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基于分子印迹技术的痕量分离方法正成为分析测试领域的研究热点,其关键技术是获得高亲和性和高选择性的分子印迹聚合物.目前,计算机模拟技术在分子印迹物制备过程中发挥着越来越大的作用.本文就计算机模拟在分子印迹聚合物制备中的应用现状和发展前景进行分析阐述,以期为食品、环境中有害物质的分子印迹聚合物的制备以及分子印迹技术领域的研究提供参考. 相似文献
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分子印迹聚合物具有高选择性、化学稳定性和制备简便的特点,广泛应用于农药残留检测中。本文概括了分子印迹技术基础理论,综述了分子印迹聚合物的制备及表征,分子印迹技术在农药残留检测中的应用,其中包括分子印迹在色谱固定相、固相萃取、固相微萃取、传感器等方面的应用,并对其发展趋势进行了展望。 相似文献
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张丽芬 《河北北方学院学报(自然科学版)》2005,21(3):13-17
分子印迹技术是近年来集高分子合成、分子设计、分子识别、仿生生物工程等众多学科优势发展起来的一门边缘学科分支.分子印迹聚合物由于具有与天然抗体同样的识别性能和与高分子同样的抗腐蚀性能的双重优点,因而广泛应用于生物工程、临床医学、环境监测、食品工业等众多领域.介绍了分子印迹技术的基本原理,对分子印迹聚合物合成研究的最新进展进行了综述,主要介绍了它在痕量分析中的应用. 相似文献
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《河北北方学院学报(自然科学版)》2021,37(3)
目的为了保证生鲜食品安全,提出基于分子印迹技术的生鲜食品化学污染物残留监测方法。方法采用边缘轮廓检测和特征聚类构建分子印迹成像模型,利用纹理检测方法对分子印迹信息进行增强处理,采用色谱分离方法与高分辨信息重组方法进行污染物残留内部分子结构信息检测,从而实现生鲜食品化学污染物残留监测。结果仿真结果表明,采用该方法进行生鲜食品化学污染物残留监测,各组份检测的准确性较高,污染物的浓度和含量等参数估计能力较好。结论该方法能够为生鲜食品安全提供充分保障,具有实际应用价值。 相似文献
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分子印迹技术在食品安全检测中的应用 总被引:1,自引:0,他引:1
分子印迹技术由于具有预定的选择性和高度的亲和性,在食品安全检测中具有广阔的应用前景.概述了分子印迹技术的起源与发展、原理及其在食品安全检测中的应用. 相似文献
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Greene E 《Science (New York, N.Y.)》1989,243(4891):643-646
Caterpillars of the spring brood of Nemoria arizonaria develop into mimics of the oak catkins upon which they feed. Caterpillars from the summer brood emerge after the catkins have fallen and they develop instead into mimics of oak twigs. This developmental polymorphism may be triggered by the concentration of defensive secondary compounds in the larval diet: all caterpillars raised on catkins, which are low in tannin, developed into catkin morphs; those raised on leaves, which are high in tannin, developed into twig morphs; most raised on artificial diets of catkins with elevated tannin concentrations developed into twig morphs. 相似文献
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[目的]快速筛选出番茄青枯病抗性基因的SSR标记。[方法]对来自4个国家27份番茄材料青枯病的抗性进行鉴定,并用一套SSR标记对材料进行分析,寻找与青枯病抗性基因连锁的标记。[结果]来自国内的3份材料表现出理想的抗病性,"湘引79-1"表现高抗,"860"及"039-3"表现抗病。标记TOM176-177在3份抗病材料上能扩增出相同带型,而在其他材料上扩增出的带型不同。TOM176-177在27份材料上存在4个等位基因,在3份抗病材料上是同一个等位基因,其扩增产物比其他等位基因小。[结论]该研究初步推断标记TOM176-177与青枯病抗性基因连锁。 相似文献
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A bacterial artificial chromosome (BAC) library is an invaluable resource tool to initiate tea plant genomics research, and the preparation of high molecular weight (HMW) genomic DNA is a crucial first step for constructing a BAC Library. In order to construct a BAC library for enhancing tea plant genomics research, a new method for the preparation of tea pant high molecular weight (HMW) genomic DNA must be developed due to young tea plant leaves and shoots are notably rich in both tea polyphenols and tea polysaccharides. In this paper, a modified method for preparing high quality tea plant HMW genomi~ DNA was optimized, and the quality of tea plant genomic DNA was evaluated. The results were as follows: Critical indicators of HMW DNA preparation were the appearance of the smooth nuclei in solution (as opposed to sticky-gummy) before agarose plug solidification, non-dark colored nuclei plugs after lysis with an SDS/proteinase K solution, and the quality and quantity of HMW DNA fragments after restriction enzyme digestion. Importantly, 1% dissolved PVP-40 and 1% un-dissolved PVP-40 during the nuclei extraction steps, in conjunction with the removal of PVP-40 from the plug washing and nuclei lysis steps, were critical for achieving HWM tea plant DNA suitable for BAC library construction. Additionally, a third PFGE fraction selection step to eliminate contaminating small DNA fragments. The modifications provided parameters that may have prevented deleterious interactions from tea polyphenols and tea polysaccharides. The HMW genomic DNA produced by this new modified method has been used to successfully construct a large-insert tea plant BAC library, and thus may be suitable for BAC library construction from other plant species that contain similarly interfering compounds. 相似文献
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以漳州水仙花主产区主栽品种"金盏"和"玉玲珑"为参照,利用IRAP标记技术对花变异体"金三角"及叶色变异体"金边水仙"进行变异分析.4对IRAP引物共获得177个位点,有123个多态性位点,4份中国水仙种质资源间的相似系数介于0.946-0.657之间.研究结果表明,变异体"金三角"和"金边水仙"与主栽品种"金盏"和"玉玲珑"的区别明显,转座插入和逆转座子间重组可能是引起中国水仙变异的重要机制之一. 相似文献
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分析了江南水乡的区位特征及其城乡一体化建设现状,指出江南水乡农民集中居住区规划设计中忽略了地域性的自然元素与人造元素的和谐结合,存在"水乡"特色缺失的问题,住区规划"城市小区"化、建筑风格"欧"化,针对这些问题,从规划、建筑与景观设计3个方面,就如何塑造"水乡"特色,提出了相关的规划设计思路与方法,着重探讨了建设基地类型、环境与住区建设相结合的重要性;当今住宅可从传统建筑中获取的灵感和设计方法;景观设计在总体规划和具体设计中的内容。 相似文献
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Although the concept of an artificial photosynthetic reaction center that mimics natural electron-and energy-transfer processes is an old one, in recent years major advances have occurred. In this review, some relatively simple molecular dyads that mimic certain aspects of photosynthetic electron transfer and singlet or triplet energy transfer are described. Dyads of this type have proven to be extremely useful for elucidating basic photochemical principles. In addition, their limitations, particularly in the area of temporal stabilization of electronic charge separation, have inspired the development of much more complex multicomponent molecular devices. The use of the basic principles of photoinitiated electron transfer to engineer desirable properties into the more complex species is exemplified. The multiple electrontransfer pathways available with these molecules make it possible to fine-tune the systems in ways that are impossible with simpler molecules. The study of these devices not only contributes to our understanding of natural photosynthesis, but also aids in the design of artificial solar energy harvesting systems and provides an entry into the nascent field of molecular electronics. 相似文献
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《Science (New York, N.Y.)》1988,241(4862):149
The address given for Small Talk/V, Digitalk, Inc., in reference 2 of the software review "PC sofotware for artificial intelligence applications" by Helmut Epp et al. (6 May, p. 824) was incorrect. It should have been 9841 Airport Boulevard, Los Angeles, CA 90045. 相似文献