Peripheral Concentration of Hydroxyprolineand 3-methyl-histidine in Postpartum Cow

1　Introduction　　 It has been show in rat(Harkness and Harkness,1 95 4) ,human(Montford and PereaTamayo,1 96 1 ) and cattle (Kaidi,1 989) that the increase in the weight of the uterus duringpregnancy is associated with an increase in the collagen content of the uterus.Themechanisms involved in timulating collagen synthesis are unknown.Atthe same time there ishypertrophy and hyperplasia of the smooth muscle fibers of the myometrium.In the post-partum period,the involutionary changes of the…     

Influence of different nitrogen application on flour properties,gluten properties by HPLC and end-use quality of Korean wheat

This study was performed to identify how the different levels of nitrogen application affected the variances of gluten properties and end-use qualities and the differences of variances among Korean wheat cultivars. Protein and dry gluten content, SDS sedimentation volume and water absorption of Mixolab increased as nitrogen application increased. This ratio of the increase was higher in Korean wheat cultivars for bread than in Korean wheat cultivars for noodles and cookies. The proportion of(α+β)-gliadin measured by reversed-phase high-performance liquid chromatography(RP-HPLC) increased, but the proportion of ω-and γ-gliadin decreased as the protein content increased. The Korean wheat cultivars for bread showed a high proportion of(α+β)-gliadin increase, the Korean cultivars for noodles had a high proportion of γ-gliadin decrease and the Korean wheat cultivars for cookies had a high proportion of ω-gliadin decrease. However, there was no variation of the component in the proportion of glutenin component measured by RP-HPLC, even though the protein content was increased, but all of the protein fractions measured by size exclusion(SE)-HPLC were increased. The soluble monomeric protein showed a high proportion of Korean wheat cultivars for bread by the increase of protein content. Bread loaf volume increased by the increase of protein content but there were no variances in the ratio of increase among Korean wheat cultivars. The cookie diameter decreased with the increase of protein content, and this ratio of decrease was the highest in Korean wheat cultivars for cookies. The hardness of cooked noodles also increased by the increase of protein content but there were no variations in springiness and cohesiveness. The decrease proportion of ω-gliadin affected the increase of bread loaf volume, the hardness of cooked noodles, and the decrease of cookie diameter.     

Improving winter wheat grain yield and water-/nitrogen-use efficiency by optimizing the micro-sprinkling irrigation amount and nitrogen application rate

Available irrigation resources are becoming increasingly scarce in the North China Plain (NCP),and nitrogen-use efficiency of crop production is also relatively low.Thus,it is imperative to improve the water-use efficiency (WUE) and nitrogen fertilizer productivity on the NCP.Here,we conducted a two-year field experiment to explore the effects of different irrigation amounts (S60,60 mm;S90,90 mm;S120,120 mm;S150,150 mm) and nitrogen application rates (150,195 and 240 kg ha~(–1);denoted as N1,N2 and N3,respectively) under micro-sprinkling with water and nitrogen combined on the grain yield(GY),yield components,leaf area index (LAI),flag leaf chlorophyll content,dry matter accumulation (DM),WUE,and nitrogen partial factor productivity (NPFP).The results indicated that the GY and NPFP increased significantly with increasing irrigation amount,but there was no significant difference between S120 and S150;WUE significantly increased first but then decreased with increasing irrigation and S120 achieved the highest WUE.The increase in nitrogen was beneficial to improving the GY and WUE in S60 and S90,while the excessive nitrogen application (N3) significantly reduced the GY and WUE in S120 and S150 compared with those in the N2 treatment.The NPFP significantly decreased with increasing nitrogen rate under the same irrigation treatments.The synchronous increase in spike number (SN) and 1 000-grain weight (TWG)was the main reason for the large increase in GY by micro-sprinkling with increasing irrigation,and the differences in SN and TGW between S120 and S150 were small.Under S60 and S90,the TGW increased with increasing nitrogen application,which enhanced the GY,while N2 achieved the highest TWG in S120 and S150.At the filling stage,the LAI increased with increasing irrigation,and greater amounts of irrigation significantly increased the chlorophyll content in the flag leaf,which was instrumental in increasing DM after anthesis and increasing the TGW.Micro-sprinkling with increased amounts of irrigation or excessive nitrogen application decreased the WUE mainly due to the increase in total water consumption (ET)and the small increase or decrease in GY.Moreover,the increase in irrigation increased the total nitrogen accumulation or contents (TNC) of plants at maturity and reduced the residual nitrate-nitrogen in the soil (SNC),which was conducive to the increase in NPFP,but there was no significant difference in TNC between S120 and S150.Under the same irrigation treatments,an increase in nitrogen application significantly increased the residual SNC and decreased the NPFP.Overall,micro-sprinkling with 120 mm of irrigation and a total nitrogen application of 195 kg ha~(–1) can lead to increases in GY,WUE and NPFP on the NCP.     

Effect of Bensulfuron-Methyl on Growth of Chlorella pyrenoidosa

To study the growth effects of differing concentrations of bensulfuron-methyl on Chlorella pyrenoidosa and to evaluate the ecological risk, the effects of bensulfuron-methyl on the growth and the content change of chlorophyll and protein in Chlorella pyrenoidosa were studied through 96 h acute toxicity tests. Bensulfuron-methyl accelerated the growth of algae at lower concentrations （〈 1 mg L^-1） with content increase of chlorophyll or protein, and it inhibited the growth of algae at higher concentrations （〉 5 mg L^-1）. The content of chlorophyll or protein in algae cells reduced with the increasing concentration of bensulfuron-methyl, exhibiting the good concentration-effect relationship. The 96 h-EC50 of bensulfuronmethyl upon the algae was 15.7 mg L^-1 Bensulfuron-methyl has inhibiting effect on the growth of Chlorella pyrenoidosa and is low in toxicity.     

Efficiency of potassium-solubilizing Paenibacillus mucilaginosus for the growth of apple seedling

Chemical potassium(K) fertilizer is commonly used in apple(Malus domestica L. Borkh) production but K is easily fixed by soil, resulting in reduced K fertilizer utilization and wasted resources. K-solubilizing bacteria(KSB) can cost-effectively increase the soluble K content in rhizosphere soil. Therefore, the objectives were to select high-efficiency KSB from apple orchards under various soil management models and evaluate their effects on apple seedling growth. Maize(Zea mays L.) straw mulching(MSM) increased the total carbon(TC), total nitrogen(TN) and available potassium(AK) in the rhizosphere and improved fruit quality. The number of KSB in the rhizosphere soil of MSM was 9.5×104 CFU g–1 soil, which was considerably higher than that in the other mulching models. Fourteen KSB strains were isolated with relative K solubilizing ability ranging from 17 to 30%, and five strains increased the dry weight per apple seedling. The most efficient strain was identified as Paenibacillus mucilaginosus through morphological observation and sequence analysis of 16 S rDNA, named JGK. After inoculation, the colonization of JGK in soil decreased from 4.0 to 1.5×10~9 CFU g~(–1) soil within 28 d. The growth of the apple seedlings and the K accumulation in apple plants were promoted by irrigation with 50 mL JGK bacterial solution(1×10~9 CFU mL~(–1)), but there was no significant increase in the AK content of rhizosphere soil. High-performance liquid phase analysis(HPLC) data showed that the JGK metabolites contained phytohormones and organic acids. Hence, the JGK strain promoted the growth of two-month-old apple seedlings by stimulating function of the produced phytohormones and enhanced K solubility by acidification for apple seedling uptake. This study enriches the understanding of KSB and provides an effective means to increase the K utilization efficiency of apple production.     

Effects of Waterlogging on Photosynthesis and Antioxidant Enzyme Activities of Six Barley Genotypes with Different Waterlogging Tolerance

A field experiment was carded out to study genotypic difference in the effect of waterlogging on photosynthesis, chlorophyll content and antioxidative enzyme activities in barley. Waterlogging caused a rapid decline in net photosynthetic rate (Pn) and stomatal conductance (gs), and little change in chlorophyll content during early days of the treatment. A dramatic increase in malondialdehyde (MDA) content, superoxide dismutase (SOD) and peroxidase (POD) in waterlogged plants in the early days of the experiment was found, indicating the occurrence of oxidative stress in barley plants exposed to waterlogging. There was a highly significant difference in the changed extent of all these parameters among genotypes.Franklin and Yongjiahong Liuleng Damai, which were relatively sensitive to waterlogging in terms of growth, photosynthesis and chlorophyll content, accumulated much more MDA than the other two relatively tolerant genotypes (93-3143 and QS).After removal of waterlogging, the genotypic difference became much greater in recovering of these examined parameters.Yongjiahong Liuleng Damai showed higher recovery, while Franklin only recovered to 50% of the control at the 14 day after waterlogging removal. It may be concluded that it is the difference in anti-oxidative stress caused by waterlogging that account for the major difference in photosynthesis among barley genotypes.     

接种摩西球囊霉对盐胁迫条件下盐地碱蓬叶片SOD和CAT活性的影响(英文)

[Objective] Under salt stress condition,effects of inoculation of arbuscular mycorrhizal fungi Glomus mosseae on SOD and CAT activity in Suaeda salsa seedlings under salt stress were studied.[Method]There were 2 NaCl levels,namely 0 and 400 mmol/L and each NaCl contained 2 treatments,one is inoculated by Glomus mosseae and the other is control.The growths of Suaeda salsa,SOD and CAT activities as well as MDA content in leaves was determined.[Result]Under salt stress condition,Glomus mosseae could increase the growths of Suaeda salsa,SOD and CAT activities in leaves and decreased MDA content in leaves.[Conclusion]It preliminarily demonstrated that Arbuscular Mycorrhizal(AM)Fungi could increase salt resistance of Suaeda salsa by increasing the activities of SOD and CAT as well as alleviating membrane injury.     

A Novel Three-Dimensional Mouse Embryonic Implantation Model In Vitro

To regenerate three-dimensional endometrium in vitro as a novel model for studying the mechanism of implantation of embryos, the luminal epithelial cells and stromal cells of the rabbit uterus were separated and cultured in vitro. The type Ⅰ mouse tail collagen was used as scaffolding material. The stromal cells were inoculated in the type I mouse tail collagen, and the luminal epithelial cells were inoculated on the type i mouse tall collagen to regenerate the endometrium in vitro. The regenerated endometrium was cultured in DMEM-F/12 media containing 100 nmol L^-1 progesterone, 10 nM β-estradiol, and 10% fetal bovine serum （FBS） for 3 d. The media were then replaced with CZB containing 100 nM progesterone, 10 nmol L-1 β-estradiol, and 10% FBS, and the mouse blastulas were co-cultured with it. The results of scanning electronic micrography showed that the epithelial cells on the surface of the reconstructed endometrium were covered with numerous slender microvilli and some epithelial cells protruded pinopodes. After culturing for 12 h with the mouse blastula, the shedding, attachment, and implantation of the blastula were observed. The blastula can escape from zona pellucida and attach to the three-dimensional endometrium and is then implanted into it. This study showed that the reconstructed three-dimensional endometrium can serve as a robust embryo implantation model in vitro.     

Evaluation of soil flame disinfestation(SFD) for controlling weeds,nematodes and fungi

Soil flame disinfestation(SFD) is a form of physical disinfestation that can be used both in greenhouses and on field crops. Its use for soil disinfestation in different crop growing conditions makes it increasingly attractive for controlling soilborne pathogens and weeds. But little is known about the effect on weeds and soilbrone diseases. This study reports on greenhouses and field crops in China that determined the efficacy of SFD to control weeds, nematodes and fungi. It also determined the impact of SFD on the soil physical and chemical properties(water content, bulk density, NO_3~–-N content, NH_4~+-N content, conductivity and organic matter) in three field trials. A second generation SFD machine was used in these trials. SFD treatment significantly reduced weeds(87.8%) and root-knot nematodes(Meloidogyne incognita)(98.1%). Plant height and crop yield was significantly increased with SFD treatment. NO_3~–-N and NH_4~+-N increased after the SFD treatment, and there was also an increase in soil conductivity. Water content, bulk density and organic matter decreased significantly in the soil after the SFD treatment compared to the control. Soil flame disinfestation is a potential technique for controlling weeds and diseases in greenhouses or in fields. SFD is a non-chemical, safe, environmentally-friendly soil disinfection method.     

Constitutive expression of feedback-insensitive cystathionine γ-synthase increases methionine levels in soybean leaves and seeds

Soybean(Glycine max(L.) Merr.) is a major crop that provides plant-origin protein and oil for humans and livestock.Although the soybean vegetative tissues and seeds provide a major source of high-quality protein,they suffer from low concentration of an essential sulfur-containing amino acid,methionine,which significantly limits their nutritional quality.The level of methionine is mainly controlled by the first unique enzyme of methionine synthesis,cystathione γ-synthase(CGS).Aiming to elevate methionine level in vegetative tissues and seeds,we constitutively over-expressed a feedback-insensitive Arabidopsis CGS(At D-CGS) in soybean cultivars,Zigongdongdou(ZD) and Jilinxiaoli 1(JX).The levels of soluble methionine increased remarkably in leaves of transgenic soybeans compared to wild-type plants(6.6-and 7.3-fold in two transgenic ZD lines,and 3.7-fold in one transgenic JX line).Furthermore,the total methionine contents were significantly increased in seeds of the transgenic ZD lines(1.5-to 4.8-fold increase) and the transgenic JX lines(1.3-to 2.3-fold increase) than in the wild type.The protein contents of the transgenic soybean seeds were significantly elevated compared to the wild type,suggesting that the scarcity of methionine in soybeans may limit protein accumulation in soybean seeds.The increased protein content did not alter the profile of major storage proteins in the seeds.Generally,this study provides a promising strategy to increase the levels of methionine and protein in soybean through the breeding programs.     

Study on Lysine and Methionine Content Promotion of Soybean Meal by Probiotic Fermentation Process

Soybean meal (SBM) is commonly used for livestock feeds, but its application in diets for livestock is limited due to some antinutritional factors. The contents of methionine and lysine of soybean meal were promoted by Bacillus natto and Leuconostoc mesenteroides fermentation, benefial for the livestock feeds. It was crude protein (CP) 56.8%, methionine 43.56 mg · g-1, and lysine 74.87 mg · g-1, cows fed a diet with FSBM milk yield raised 14.2%, the change in the milk protein, the lactose and the dry matter content had also obvious increase. This convenient technique offers helpful exploration for industrialization of soybean meal fermentation.     

The positive function of selenium supplementation on reducing nitrate accumulation in hydroponic lettuce (Lactuca sativa L.)

High nitrate(NO_3~- ) in vegetables, especially in leaf vegetables poses threaten to human health. Selenium(Se) is an important element for maintaining human health, and exogenous Se application during vegetable and crop production is an effective way to prevent Se deficiency in human bodies. Exogenous Se shows positive function on plant growth and nutrition uptake under abiotic and/or biotic stresses. However, the influence of exogenous Se on NO_3~- accumulation in hydroponic vegetables is still not clear. In the present study, hydroponic lettuce plants were subjected to six different concentrations(0, 0.1, 0.5, 5, 10 and 50 μmol L–1) of Se as Na2 Se O3. The effects of Se on NO_3~- content, plant growth, and photosynthetic capacity of lettuce(Lactuca sativa L.) were investigated. The results showed that exogenous Se positively decreased NO_3~- content and this effect was concentration-dependent. The lowest NO_3~- content was obtained under 0.5 μmol L–1 Se treatment. The application of Se enhanced photosynthetic capacity by increasing the photosynthesis rate(Pn), stomatal conductance(Cs) and the transpiration efficiency(Tr) of lettuce. The transportation and assimilation of NO_3~- and activities of nitrogen metabolism enzymes in lettuce were also analysed. The NO_3~- efflux in the lettuce roots was markedly increased, but the efflux of NO_3~- from the root to the shoot was decreased after treated with exogenous Se. Moreover, Se application stimulated NO_3~- assimilation by enhancing nitrate reductase(NR), nitrite reductase(Ni R), glutamine synthetase(GS) and glutamate synthase enzyme(GOGAT) activities. These results provide direct evidence that exogenous Se shows positive function on decreasing NO_3~- accumulation via regulating the transport and enhancing activities of nitrogen metabolism enzyme in lettuce. We suggested that 0.5 μmol L–1 Se can be used to reduce NO_3~- content and increase hydroponic lettuce yield.     

Study on Relationship Between Cucumber Germplasm and Propamocarb Residue Using Subjective Rating Technique

Propamocarb(PM)residue in cucumber(Cucumis sativus L.)receives little attention.As is well known to all,high PM residue of cucumber could lead to increase in the violation rates of maximum residue limits and ultimately cause harm to human health.Knowledge of PM residue could help cucumber breeders in developing cultivars with low PM residue and improving cucumber quality.In this study,32 representative cucumber accessions(26 breeding lines and six cultivars)from different regions of China were evaluated for their PM residue in fruit and leaf to provide meaning to the subjective rating,which was highly correlated with PM residual content of fruit(r=0.97)and leaf(r=0.94).In addition,PM residual content of North China ecotype was the highest and Pickling ecotype was the lowest in fruit and leaf of cucumber.The leaf had significantly higher(P<0.01)PM residual content than the fruit,and poor correlation between leaf and fruit was represented.This study verified PM residual relationship between fruit and leaf,and laid the foundation for further identification of germplasm resources and breeding of new varieties for low PM residue of cucumber.     

Effects of 1-methylcyclopropene and modified atmosphere packaging on fruit quality and superficial scald in Yali pears during storage

The Yali pear(Pyrus bretschneideri Rehd.) is susceptible to superficial scald during prolonged cold storage and at shelf life. This study investigated the effects of 1-methylcyclopropene(1-MCP) and modified atmosphere packaging(MAP) on changes of fruit quality and superficial scald during cold storage and at shelf life in Yali pear. Compared with MAP, the combination of MAP and 1-MCP(MAP+1-MCP) treatment reduced the carbon dioxide and ethylene content inside the packaging bag. The 1-MCP, MAP, and MAP+1-MCP treatments reduced the superficial scald index, malondialdehyde content, O_2~-· production rate and relative conductivity and inhibited the accumulation of α-farnesene and conjugated trienes in the peel. 1-MCP and MAP+1-MCP treatments maintained a higher phenolic content and enhanced the catalase and superoxide dismutase activities in the fruit, while reduced activities of lipoxygenase and polyphenol oxidase in the peel preceding the onset of superficial scald. Comprehensive analysis indicated that the MAP+1-MCP treatment is the most effective method tested for improving the quality of Yali pears during cold storage and at shelf life.     

Effects of collapsing gully erosion on soil qualities of farm fields in the hilly granitic region of South China

Collapsing gully erosion is a specific form of soil erosion types in the hilly granitic region of tropical and subtropical South China, and can result in extremely rapid water and soil loss. Knowledge of the soil physical and chemical properties of farmland influenced by collapsing gully erosion is important in understanding the development of soil quality. This study was conducted at the Wuli Watershed of the Tongcheng County, south of Hubei Province, China. The aim is to investigate soil physical and chemical properties of three soil layers(0–20, 20–40 and 40–60 cm) for two farmland types(paddy field and upland field) in three regions influenced by collapsing gully erosion. The three regions are described as follows: strongly influenced region(SIR), weakly influenced region(WIR) and non-influenced region(NIR). The results show that collapsing gully erosion significantly increased the soil gravel and sand content in paddy and upland fields, especially the surface soil in the SIR and WIR. In the 0–20 cm layer of the paddy field, the highest gravel content(250.94 g kg~(–1)) was in the SIR and the lowest(78.67 g kg~(–1)) was in the NIR, but in the upland filed, the surface soil(0–20 cm) of the SIR and the 40–60 cm soil layer for the NIR had the highest(177.13 g kg~(–1)) and the lowest(59.96 g kg~(–1)) values of gravel content, respectively. The distribution of gravel and sand decreased with depth in the three influenced regions, but silt and clay showed the inverse change. In the paddy field, the average of sand content decreased from 58.6(in the SIR) to 49.0%(in the NIR), but the silt content was in a reverse order, increasing from 27.9 to 36.9%, and the average of the clay content of three regions showed no significant variation(P0.05). But in the upland filed, the sand, silt and clay fluctuated in the NIR and the WIR. Soils in the paddy and upland field were highly acidic(p H5.2) in the SIR and WIR; moreover lower nutrient contents(soil organic matter(SOM), total N and available N, P, K) existed in the SIR. In the 0–20 cm soil layer of the paddy field, compared with the NIR and the WIR, collapsing gully erosion caused a very sharp decrease in the SOM and total N of the SIR(5.23 and 0.56 g kg~(–1), respectively). But in the surface soil(0–20 cm) of the upland field, the highest SOM, total N, available N, available P and available K occurred in the NIR, and the lowest ones were in the SIR. Compared with the NIR, the cationexchange capacity(CEC) in the SIR and WIR was found to be relatively lower. These results suggest that collapsing gully erosion seriously affect the soil physical and chemical properties of farmland, lead to coarse particles accumulation in the field and decrease p H and nutrient levels.     

Alleviation of Al Toxicity in Barley by Addition of Calcium

The potential mechanism by which Ca alleviates Al toxicity was investigated in barley seedlings. It was found that 100 Al-alone treatment inhibited barley plant growth and thereby reduced shoot height and root length, and dry weights of root, shoot and leaf; promoted Al accumulation but inhibited Ca absorption in plant tissues; and induced an increase in the activities of superoxide dismutase （SOD）, catalase （CAT）, and peroxidase （POD） and in the level of lipid peroxidation （MDA content） in leaves. Except for the increase in Ca concentration in plant tissues, treatment with 0.5 mM Ca in the absence of Al had less effect on the above-mentioned parameters, compared with the control. Addition of Ca efficiently reduced Al toxicity, which is reflected by the promotion of plant growth, reduction in Al concentration and MDA content, increase in Ca concentration and in SOD, POD, and CAT activities compared with the Al-alone-treatment; with increase in Ca level （3.0 raM）, the ameliorative effect became more dominant. This indicated that the alleviation of aluminum toxicity in barley seedlings with Ca supplementation could be associated with less absorption of Al and the enhancement of the protective ability of the cell because of increased activity of the antioxidative enzyme.     

Effect of Distances from Different Trees Species on the Properties of Mollic Horizon

The study area is located in Duhok province-Kurdistan region-Northern Iraq and including two locations the first location is situated in Galbook village and the vegetation cover is trees of （Quercus aegilops L.）, the second location is situated in Koradeer village that covered with threes of Prunus dulcis （Mill.） Webb.. Disturbed surface soil samples at depth 0-30 cm were collected at four different distances （1, 5, 10 and 15 m） from the tree with randomized selection three trees in each study location. Soil organic matter decreases with increasing distances from tree in both locations under two different trees species, which is attributed to the effect of tree crown. Ordinarily, organic matter content in both locations correspond to the requirements of Mollisols. Statistical analysis of data showed significant differences in organic matter content between locations. Cation exchange capacity is high under tree crown and decreasing with distances from tree in both locations, as a result of decreasing organic matter and increasing calcium carbonate with distances. Commonly soil pH is slightly increased with increasing distances from tree because of increasing calcium carbonate with distances and decreasing organic matter content. Calcium was not uniform with distances and Mg decreasing with distances in both locations. The values of fine clay/coarse clay and fine clay/total clay of soil samples confirm development of the soils under study. Porosity percentage under tree crown is high and decreasing with distances. Soil bulk density was increased with distances in both locations, as a result of decreasing organic matter content with distances. This study aims to explain the effect of tree distances and species on the mollic horizon properties.     

Effects of Different Cadmium Levels on Active Oxygen Metabolism and H2O2-Scavenging System in Brassica campestris L. ssp. chinensis

The effects of different Cd (Cadmium) levels on generation of active oxygen speceies (AOS) and H2O2-scavenging system in the leaves of Brassica campestris L. ssp. chinensis were studied. The results showed that O2 generation rate, and H2O2 content were enhanced and malondialdehyde (MDA) content increased with the increase of Cd concentrations in the growth medium. The activities of ascorbate peroxidase (APX), dehydroascorbate reductase (DR) and glutathione reductase (GR) were promoted by the addition of Cd. Exposed to Cd also increased the contents of ascorbate (AsA) and glutathione (GSH) in the leaves.     

Photosynthesis, Carbohydrate Storage and Remobilization During Grain Filling as Affected by Sulphur Application in Winter Wheat

Photosynthesis and carbohydrate storage and remobilization was investigated during grain filling of winter wheat (Triticum aestivum L., cv. Lumai 22) in sulphur application experiments where the available sulphur (S) content in the soil of 0-20 cm soil layer was 5.84 mg kg^-1. Two levels of S were applied as S0 (0 kg S ha^-1) and S1 (67.5 kg S ha^-1). The results showed that net photosynthetic rate of flag leaf of S1 treatment was significantly higher than S0 treatment, and it increased gradually from anthesis to 35 DAA. Sucrose-phosphate synthase (SPS) activity and sucrose content of S1 treatment were significantly higher than those of S0 treatment. The contents of total soluble sugar, sucrose, fructose, glucose, DP3 and DP≥4 fructan in stem and sheath of S1 treatment were significantly higher than those of S0 treatment also. The amount of total soluble sugar and fructans remobilization from stem and sheath and the starch accumulating rate in grain of S1 treatment was significantly higher than those of S0 treatment. It is suggested that sulphur application increase photosynthate remobilization from stem and sheath to grain at later filling stage.