高潜水位煤矿区完整复垦周期的土壤碳演变特征

在济宁高潜水位矿区的充填复垦区内,分析完整复垦周期的土壤碳演变特征。通过从塌陷地(M1)、复垦年限为0,3年的复垦耕地(M2、M3)以及正常耕地(M4)采集土样并检测土壤全碳(TC)、有机碳(SOC)、有机碳密度(SOCD)、有机碳组分(WDOC、POC、MOC、LFOC、HFOC、MBC)及稳定性同位素(δ13C),研究不同复垦周期阶段下土壤碳空间特征的变化情况。研究表明:(1)塌陷地的TC、SOC、SOCD和各有机碳组分含量较低。经过复垦措施期后,SOCD在复垦当年就恢复到正常耕地的水平,TC和SOC含量随着复垦年限不断增加。WDOC、POC、LFOC、MBC等组分恢复速率较快,MOC和HFOC恢复周期较长。(2)TC、SOC、SOCD和各有机碳组分的恢复速率随着土层深度的增加逐渐减缓。(3)复垦土壤的δ13 C均值在不同深度间的差异较小,而标准差和变异系数偏大,说明复垦土壤层次不明显且同一层次间土壤来源混乱。从整个复垦周期来看,复垦工作虽具有"碳汇"作用,但会对土壤层次造成破坏。建议进行塌陷地治理时,尽量采用对土层扰动较小的复垦方式,对表土剥离和回填工序进行优化和监督,从而达到更好的恢复效果。     

不同利用方式黑钙土有机碳组分剖面分布特征

为研究土地利用方式对土壤碳库的影响,以东北黑钙土区的天然草地、人工林地和耕地为研究对象,采集0—100 cm土体中不同土层样品(A、AB、B_k、BC和C层),测定不同利用方式土壤有机碳(SOC)、水溶性有机碳(WSOC)、轻组有机碳(LFOC)和重组有机碳(HFOC)含量。结果表明:3种利用方式SOC、LFOC和HFOC主要分布在A层,但草地WSOC含量在B_k层最多(0.27 g/kg)。由A~C层,3种土地利用方式土壤有机碳及组分有机碳含量总体均呈减少趋势,但减少程度明显不同,天然草地缓慢减少,人工林地急剧减少,耕地逐渐减少。草地转换为林地和开垦为耕地后,均造成土壤有机碳及组分有机碳含量减少,WSOC减少34%和48%,LFOC减少20%和37%,HFOC减少7%和5%,SOC减少10%和16%。草地转换为林地和开垦为耕地后,WSOC/SOC、LFOC/SOC显著降低,但HFOC/SOC却提高,说明草地被开垦后活性有机碳含量快速下降。土地利用方式和土层对SOC、WSOC和LFOC具有显著影响,且对SOC和HFOC叠加效应较强。同时,土壤理化性质也在一定程度上影响着SOC、WSOC、LFOC和HFOC。应制定合理土地利用管理政策,保护自然草地免遭破坏,减少土壤有机碳流失,发挥草地生态系统碳固存的重要作用。     

岩溶区不同土地利用方式对土壤有机碳及其组分的影响

选择重庆市中梁山岩溶槽谷中5种土地利用方式(弃耕地、草地、菜地、橘园地和林地)为研究对象,在野外调查和室内分析的基础上,采用方差分析法对比分析了土壤总有机碳、溶解性有机碳、易氧化性碳、轻组有机碳、颗粒有机碳及矿物结合态有机碳的含量变化,并用相关分析法分析了土壤有机碳各组分之间的关系。结果表明:不同土地利用方式土壤总有机碳(TOC)含量随土层深度增加而降低,上下层土壤TOC含量在2.69~13.88g/kg之间,不同植被覆盖类型、耕作方式和施肥是影响土壤TOC分布的重要因素。不同土地利用方式下有机碳各组分(DOC、EOC、LFOC、POC和MOC)含量在垂直分布上均呈现出随土壤深度的增加而降低的趋势,但不同土地利用方式之间的差异较大,主要与植被类型、施肥管理、耕作方式以及人为干扰有关。弃耕地含量均比较低,林地和草地受人为干扰较少含量较高,菜地土壤受施肥和翻耕影响,上下层土壤含量差异不大,橘园地上下层土壤5种有机碳组分含量差异均是最大的。弃耕地受之前耕作影响,弃耕时间短,有机质输入量少,POC/MOC值相对较低,菜地受施肥和翻耕影响上下层土壤POC/MOC值相对比较稳定,草地、橘园地和林地0—20cm土壤POC/MOC值均高于20—40cm,下层土壤有机碳比较稳定。0—20cm和20—40cm土壤有机碳及组分之间存在相关关系,0—20cm土壤TOC与LFOC和MOC之间呈显著正相关,LFOC与POC之间也呈现显著正相关;20—40cm土层,土壤TOC与EOC、LFOC和MOC之间呈显著正相关性,MOC与TOC以及EOC和LFOC均呈现正相关性,LFOC与POC之间关系由表土层的显著正相关转变为极显著正相关。下层土壤比上层土壤有机碳稳定性强,尤其是土壤TOC、LFOC和MOC能够敏感地反映土壤碳库的变化,可以作为土壤有机碳稳定性的敏感性指标。     

不同利用方式对棕壤有机碳垂直分布的影响

采用野外采样和室内分析的方法研究了林地、园地、耕地3种利用方式对典型棕壤总有机碳(TOC)、颗粒有机碳(POC)及重组有机碳(HFOC)在0～20 cm、20～40 cm、40～60 cm 3层次中垂直分布的影响。结果表明,与林地相比,园地和耕地各层次的TOC含量和储量均显著下降;其分布份额和分布比则为园地中、下层略向上层转移,耕地则明显向中、下层转移。3种利用方式下POC的相对数量均随土层加深而递减,林地开垦为园地和耕地后,POC的相对数量仅在园地上、中层显著降低,分别减少6.67和1.70个百分点,而耕地则各层次均显著降低,其相对数量分别减少13.65、5.43和3.03个百分点;HFOC的相对数量随干预强度和土层深度增加而增大,耕地和园地比林地分别高出:上层5.77和4.00个百分点、中层10.44和6.40个百分点、下层7.35和3.92个百分点,且差异均显著。因此,将林地棕壤开垦为园地或耕地后应注重有机物料的投入,以减缓因开垦对有机碳所造成的损失和不尽合理的分布状况。     

岩溶山地不同土地利用方式土壤颗粒有机碳和矿物结合态有机碳的分布特征

对重庆中梁山岩溶山地不同土地利用方式下0-40cm土壤颗粒有机碳和矿物结合态有机碳的含量和分布特征进行了研究.结果表明:不同土地利用方式土壤有机碳含量平均值表现为:林地>菜地>草地>橘园地>弃耕地.除橘园地外,其它各土地利用类型土壤细颗粒有机碳(FPOC)含量大于粗颗粒有机碳(CPOC).不同利用方式土壤颗粒有机碳含量在剖面层次中表现不同.0-20cm表层土壤CPOC含量表现为:橘园地>草地>菜地>林地>弃耕地,差异较大.土壤FPOC含量表现为:林地>草地>菜地>橘园地>弃耕地;20-40cm土壤CPOC和FPOC最高值出现在菜地,最低值出现在弃耕地.不同土地利用方式土壤矿物结合态有机碳(MOC)含量和土壤有机碳含量分布特征一致.除橘园地外土壤各组分有机碳分配比例大致表现为:MOC/SOC>CPOC/SOC>FPOC/SOC.相关分析表明,不同土地利用方式土壤SOC和POC呈正相关,相关性不一致.林地和草地呈极显著相关(P<0.01),弃耕地呈显著相关(P<0.05),菜地和橘园地相关性不显著.表明人为干扰和耕作措施会影响POC对SOC的贡献.     

亚热带森林转换对不同粒径土壤有机碳的影响

以亚热带天然阔叶林和由其转换而来的针阔混交人工林和杉木人工林为研究对象,探讨森林转换对土壤有机碳(Soilorganiccarbon,SOC)含量和分布格局的影响。选取不同土层(0～20cm、20～40cm、40～60cm)土壤作为样本,运用物理分组方法研究森林转换对土壤粗颗粒有机碳(Coarse particulate organic carbon,CPOC)、细颗粒有机碳(Fine particulate organic carbon,FPOC)、矿物结合态有机碳(Mineral-associatedorganiccarbon,MOC)含量及其分配比例的影响。结果表明:天然林转换为人工林后(1)各土层土壤有机碳含量均呈下降趋势;(2)0～20cm土层土壤粗颗粒有机碳含量和分配比例均显著降低,土壤细颗粒有机碳含量和比例呈增加趋势;矿物结合态有机碳含量呈减少趋势,比例呈增加趋势;(3)各土层土壤颗粒有机碳/矿物结合态有机碳(POC/MOC)和矿物结合态有机碳/土壤有机碳(MOC/SOC)比值均呈下降趋势,0～20 cm土层土壤CPOC与SOC相关性最好,40～60cm土层MOC与SOC相关性最好。因此,亚热带天然阔叶林转换为针阔混交人工林和杉木人工林,土壤总有机碳含量降低,土壤有机碳的稳定性增强;土壤CPOC更能反映森林转换对表层土壤有机碳的影响;而MOC更能反映森林转换对深层土壤有机碳的影响。     

黄土台塬不同土地利用方式下土壤碳组分的差异

为探讨土地利用方式对土壤碳固定的影响,以乔木、灌木、草和农田等不同植被类型,纯林和混交两种栽培模式的黄土台塬为对象,进行了土壤碳组分研究。结果表明,不同利用方式下林地和天然草地在0—100 cm土层总碳,轻组、重组、可溶性有机碳以及轻组有机碳分配比例(LFOC/SOC)均不同程度高于耕地,而其有机无机复合度(HFOC/SOC)则低于耕地,灌木林地和天然草地这种趋势尤为突出;各种土地利用方式间,土壤总碳和HFOC/SOC在0—20cm差异显著,总碳在60—100 cm也差异明显,轻组、重组及可溶性有机碳在0—40 cm,而无机碳则在40—100 cm差异明显;LFOC/SOC和DOC/SOC在各土层均存在一定差异。土壤总碳、有机碳以及各组分有机碳之间呈极显著正相关,而无机碳则与其呈负相关。轻组和可溶性有机碳均与粗颗粒、易氧化有机碳以及2—0.25 mm团聚体有机碳的相关性高于与细颗粒、稳态有机碳和2 mm团聚体有机碳;而重组有机碳则与之相反。轻组有机碳较有机碳、总碳、重组以及可溶性有机碳能更敏感地反映利用方式之间的差异,可作为土壤质量变化的评价指标。     

荒漠草原沙漠化对土壤无机碳和有机碳的影响

以空间代替时间的方法,通过对宁夏荒漠草原不同沙漠化阶段土壤有机碳(SOC)和无机碳(SIC)的研究,探讨荒漠草原沙漠化对土壤SIC、SOC及不同粒径组分土壤SIC、SOC分布特征的影响。结果表明:(1)随着荒漠草原沙漠化程度的加剧,0—10cm土层各粒径组分土壤SIC和SOC含量呈下降趋势。半固定沙地和流动沙地各粒径组分土壤SIC含量均表现为黏粉粒无机碳(CSIC)>细砂粒无机碳(FIC)>粗砂粒无机碳(CIC),而SOC含量均表现为细砂粒有机碳(FOC)>粗砂粒有机碳(COC)>黏粉粒有机碳(CSOC)。(2)随着荒漠草原沙漠化程度的加剧,0—30cm土层土壤无机碳(SICD)、土壤有机碳(SOCD)和土壤总碳(STCD)密度均表现为荒漠草原>固定沙地>半固定沙地>流动沙地。固定沙地、半固定沙地和流动沙地土壤SOCD、SICD分别比荒漠草原降低了18.5%,57.7%,60.5%和6.7%,35.9%,47.0%。(3)0—10cm土层各粒径组分土壤SOC和SIC含量、全土SOC含量与0—30cm土层SOC和SIC均呈显著正相关关系,其中土壤粗砂粒有机碳和粗砂粒无机碳对SOC影响最大,而土壤黏粉粒有机碳和黏粉粒无机碳与全土SIC含量呈显著负相关关系。因此,沙漠化防治对于减少荒漠草原土壤碳损失极为重要。     

黄土高原人工沙棘林恢复阶段土壤黑碳、颗粒有机碳的积累

对黄土高原沟壑区人工沙棘林不同恢复阶段土壤中的黑碳(BC)、总有机碳(TOC)、活性有机碳(LOC)和颗粒有机碳(POC)、稳定性有机碳(SOC)、矿质结合态有机碳(MOC)的空间分布和积累进行研究.结果表明,不同恢复阶段人工沙棘林的土壤BC、POC、LOC、TOC由表层至下层均呈显著降低规律,SOC、MOC由表层至下层均呈显著上升规律.不同恢复阶段0-10 cm土层表现为MOC＞ SOC＞ BC＞POC＞ LOC,10-20 cm土层表现为SOC＞ MOC＞ BC＞ POC＞ LOC,20-30 cm土层表现为SOC＞ MOC＞BC＞ POC＞ LOC.不同恢复阶段人工沙棘林土壤BC/TOC以0-10 cm土层最低,随恢复阶段的延长而下降.不同恢复阶段LOC/TOC、POC/TOC由表层至下层呈明显降低规律.不同恢复阶段在0-10 cm,10一20 cm土层中,BC/TOC随恢复年限延长呈下降趋势,而POC/TOC呈上升趋势,恢复年限对LOC/TOC均无影响,且两土层呈现一致的规律.20-30 cm土层中,恢复年限对POC/TOC无影响.BC/TOC随恢复年限延长呈明显下降趋势,LOC/TOC则呈现凹形分布.     

滇中尖山河小流域不同土地利用类型土壤活性有机碳分布特征

土壤活性有机碳对土地利用方式最为敏感,定量分析不同土地利用方式对土壤活性有机碳分布特征的影响对流域的土壤碳循环研究具有重要意义。从滇中尖山河小流域坡耕地、荒草地、林地、园地4种不同土地利用类型角度,系统地分析了0—10,10—20,20—30 cm土层土壤有机碳（SOC）、微生物有机碳（MBC）、易氧化有机碳（EOC）及可溶性有机碳（DOC）的分布特征及其相关性。结果表明:不同土地利用类型下土壤SOC,MBC,EOC,DOC整体均表现为园地 > 林地 > 坡耕地 > 荒草地;4种土地利用类型MBC,EOC,DOC整体上随着土层深度的增加而逐渐降低,且主要分布在0—20 cm土层,在20—30 cm土层含量较低（低于30%）;4种土地利用类型下SOC和MBC,EOC,DOC呈极显著正相关关系,MBC,EOC,DOC两两之间也表现出极显著正相关。综上,退耕还林以及在荒草地种植人工林可作为提高土壤有机碳及活性有机碳含量的有效措施,并将在减少流域水土流失和面源污染、改善土壤质量、恢复土壤肥力等方面起到重要作用。     

Soil-profile distribution of primary and secondary plant-available nutrients under conventional and no tillage

Nutrient distributions under no tillage (NT) compared with conventional disk-and-bed tillage (CT) management in the warm, humid region of the southeastern USA need to be assessed so that future placement, quantity, and type of fertilizers can be altered, if necessary, to efficiently match crop demands. We determined soil-profile distributions of pH, N, P, S, K, Ca, Mg, Na, Zn, Fe, Mn, and Cu to a depth of 0.9 m at the end of 8.5 years of continuous CT and NT management on a Weswood silty clay loam (fine, mixed, thermic Fluventic Ustochrept) in southcentral Texas. Most dramatic changes occurred within the 0–0.05 m depth, where soil under NT had lower pH, Fe, and Cu than under CT, but greater P, K, Zn, and Mn. Greater P and K under NT than under CT also occurred below the till-zone (0.15–0.3 m). At a depth of 0–0.3 m, soil under NT contained greater amounts of extractable P, K, Zn, Fe, Mn, and Cu than under CT. Nitrogen fertilization had little effect on nutrient distributions, except resulting in greater extractable K at 0–0.05 m and greater nitrate at 0–0.15 m. Few changes in soil-profile distributions were observed for extractable S, Ca, Mg, and Na. Long-term continuous use of NT on this fine-textured, high-fertility (except for N) soil had no apparent adverse effects on nutrient distributions relative to CT, but enhanced conservation and availability of P, K, Zn, Fe, Mn, and Cu near the soil surface where crop roots proliferate.     

Assessing the effectiveness of conservation measures: Resolving the “wicked” problem of the Steller sea lion

“Wicked” problems are those that are complex and that change when solutions are applied. Many conflicts in conservation fall into this category. The study approached the problem of how to constrain the apparent wickedness of a problem in the conservation management of a species by using simple empirical indicators to carry out iterative assessment of the risk to a population and to document how this risk evolves in relation to the addition of new data and the implementation of management actions. Effects of high levels of uncertainty within data and also concerning population structure were examined through stochastic simulation and by exploration of scenarios. Historical trends in the example used, the Steller sea lion, showed rapid declines in abundance in some regions during the 1980s. The current total population is 130,000-150,000 Steller sea lions through Alaska and British Columbia and this number has been stable since about 1990 in spite of regional differences in population dynamics. Regional differences in the sequence of changes in the number of pups and non-pups, suggested that an internal re-distribution of juveniles could have happened between 1980 and 1990. Current productivity also appears close to the long-term mean. Stochastic population projection using various scenarios showed that, based upon this history, the risk of extinction for the population has declined and is below reasonable thresholds for considering the population to be endangered. The trends in risk suggest that management actions taken since 1990 have probably been effective. Consequently, the conservation management objectives for the Steller sea lion are probably being met. The approach provides a mechanism, based upon experience and scenario analysis, for exploring future policy options and may help to constrain the debate amongst stakeholders about the cost-benefit trade-offs associated with different options.     

Enhanced establishment and growth of giant cardon cactus in an eroded field in the Sonoran Desert using native legume trees as nurse plants aided by plant growth-promoting microorganisms and compost

To evaluate the feasibility of long-term desert reforestation technology of mixed vegetation, cardon cactus (Pachycereus pringlei) seedlings from indoor and outdoor nurseries were planted in the field adjacent to one seedling of potential legume nurse trees: mesquite amargo (Prosopis articulata), yellow palo verde (Parkinsonia microphylla), and blue palo verde (Parkinsonia florida). Some of the planting holes were also supplemented with common dairy compost. Additionally, the combinations of legume tree–cactus were inoculated with either a consortium of desert arbuscular mycorrhizal (AM) fungi, plant growth promoting bacteria (PGPB; the diazotroph Azospirillum brasilense Cd, and the phosphate solubilizer Paenibacillus sp.), or a mixture of all. The field experiments were evaluated periodically during 30 months for survival and growth. Cardons reared in an outdoor screen house survived better in the field than those reared in a controlled growth chamber and hardened later outdoors. Association with any legume nurse tree increased survival and enhanced growth of untreated cardons. For cardons growing alone, application of either compost, AM fungi, and all the treatments combined increased survival. For these plants, no treatment affected plant growth during the first 3 months after transplanting. Later, all treatments, except for AM fungi, enhanced plant growth. However, only 2 years after transplanting the enhanced growth effect of AM fungi was also significant. In the presence of the legume nurse trees, transient positive effects on cardon growth were recorded. General evaluation after 30 months of cultivation showed that the treatments positively affected cardon growth when growing alone or in combination only with mesquite amargo but not with the other two legume trees. This study proposes that young legume trees have the capacity to enhance survival and growth of cardon cactus, depending on the legume cactus combination. Additional treatments such as compost or PGPB can either amplify the effect or else attenuate it.     

Karyotypic and genome size variation in Cajanus cajan (L.) Millsp. (pigeonpea) and some wild relatives

Detailed karyotypes and 4C DNA amounts have been studied in five cultivars of Cajanus cajan and 20 species belonging to Cajanus, Rhynchosia, Dunbaria, Flemingia and Paracalyx. C. cajan shows intraspecific variability and its karyotype is most similar to that of C. cajanifolius (sect. Cajanus) in the morphology and number of satellite chromosomes and the lack of any correlation between chromosome size within the complement and asymmetry. Karyotypes of C. lineatus and C. sericeus belonging to sect. Atylia are similar with respect to maximum r-index and the ratio of longest and shortest chromosomes in their respective complements. C. acutifolius (sect. Frutocosa) is distinct in having a very low ratio (1.44) between the longest and the shortest chromosomes, while in C. albicans, C. goensis, C. scarabaeoides (sect. Cantharospermum) there is no chromosome pair with r-index >2.0. C. mollis and C. volubilis (sect. Volubilis) show similarity with regard to ratio between the longest and the shortest chromosomes and a distinct chromosome pair with an arm ratio of 2.7 and C. platycarpus is distinct (sect. Rhynchosoides) in having the smallest ratio (1.36) between the longest and the shortest chromosomes. 4C DNA amounts in Cajanus and Rhynchosia vary between 3.28 pg to 11.69 pg and 4.92 pg to 11.13 pg respectively, while in Dunbaria, Flemingia and Paracalyx these vary between 4.70 and 7:06 pg. In the genus Cajanus karyotypic features and 4C DNA amounts agree with the sectional classification and bring out a close relationship between C. cajan and C. cajanifolius. This is supported by studies based on seed protein patterns, isozyme analysis, trypsin and chymotrypsin inhibitor patterns, RFLP and RAPD data and crossability relationships. A clear relation between DNA amount and abnormalities effecting the extent of pairing and recombination in interspecific hybrids shows the importance of this study in developing future breeding programme involving C. cajan and its wild relatives that are of potential value.     

Effect of some fungicide seed treatments on the survival of Bradyrhizobium japonicum and on the nodulation and yield of soybean [Glycine max. (L) Merr.]

Several commercial fungicide seed treatments were evaluated for their possible effect on the survival of Bradyrhizobium japonicum on seeds and on the nodulation and yield of soybeans in a greenhouse and a field experiment. quinolate Pro (carbendazim and oxine copper), Vitavax 200FF (carboxin and thiram), and Monceren (pencycuron) had a small effect or no effect on the survival of B. japonicum and on the nodulation and yield of soybeans. They can thus be considered compatible with soybean seed inoculation. Germipro UFB (carbendazim and iprodione), Apron 35J (metalaxyl), and Tachigaren (hymexazol) decreased B. japonicum survival and the nodulation and yield of soybeans and thus cannot be considered compatible with soybean seed inoculation.     

Einfluss der düngungsmethoden auf die entwicklung von zuckerrübenkrankheiten

Während neunjähriger (1994-2002) Untersuchungen in der Versuchsanstalt Ba?cyny (Fahlerde, Fall-Gleyboden, ausgebildet aus leichtem Lehmboden) wurde der Befall mit Krankheiten an der Zuckerrübe in Abhängigkeit von der Düngungsmethode bewertet. Folgende Methoden wurden angewendet: A - Mineraldünger, B - Stalldung und Mineraldünger, C - Stroh-, Gründung und Mineraldünger, D - Kompostdünger und Biopräparate (ökologische Düngung). Auf den Objekten A, B, C wurden bei konstanter Phosphor-Kalium-Düngung die Stickstoffgaben von 0 bis 180 kg.ha^?1 differenziert. Auf dem ökologischen Objekt D ohne Mineraldünger wurden statt Stickstoff die Biopräparate P₅₀₀ und P₅₀₁ verabreicht. Der geringste Befall von Krankheiten wurde an der Zuckerrübe auf jenem Objekt festgestellt, das mit Kompost und den Biopräparaten gedüngt worden war. Im Rahmen der konventionellen Düngungsmethoden wurde allgemein die höchste Intensität der Krankheiten an der mit Stroh, Gründung und Mineraldünger gedüngten Zuckerrübe beobachtet. Ausnahmen von dieser Regel bildete das Vorkommen von echtem Mehltau und Keimlingsfäule. Auf den Objekten A, B, C nahm die Intensität der Krankheiten mit der Steigerung der Stickstoffgabe zu. Auch die auf Objekt D verabreichten Biopräparate stimulierten die Entwicklung der Krankheiten. The effect of various fertilization methods on the diseases of sugar beet was estimated on the basis of nine-year studies (1994-2002) conducted in the Experimental Production Farm in Ba?cyny on loess-based, sedimentary-gley, light-clay soil. The following fertilization methods were applied: A - mineral fertilization, B - mineral and manure fertilization, C - straw, green manure and mineral fertilization, D - ecological compost and biodynamic preparations fertilization. In the objects A, B and C fertilized with phosphorus and potassium, various doses of nitrogen ranging from 0 to 180 kg/ha^?1 were used. In the ecological object D, where mineral fertilization was not used, biodynamic preparations P₅₀₀ and P₅₀₁ were applied instead of nitrogen. The lowest ratio of the diseases of sugar beet was observed in the object fertilized with the compost and biodynamic preparations. In view of the conventional fertilization methods, generally the highest incidence of diseases was noted in the sugar beet fertilized with straw, green manure and mineral fertilizers. The exception was the occurrence of powdery mildew and the black leg. In objects A, B and C the incidence of the diseases increased with the increase in nitrogen doses. The applied biodynamical preparations in the object D stimulated the development of diseases too.     

Prospects for control of Pythium damping-off of lettuce with Trichoderma,Gliocladium and Enterobacter spp.

Summary Damping-off of lettuce (Lactuca sativa) caused by Pythium ultimum was studied in pots containing a non-sterile potting mix in the glasshouse. Fifty P. ultimum sporangia g^-1 compost reduced the plant stand to 15% and shoot dry weight to 18%, but this reduction was totally prevented by applying Trichoderma harzianum at 2×10⁵ viable propagules g^-1 potting mix. Gliocladium virens and Enterobacter cloacae also alleviated the damping-off. E. cloacae did not adversely affect the action of the fungal antagonists. The co-existence of the bacterial and fungal antagonists was revealed on the root surface and inner surface of the testa by scanning electron microscopy, indicating the compatibility of the biocontrol agents.     

细菌、真菌及植物氮营养信号研究进展

氮素吸收代谢是所有生物生命活动的核心部分,因此,各种生物对氮的吸收与同化过程都有精细的调节。这种调节依赖于生物对体内氮素状况信号及体外（生长介质中）氮素信号的感受。过去几十年中,在细菌、真菌中已经对氮素营养信号有了较好的研究,而植物中则相对缓慢,但也有了一定的认识。本文重点比较细菌、真菌及植物中氮信号系统的组成,以期为进一步认识植物中的氮信号接受与转导系统提供启示。     

Aircraft operations near concentrations of birds in Antarctica: The development of practical guidelines

Aircraft operations have the potential to disturb and to impact negatively on bird life. A gradient of increasing behavioural response is evident in birds when exposed to increasing aircraft stimulus. The most major disturbance is likely to lead to impacts on the health, breeding performance and survival of individual birds, and perhaps bird colonies. A process of revision to policies on aircraft operations contained in management plans for a number of specially protected areas in Antarctica by the United Kingdom, accompanied by consultations made within the scientific community through the Scientific Committee on Antarctic Research (SCAR) and with operational interests through the Council of Managers of National Antarctic Programmes (COMNAP) resulted in new guidelines being adopted by the Antarctic Treaty Consultative Parties in June 2004. The principal recommendations of the guidelines are that bird colonies should not be overflown below 2000 ft (∼610 m) above ground level and landings within 1/2 nautical mile (∼930 m) of bird colonies should be avoided wherever possible. These guidelines are less stringent and less specific than those that were recommended by the SCAR specialist group on birds, and represent a compromise to accommodate operational needs. While the adoption of clear and consistent guidelines for the operation of aircraft in Antarctica is welcome in that this provides practical advice that is likely to reduce incidences of close aircraft/bird encounters, there remains insufficient knowledge of the interactions between aircraft and birds in Antarctica, and the consequent impacts on individual birds and on bird populations. It is important, therefore, that the guidelines adopted are considered interim, and should be kept under scrutiny with revisions made as new and improved research results appear.     

The abundance of heterocystous blue-green algae in rice soils and inocula used for application in rice fields

Summary Algal populations were quantified (as colony-forming units [CFU] per square centimetre) in 102 samples of rice soils from the Philippines, India, Malaysia and Portugal, and in 22 samples of soil-based inocula from four countries. Heterocystous blue-green algae (BGA) were present in all samples. Nostoc was the dominant genus in most samples, followed by Anabaena and Calothrix. In soils, heterocystous BGA occurred at densities ranging from 1.0 × 10² to 8.0 × 10⁶ CFU/cm² (median 6.4 × 10⁴) and comprised, on average, 9% of the total CFU of algae. Their abundance was positively correlated with the pH and the available P content of the soils. In soil-based inocula, heterocystous BGA occurred at densities ranging from 4.6 × 10⁴ to 2.8 × 10⁷ CFU/g dw (dry weight), comprising only a moderate fraction (average 13%) of the total algae. In most soils, the density of indigenous N₂-fixing BGA was usually higher than that attained by applying recommended rates of soil-based inoculum. Whereas research on the practical utilization of BGA has been mostly directed towards inoculation with foreign strains, our results suggest that attention should also be given to agricultural practices that enhance the growth of indigenous strains already adapted to local environmental conditions.Maître de Recherches ORSTOM (France), Visiting Scientist at IRRI