test results for boards that were dried without restraints or with restraints with zero pretwist, i.e. straight restraints. The FE model used an elastic–ideally plastic material model to obtain permanent deformations. The calibration was to set the yield stresses so that there was a good match between FE results and results from the laboratory experiments. The comparison between the industrial test results and the FE results showed that the FE model is capable of realistic simulations of drying boards with and without restraints and presumably also pretwists.  相似文献   

17.

This study investigated the stand structure in pine, spruce and deciduous forests in the border district of Finland and Russia. A total of 46 mature forest stands was selected as pairs, the members of each pair being as similar as possible with respect to their forest site type, age, moisture and topography. The stands were then compared between the two countries by means of basal areas and number of stems. The proportions of dominating tree species were 2-12% lower, and correspondingly the proportions of secondary tree species higher, in Russian forests. The density of the forest stock was also higher in each forest type in Russia. The forests in the two countries differed most radically in terms of the abundance of dead trees. The amount was two to four times higher in Russian deciduous and spruce forests, and in pine forests the difference was 10-fold. The stand structures indicated that Russian coniferous stands, in particular, were more heterogeneous than intensively managed pine and spruce stands in Finland.  相似文献   

18.

The root collar diameter and the height:diameter ratio are of particular importance in container-grown seedlings where a high density in the containers may produce spindly seedlings. Temperature regimes and light quality are known to affect plant growth. The aim of this study was to identify responses in Picea abies (L.) Karst. seedlings grown with light providing different red:far-red ratios and under temperature regimes with alternating day (DT) and night temperature (NT) from negative (DT < NT) to positive (DT > NT) difference (DIF) between DT and NT. Experiments were conducted in controlled environment chambers and in a daylight phytotron. Only limited thermoperiodic responses appeared in P . abies seedlings with respect to seedling height and dry weight accumulation. The formation of terminal buds, however, was clearly delayed in seedlings grown at negative DIF. The results indicate a requirement for day extension light that is high in far-red, to prevent terminal bud formation under natural short-day conditions. An extended study should be conducted to clarify the minimum level of light intensity and the optimal light quality needed to prevent terminal bud formation under natural short-day conditions.  相似文献   

19.
本文分析了CAD在设计中引起的正面和负面影响,并进行了系统的阐述,从而使设计者在应用中能够保持客观的态度。  相似文献   

20.
Heterobasidion parviporum and Heterobasidion annosum are widely distributed root‐rot fungi that infect conifers throughout Europe. Infection of conifer stumps by spores of these pathogens can be controlled by treating fresh stumps with a competing non‐pathogenic fungus, Phlebiopsis gigantea. In this study, growth of three Latvian strains of P. gigantea and the biological control agent ‘Rotstop’ strain was evaluated in stem pieces of Norway spruce, Scots pine, lodgepole pine, Douglas‐fir, Weymouth pine, Siberian larch and Sitka spruce. The growth rates of one H. parviporum and one H. annosum isolate were also measured in the same stem pieces. The growth rate of P. gigantea varied greatly in wood of different conifer species. It was higher in the three pine species, lower in Norway spruce and lowest in Sitka spruce and Siberian larch, and in Douglas‐fir, this fungus did not grow. The largest area of wood occupied by P. gigantea was in lodgepole pine. Growth of Latvian isolates of P. gigantea in the wood of Pinus and Picea species was comparable to that of the Rotstop isolate. Consequently, stump treatment with local P. gigantea isolates should be recommended. However, our results suggest that Douglas‐fir stump treatment against Heterobasidion by P. gigantea may be ineffective and other stump treatment methods should be considered.  相似文献   

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