成都平原水旱轮作种植下土壤养分特性空间变异研究

肥料是作物的粮食,研究表明,施肥对粮食增产的贡献率可高达50%以上[1]。但是,我国长期存在的盲目的、非精准的不合理施肥,导致肥料当季利用率较低,特别是氮、磷肥。这既增加了种植成本,又造成水体富营养化[2,3]。土壤养分特性的空间变异研究对于实现田间土壤养分精准管理、变量施肥、提高肥料利用率等都具有非常重要的意义。国外对土壤养分特性空间变异的研究始于20世纪70年代,许多土壤学家将GIS和地统计学技术引入土壤学领域研究土壤属性的空间变异规律[4,5],并取得很好的结果。国内自90年代中期以来,许多研究者都对土壤属性特别是土壤养分的空间变异进行了研究[6~8],但主要侧重于对土壤物理性质和土壤盐     

基于GIS的优质小麦变量施肥信息系统研究

变量施肥量的确定在优质小麦生产中起着重要的作用，依据麦田土壤养分的空间变异性与小麦的生长特性因地制宜确定施肥量能为小麦优质生产提供施肥决策支持。该文是在施肥推荐中利用Kriging插值方法对研究区土壤养分空间变异性进行研究，解决以往变量施肥系统对土壤养分的供应量存在疑惑的问题；综合考虑土壤类型、障碍层次等土壤基础信息，将“氮肥后移”技术写入曲劳与斯坦福公式中进行施肥推荐，以实现小麦的优质生产。详细阐述了土壤养分空间变异分析和变量施肥推荐两个关键技术。     

奉新毛竹林土壤养分空间变异性研究

利用地统计学的半方差函数,定量研究了奉新县平衡施肥毛竹林的土壤养分空间变异特征。结果表明,毛竹林土壤养分存在半方差结构,多数为球状模型。奉新毛竹林土壤的变程为32～398.m,取样距离15～20.m,能满足毛竹林土壤空间变异评价的要求。N、P、K等养分的空间相关性小,人为经营、施肥等措施对土壤速效N、P、K的影响大于Ca、B、Fe、Mg等养分。奉新毛竹林土壤养分的分数维值排列顺序为:Cu>Mn>全P>Zn>P>N>Fe>K>Mg>S>OM>Ca>B>全K>全N。施肥时间长对于养分的空间分布均匀性有调和、促进作用。毛竹林土壤养分结构性变异大于随机变异,土壤内在属性(土壤矿物和地形)比人为因素(栽培管理水平和施肥措施)的影响大。     

蔬菜生产平衡施肥应用技术

平衡施肥技术是根据不同农作物不同时期的生理需求和不同土壤类型的养分含量,合理地供给作物生长发育必需的各种营养元素,使其能够均衡地满足植物生长的科学施肥技术。     

抑菌剂浇施与不均匀施肥对玉米马铃薯生长和产量的影响

农业生产中,肥料和抑菌性农药施用是两种重要的农业生产技术。在施肥过程中,点状和条状施肥是主要的施肥方式,易导致作物生长期内土壤养分以斑块状分布,因此根系趋肥性对农田中作物获取养分具有重要性。而在施用抑菌性农药时,药剂能够通过淋溶等过程进入土体中,对土壤生态环境和根-土过程产生直接或间接的影响。然而目前有关农药施用是否影响作物根系趋肥性,进而改变产量表现还不清楚。本研究选用旱地主要粮食作物玉米和马铃薯为研究对象,通过等量肥料下隔行施用的方式构建土壤养分斑块,在此基础上进行广谱性杀菌剂浇施土壤,研究抑菌性农药对作物利用异质性养分的影响。两年的大田试验数据表明,一定程度上,抑菌剂浇施和隔行施肥能够显著地影响作物的植株生物量、产量,根系生物量及分布,且对玉米生物量影响具显著交互效应,表现为隔行施肥对生物量的显著提高发生在抑菌剂浇施条件下,而抑菌剂对玉米生物量的提高则主要表现在隔行施肥条件下。同时,抑菌剂浇施能够提高作物的根系觅养精确度,其中在马铃薯上达到显著水平,表明抑菌剂浇施对作物适应土壤养分斑块具有一定的促进作用。当然,抑菌农药和养分斑块在影响作物生长过程中的显著性受作物类型和种植年份的影响,具有复杂性。因此,进一步针对不同作物、生态环境和栽培措施,探讨抑菌剂农药在作物适应养分斑块中的作用以及对作物根系趋肥的影响机制,对于了解农药施用对化肥利用的影响具有潜在的价值。     

天气气候条件与作物养分摄取特征的关系

<正> 天气气候条件不仅直接影响施肥的有效性,而且通过影响作物生长状况,间接地改变作物对营养元素的需求量及摄取能力.因此研究施肥理论必须将"土壤-作物-大气"看成一个系统,以联系的、动态的观点分析养分     

肥料养分真实利用率计算与施肥策略

目前人们提到的我国肥料利用率数据都是基于常规公式获得的当季肥料表观利用率的计算结果,并由此认定我国肥料利用率比较低。本文提出养分真实利用率的计算方法,养分真实利用率是作物吸收的养分占种植作物消耗的养分比例。养分来源既包括施用的各种肥料养分,也包括土壤贮存的养分,其公式为:作物吸收的养分量/(施肥量-土壤养分的盈亏量)。养分真实利用率也可采用100%减去养分的损失率来计算,因各种途径未被作物吸收利用并离开耕层土壤的养分才是损失,而贮存在耕层土壤中的养分则不能算作损失。我国耕地土壤肥力的维持和作物吸收的养分都主要来自于各种肥料,因而养分真实利用率的计算公式,基本等同于肥料养分真实利用率的计算公式。利用这一新的公式,我国的肥料养分真实利用率无论是当季还是长期利用率都将远远高于当前文献报道的数据。新的算法获得的肥料养分利用率直接与肥料养分的损失率相对应,将有利于揭示肥料对粮食生产的实际贡献率和损失率,并推动土壤肥力的培育目标及施肥策略与养分损失率相对应,促进施肥技术向减少肥料损失的方向发展,而不是片面地提高目标不太明确却广泛使用的肥料表观利用率。未来大田作物的施肥量并不需要高度依赖测土施肥,而应主要依赖于作物生长对养分的消耗量和养分损失率来确定。     

菜地土壤养分的空间变异特征

通过地统计学、空间分析方法及结合农户施肥调查,对菜地土壤养分的空间变异特征进行研究。结果表明,农户施肥水平差别较大,整体上施磷相对过多,施钾相对较少,土壤OM、NH4+-N、P、K、Ca、Mg和S的变异系数在28.4%～69.2%之间。土壤各养分的空间变异结构有较大区别,土壤OM及NH4+-N的变异具有强烈空间相关性,土壤P、K、Ca、Mg和S养分含量的变异为中等空间相关性。土壤养分的等值线图显示,OM在空间的分布与土壤质地渐变规律相关,NH4+-N呈现南北向的带状分布,P、K、Ca、Mg和S趋于小块状分布。总的来说,施肥等人为因素加剧了土壤矿质养分的空间变异。     

变量施肥对改善土壤养分空间差异性的综合评价

精准农业中一个非常重要的环节就是变量施肥,其核心思想是根据土壤中养分含量的多少来决定施肥量,以达到土壤养分平衡。目前通常对土壤养分情况评价的做法是分别考察每种土壤养分的变异情况,不能综合分析。因此,该文提出利用一种加权模糊聚类算法,综合评价经过变量施肥作业后土壤养分空间差异性的变化情况。通过对榆树市弓棚镇十三号村3号地未变量施肥、连续变量施肥2年和连续变量施肥5年的土壤养分进行综合分析比较,可知经过连续变量作业后土壤养分空间差异明显减小。     

长期施肥对黑土和暗棕壤土壤酶活性及土壤养分的影响

为阐明长期施肥对黑土和暗棕壤土壤酶活性的影响、土壤酶活性与土壤养分的关系,探讨土壤持续利用的培肥模式,采集了施用不同肥料28年后的黑土和暗棕壤,对黑土和暗棕壤土壤酶活性以及土壤养分因子进行了测定和分析。结果表明,与不施肥对照相比,各施肥处理均可显著增加黑土和暗棕壤有机质和氮磷钾养分含量,提高土壤脲酶、磷酸酶、转化酶、过氧化氢酶活性,其中以化肥配施有机肥效果最为明显。土壤酶活性与土壤养分之间有很好的相关性,土壤脲酶、磷酸酶、转化酶、过氧化氢酶活性与土壤有机质、全氮、全磷、碱解氮、速效磷含量呈显著相关,黑土对施肥比暗棕壤对施肥更敏感。对不同土壤长期化肥配施有机肥均可为作物生长创造良好的土壤环境。     

Diffusion of NH+4 and NO−3 mineralized from organic N in soil

The mineralization of native soil organic matter and the simultaneous diffusion of zero NH⁺₄ and NO^?₃ to a solution sink of zero N concentration was analysed experimentally and theoretically for a fine sandy loam soil. Experimentally, the NH₄ and NO₃ ions produced in an incubated unsaturated soil column were allowed to diffuse through a sintered glass plate into a stirred solution sink. The distribution of NH⁺₄ and NO^?₃ in the soil column was measured after various incubation times. The rate of ammonification was measured directly during incubation and the rate of nitrification modelled from nitrifier growth kinetics. A Freundlich equation was used to describe the equilibrium between soluble and exchangeable NH⁺₄ in the soil. Terms for the microbial transformation of N and the adsorption-desorption of NH⁺₄ were combined with diffusion equations which were solved numerically using finite difference methods. The model constructed was used to predict the NH⁺₄ and NO^?₃ con-centration distributions in the soil column, and good agreement was obtained between the experimental and predicted concentration profiles. The use of the model for predicting the diffusive flux of mineral N to the outer surfaces of soil peds, where it is vulnerable to leaching, was demonstrated.     

Chemical and colloidal stability of sols in the Al2O3-Fe2O3-SiO2-H2O system: their role in podzolization

Concentrations of dialysable silica in equilibrium with Al₂O₃-SiO₂-H₂O sols at pH 4.5–5.0 confirm the formation of a poorly ordered non-dialysable proto-imogolite species with an Al : Si ratio near 2, close to that of imogolite. Sols with Al : Si>2 give nearly constant levels of free silica in solution in the range 2–6 μg/cm³, indicating equilibrium between proto-imogolite and aluminium hydroxide species. These findings indicate that imogolite-like precipitates in acid soils will buffer silica in solution to within this range during leaching episodes. Imogolite is more stable than a previous estimate suggested, and a revised value for its free energy of formation is proposed: ΔG⁰_f(298.15) = -2929.7 kJ/mol. In Fe₂O₃-SiO₂-H₂O sols, the Fe : Si ratio of the non-dialysable species varies smoothly from 11 to 3 as free silica in solution ranges from 4 to 35 μg/cm³. Such sols are much less colloidally stable than hydroxyaluminium silicate sols, but mixed Al₂O₃—Fe₂O₃—SiO₂—H₂O sols are almost as stable as iron-free sols up to a Fe : Al ratio of 1.5. Thus migration of Al and Fe as mixed hydroxide sols can account for the almost constant ratio of Al to Fe with depth in oxalate extracts from Bs horizons of podzols.     

Evidence that fungi can oxidize NH4+ to NO3− in a grassland soil

The contribution of bacteria and fungi to NH₄⁺ and organic N (N_org) oxidation was determined in a grassland soil (pH 6.3) by using the general bacterial inhibitor streptomycin or the fungal inhibitor cycloheximide in a laboratory incubation study at 20°C. Each inhibitor was applied at a rate of 3 mg g^?1 oven‐dry soil. The size and enrichment of the mineral N pools from differentially (NH₄¹⁵NO₃ and ¹⁵NH₄NO₃) and doubly labelled (¹⁵NH₄¹⁵NO₃) NH₄NO₃ were measured at 3, 6, 12, 24, 48, 72, 96 and 120 hours after N addition. Labelled N was applied to each treatment, to supply NH₄⁺‐N and NO₃^?‐N at 3.15 μmol N g^?1 oven‐dry soil. The N treatments were enriched to 60 atom % excess in ¹⁵N and acetate was added at 100 μmol C g^?1 oven‐dry soil, to provide a readily available carbon source. The oxidation rates of NH₄⁺ and N_org were analysed separately for each inhibitor treatment with a ¹⁵N tracing model. In the absence of inhibitors, the rates of NH₄⁺ oxidation and organic N oxidation were 0.0045 μmol N g^?1 hour^?1 and 0.0023 μmol N g^?1 hour^?1, respectively. Streptomycin had no effect on nitrification but cycloheximide inhibited the oxidation of NH₄⁺ by 89% and the oxidation of organic N by more than 30%. The current study provides evidence to suggest that nitrification in grassland soil is carried out by fungi and that they can simultaneously oxidize NH₄⁺ and organic N.     

Comparison of volatile generation in serine/threonine/glutamine-ribose/glucose/fructose model systems

Thermal generation of volatiles in nine model reactions was studied and compared. Each of the model systems contained one amino acid and one monosaccharide. The amino acid was serine, threonine, or glutamine, and the monosaccharide was ribose, glucose, or fructose. More unsubstituted pyrazine was generated in serine-sugar systems than threonine-sugar systems. The formation of several furfuryl-substituted pyrazines and pyrroles was observed in some of the studied systems. Total pyrazines were generated more in glutamine-containing systems than in serine- and threonine-containing systems, and the reverse was true for generation of furfuryl-substituted compounds. Acetylpyrazine was generated in serine/threonine/glutamine-glucose and serine/glutamine-fructose systems.     

Abundance of Frankia from Gymnostoma spp. in the rhizosphere of Alphitonia neocaledonica, a non-nodulated Rhamnaceae endemicto New Caledonia

The capacity of different soils of New Caledonia to induce nodulation in Gymnostoma poissonianum was studied. The soils were sampled under five Gymnostoma species, Alphitonia neocaledonica (a non-nodulated endemic Rhamnaceae) and Pinus caribea (an introduced species) growing in various ecological conditions. Using G. poissonianum as trap-host, we observed a higher abundance of Frankia from Gymnostoma spp. in the rhizosphere of A. neocaledonica as compared with bare soils and P. caribea rhizosphere. The nodulating capacity of A. neocaledonica rhizosphere was almost similar to that of the five Gymnostoma species (symbiotic host) studied in the same stations. In comparison, bare soils or rhizosphere of P. caribea had poor nodulating capacities. We isolated fourteen Frankia strains from nodules of G. poissonianum after baiting with the rhizospheric soils of five Gymnostoma and A. neocaledonica. Using the PCR/RFLP method, we confirmed the similarity with those already described. Frankia was abundant in the rhizosphere of A. neocaledonica in all the sites studied. One explanation could be a positive tropism of Frankia towards species belonging to families having nodulated species, which is the case of A. neocaledonica endemic in New Caledonia. We can suppose that the non-nodulated plants belonging to these families can excrete some chemical substances able to attract Frankia and to induce its proliferation.     

Determination of kC and kNin situ for calibration of the chloroform fumigation-incubation method

¹⁴C-labelled glucose and ¹⁵N-labelled KNO₃ were added to soil and the microbial biomass during 42 days' incubation was estimated using the chloroform fumigation-incubation method (CFIM). By day 1, most of the glucose (1577 μgCg^?1 soil) was metabolized and 110 μg NO^?₃-Ng^?1 soil were immobilized. In situ values for the proportions of biomass C (k_C) and biomass N (k_N) mineralized during the 10 days after CHCl₃ fumigation were determined on the basis that the immobilized labelled C and N remaining in the soil at this time were present as living microbial cells and their associated metabolites. The tracer data indicated that biomass C could be calculated by applying a k_c value of 0.41 to the CO₂-C evolved from the fumigated sample without subtraction of an unfumigated “control”. Biomass N was estimated from the net NH₄^?-N accumulation during the fumigation-incubation. The problem of reimmobilization of NH⁺₄-N where organisms of wide C:N ratio occur was overcome by adjusting the value of k_N according to the ratio of CO₂-C evolved: net NH₄⁺-N accumulated during the fumigation-incubation (C_F:N_F).A C_F:N_F ratio of 6:1 resulted in a k_N of 0.30 whereas a ratio of 13:1 indicated a k_N of 0.20.     

TRANSFORMATION OF 15N-LABELLED AMMONIUM IN TWO SOILS DIFFERING IN NH+4-FIXING CAPACITY

Two soils differing in ammonium fixation capacity were incubated for 127 days with ¹⁵N-ammonium sulphate. In a gley soil with high NH⁺₄-fixing capacity caused by smectites with a charge up to 0.8 per formula unit, the major part of the added ammonium was first fixed by minerals and then released slowly during incubation. The proportion of labelled N in the nitrate fraction increased during the first weeks and then decreased permanently. In contrast, in a histosol with low NH⁺₄-fixing capacity, the exchangeable fraction contained most of the labelled NH⁺₄, this being highly available to microorganisms and therefore subject to nitrification. About 50% of the added ¹⁵NH₄ was lost from the histosol in 127 days, but only about 20 per cent was lost from the gley soil.     

Incorporation studies of NH+4 during incubation of organic residues by l5N-CPMAS-NMR-spectroscopy

This study focuses on the processes occurring during incorporation of inorganic nitrogen into humic substances. Therefore rye grass, wheat straw, beech saw dust, sulphonated lignin and organosolve lignin were incubated together with highly ¹⁵N-enriched ammonium sulphate in the laboratory for 600 days. Samples from the incubates were periodically analysed for weight loss, and carbon and nitrogen contents. The samples were also analysed by solid-state ¹³C- and ¹⁵N-CPMAS-NMR-spectroscopy to follow the turnover of the materials during incubation. Most of the detectable N-signals was assigned to amide - peptide structures. The remaining intensities could be ascribed to free and alkylated amino groups, and those on the low field side of the broad amide-peptide signal to indole, pyrrole and nucleotide derivatives. Abiotic reactions of ammonia with suitable precursors and the formation of pyridine, pyrazine or phenyloxazone derivatives were not observed. Signals from ammonia and nitrate occurred only at the end of the incubation.     

Cu/Pb/Zn/Cd在石英砂中的迁移实验及模拟

通过稳定流混合置换实验,研究了孔隙水流速和pH变化对Cu/Pb/Zn/Cd在石英砂中迁移行为的影响,获得了示踪剂Br-和Cu/Pb/Zn/Cd的穿透曲线(BTCs);并通过室内批量平衡实验获得吸附系数,进而计算出阻滞因子Rd。基于这些实验结果,借助CXTFIT2.1软件,用平衡CDE模型拟合了Br-的BTCs,得到了弥散系数D;在此基础上应用CDE非平衡模型拟合Cu/Pb/Zn/Cd在不同流速和pH条件下的BTCs,并预测了平行实验和不同埋深处Cu/Pb/Zn/Cd浓度的动态变化。结果表明,Cu/Pb/Zn/Cd的迁移能力随流速的增大而增强,而随pH的增大而降低;化学非平衡的两点模型能较好地模拟本文实验条件下Cu/Pb/Zn/Cd的迁移过程。