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两个流行猪痢疾猪场的1095头猪,全群喂服痢菌净,结合环境和猪体消毒,未服药的小猪与净化后的母猪混群饲养3个月后,采肛拭作粪便分离培养,未检出猪痢疾密螺旋体。两处猪群已观察至19个月也未发生猪痢疾病猪。 相似文献
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猪痢疾是猪特有的一种肠道传染病,由致病性的猪痢疾短螺旋体引起,主要发生于仔猪,临床主要表现为腹泻、黏液性出血性下痢、消瘦等,病理学特征为局限于大肠的出血性、渗出性、纤维素性甚至坏死性结肠炎或盲肠炎。该病一旦发生,不易清除,可能会造成猪群的大量死亡,治愈后的猪生长发育较迟缓,生产力严重下降,给养猪业造成重大经济损失。从病原学和流行病学做出有效的诊断治疗和防控其发生发展,能够有效的减少经济损失。 相似文献
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猪痢疾是由猪痢疾密螺旋体引起的一种以黏液性出血性下痢为特征的肠道传染病。介绍了该病的发病情况、临床症状、剖检变化、诊断和防治措施。 相似文献
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对发生猪痢疾的5个猪场采用痢菌净注射或拌料口服,连用6d。接着以6.0×10~(-5)喹乙醇拌料口服,连用15d。用药期间,全场进行全面清扫、消毒,灭鼠、灭蝇,结果使5个猪场达到净化本病的要求。 相似文献
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猪瘟与高致病性蓝耳病混合感染的诊断及病原特性分析 总被引:1,自引:0,他引:1
[目的]诊断由猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)和猪伪狂犬病毒(PRV)引发的猪疫病,并分析病原。[方法]采集福建某猪场发病猪的组织器官,提取病毒DNA或RNA,PCR检测病毒感染。采用ELISA方法检测CSFV、PRRSV和PRV的感染情况。[结果]测序分析和ELISA结果表明,猪场存在PRRSV、CSFV、PRV3种病毒感染。[结论]该次猪病疫情主要是由CSFV和高致病性PRRSV混合感染引起。 相似文献
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阐述了猪痢疾与附红细胞体混合感染的临床症状、剖检病变及实验室检查方法,并从加强饲养管理及药物预防2个方面总结了该病的预防措施,最后提出相应的中西医结合治疗方法。 相似文献
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研究陕西省猪瘟病毒E2基因主要抗原区变异情况,为猪瘟防控提供依据.用巢式PCR方法对猪瘟疫苗和采自陕西省部分地区的疑似猪瘟病料进行E2基因主要抗原区扩增并测序,将测序结果与HCLV疫苗株和Shimen株E2基因进行序列比对分析.结果表明,23株流行毒株之间的核苷酸同源性在86.0%~100%,氨基酸同源性在87.8%~... 相似文献
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安徽省发生非洲猪瘟疫情以来,望江县严格按照省、市会议精神和相关文件要求,强化动物卫生监管措施,积极做好非洲猪瘟防控工作,取得了较好的成效。 相似文献
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闽西地区猪伪狂犬野毒株感染的血清学调查 总被引:3,自引:0,他引:3
美国IDEXX公司生产的PRV-gE(gE-ELISA)酶联免疫试剂盒,能对已免疫PR缺失gE的基因工程疫苗或未免疫的猪群,区分疫苗株和野毒株感染.2005~2007上半年,对闽西地区使用gE-基因缺失疫苗的规模化猪场母猪、仔猪和生长猪,不分健康状况,进行PR野毒株感染的血清学调查,结果显示2005年至2006年上半年,该地区母猪PR野毒感染阳性率较高,达41.5%和43.8%,2006年下半年至2007上半年,该地区母猪PR野毒感染阳性率有较大幅度的降低,且阴性猪场很多,阳性率分别是21.3%和13.3%.同时3年来对54个规模化猪场监测结果分析,各猪场阳性率差异很大,3年来猪场阳性率也呈大幅度下降,2007年上半年猪场阳性率仅为33.3%.对仔猪和生长猪的监测结果显示,阳性率比母猪大大降低,并且也呈下降的趋势. 相似文献
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A multiplex real-time PCR assay for simultaneous detection of classical swine fever virus,African swine fever virus,and atypical porcine pestivirus 下载免费PDF全文
SONG Xiang-peng XIA Ying-ju XU Lu ZHAO Jun-jie WANG Zhen ZHAO Qi-zu LIU Ye-bing ZHANG Qian-yi WANG Qin 《农业科学学报》2023,22(2):559-567
With the implementation of the C-strain vaccine, classical swine fever (CSF) has been under control in China, which is currently in a chronic atypical epidemic situation. African swine fever (ASF) emerged in China in 2018 and spread quickly across the country. It is presently occurring sporadically due to the lack of commercial vaccines and farmers’ increased awareness of biosafety. Atypical porcine pestivirus (APPV) was first detected in Guangdong Province, China, in 2016, which mainly harms piglets and has a local epidemic situation in southern China. These three diseases have similar clinical symptoms in pig herds, which cause considerable losses to the pig industry. They are difficult to be distinguished only by clinical diagnosis. Therefore, developing an early and accurate simultaneous detection and differential diagnosis of the diseases induced by these viruses is essential. In this study, three pairs of specific primers and Taq-man probes were designed from highly conserved genomic regions of CSFV (5´ UTR), African swine fever virus (ASFV) (B646L), and APPV (5´ UTR), followed by the optimization of reaction conditions to establish a multiplex real-time PCR detection assay. The results showed that the method did not cross-react with other swine pathogens (porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), foot-and-mouth disease virus (FMDV), pseudorabies virus (PRV), porcine parvovirus (PPV), and bovine viral diarrhea virus BVDV). The sensitivity results showed that CSFV, ASFV, and APPV could be detected as low as 1 copy mL–1; the repeatability results showed that the intra-assay and inter-assay coefficient of variation of ASFV, CSFV, and APPV was less than 1%. Twenty-two virus samples were detected by the multiplex real-time PCR, compared with national standard diagnostic and patented method assay for CSF (GB/T 27540–2011), ASF (GB/T 18648–2020), and APPV (CN108611442A), respectively. The sensitivity of this triple real-time PCR for CSFV, ASFV, and APPV was almost the same, and the compliance results were the same (100%). A total of 451 clinical samples were detected, and the results showed that the positive rates of CSFV, ASFV, and APPV were 0.22% (1/451), 1.3% (6/451), and 0% (0/451), respectively. This assay provides a valuale tool for rapid detection and accurate diagnosis of CSFV, ASFV, and APPV. 相似文献