有机物料输入稻田提高土壤微生物碳氮及可溶性有机碳氮

土壤微生物量碳、氮和可溶性有机碳、氮是土壤碳、氮库中最活跃的组分,是反应土壤被干扰程度的重要灵敏性指标,通过设置相同有机碳施用量下不同有机物料处理的田间试验,研究了有机物料添加下土壤微生物量碳（soil microbial biomass carbon,MBC）、氮（soil microbial biomass nitrogen,MBN）和可溶性有机碳（dissolved organic carbon,DOC）、氮（dissolved organic nitrogen,DON）的变化特征及相互关系。结果表明化肥和生物碳、玉米秸秆、鲜牛粪或松针配施下土壤微生物量碳、氮和可溶性有机碳、氮显著大于不施肥处理（no fertilization,CK）和单施化肥处理,分别比不施肥处理和单施化肥平均高23.52%和12.66%（MBC）、42.68%和24.02%（MBN）、14.70%和9.99%（DOC）、22.32%和21.79%（DON）。化肥和有机物料配施处理中,化肥+鲜牛粪处理的微生物量碳、氮和可溶性有机碳、氮最高,比CK高26.20%（MBC）、49.54%（MBN）、19.29%（DOC）和32.81%（DON）,其次是化肥+生物碳或化肥+玉米秸秆处理,而化肥+松针处理最低。土壤可溶性有机碳质量分数（308.87 mg/kg）小于微生物量碳（474.71 mg/kg）,而可溶性有机氮质量分数（53.07 mg/kg）要大于微生物量氮（34.79 mg/kg）。与不施肥处理相比,化肥和有机物料配施显著降低MBC/MBN和DOC/DON,降低率分别为24.57%和7.71%。MBC和DOC、MBN和DON随着土壤有机碳（soil organic carbon,SOC）、全氮（total nitrogen,TN）的增加呈显著线性增加。MBC、MBN、DOC、DON、DOC+MBC和DON+MBN之间呈极显著正相关（P<0.01）。从相关程度看,DOC+MBC和DON+MBN较MBC、DOC、MBN、DON更能反映土壤中活性有机碳和氮库的变化,成为评价土壤肥力及质量的更有效指标。结果可为提高洱海流域农田土壤肥力,增强土壤固氮效果,减少土壤中氮素流失,保护洱海水质安全提供科学依据。     

北松辽平原沿纬度梯度农田土壤酶活性与微生物量分布研究

Soil enzymes activities and microbial biomass have an important influence on nutrient cycling. The spatial distribution of soil enzymes activities and microbial biomass were examined along a latitudinal gradient in farmlands of Songliao Plain, Northeast China to assess the impact of climatic changes along the latitudinal transect on nutrient cycling in agroecosystems. Top soils （0-20 cm depth） were sampled in fields at 7 locations from north （Hallun） to south （Dashiqiao） in the end of October 2005 after maize harvest. The contents of total C, N, and P, C/N, available N, and available P increased with the latitude. The activities of invertase and acid phosphatase, microbial biomass （MB） C and N, and MBC/MBN were significantly correlated with latitude （P 〈 0.05, r^2 = 0.198, 0.635, 0.558, 0.211 and 0.317, respectively）, that is, increasing with the latitude. Significant positive correlations （P 〈 0.05） were observed between invertase activity and the total N and available P, and between acid phosphatase activity and the total C, C/N, available N, total P and available P. The urease, acid phosphatase, and dehydrogenase activities were significantly correlated with the soil pH and electrical conductivity （EC） （P 〈 0.05）. MBC and MBN were positively correlated with the total C, C/N, and available P （P 〈 0.05）. The MBC/MBN ratio was positively correlated with the total C, total N, C/N, and available N （P 〈 0.05）. The spatial distribution of soil enzyme activities and microbial biomass resulted from the changes in soil properties such as soil organic matter, soil pH, and EC, partially owing to variations in temperature and rainfall along the latitudinal gradient.     

土壤微生物量碳氮作为土壤肥力指标的探讨

通过对不同肥力水平黑土、棕壤、黄棕壤、红壤土壤微生物量碳氮的比较,研究和探讨土壤微生物量碳氮与土壤肥力的关系。结果表明:有机肥或无机肥施入土壤均能增加土壤微生物量C、N的含量,且有机肥与无机肥配合有显著的正交互作用。土壤微生物量C、N从北到南的地带性变化规律不强。BC/BN及TC/TN从北到南逐渐减小,BC/TC及BN/TN从北到南逐渐增加。相关分析结果表明:土壤微生物量C、N与全C、全N均呈极显著相关,可以作为指示土壤肥力的重要指标。     

长期稻草还田对土壤球囊霉素和土壤C、N的影响

A long-term experiment was conducted to investigate how long-term fertilization and rice straw incorporation into soil affect soil glomalin, C and N. The combined application of chemical fertilizer and straw resulted in a significant increase in both soil easily extractable glomalin （EEG） and total glomalin （TG） concentrations, as compared with application of only chemical fertilizer or no fertilizer application. The EEG and TG concentrations of the NPKS （nitrogen, phosphorus, and potassium fertilizer application ＋ rice straw return） plot were 4.68% and 5.67% higher than those of the CK （unfertilized control） plot, and 9.87% and 6.23% higher than those of the NPK （nitrogen, phosphorus, and potassium fertilizer applied annually） plot, respectively. Application of only chemical fertilizer did not cause a statistically significant change of soil glomalin compared with no fertilizer application. The changes of soil organic C （SOC） and total N （TN） contents demonstrated a similar trend to soil glomalin in these plots. The SOC and TN contents of NPKS plot were 15.01% and 9.18% higher than those of the CK plot, and 8.85% and 14.76% higher than those of the NPK plot, respectively. Rice straw return also enhanced the contents of microbial biomass C （MBC） and microbial biomass N （MBN） in the NPKS plot by 7.76% for MBC and 31.42% for MBN compared with the CK plot, and 12.66% for MBC and 15.07% for MBN compared with the NPK plots, respectively. Application of only chemical fertilizer, however, increased MBN concentration, but decreased MBC concentration in soil.     

Influence of land management on soil nutrients and microbial biomass in the central loess plateau,northwest China

Loss of soil organic matter under cropping systems is often considered one of the most serious forms of agriculturally induced soil degradation. Therefore, understanding how to improve or maintain soil fertility is of importance for sustainable systems of agriculture. This study deals with the effects of succession fallow and fertilization combined with crop rotation on the chemical properties and microbial biomass of soil in the central Loess Plateau, China. In order to create a more uniform experimental environment and avoid the influence of different crop residues, wheat/potato (W/P) rotation was selected as a fertilization treatment. The results showed that with increasing fallow time organic carbon (Corg) and total nitrogen (TN) slightly increased, microbial biomass carbon (MBC) and MBC/Corg gradually decreased, and microbial biomass nitrogen (MBN) remained unchanged. However, only MBC/Corg among all the microbial parameters measured showed significant differences at various stages of fallow. Although there was a decrease in organic carbon and total nitrogen in the fertilized plots, MBC was not significantly different in the various fallow and fertilized plots except for one‐year‐old fallows, which had the highest MBC. MBN, MBC/Corg and MBN/TN in fertilized plots were higher than for plots at different stages of fallow. Fertilization can increase organic carbon, total nitrogen, MBC and MBN content (compared to the control). It was concluded that appropriate land management, such as fertilization combined with crop rotation and reducing one‐year‐old fallow, would be useful ways to improve or maintain soil fertility. Copyright © 2005 John Wiley & Sons, Ltd.     

不同有机物料培肥对渭北旱塬土壤微生物学特性及土壤肥力的影响

通过田间试验,研究了施用不同有机物料对渭北旱塬耕地土壤微生物学特性及土壤肥力的影响。结果表明,化肥与不同有机物料配合施用,土壤微生物学特性［微生物量碳（MBC）、微生物量氮（MBN）、脲酶、碱性磷酸酶］以及部分土壤养分状况（全氮、速效磷、速效钾、阳离子交换量）比单施化肥处理均得到进一步改善。化肥配施秸秆堆肥处理效果最明显,其中微生物量碳增加了41.96%,微生物量氮增加了54.55%,脲酶活性增加了19.71%,碱性磷酸酶活性增加了7.35%,速效磷增加了63.12%;而且土壤微生物量碳、氮与速效磷、阳离子交换量呈显著正相关,微生物商（qMB）、脲酶活性、碱性磷酸酶活性与全氮、速效氮、速效钾含量呈显著相关;同时SMBC、SMBN、qMB等与作物产量密切相关（相关系数分别为0.85,0.74,0.82）。因此,化肥配施秸秆堆肥处理在渭北旱地雨热条件下对于全面提升土壤质量具有重要的意义;同时该区域土壤中微生物量碳氮与土壤养分状况、作物产量具有很好的一致性,可以表征土壤肥力状况及生产力水平。     

Soil compaction and forest floor removal reduced microbial biomass and enzyme activities in a boreal aspen forest soil

Soil enzymes are linked to microbial functions and nutrient cycling in forest ecosystems and are considered sensitive to soil disturbances. We investigated the effects of severe soil compaction and whole-tree harvesting plus forest floor removal (referred to as FFR below, compared with stem-only harvesting) on available N, microbial biomass C (MBC), microbial biomass N (MBN), and microbial biomass P (MBP), and dehydrogenase, protease, and phosphatase activities in the forest floor and 0–10 cm mineral soil in a boreal aspen (Populus tremuloides Michx.) forest soil near Dawson Creek, British Columbia, Canada. In the forest floor, no soil compaction effects were observed for any of the soil microbial or enzyme activity parameters measured. In the mineral soil, compaction reduced available N, MBP, and acid phosphatase by 53, 47, and 48%, respectively, when forest floor was intact, and protease and alkaline phosphatase activities by 28 and 27%, respectively, regardless of FFR. Forest floor removal reduced available P, MBC, MBN, and protease and alkaline phosphatase activities by 38, 46, 49, 25, and 45%, respectively, regardless of soil compaction, and available N, MBP, and acid phosphatase activity by 52, 50, and 39%, respectively, in the noncompacted soil. Neither soil compaction nor FFR affected dehydrogenase activities. Reductions in microbial biomass and protease and phosphatase activities after compaction and FFR likely led to the reduced N and P availabilities in the soil. Our results indicate that microbial biomass and enzyme activities were sensitive to soil compaction and FFR and that such disturbances had negative consequences for forest soil N and P cycling and fertility.     

Natural nitrogen-15 abundance and carbohydrate content and composition of organic matter particle-size fractions from a sandy soil

Sandy soil samples collected from under a woody/grass savanna in the Lamto experimental area (6°13N, 5°20W; Côte dIvoire, West Africa), were fractionated according to particle size with the aim of measuring the natural abundance of ¹⁵N and determining the contents and composition of hydrolysable carbohydrates of soil organo-mineral particles for a better understanding of the contribution of each individual fraction to the soil function. The contributions of the fractions <20 m to the total pool of organic matter were 77% for C and 84% for N. Larger amounts of carbohydrates were found in the clay and silt fractions (3,784–6,043 g g^–1 soil). The carbohydrate composition indicated that microbe-derived carbohydrates [e.g. galactose (Gal) and mannose (Man)] accumulated preferentially in the fine fractions while plant-derived sugars [e.g. arabinose (Ara) and xylose (Xyl)] were dominant in coarse fractions. A negative relationship was observed between C:N ratio and ¹⁵N natural abundance on the one hand, and on the other hand between C:N and (Gal+Man):(Ara+Xyl), Man:(Ara+Xyl) and Man:Xyl ratios, clearly indicating that the chemistry of the organic materials of the particle-size fractions reflects a change from soil chemistry dominated by plant materials to that dominated by microbial biomass and metabolites. The contribution of a given fraction to soil microbial activity is controlled by the quality or quantity of associated soil organic matter, its microbial biomass and also by the accumulation of microbial-derived carbohydrates which can be resynthesized or recycled.     

Microbial response of an acid forest soil to experimental soil warming

 Effects of increased soil temperature on soil microbial biomass and dehydrogenase activity were examined on organic (O) horizon material in a low-elevation spruce-fir ecosystem. Soil temperature was maintained at 5  °C above ambient during the growing season in the experimental plots, and soil temperature, moisture, microbial biomass, and dehydrogenase activity were measured during the experiment. An incubation study was also conducted under three temperature regimes, 5, 15, and 25  °C, and under four moisture regimes of 20, 120, 220, and 320% to further evaluate these environmental factors on dehydrogenase activity and microbial biomass. Soil moisture content and microbial biomass controls were significantly lower (30% and 2 μg g^–1 soil, respectively) in the heated plots during the treatment period, suggesting that moisture content was important in controlling microbial biomass. In the incubation study, temperature appeared more important than moisture in controlling microbial biomass and dehydrogenase activity. Increasing temperature between 5  °C and 25  °C resulted in significant decreases in microbial biomass and dehydrogenase activity. Received: 7 August 1998     

Soil organic matter quantity and quality in mountain soils of the Alay Range, Kyrgyzia, affected by land use change

 Changes in soil management practices influence the amount, quality and turnover of soil organic matter (SOM). Our objective was to study the effects of deforestation followed by pasture establishment on SOM quantity, quality and turnover in mountain soils of the Sui Checti valley in the Alay Range, Kyrgyzia. This objective was approached by analysis of total organic C (TOC), N, lignin-derived phenols, and neutral sugars in soil samples and primary particle-size soil fractions. Pasture installation led to a loss of about 30% TOC compared with the native Juniperus turkestanica forests. The pasture soils accumulated about 20% N, due to inputs via animal excrement. A change in land use from forest to pasture mainly affected the SOM bound to the silt fraction; there was more microbial decomposition in the pasture than in the forest silt fraction, as indicated by lower yields of lignin and carbohydrates, and also by a more advanced oxidative lignin side-chain oxidation and higher values of plant : microbial sugar ratios. The ratio of arabinose : xylose was indicative of the removal of carbohydrates when the original forest was replaced by pasture, and we conclude that this can be used as an indicator of deforestation. The accumulation of lignin and its low humification within the forest floor could be due to the extremely cold winter and dry summer climate. Received: 10 March 1999     

废弃农耕地自然演替过程中微生物群落与土壤特性间的关系

The changes of microbial biomass carbon (MBC) and nitrogen (MBN) and microbial community in the topsoil of the abandoned agricultural land on the semi-arid Loess Plateau in China during the natural succession were evaluated to understand the relationship between microbial community and soil properties. MBC and MBN were measured using fumigation extraction, and microbial community was analyzed by the method of fatty acid methyl ester (FAME). The contents of organic C, total N, MBC, MBN, total FAME, fungal FAME, bacterial FAME and Gram-negative bacterial FAME at the natural succession sites were higher than those of the agricultural land, but lower than those of the natural vegetation sites. The MBC, MBN and total FAME were closely correlated with organic C and total N. Furthermore, organic C and total N were found to be positively correlated with fungal FAME, bacterial FAME, fungal/bacterial and Gram-negative bacterial FAME. Natural succession would be useful for improving soil microbial properties and might be an important alternative for sustaining soil quality on the semi-arid Loess Plateau in China.     

Variations in soil microbial biomass and N availability due to residue and tillage management in a dryland rice agroecosystem

Seasonal changes in the levels of soil microbial biomass C (MBC) and N (MBN), N-mineralization rate and available-N concentration were studied in rice–barley supporting tropical dryland (rainfed) agroecosystem under six combinations of tillage (conventional, minimum and zero tillage) and crop residue manipulation (retained or removed) conditions. Highest levels of soil MBC and MBN (368–503 and 38.2–59.7 μg g⁻¹, respectively) were obtained in minimum tillage residue retained (MT+R) treatment and lowest levels (214–264 and 20.3–27.1 μg g⁻¹, respectively) in conventional tillage residue removed (CT−R, control) treatment. Along with residue retention tillage reduction from conventional to zero increased the levels of MBC and MBN (36–82 and 29–104% over control, respectively). The proportion of MBC and MBN in soil organic C and total N contents increased significantly in all treatments compared to control. This increase (28% in case of C and 33% N) was maximum in MT+R and minimum (10% for C and N both) in minimum tillage residue removed (MT−R) treatment. In all treatments concentrations of N in microbial biomass were greater at seedling stage, thereafter these concentrations decreased drastically (21–38%) at grain-forming stage of both crops. In residue removed treatments, N-mineralization rates were maximum during the seedling stage of crops and then decreased through the crop maturity. In residue retained treatments, however, N-mineralization rates were lower than in residue removed treatments at seedling stage of both crops. At grain-forming stage in all instances the N-mineralization rates in residue retained treatments considerably exceeded the rates in corresponding residue removed treatments. Tillage reduction and residue retention both increased the proportion of organic C and total N present in soil organic matter as microbial biomass. Microbial immobilization of available-N during the early phase of crops and its pulsed release later during the period of greater N demand of crops enhanced the degree of synchronization between crop demand and N supply. The maximum enhancement effects were recorded in the minimum tillage along with residue retained treatment. In the dryland agroecosystem studied, two management practices in combination proved more advantageous than either practice alone in maintaining soil fertility levels. For soil fertility amelioration in dryland agroecosystems with least dependence upon chemical fertilizer input, post-harvest retention of about 20 cm shoot biomass (accounting for 25–40% aboveground biomass) of previous crop and its incorporation in soil through minimum tillage in the succeeding crop is suggested, especially in the case of cereal.     

Effect of land management and soil texture on seasonal variations in soil microbial biomass in dry tropical agroecosystems in Tanzania

Soil microbes are an essential component of most terrestrial ecosystems; as decomposers they are responsible for regulating nutrient dynamics, and they also serve as a highly labile nutrient pool. Here, we evaluated seasonal variations in microbial biomass carbon (MBC) and nitrogen (MBN) as well as microbial activity (as qCO₂) for 16 months with respect to several factors relating to soil moisture and nutrients under different land management practices (plant residue application, fertilizer application) in both clayey (38% clay) and sandy (4% clay) croplands in Tanzania. We observed that MBC and MBN tended to decrease during the rainy season whereas they tended to increase and remain at high levels during the dry season in all treatment plots at both of our test sites, although soil moisture did not correlate with MBC or MBN. qCO₂ correlated with soil moisture in all treatment plots at both sites, and hence soil microbes act as decomposers mainly during the rainy season. Although the effect of seasonal variation of soil moisture on the dynamics of MBC, MBN, and qCO₂ was certainly greater than that attributable to plant residue application, fertilizer application, or soil texture, plant residue application early in the rainy season clearly increased MBC and MBN in both clayey and sandy soils. This suggests that plant residue application can help to not only counter the N loss caused by leaching but also synchronize crop N uptake and N release from soil microbes by utilizing these microbes as an ephemeral nutrient pool during the early crop growth period. We also found substantially large seasonal variations in MBC and MBN, continuously high qCO₂, and rapid turnover of soil microbes in sandy soil compared to clayey soil. Taken together, our results indicate that soil microbes, acting as both a nutrient pool and decomposers, have a more substantial impact on tropical sandy soil than on clayey soil.     

Responses of microbial biomass and respiration of soil to topography,burning, and nitrogen fertilization in a temperate steppe

Temporal dynamics of microbial biomass and respiration of soil and their responses to topography, burning, N fertilization, and their interactions were determined in a temperate steppe in northern China. Soil microbial indices showed strong temporal variability over the growing season. Soil microbial biomass C (MBC) and N (MBN) were 14.8 and 11.5% greater in the lower than upper slope, respectively. However, the percentage of organic C present as MBC and the percentage of total N present as MBN were 16.9 and 26.2% higher in the upper than lower slope, respectively. Neither microbial respiration (MR) nor metabolic quotient (qCO₂) was affected by topography. Both MBC and MBN were increased by burning, on average, by 29.8 and 14.2% over the growing season, and MR and qCO₂ tended to reduce depending on the sampling date, especially in August. Burning stimulated the percentage of organic C present as MBC and the percentage of total N present as MBN in the upper slope, but did not change these two parameters in the lower slope. No effects of N fertilization on soil microbial indices were observed in the first growing season after the treatment. Further research is needed to study the long-term relationships between changes in soil microbial diversity and activity and plant community in response to burning and N fertilization.     

Variations of soluble organic nitrogen and microbial nitrogen in deciduous forest soils along an urban-rural gradient

Soluble organic N and microbial N pools in terrestrial ecosystems have been less studied than those of inorganic N. Therefore, cross-system variation in their pool sizes and seasonal dynamics, both absolute and relative to inorganic N pools, needs to be quantified so that their ecological importance in different ecosystems can be evaluated. We measured extractable soil organic N (ESON), microbial biomass N (MBN), and the net production rates of ESON in red oak-dominated remnant forests, along an urban-rural gradient in the New York City metropolitan area. We were interested in (1) determining the seasonal dynamics of ESON and MBN, and (2) examining whether the contrasts in land use (urban, suburban, rural) surrounding these forest remnants were associated with different amounts of ESON and MBN. This field-based study was conducted continuously for 16 months. Yearly average ESON concentrations ranged from 60 to 140 mg kg⁻¹ soil organic matter (SOM), 3-4 times those of inorganic N, and average MBN ranged from 600 to 1100 mg kg⁻¹ SOM. There was a considerable MBN increase in spring in all plots across the gradient. The average increase expressed on an areal basis (to a depth of 7.5 cm) ranged from 1.75 to 4.19 g N m⁻². The N incorporated into the microbial biomass in spring was gradually released later in the growing season (the mean MBN decrease ranged from 1.11 to 3.82 g N m⁻²). The spring MBN increase could be an important retention mechanism for conserving soil inorganic N when plant N uptake may be low. The amplitude in the seasonal dynamics of ESON was far less than that of inorganic N, as was that of net ESON production rates when compared to net N mineralization. These suggest a closer coupling of plant N uptake with inorganic N, much more than with ESON. Both ESON and MBN were significantly higher in rural soils than in urban soils, and both concentrations were positively correlated with SOM content. The variation in ESON, MBN, or SOM associated with this urbanization gradient suggests that the form of N exported, the plant N budget and soil N retention mechanisms may be differentially affected by urban, suburban and rural land uses.     

Seasonal variations in soil microbial biomass content and soil neutral sugar composition in grassland in the Japanese Temperate Zone

We investigated seasonal variations in the soil microbial biomass N content (MBN) and the soil neutral sugar composition from April 1995 to September 1996 in cattle-grazed pasture in a Japanese Andisol. MBN showed seasonal variation, gradually increasing from April to August, and decreasing rapidly by the end of August when soil moisture content decreased. Soil inorganic-N increased after fertilization and cattle pasturage. Seasonal variations in seven types of neutral sugar-C contents in the soil were observed. However, the trends in seasonal variation for sugars derived from plant materials were different from one another and from those derived from microbial products. Seasonal variation trends in soil neutral sugar-C were similar to that of MBN, with a sharp decrease in midsummer and a subsequent increase in autumn probably due to dence pasture plant growth. Furthermore, galactose-C (Gal), rhamnose-C (Rham) and ribose-C (Rib) that originated mainly from microbes showed positive correlations with MBN, but the other forms of soil neutral sugar-C did not. It was concluded that the composition of the soil organic matter accumulated in grassland varied seasonally, and that these seasonal variations can be investigated by MBN or by soil neutral sugar composition.     

添加秸秆碳源对土壤微生物生物量和原生动物丰富度的影响

为了研究引入秸秆碳源对根结线虫(Meloidogyne spp.)病害严重土壤中微生物生物量和原生动物的影响, 以番茄为供试作物, 设置4个梯度的小麦秸秆添加量[CK(0 g·kg^-1), 1N(2.08 g·kg^-1)、2N(4.16 g·kg^-1)和4N(8.32 g·kg^-1)], 研究不同种植时间(6个月和4个月)下土壤微生物生物量碳、氮和原生动物丰度的变化。研究结果表明: 添加秸秆对微生物生物量碳、氮和原生动物丰富度有明显促进作用, 添加的秸秆量越多, 这种促进作用越明显。不同秸秆添加量处理中, 微生物生物量碳、氮和原生动物丰度为: 4N>2N>1N>CK。秸秆对原生动物的群落结构也有显著影响, 在各处理中, 鞭毛虫和肉足虫占有绝大比例, 分别占总丰度的29.44%和66.19%, 纤毛虫仅占4.37%。在相同添加秸秆量条件下, 土壤原生动物丰度随种植时间的延长而提高, 而微生物生物量碳、氮量随种植时间的延长而降低。而在种植时间相同条件下, 随着秸秆量的增加土壤微生物生物量碳、氮量和微生物生物量碳氮比和原生动物总丰度相应增加。     

Responses of soil chemical and microbial indicators to conservational tillage versus traditional tillage in the North China Plain

This study compared the responses of soil chemical and microbial indicators to the conservational tillage (CT) versus traditional tillage (TT) in a Haplic Cambisol in the North China Plain (NCP). These indicators included soil organic C (SOC), soil total N (STN), soil available P (SAP), cation exchange capacity (CEC), exchangeable Ca²⁺ and Mg²⁺, microbial biomass C (MBC), microbial biomass N (MBN), alkaline phosphomonoesterase (AP), β-glucosidase, N-acetyl-β-glucosaminidase (NAG), nitrate reductase (NR), protease, urease and the geometric mean of the assayed enzymes (GMea). Our results showed that almost all investigated parameters, except the contents of CEC, Ca²⁺, Mg²⁺ and the ratios of GMea/MBN and C/N, were significantly higher under the CT (no-till, NT and reduced-till, RT) than those under the TT, whilst the crop yield was not significantly affected by tillage treatments. Principle component analysis (PCA) showed that the first and second component explained 67.2% and 16.6% of the total variation, respectively. The first component was significantly correlated with GMea, MBC, MBN and β-glucosidase, and effectively discriminated soils under the NT or RT from those under the TT. Our results indicated that the 6-year CT improved the quality of the Haplic Cambisol by enhancing its chemical and microbial properties, whilst GMea, MBC, MBN and β-glucosidase were among the most effective indicators for monitoring these improvements.     

巴西东北部地区土地退化对土壤微生物量和活性的影响

Land degradation causes great changes in the soil biological properties.The process of degradation may decrease soil microbial biomass and consequently decrease soil microbial activity.The study was conducted out during 2009 and 2010 at the four sites of land under native vegetation(NV),moderately degraded land(LDL),highly degraded land(HDL) and land under restoration for four years(RL) to evaluate changes in soil microbial biomass and activity in lands with different degradation levels in comparison with both land under native vegetation and land under restoration in Northeast Brazil.Soil samples were collected at 0-10 cm depth.Soil organic carbon(SOC),soil microbial biomass C(MBC) and N(MBN),soil respiration(SR),and hydrolysis of fluorescein diacetate(FDA) and dehydrogenase(DHA) activities were analyzed.After two years of evaluation,soil MBC,MBN,FDA and DHA had higher values in the NV,followed by the RL.The decreases of soil microbial biomass and enzyme activities in the degraded lands were approximately 8-10 times as large as those found in the NV.However,after land restoration,the MBC and MBN increased approximately 5-fold and 2-fold,respectively,compared with the HDL.The results showed that land degradation produced a strong decrease in soil microbial biomass.However,land restoration may promote short-and long-term increases in soil microbial biomass.     

不同种植方式对北疆绿洲土壤养分和生物学性状的影响

长期种植不同作物对土壤的理化性质与生物学性状有很大影响。本研究针对北疆绿洲灰漠土几种典型作物种植方式,研究了不同作物长期种植对土壤养分、酶活性、微生物量碳氮以及土壤呼吸的影响。结果表明:长期种植不同作物使土壤表层(0～20cm)与亚表层(20～40cm)的土壤肥力性质产生明显分异,表层土壤的肥力水平明显高于亚表层;土壤pH则表层低于亚表层,而不同种植方式对灰漠土pH无显著影响。长期种植不同作物对土壤主要生物过程关键酶活性、土壤呼吸等土壤生物性状有明显影响,磷酸酶、脲酶、β-葡萄糖苷酶、多酚氧化酶、纤维素酶在不同种植方式下差异显著,小麦/油葵轮作10年(WSR)方式下上述5种酶活性均较高。土壤微生物量碳氮随棉花连作年限的延长而显著下降,且玉米连作10年(CrM)、小麦连作10年后棉花连作10年(WCtR)、棉花连作15年(CtM)和小麦/油葵轮作10年的土壤微生物量碳氮比分别为6.31、6.02、5.83和3.53。不同种植方式下土壤呼吸在120h内的变化均为先下降后趋于平稳,不同种植方式下的土壤呼吸商之间呈显著性差异,WSR呼吸商最高,平均20.54μg(CO2-C)·mg-1(Bc)·h-1,WCtR最低,平均14.01μg(CO2-C)·mg-1(Bc)·h-1。作物长期连作后与绿肥作物轮作,有利于提高土壤生物活性,是一种较好的种植方式。