化感小麦种质资源的筛选与评价

以国内外90份不同小麦为材料,采用土壤-琼脂三明治法和田间杂草调查法,对不同小麦种质资源进行化感潜力评价,筛选出具有较强化感潜力的小麦品种。土壤-琼脂三明治法测试结果表明,不同小麦品种的化感潜力存在明显差异,对莴苣根长抑制率IR在10．1％和69．1％之间;筛选出“115／青海麦”、“92L89”、“百泉3199”、“81-214”和“92H31”5个强化感潜力的小麦品种,其对莴苣根长抑制率依次为69．1％、68．5％、68．7％、69．1％和65．2％,筛选出“抗10103（80）”和“A246”2个弱化感潜力的小麦品种,抑制率IR依次为10．1％和12．2％;田间杂草调查评价和土壤-琼脂三明治法表现出相似趋势,检测结果也得到了琼脂迟播共培法的佐证,表明采用土壤-琼脂三明治法筛选化感小麦种质资源是可行的。这为小麦化感作用研究提供了重要的种质资源,也为化感小麦种质资源的筛选提供方法借鉴。     

番木瓜酸性转化酶基因家族预测及生物信息学初步分析

酸性转化酶参与了番木瓜果实中蔗糖的韧皮部卸载,调控果实中糖类的积累。本文利用番木瓜基因组数据库信息,发现了番木瓜基因组中含有6个酸性转化酶基因,分别为细胞壁转化酶基因（CpCWINV1-3）和液泡转化酶基因（CpVINV1-3）。这些基因由3-7个外显子组成,其中CpCWINV1、CpVINV1和CpVINV2的N端均具有一个跨膜区域。蛋白质3D结构预测分析表明,CpCWINV1、CpVINV1和CpVINV2具有典型的N端β-螺旋桨模块（β-propeller module）和C端β-三明治模块（β-sandwich module）,而CpCWINV3和CpVINV3的桨叶I上,均没有保守区NDPNG/A;CpCWINV2和CpVINV3均缺失了β-三明治模块。推测番木瓜细胞壁转化酶CpCWINV1、液泡转化酶CpVINV1和CpVINV2能正确履行催化蔗糖分解的功能。本研究有助于我们进一步揭示酸性转化酶在番木瓜果实发育及糖类积累过程中的作用。     

福建省露地及设施蔬菜烟粉虱越冬种群密度及耐寒性研究

2017年1月9—12日和2月21—24日,在南平建瓯、三明将乐、三明三元、福州闽侯、龙岩新罗以及漳州龙海等6个地区,调查了大棚和露地烟粉虱越冬寄主及种群密度,以期明确福建省露地及设施蔬菜烟粉虱越冬规律,为烟粉虱的综合治理提供理论依据。共记录烟粉虱越冬寄主6科16种,主要包括栽培蔬菜花椰菜、番茄、茄子以及阔叶类杂草龙葵、小飞蓬、辣子草和胜红蓟。在露地花椰菜上,烟粉虱越冬虫口密度1月份显著高于2月份;花椰菜采后残株上的越冬虫口密度显著高于莲座期和花球形成期。在温室大棚中,休耕大棚中越冬虫口密度显著高于生产大棚。无论是蔬菜还是杂草,温室大棚中的越冬虫口密度显著高于露地。烟粉虱各虫态均可在各地温室大棚和漳州露地越冬,而在南平和三明等温度较低的地区以卵和伪蛹在露地越冬。在蔬菜上发现越冬烟粉虱有MEAM1隐种和MED隐种,温室大棚中两隐种数量分别为57只和111只,温室大棚可能有助于MED隐种定殖。室内耐寒性测定证实卵和高龄若虫比低龄若虫以及成虫更耐寒,烟粉虱在花椰菜、龙葵和辣子草上的世代存活率分别为83.7%、79.3%和81.6%,世代发育历期分别为18.9 d、16.7 d和18.8 d,龙葵和辣子草是烟粉虱适宜的越冬杂草寄主。综上,烟粉虱可在福建省露地越冬,温室大棚加重了烟粉虱的发生为害。     

基于资产负债法的福建省区域可持续发展能力研究

以福建省9个地级市为地理单元样本,从人口、经济、资源和环境4个方面选取涉及区域可持续发展能力的相关指标,采用资产负债方法评估了这些地理单元在这4个方面的可持续发展状况及总体可持续发展状况。结果表明,这些地理单元的总体可持续发展能力强弱的排序依次为厦门、福州、泉州、三明、龙岩、漳州、莆田、南平、宁德。其中前4名的净资产得分均大于0,处于可持续的状态;而后5名的净资产得分均小于0,处于不可持续的状态。以此为基础,进一步依据最优分割方法将这些地理单元划分为4种类型:可持续发展强型（厦门、福州、泉州）、可持续发展较强型（三明）、可持续发展较弱型（龙岩、漳州、莆田、南平）、可持续发展弱型（宁德）,并逐一分析了各个类型的可持续发展特征。     

山区单季晚稻枯叶早衰矫治技术研究

本文针对三明山区单季晚稻在生长发育中后期常发生的枯叶早衰这一生产问题 ,通过实地调查、土壤植株营养诊断和田间试验 ,认定是土壤缺素和植株营养失调所致 ,采取配方施肥和增施镁肥、石灰能有效地矫治水稻枯叶早衰 ,并取得显著的增产效果。     

三明市生态农业建设问题与对策研究

本在调查研究的基础上，论证并分析了三明市农业生态环境的现状及特点，并就生态农业建设的关键性问题进行讨论。根据三明自然社会条件及生态农业建设目标，提出了一套切实可行的对策。     

基于GIS的福建省烤烟气象灾害综合风险区划

为了探究福建复杂地形下的烤烟气象灾害风险,规避或减轻气象灾害可能造成的损失,利用福建省烟区28个县（区）的1972-2014年气象资料,2008-2013年烤烟产量、面积及其它社会经济资料,构建由致灾因子危险性、烤烟脆弱性和烟区防灾减灾能力组成的多灾种综合风险区划指标体系,采用层次分析法—熵权法量化计算各指标权重,利用加权综合法计算综合灾害风险指数,基于GIS技术开展烤烟气象灾害综合风险区划。结果表明：福建烟区气象灾害综合风险呈现由东南至西北逐级增加的趋势,轻度气象灾害综合风险区主要分布在龙岩市中东部、三明市东部和南平市东部的部分地带;中度灾害风险区主要分布在龙岩市西部,南平和三明两市介于东部和西部之间地带;重度以上风险区主要分布在南平和三明两市的西北部山区,其中1000m以上高海拔地区存在严重气象灾害风险。     

浅谈三明市东牙溪水源地生态环境存在的问题及防治对策

东牙溪是三明市区居民生产、生活重要的饮用水源地之一，近年来，由于人为因素和各种不合理的农事活动、生活污水等不经处理直接排入溪河，已危及到市区居民饮用水安全。本文通过对流域内水土流失现状、水质污染原因分析，提出相应的防治措施。     

韦伯-费希纳模型在湖泊(水库)预警评价中的应用

[目的]对福建省的莆田东圳水库、福清东张水库、三明东牙溪水库水环境质量及预警等级进行评价,为湖泊(水库)水环境预警评价提供一种过程简单,结果科学合理的评价方法。[方法]根据韦伯—费希纳模型(Weber-Fechner’s model)的基本原理,以表征水库水环境的溶解氧(DO),化学需氧量(CODMn),总磷(TP),NH_3-N,总氮(TN)等参数作为评价指标,利用水环境综合指数ki来评价水库水环境质量及预警等级。[结果]莆田东圳水库2010年第1,2季度以及2012年第3季度处于中重警。福清东张水库2013年第3季度为轻警,2012年第1季度处于中重警。三明东牙溪水库2010年第4季度、2011第3季度、2012年第3季度、2013第3,4季度为轻警。其余季度上述水库水质预警等级为中警。[结论]韦伯—费希纳模型能够定量描述湖泊(水库)水环境质量的感觉强度变化关系,客观反映湖泊(水库)水环境质量变化的影响;该模型具有计算过程简单,评价精度高,应用性强等优点,为湖泊(水库)水质预警等级综合评价提供了新方法,拓展了评价模型的应用范围。     

亚热带米槠天然林不同深度土壤胞外酶活性对隔离降雨的响应

随着全球变暖的加剧,降雨格局也在发生变化,土壤酶是森林生态系统化学过程的重要参与者,能够快速反映土壤环境的变化.因此研究亚热带土壤胞外酶活性对降水变化的响应,可有效评估气候变化对土壤有机质分解、转化的影响.以福建三明格氏栲自然保护区内的米槠天然林为研究对象,设置对照(CT)、隔离30％降雨(TE1)和隔离60％降雨(T...     

安阳市水保工程建管方式及存在的问题与建议

安阳市近10年来建成的水土保持工程的主要建设管理方式可分为3种,即:乡村组织治理,完成后分配给农户分散经营;乡村组织治理,由村成立专业队代表村集体管理;把小流域或荒山承包给个体或私人经济组织,由其自行治理和经营管护。3种建管方式中以后者为最优,其经济效益是分散经营管理的5倍、专业队经营管理的3.5倍。针对水保工程建设管理中存在的问题和适应农村税费改革等要求,提出了加快安阳市水保生态建设的建议。     

我国滑坡、崩塌的区域特征、成因分析及其防御

论述了我国滑坡、崩塌的区域分布特征,滑坡和崩塌的危害程度,滑坡和崩塌类型和成因分析,并且提出了灾害的防御措施,以期达到环境保护成为社会发展过程中的一个重要组成部分。     

生产建设项目水土保持“天地一体化”监管存在问题及建议

根据"天地一体化"技术在生产建设项目水土保持监管中的应用情况,对"天地一体化"技术在水土保持信息化应用中存在的防治责任范围图件不规范、合规性鉴定困难、未批先建等问题进行了归纳总结,并提出相应的建议,以期能为"天地一体化"水土保持监管工作逐步走向成熟提供参考,全面提升水土保持信息化建设水平,加快推进水土保持监管信息化进程。     

濂溪区水土保持区划与防治布局研究

水土保持区划可为区域水土保持规划与宏观管理、水土保持生态文明建设提供重要的参考。在《江西省水土保持区划及防治布局研究》确立的原则与方法的基础上,根据全国、全省和九江市水土保持区划划分方法,分析濂溪区各乡镇地形地貌、水土流失特点、水土流失防治及经济社会发展等的区域差异,将全区划分为城市人居环境维护区、平原农田防护水质维护区、丘陵保土生态维护区3个五级区,同时基于水土保持功能,提出了各分区的详细水土流失防治布局,为濂溪区及其他区域的水土保持规划与宏观决策,加快推进各地生态文明建设提供重要的参考和借鉴。     

Hydroxy-Al complexes of montmorillonite and vermiculite and identification of intergrades of montmorillonite-chlorite and vermiculite-chlorite in soils

Much evidence has indicated that the occurrence of montmorillonite and vermiculite containing Al-interlayers is very common in solis, particularly acid soils. Viewed in the light of soil clay minerology, it would be required to investigate the properties of their original minerals. For the latter purpose, the Al-interlayer has been removed prior to the Mg-glycerol,K-saturation and heating tests for identification of montmorillonite and vermiculite. Its removal has been accomplished in sevsral ways; KOH plus KCl (1), NH₄F, KCl plus HCl (2), Na-citrate (3),400C de hydroxylation-NaOH (4) dissolution. Although these methods are effective for the removal of Al-interlayers, they seem drastically to affect the minerals or are time-consuming. Inthe course of the study of hydroxy-Al interlaid complexes of expanding 2:1 layer lattice clay minerals, the authors have noticed the difference between the (001) spacings of hydroxy-Al complexes of montmorillonite and vermiculite; the former exhibited the 20 A basal spacing at room temperature against the 14 A spacing of the latter. This difference might be useful for differentiating montnmorillonite-chlorite intergrades in soils.     

硒、钴单施和配施对苜蓿生长及产量的效应研究

为探讨硒、钴在提高苜蓿生长影响,在河南省沿黄滩区,开展了硒、钴单施和配施下对紫花苜蓿生长以及产量和品质影响研究。结果表明,硒、钴单施和配合基施均能够显著提高苜蓿从现蕾到开花期的净光合能力,降低呼吸消耗,提高单位叶面积干物质的生产速率,有利于苜蓿花前青干草生产和果实发育;其中,以硒570 g/hm2、钴762g/hm2配施下效果显著,青干草增产8.06%,种子千粒重可提高12.85%。单施钴和硒钴配施还能促进0—20 cm耕层苜蓿根瘤菌的生长和积累,增强苜蓿的固氮能力。说明硒钴合理基施对苜蓿青干草和优质种子生产具有很好的应用效果。     

Separation and analysis of diastereomers and enantiomers of cypermethrin and cyfluthrin by gas chromatography

Synthetic pyrethroid (SP) insecticides are of environmental significance because of their high aquatic toxicity. Due to their chirality, SP compounds contain multiple diastereomers and enantiomers. However, due to great structural similarities and lack of isomer standards, gas chromatographic (GC) analysis of SP diastereomers or enantiomers is poorly developed. In this study, we used a HP-5 column to separate the diastereomers and a beta-cyclodextrin-based enantioselective column (BGB-172) to separate the enantiomers of cypermethrin (CP) and cyfluthrin (CF). Resolved peaks were identified by comparing chromatograms of isomer-enriched CP products. Diastereomers of both CP and CF were separated on the HP-5 column. On the BGB-172 column, enantiomers of all cis diastereomers were separated, while those of trans diastereomers were not separated. The elution order appears to be regulated by configuration, a finding which may allow peak identification in the absence of isomer standards. When coupled with electron capture detection, the developed methods had low detection limits and may be used for analysis of SP diastereomers and enantiomers in environmental samples.     

铁铝氧化物和高岭石对转化酶的吸附及活性的影响

Experiments were conducted to study the influences of synthetic bayerite, non-crystalline aluminum oxide (N-AlOH), goethite, non-crystalline iron oxide (N-FeOH) and kaolinite on the adsorption, activity, kinetics and thermal stability of invertase. Adsorption of invertase on iron, aluminum oxides fitted Langmuir equation. The amount of invertase held on the minerals followed the sequence kaolinite > goethite > N-AlOH > bayerite > N-FeOH. No correlation was found between enzyme adsorption and the specific surface area of minerals examined. The differences in the surface structure of minerals and the arrangement of enzymatic molecules on mineral surfaces led to the different capacities of minerals for enzyme adsorption. The adsorption of invertase on bayerite, N-AlOH, goethite, N-FeOH and kaolinite was differently affected by pH. The order for the activity of invertase adsorbed on minerals was N-FeOH > N-AlOH > bayerite > reak goethite > kaolinite. The inhibition effect of minerals on enzyme activity was kaolinite > crystalline oxides > non-crystalline oxides. The pH optimum of iron oxide- and aluminum oxide-invertase complexes was similar to that of free enzyme (pH 4.0), whereas the pH optimum of kaolinite-invertase complex was one pH unit higher than that of free enzyme. The affinity to substrate and the maximum reaction velocity as well as the thermal stability of combined invertase were lower than those of the free enzyme.     

河道堤防滩地的水土流失及其防治

平原地区河道堤防滩地的水土流失,直接淤积河床,影响行洪安全。堤防滩地的水土流失是自然因素和人为因素共同作用的结果,以新修堤防的水土流失最为严重,对其防治须实行工程措施、植物措施和人为预防相结合。     

Effects of residues of lime and phosphorus fertilizer on cadmium uptake and yield of potatoes and carrots

Potatoes (Solarium tuberosum L.) and carrots (Daucus carota L.) were grown in the field on Tasmanian ferrosols (humic eutrudox) which had been limed either 2, 3, or 5 years earlier, and where tuber cadmium (Cd) concentrations in potatoes grown a few months after the liming had shown no lime response. In the current crops lime decreased potato tuber Cd by about 30% and carrot root Cd by about 50%. We attributed the decrease to more even and deeper mixing of the lime with the soil by the harvest of the first potatoes. Phosphorus (P) fertilizer residues from the earlier potato crops did not significantly affect tuber or root Cd, but there was a positive effect at 1 site where some high Cd P fertilizer had earlier been used. Neither lime nor P fertilizer residues affected potato or carrot yields. Analysis at one site of potato tubers from the upper part of the soil ridges showed that they had slightly higher Cd concentrations than did deeper tubers near the fertilizer band, whether P fertilizer was in the band or not. This suggests that either the Cd in the fertilizer band was relatively unimportant as a Cd source for the current crop, or that Cd was redistributed within the plant during the season, or both. Liming may be a suitable medium to long‐term strategy for decreasing Cd uptake by root crops, but site to site and seasonal variation can still be great, and knowledge of other major influences is needed for assurance of produce quality. Our observations need to be extended to sites which gives rise to higher Cd concentrations in agricultural produce, and to other soil types. Potato common scab was severe in the limed plots at one site. However, this site had grown 3 potato crops in 5 years, which probably exacerbated the disease. Potato processors in Tasmania demand a minimum of 5 years between successive crops which should slow any build up of scab due to liming, but more work on possible interactions between lime and rotation length on scab incidence is needed before liming can be recommended as a Cd control measure for potatoes.