Comparison of volatile constituents of Persicaria odorata(Lour.) Soják (Polygonum odoratum Lour.) and Persicaria hydropiper L. Spach (Polygonum hydropiper L.)

Polygonum odoratum Lour. has been reclassified as Persicaria odorata (Lour.) Soják [Wilson, K. L. Polygonum sensu lato (Polygonaceae) in Australia. Telopea 1988, 3, 177-182]; other synonyms currently used are Vietnamese mint or Vietnamese coriander and, in Malaysia, Daun Laksa or Laksa plant. The aerial parts of Laksa plant are highly aromatic, and they contain many organic compounds such as (Z)-3-hexenal, (Z)-3-hexenol, decanal, undecanal, and dodecanal that are typical for green, citrus, orange peel, and coriander odors. In addition to these aldehydes, 3-sulfanyl-hexanal and 3-sulfanyl-hexan-1-ol were discovered for the first time in this herb. The fresh leaves are pungent when they are chewed, although the active compound has never been identified. The pungency of Persicaria hydropiper (L.) Spach (formerly Polygonum hydropiper L., synonym water pepper) is produced by polygodial, a 1,4-dialdehyde derived from drimane terpenoids. We also identified polygodial as the active pungent compound in P. odorata (Lour.) Soják.     

Different Forms of Potassium and Their Contributions toward Potassium Uptake under Long-Term Maize (Zea mays L.)–Wheat (Triticum aestivum L.)–Cowpea (Vigna unguiculata L.) Rotation on an Inceptisol

In a long-term fertilizer experiment at the Indian Agricultural Research Institute, New Delhi, with maize, wheat, and cowpea, various forms of potassium (K) and their contribution toward K uptake were found to be affected by fertilizer use and intensive cropping. The treatments included for the study were a control, 100% nitrogen (N), 100% N–phosphorus (P), 50% NPK, 100% NPK, 100% NPK + farmyard manure (FYM at 15 t ha^?1 to maize only), and 150% NPK. The concentration of nonexchangeable K was greatest, followed by exchangeable K and water-soluble K. The study revealed no significant change in water-soluble K concentration in surface soil compared to N, NP, and control, indicating existence of an equilibrium between different K forms. Application of 100% NPK significantly increased water-soluble K concentration in surface soil compared to N, NP, and control treatments after maize, wheat, and cowpea. Application of NPK + FYM and 150% NPK resulted in greater quantities of all the K forms as compared to other treatments. Among the three forms, water-soluble K contributed predominantly to K uptake by maize and wheat; however, nonexchangeable K contributed significantly to K uptake by cowpea.     

Bemerkungen überRicciocarpus natans (L.) Corda

Zusammenfassung Untersuchungen an fruchtendem Material des LebermoosesRicciocarpus natans aus China ergaben, daß in einigen nicht unwesentlichen Punkten die in der Literatur zu findenden Angaben über die Art zumindest nicht generelle Gültigkeit haben.Ergebnisse der Chinesisch-deutschen Biologischen Sammelreise durch N und NO China 1956, Nr. 9.     

Translocation of 13C-labeled leaf or root litter carbon of beech (Fagus sylvatica L.) and ash (Fraxinus excelsior L.) during decomposition – A laboratory incubation experiment

The aim was to quantify medium term litter type and litter mixture effects on the translocation and transformation dynamics of root and leaf litter C during decomposition. Partitioning of ¹³C-labeled root or leaf litter C (beech – Fagus sylvatica L., ash – Fraxinus excelsior L.) to CO₂, water-extractable organic C (WEOC), microbial biomass C (C_MB) and light (LF) and heavy soil fraction (HF) was determined in a laboratory decomposition experiment of 206 days. The proportions of C mineralized from ash leaf (34%) and root litter (29%) were higher than those from beech leaf (24%) and root litter (23%). In mixture with beech, the mineralization of ash leaf litter was enhanced. Mineralization was positively correlated with litter-derived WEOC until day 29. Water-extractable organic C declined with time, until <0.1% of litter C remained in this fraction. Litter-C recovery in C_MB was higher for ash (0.7–1.0%) than for beech (0.2–0.4%). The litter C recovery in HF (4–12%) was positively correlated with that in WEOC (days 9 and 29) and C_MB, but did not differ between treatments. Ash leaf litter mineralization showed different behavior in mixed treatments from pure treatments. Thus, the ability to transfer results from pure to mixed treatments is limited. The litter differed in chemical composition and in mineralization dynamics, but differences in partitioning to HF, WEOC and MB were finally of minor importance.     

Behaviour of Potassium and Trace Elements in Rhizosphere of Flue-Cured Tobacco (Nicotiana Tabacum L.)

The study on the behaviour of potassium,phosphate and trace elements,Fe,Mn,Cu and Zn,in the rhizosphere of different varieties of flue-cured tobacco (Nicotiana tabacum L.)with high and low potassium application rate with rhizobag technique showed that soil available K,soil available P,and slow available K was in depletion status,whereas DTPA extractable Fe,Mn,Zn and Cu accumulated obviously in rhizosphere.The depletion and accumulation rates of mineral nutrients differed in degree with K application rate,soil type,and tobacco variety.The content of available K in both rhizosphere and bulk soil and K concentration in tobacco leaf increased significantly,and the available P in rhizosphere dropped with more K application.The DTPA-Fe content of red soil much lower in pH was higher than that of calcareous soil in bulk soil.But the DTPA-Fe content of calcareous soil was much higher than that of red soil in rhizosphere,which was considered perhaps to be mainly related to releasing of Fe phytosiderophore.Nitrate coule increase depletion of a vailable K in rhizosphere and also soil pH in comparision with ammonium.     

Effect of different safflower (Carthamus tinctorius L.)–bean (Phaseolus vulgaris L.) intercropping patterns on growth and yield under weedy and weed-free conditions

Two field experiments were carried out over two consecutive years (2010–2011) in the research field of the College of Agriculture, Shiraz University, Fars Province, southern Iran. The study was a factorial experiment based on a randomized complete block design with three replications: the first factor was the ratio of safflower (Carthamus tinctorius L. Pi cv.) to bean (Phaseolus vulgaris L. Saiad cv.) at five levels (safflower and bean sole cropping, and intercropping of safflower and bean at ratios of 1:3, 2:2 and 3:1); and the second factor was weed management at two levels: weed-free (complete weed control) and weedy (no weed control). The results showed that an intercropping system was the most appropriate method for decreasing the adverse effect of weeds on the performance of both crops. Intercropping was more suitable for weedy than weed-free conditions. According to the land equivalent ratio (LER) value, if the main crop was bean, the best intercropping treatment was one row of safflower and six rows of bean (S₁B₃) under both weedy and weed-free conditions. By contrast, if the main crop was safflower, the best treatment under weedy conditions was S₁B₃, whereas under weed-free conditions the best treatment was two rows of safflower and four rows of bean (S₂B₂). Overall, S₁B₃ can be introduced as the best intercropping method.     

Analysis of volatile compounds of taperebá (Spondias mombin L.) and cajá (Spondias mombin L.) by simultaneous distillation and extraction (SDE) and solid phase microextraction (SPME)

Volatile compounds of cajá and taperebá fruits, both classified as Spondias mombin, but from different geographic origins, were extracted (and analyzed) using solid phase microextraction (SPME) and simultaneous distillation and extraction (SDE). Forty-eight compounds were identified in taperebá and 47 in cajá by SPME using a DVB/CAR/PMDS fiber. (E)-Caryophyllene (18.7%), ethyl butyrate (10.0%), and ethyl hexanoate (7.0%) were the most abundant components in taperebá volatiles extracted by SPME, whereas myrcene (41.1%) and beta-phellandrene (8.5%) were the major compounds in cajá. In the taperebá SDE extract, 46 substances were identified, and (Z)-caryophyllene (13.2%) and limonene (9.5%) were predominant. From the 42 substances found in the SDE extract of cajá, the major components were myrcene (38.0%) and p-cymene (6.2%). The two fruits showed similar chromatograms upon the use of SDE and SPME. These methods made it possible to determine 30 identical components in both fruits by using SDE and 32 by using SPME.     

Essential Oil Composition of Norwegian Peppermint (Mentha × piperita L.) and Sachalinmint [Mentha sachalinensis (Briq.) Kudô]

Production of varieties of the medicinal plants Mentha     

Agro-morphological variation in “Jhinuwa” rice landraces (Oryza sativa L.) of Nepal

Jhinuwa is an aromatic rice (Oryza sativa L.) landrace from the Pokhara Valley of Nepal. A total of 210 accessions comprising seven types of Jhinuwa rice landraces were randomly collected from the rice fields to evaluate inter- and intra-population variability based on agro-morphological traits. The experiment was conducted in factorial randomized complete block design with three replicates in 2005. The first six principal components (PCs) accounted for 76.6 % variation for agro-morphological traits. Major traits that accounted for the variation by six PCs includes days to heading, days to maturity, total grain panicle^?1, fertile grain panicle^?1, culm length, panicle length, milling recovery, head rice recovery, aroma, 1,000 grain weight, sterile grain panicle^?1, grain sterility %, and leaf characteristics. Both principal coordinate analysis and cluster analyses revealed four phenotypic groups, two of which represent Bayarni, Jhinuwa, and Biramphul while the other two account for Tunde and Pakho Tunde. Tunde, Pakho Tunde, Kalo Bayarni, and Seto Bayarni showed higher intra- as well as inter-population variation compared to other populations. The phenotypic and genotypic coefficients of variation, broad sense heritability (h ²B) and genetic advance (GA) as a percent of the mean assessed for 210 accessions revealed high h ²B and GA estimates for leaf width, leaf length breadth ratio, ligule length, sterile grain panicle^?1, grain sterility % and 1,000 grain weight. The current study demonstrates that improvement in Jhinuwa rice landrace is possible by selecting superior accessions from existing natural populations while selection should be focussed to market traits with higher h ²B and GA estimates.     

Fatty acid composition of lipids in sea buckthorn (Hippophaë rhamnoides L.) berries of different origins

The oil content and fatty acid composition of berries from two subspecies of sea buckthorn (Hippopha? rhamnoides L.) were investigated. The berries of subsp. rhamnoides contained a higher proportion of oil in seeds (11.3% vs 7.3%, p < 0.01), berries (3.5% vs 2.1%, p < 0.001), and seedless parts (2.8% vs 1.7%, p < 0.01) than the berries of subsp. sinensis. Linoleic (18:2n-6) and alpha-linolenic acids (18:3n-3) comprised about 70% of seed oil fatty acids. Palmitoleic acid (16:1n-7), practically absent in the seed oil, comprised 12.1--39.0% of oil in pulp/peel and 8.9--31.0% of that in the whole berries. More linoleic acid (40.9% vs 39.1%) and less alpha-linolenic acid (26.6% vs 30.6%) was found in the seed oil of subsp. sinensis than in the seed oil of subsp. rhamnoides (p < 0.05). The proportion of palmitoleic acid was higher in the oil of berries of subsp. rhamnoides than the berries of subsp. sinensis (26.0% vs 21.5%, 0.05 < p < 0.1), but was vice versa with alpha-linolenic acid (8.8% vs 11.2%, 0.05 < p < 0.1). The proportions of alpha-linolenic acid correlated inversely with oleic and linoleic acids in the seed oil. In the oil of whole berries, the proportion of palmitoleic acid correlated negatively with the proportions of linoleic and alpha-linolenic acids.     

Influence of Mn and Fe Counteraction on Rice (Oryza sativa L.)Oranging Physiological Disease

Mn and Fe are two important micronutrients of paddy soils derived from red earths.Rice(Oryza sativa L.)oranging physiological diease in newly reclaimed red rarths is related to Fe toxicity.There have been considerable studies on Mn and Fe counteraction,but influence of Mn and Fe counteration on rice oranging physiological disease still remains unknown.This paper is to study,using two soils from USA and China respectively,the relationship between Nn and Fe counteraction and the physiological disease.Analysis for water soluble and extractable Fe and Mn showed that Mn/Fe ratios of the red earths were lower than those of the paddy soils.Fertilizing with Mn raised Mn/Fe and reduced oranging leaves,improved growth and increased yields.Analysis with electron probe showed that Mn treatment had less Fe deposit in root epidermis and more Ca and Si in roots.The results indicated that fertilizing with Mn could correct Fe toxicity.How to apply Mn and Fe counteraction in practice is worth further studying.     

Changes in triacylglycerol composition during ripening of sea buckthorn (Hippophaë rhamnoides L.) seeds

Changes in the quantitative composition of triacylglycerols (TAGs) in maturing sea buckthorn (Hippopha? rhamnoides L.) seeds were determined by lipase hydrolysis. As a whole, the rate of synthesis of separate TAG classes increased in proportion to both their unsaturation and relative content (weight percent) in total TAGs. Up to the 80th day of maturation, the formation of triunsaturated TAGs was predominant. Subsequently, at the terminal stage of seed ripening, the absolute content (in nanomoles per seed) of a major group of these TAGs containing linolenic and linoleic acyls decreased by approximately 7%, and the increase in the total TAG content was mainly due to the synthesis of TAG molecules including stearic and palmitic acyls in the rac-1,3 positions, as well as those containing oleate in the sn-2 position. At each maturation stage, the composition of the TAGs formed was controlled both by the composition of fatty acids available for TAG synthesis and by the rate of incorporation of a particular fatty acid into the sn-2 position of the TAGs.     

Secoisolariciresinol and matairesinol of sea buckthorn (Hippophaë rhamnoides L.) berries of different subspecies and harvesting times

Sea buckthorn (Hippopha? rhamnoides) seeds, berries, and berry fractions are often used as sources of bioactive ingredients for health products. The aim of the present study was to analyze lignans in these fractions of sea buckthorn. Secoisolariciresinol and matairesinol in seeds, fruit pulp/peel, and whole berries of sea buckthorn of three subspecies were analyzed by isotope dilution gas chromatography-mass spectrometry. The total content of the two lignans secoisolariciresinol and matairesinol varied widely from 8 to 139 microg/100 g in fresh berries and from 51 to 319 microg/100 g in dry berries. The content of secoisolariciresinol varied in the range of 34-313 microg/100 g of dry mass in the fruit pulp/peel and 93-355 microg/100 g in dry seeds. The content of matairesinol fell within the range of 3-25 microg/100 g in dry pulp/peel and 1-13 microg/kg in dry seeds. Wild H. rhamnoides ssp. sinensis contained a significantly higher total level of secoisolariciresinol and matairesinol in dry seeds, dry berries, and fresh berries compared with wild ssp. rhamnoides (253 vs 135 microg/100 g, P < 0.01, in seeds; 224 vs 153 microg/100 g, P < 0.05, in dry berries; 71 vs 29 g/100 g, P < 0.01, in fresh berries) and the cultivar of ssp. mongolica (253 vs 112 microg/100 g in seeds, 71 vs 9 microg/100 g in fresh berries). Harvesting dates had a significant influence on the content of the two lignans in seeds, fruit pulp/peel, and whole berries. This is the first report of lignans in sea buckthorn.     

Effect of nitrogen– boron interaction on plant growth and tissue nutrient concentration of canola (Brassica napus L.)

Pot experiments were conducted in the greenhouse on a calcareous soil to study the effect of nitrogen on the alleviation of boron toxicity in canola (Brassica napus L.). The treatments consisted of factorial combination of six levels of B (0, 2.5, 5, 10, 20, and 40 mg kg^?1 as boric acid), and four levels of nitrogen (N) (0, 75, 150 and 300 mg kg^?1 as urea) in a completely randomized design with three replicates. Boron (B) application significantly reduced the yield, whereas N addition alleviated the growth suppression effects caused by B supplements. Boron concentration increased with addition B. However, boron concentration in shoot declined with increasing N levels. Increasing N and B rates increased N concentration. Soil application of B increased proline concentration. However, the supply of N decreased it. Generally, application of B decreased potassium (K):B and calcium (Ca):B ratios, chlorophyll concentration, while N application increased them. It is concluded that N fertilization can be used effectively in controlling B toxicity in canola grown in B-affected soils.     

Leakage of a flavin‐like compound from lettuce (Lactuca sativa L.) under osmotic stress

Abstract

An unusual response vas noted in the salinized nutrient solutions of lettuce (Lactuca sativa L.) in that they became clear yellow in color. This condition also developed in experiments using polyethylene glycol in place of salt, suggesting that the response vas due to the osmotic stress imposed upon the roots. Photometric and fluorometric determinations indicated that the color could be attributed to a flavin‐like compound or compounds released by the roots.     

Effect of Organic Manure and Crop Residue Based Long-Term Nutrient Management Systems on Soil Quality Changes under Sole Finger Millet (Eleusine coracana (L.) Gaertn.) and Groundnut (Arachis hypogaea L.) – Finger Millet Rotation in Rainfed Alfisol

A long-term experiment was conducted to evaluate the effect of integrated use of organic and inorganic sources of nutrients on soil quality and its relation to finger millet yield under two predominant crop rotations viz., groundnut–finger millet and finger millet monocropping in hot moist semiarid rainfed Alfisol soils in South India. Two experiments were laid out separately for each cropping system in a randomized complete block design with five treatments individually with FYM and maize residue-based combinations viz., Control (T1), FYM @ 10t ha ^?1 or Maize residue (MR) @ 5t ha ^?1 (T2), farm yard manure (FYM) @ 10t ha ^?1 or Maize residue (MR) @ 5t ha ^?1 + 50% RDF (Recommended Fertiliser Dose) N, P₂O₅ &; K₂O (T3), FYM @ 10t ha ^?1 or Maize residue (MR) @ 5t ha ^?1 + 100% RDF N,P₂O₅ &; K₂O (T4), Recommended N, P₂O₅ &; K₂O (T5). Thus, four sets of nutrient management systems were evaluated. The results showed that farm yard manure or maize residue application in combination with recommended dose of fertilizer significantly improved the soil physical, chemical, and biological properties compared to control and application of inorganic fertilizers alone. Based on evaluation of 19 soil quality parameters under each of the four nutrient management systems, the common key soil quality indicators emerged out were: organic C (OC), available nitrogen (N), available sulfur (S), and mean weight diameter (MWD) of soil aggregates. A significant correlation between the finger millet yield and the relative soil quality indices (RSQI) indicates the importance of soil quality in these semiarid Alfisol soils. The results and the methodology adopted in the present study could be of importance in improving the soil quality not only for the region of the study, but also in other identical soils and cropping systems across the world.     

Transfer of rubidium‐86 (potassium) from deeply‐rooted alfalfa (Medicago sativa L.) to associated,shallow‐rooted maize (Zea mays L.) or grain sorghum (Sorghum vulgare pers.).

Circumstantial evidence exists for non‐N‐mineral element transfer in legume‐grass associations. Three experiments were conducted in an effort to directly demonstrate transfer of a non‐N‐mineral element in alfalfa (Medicago sativa L.)‐maize (Zea mays L.) and alfalfa‐grain sorghum (Sorghum vulgare Pers.) associations in two rooting media. Associations were established in double‐tube apparatus so that a single alfalfa plant was rooted in media of top‐ and bottom‐tubes, while an associated grass plant was rooted exclusively in the top‐tube (Intact treatment). Severed treatments (the control) were identical to the Intact treatments except the alfalfa roots in an air gap between the top‐and bottom‐tubes were excised.

⁸⁶Rb was dispensed onto the medium of bottom tubes with movement of the radioisotope determined by analyzing the legume and grass tissues over time. ⁸⁶Rb was detected in: i) soil‐grown maize associated with alfalfa within a 40‐day treatment period; ii) sand‐grown maize associated with alfalfa within 20 days after treatment and iii) sand‐grown sorghum associated with alfalfa within 10 days. Detection of ⁸⁶Rb in grass plants associated with alfalfa demonstrated that transfer of this potassium analog can occur via the root systems of legume‐grass associations.     

Predicting Post-Harvest Soil Test Values in Hybrid Rice (Oryza Sativa L.) – Wheat (Triticum Aesitvum L.) Cropping Sequence Using a Multivariate Analysis Technique

ABSTRACT

Knowledge of the initial soil fertility status is very crucial to make the soil test-based fertilizer recommendations and therefore it is necessary to develop alternative techniques to predict the post-harvest soil test values than analyzing the soils after every crop. The study was done to develop multiple linear regression (MLR) models to predict soil available nitrogen, phosphorus and sulfur in the hybrid rice-wheat cropping sequence. The post-harvest soil test values were considered as the dependent variable and initial soil nutrients applied nutrient through fertilizer and farmyard manure and grain yield as independent variables. In general, the accuracy of prediction for the calibration and validation models using the single year and two-year data model was significant and had a coefficient of determination was ≥0.75. Although the performance of MLR model to predict post-harvest soil N, P and S after the individual crop was better than that after whole rice-wheat cropping sequence, predictions of the post-rice-wheat sequence of soil N, P and S also had acceptable levels of accuracy. Thus, the concept of the using the MLR-based models to predict the post-harvest soil test values could be used in hybrid rice-wheat cropping sequence to make the soil test-based fertilizer recommendations to the individual crops or whole cropping sequence.     

Broad antifungal activity of β-isoxazolinonyl-alanine,a non-protein amino acid from roots of pea (Pisum sativum L.) seedlings

Summary -(Isoxazolin-5-on-2-yl)-alanine (IA), a heterocyclic non-protein amino acid from root extracts and root exudates of pea seedlings, acts as a potent growth inhibitor of several eukaryotic organisms, including yeasts, phytopathogenic fungi, unicellular green algae, and higher plants. The antibiotic effect on baker's yeast was reversed by l-methionine, l-cysteine, and l-homocysteine. Phytopathogenic fungi such as Botrytis cinerea, Pythium ultimum, and Rhizoctonia solani grown on agar containing IA were inhibited in the growth of mycelia or in the production of sclerotia. In contrast, no significant inhibition of either Gram-positive or Gram-negative bacteria was observed. Rhizobium leguminosarum, the compatible microsymbiont of Pisum spp., and Rhizobium meliloti were able to tolerate up to 2.9 mM IA (500 ppm) without any effect on the growth rate. Bradyrhizobium japonicum even gave a positive chemotactic response to IA. The ecological significance of IA as a preformed plant protectant during the seedling stage of Pisum spp. and other IA-containing legumes is discussed.     

Can the Activities of Acetylcholinesterase and Glutathione S-Transferases of Crangon crangon (L.) be Used as Biomarkers of Fuel Oil Exposure?

This work aimed to determine the sensitivity of acetylcholinesterase (AChE) and glutathione S-transferases (GST) activities of Crangon crangon (L.) to the water-accommodated fraction (WAF) of the fuel oil spilled by the oil tanker Prestige, in order to assess their usefulness as markers for this kind of pollution. Laboratory exposure of shrimps to WAF of weathered Prestige fuel oil showed no significant interference with AChE activity. Significant induction of GST activity was observed, potentially as a result of high concentrations of polycyclic aromatic hydrocarbons. However, these results were only obtained with the higher WAF concentrations tested. It was concluded that AChE and GST activities of C. crangon were not useful biomarkers for short-term exposure to the WAF of fuel oil spilled by the tanker Prestige and carried to the beaches of Galicia (NW Spain).