共查询到19条相似文献,搜索用时 125 毫秒
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外源DNA处理小麦种苗和颖花及其诱导变异的研究初报 总被引:2,自引:0,他引:2
采用外源DNA导入技术,研究了DNA种苗浸渍对小麦种子发芽的影响,颖花滴注对结实率的影响以及外源DNA导入受体诱导变异的效果。结果表明,外源DNA浸种对发芽生根有不良的影响。浸种时DNA稀释液的浓度不应大于0.1×ssc。DNA液颖花滴注可获得较高的结实率。外源DNA导受体后产生了具有目的性状的变异(如粒色、芒长、不育性等)和非供体性状的变异(如株带蜡质、抽穗迟等)。而且大麦DNA导入小麦后,成功地获得了高抗白粉病变异株。 相似文献
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棉花分子育种研究的主要进展山东棉花研究中心董合忠植物分子育种包括两个层次的生物工程技术,一是外源DNA直接导入技术,即以整体植物的细胞为受体,将带有目的性状的供体DNA导入植物,筛选获得目的性状的后代;二是基因工程技术,将目的基因分离出来,经重组构建... 相似文献
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综述了DNA甲基化分子机制,小麦在胁迫作用、物理化学因素、外源基因导入时基因组DNA甲基化水平的变化以及小麦中甲基化转移酶的研究。 相似文献
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小麦外源基因导入的研究进展 总被引:5,自引:0,他引:5
远缘杂交是创造新物种、新种质以及转移异源遗传物质的有效途径,在理论和实践上都具有重要意义。本文综述了近年来小麦外源基因导入的研究进展概况,并根据我们多年的远缘杂交育种实践,综合分析了当地的生态气候条件,提出了生态环境与糖蛋白互作诱导远缘杂种育性假说。 相似文献
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授粉后外源DNA(基因)导入技术是实现作物基因转移的分子育种新途径。我们采用这一技术将抗白粉病的大麦DNA导入感病小麦品种(以二棱大麦品种80S—111为供体,小麦花培品种花76为受体)后产生多种变异类型。本文主要通过对后代5个抗白粉病稳定的株系的抗性表现、与已知基因的比较、籽粒氨基酸成分的变化 相似文献
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通过花粉管通道导入外源DNA创造抗白粉病小麦新种质 总被引:9,自引:0,他引:9
小麦品种复壮 3 0和小白冬麦的白粉病抗性表现突出 ,是 2个抗性持久、抗谱广泛的优良白粉病抗源。但由于农艺性状欠佳 ,不适于直接作为亲本用于常规育种。为此 ,采用花粉管通道法将复壮 3 0和小白冬麦的基因组DNA导入农艺性状较好的小麦品种北农 6号 ,旨在转移复壮 3 0和小白冬麦的抗白粉病基因 ,创造抗白粉病小麦新种质。本文总结 3年来所得到的研究结果 ,阐述采用花粉管通道法导入外源DNA创造新种质的可行性 相似文献
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离子束介导转大豆DNA小麦后代品质和农艺性状分析 总被引:1,自引:0,他引:1
利用离子束介导外源基因群转移的方法,分别将大豆全长DNA、DNA片段导入小麦,通过对M_1~M_3蛋白质含量和株高、粒质等农艺性状的变异分析可知,该方法能有效提高小麦蛋白质含量,降低株高,改变粒质。M_1获得蛋白质含量18%以上(较ck增加2.0个百分点)单株183个,M_2获得蛋白质含量18%以上(较ck增加3.6个百分点)单株33个,M_3获得蛋白质含量18%以上(较ck增加5.1个百分点)单株35个。M_3获得株高62cm以下(较ck降低7cm)的单株102个,占4.1%,部分单株株高能够稳定遗传。转基因后代部分单株和株系籽粒变为全角质。 相似文献
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基因枪法获得GNA转基因小麦植株的研究 总被引:27,自引:1,他引:27
以小麦品种京 411作为基因枪转化的靶材料 ,取其未成熟胚诱导愈伤组织 ,经过 10 d左右培养后 ,用含有雪花莲凝集素 (Galanthus nivalis agglutinin ,GNA)和 bar基因的质粒 p BI12 1- 2轰击 80 0个胚性愈伤组织 ,在含有4mg/ L Basta溶液的培养基上进行筛选 ,分化及生根培养 ,获得 6 7棵再生植株。田间涂抹 Basta溶液 (5 0、75 m g/ L)检测和接麦蚜实验 ,提取转基因植株基因组 DNA,用扩增 GNA基因的引物经 PCR扩增 Southern杂交 ,结果表明利用基因枪转化已从 T2 代获得了 8株含有编码 bar/ GNA基因的转基因植株。 相似文献
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1 《中国农学通报》是中国农学会主办,由两院院士、著名农业科学家石元春先生任主编,国内外公开发行的国家级农业学术期刊(每月5日发行)。也是国家科技部"中国科技核心期刊"、中国科协优秀学术期刊和全国优秀农业期刊。2 本刊特点:以中青年学科带头人和博士、硕士为主要作者群,以省部级以上科研课题或基金项目论文为刊文重点,出版周期短,载文容量大。 相似文献
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高粱DNA导入小麦后代的和面仪曲线变化 总被引:5,自引:0,他引:5
高粱外源基因导入小麦后代的面团流变学特性有较大的变化,和面时间、峰高和七分钟尾高均超过或低于受体的后代,而且因受体不同表现有所差异;后代的七分钟带宽不同程度地大于受体;外源DNA导入小麦引起品质的变化,为选育高产、优质的新品种(系)提供了机会。 相似文献
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[目的]将磷酸烯醇式丙酮酸羧化酶(PEPCase)基因导入普通小麦临优145。[方法]利用根癌农杆菌遗传转化系统,以普通小麦临优145为材料,将磷酸烯醇式丙酮酸羧化酶(PEPCase)基因,导入小麦胚性愈伤组织中,用含潮霉素(hyg)的筛选培养基连续筛选,并从分子水平上检测该基因。[结果]获得了hyg抗性植株,经GUS组织化学染色检测表明,抗性苗叶片被染成了深蓝色;PCR检测出目标条带。[结论]初步证明磷酸烯醇式丙酮酸羧化酶(PEPCase)基因已经导入受体材料中。 相似文献
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WANG Song-wen SHI Li-li SUN Zong-xiu CAI Bao-Li FU Ya-ping WANG Yang SI Hua-min LIU Xia ZHANG Xin 《中国农业科学(英文版)》2005,4(4)
Atrazine chlorohydrolase gene (atzA) was cloned from Arthrobacter sp. AD1. A plant expression plasmid was constructed under the control of CaMV35s promoter and was used in rice transformation. The target gene was successfully introduced into mature embryos of a japonica rice cultivar Jindao 107 by Agrobacterium- mediated transformation and hundreds of transgenic plants were obtained. The exogenous atzA gene in the transgenic plants that expressed atrazine resistance was confirmed by Southern blot hybridization. The resistance experiments by spraying transgenic rice plants with 0.133% atrazine shown that most of the transgenic rice plants exhibited the resistance to herbicide atrazine. The segregation of exogenous atzA gene in T1 progeny corresponded to the Mendelian ratio. 相似文献
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PANG Jun-lan XU Hui-jun DU Li-Pu YE Xing-guo LI Lian-cheng XIN Zhi-yong MA You-Zhi DIAO Ai-po Adams M J 《中国农业科学(英文版)》2003,2(4)
CWMV-CP1 target gene and bar selection gene were co-transferred into commercial wheat vari-ety of Yangmai158 by particle bombardment. In total, 145 resistant plants to 3 - 5 mg L-1 Bialaphos were ob-tained, 21 plants were identified to be positive in T0 generation by PCR-Southern test, and the transformationfrequency had 0. 99%. T1 plants were further tested by PCR and Southern hybridization. Results demonstra-ted that the alien resistance gene had been integrated into the wheat genome. The segregation ratio of CP1+ toCP1- in T1 generation was 1.0 to 1.3, and didn't agree with Mendelian rule. RT-PCR result from T2 plantsshowed that the alien gene CWMV-CP1 had stable expression in wheat genetic background. 相似文献
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Wheat is the number one crop both in acreage and total yield in the world. Therefore, it is very important to improve wheat by gene engineering techniques even though it belongs to the plants insensitive to gene transformation, especially to Agrobacterium-mediated transformation. Wheat immature embryos of 1.0-1.5mm in size, C58c1 of Agrobacterium strain harboring pPTN249, pPTN270, pPTN254, and pSIS-GFP respectively (all the vectors contain the aphA selectable gene driven by enhanced 35S promoter and a target gene controlled by ubi promoter or E35S promoter), AB medium for Agrobacterium activate culture, WCC medium for co-culture, were used in this study. The embryos with 4 days of pre-culture were transferred onto selection medium with 10mg/L geneticin, 50mg/L ticarcillin, 50mg/L vancomycin, and 50mg/L cefotaxine after 30 minutes of infection and 2 days of co-cultivation with Agrobacterium. Followed callus production,shoot regeneration on selection medium, 114 resistant plantlets were obtained from 10 transformation experiments of four genotypes. By nptⅡ ELISA (nptⅡ enzyme assay), PCR, Southern blot and leaf bleach,29 positive plants were identified from two genotypes of Bobwhite and Yanglmai 10, with an average transformation efficiency of 0.82 %. The result tested by Southern blot also showed that the transgenic plants with single- copy integration of target gene took 65.52% among total positive plants. The ELISA value of transgenic plant was also related to the copies of alien DNA integrating into wheat chromosomes, the transgenic plants with single copy integration giving higher ELISA value than the ones with 2 or 3 copies integration. 相似文献