鸡粪与化肥不同配比对杨树苗根际土壤酶和微生物量碳、氮变化的影响

为比较鸡粪有机肥与化肥以不同比例配施对杨树苗根际土壤环境的作用效果,通过盆栽试验,研究了N100（尿素提供100%的氮）、 M10N90（鸡粪和尿素分别提供10%和90%的氮)、 M30N70（鸡粪和尿素分别提供30%和70%的氮）和M50N50（鸡粪和尿素各提供50%的氮）等处理对一年生杨树苗根际土壤酶活性、 微生物量碳（SMBC）和微生物量氮（SMBN）的影响。结果表明, 同N100处理相比,M30N70处理的杨树苗根际土壤脲酶、 碱性磷酸酶、 过氧化氢酶和蔗糖酶活性分别提高14.41%~26.49%、 13.87%~27.93%、 12.07%~27.08%和29.17%~46.43%; 根际SMBC、 SMBN含量分别提高63.00%~76.62%、 40.01%~90.38%。在杨树苗的年生长过程中,根际土壤脲酶、 碱性磷酸酶、 过氧化氢酶和蔗糖酶活性均在施肥后120 d 最高; 而根际SMBC和SMBN含量分别在施肥后90 d和30 d达到最大值。随着杨树苗的生长发育,SMBN含量逐渐降低,而在落叶期有一定的回升。与M30N70处理相比,M10N90和M50N50处理对杨树苗根际土壤酶和微生物量碳、 氮的影响较小。综合分析认为,鸡粪有机肥与化肥以3∶7比例配施对杨树苗根际区域生态环境的改善效果最佳。     

有机肥施用模式对蔬菜产量、土壤化学性质及微生物的影响

在广州市蔬菜集约化种植区连续进行了6茬菜心试验,探讨有机肥施用模式[施无机肥(CK)、无机肥配施国产生物有机肥(BM)、无机肥配施腐殖酸(HA)、无机肥配合淋施复合芽孢杆菌剂(BSP)、无机肥配施腐殖酸并淋施复合芽孢杆菌剂(HA+BSP)、无机肥配施水沤腐熟鸡粪并淋施复合芽孢杆菌剂(CM+BSP)、无机肥配施复合芽孢杆菌剂堆沤腐熟鸡粪(BSPCM)]对蔬菜产量、土壤化学性质及微生物的影响。结果表明,不同茬别菜心产量差别较大。连续6茬试验中,CM+BSP处理菜心产量均为最高且显著高于CK处理,BSPCM处理菜心产量仅次于CM+BSP处理。BM、CM+BSP、BSPCM处理能提高土壤pH,降低连作土壤的酸化风险。随着种植茬数的增加,CM+BSP处理土壤细菌、真菌和微生物总数持续增加,显著高于原始土壤和其他施肥处理,BSPCM处理次之。CK处理土壤中细菌、真菌及微生物总量均比原始土壤下降。在连作菜地蔬菜生产中,在施用无机肥基础上配施适量水沤腐熟鸡粪,并在蔬菜生长过程中淋施复合芽孢杆菌剂,不但可提高蔬菜产量,而且具有培肥、活化和改良土壤生物质量的作用,有利于减轻蔬菜连作障碍,实现集约化蔬菜种植的可持续发展。     

有机无机配施对水稻产量及氮肥残效的影响

为揭示有机无机配施条件下15N尿素在稻田土壤中的残效状况,在我国北方棕壤性水稻土上进行盆栽试验,结合土壤氮素供应及水稻对肥料氮的吸收利用,探寻最佳的施肥方式.设置空白(CK)、尿素(N)、秸秆+尿素(NS)、猪粪+尿素(NM)、稳定性尿素(NI)、秸秆+稳定性尿素(NIS)、猪粪+稳定性尿素(NIM)7个处理.结果 表...     

Enhancing phosphorus availability,soil organic carbon,maize productivity and farm profitability through biochar and organic–inorganic fertilizers in an irrigated maize agroecosystem under semi‐arid climate

Biochar amendments offer promising potential to improve soil fertility, soil organic carbon (SOC) and crop yields; however, a limited research has explored these benefits of biochar in the arid and semi‐arid regions. This two‐year field study investigated the effects of Acacia tree biomass‐derived biochar, applied at 0 and 10 t ha^?1 rates with farmyard manure (FYM) or poultry manure (PM) and mineral phosphorus (P) fertilizer combinations (100 kg P ha^‐1), on maize (Zea mays L.) productivity, P use efficiency (PUE) and farm profitability. The application of biochar with organic–inorganic P fertilizers significantly increased soil P and SOC contents than the sole organic or inorganic P fertilizers. Addition of biochar and PM as 100% P source resulted in the highest soil P (104% increase over control) and SOC contents (203% higher than control). However, maize productivity and PUE were significantly higher under balanced P fertilizer (50% organic + 50% mineral fertilizer) with biochar and the increase was 110%, 94% and 170% than 100%‐FYM, 100%‐PM and 100% mineral fertilizer, respectively. Maize productivity and yield correlated significantly positively with soil P and SOC contents These positive effects were possibly due to the ability of biochar to improve soil properties, P availability from organic–inorganic fertilizers and SOC which resulted in higher PUE and maize productivity. Despite the significant positive relationship of PUE with net economic returns, biochar incorporation with PM and mineral fertilizer combination was economically profitable, whereas FYM along biochar was not profitable due to short duration of the field experiments.     

秋施有机肥对土壤生物学、理化性状及玉米产量的影响

以黑龙江省海林农场白浆土为研究对象,在等氮量条件下（秋季有机肥配施来年春季无机肥）,设置100%有机肥（T₁）,30%有机肥+70%无机肥（T₂）,20%有机肥+80%无机肥（T₃）,100%无机肥（T₄）和不施肥（CK）5个处理,研究长期施肥对土壤生物学、理化性状以及玉米产量的影响。结果表明:玉米生育期内,土壤微生物数量呈先升高后降低的变化趋势,且施用高量有机肥有助于细菌和放线菌群落结构的形成,无机肥和不施肥适于真菌生长;有机无机肥配施处理在提高土壤酶活性方面占据绝对优势,脲酶和蔗糖酶活性始终显著高于其他处理,且有机肥较无机肥处理更能提高玉米生育期内土壤微生物生物量;有机无机肥配施可明显提高土壤全量和速效养分,培肥地力,单施效果稍差,且差异不大,而高量有机肥在降低土壤容重、增加总孔隙度百分比方面作用显著;两年玉米累计产量30%有机肥（T₂）最高,分别比100%有机肥（T₁）和100%无机肥（T₄）提高57.34%和4.59%,稳产并高产。总体来讲,30%有机肥+70%无机肥为最佳施肥模式。     

有机无机肥配施对麦-稻轮作系统土壤微生物学特性的影响

采用盆栽试验研究了不同比例有机无机肥配施对连续4茬麦-稻轮作后土壤微生物学特性的影响。结果表明,与对照相比,单施化肥处理促进了土壤微生物生物量碳、氮和微生物熵的增加,提高了土壤蔗糖酶、蛋白酶、脲酶活性,降低了过氧化氢酶活性,提高了放线菌的数量,但对土壤细菌、真菌数量的影响不明显;有机无机肥配施处理的土壤微生物生物量碳、微生物生物量氮、微生物熵、土壤酶活性及3大类土壤微生物数量显著高于单施化肥及对照处理;土壤微生物生物量碳、微生物生物量氮、微生物熵和3大类微生物数量随着有机肥配施比例的提高而增加,以配施30%有机肥处理的最高;土壤酶活性综合指数以配施20%有机肥处理的最高。可见,化肥配施有机肥特别是配施中高量有机肥更有利于改善土壤微生物学特性,提高土壤生产能力。     

Mineralization of Organic Nitrogen in Soils with Contrasting Fertility Was Regulated Differently by Addition of Biochar and Animal Manures

ABSTRACT

How to restore the soil fertility and productivity in a damaged and then reclaimed area with extremely low fertility is a big concern worldwide. To explore the method of soil restoration in the coal mining subsidence area, the effects of biochar application coupled with organic fertilizer (animal manures) on the process of organic nitrogen (N) mineralization were studied in a 149 days leaching experiment. Biochar were applied (wt/wt) at the rates of 0%, 1%, and 3%. Two organic fertilizers with different C/N ratio (chicken and sheep manures) were applied at the rate of 200 mg N·kg^?1 soil. A vegetable soil with high-fertility was used as the comparison. The results showed that when treated with chicken manure, the reclaimed soil had 11.13% lower mineralization potential and 20.00% lower inorganic nitrogen production from mineralization than the vegetable soil. Compared with the non-biochar treatment, biochar at both application rates decreased N leaching in chicken manure-treated reclaimed soil, i.e., by 21.49% (1% biochar) and 28.31% (3% biochar), respectively, whereas only high rate of biochar application decreased N leaching in chicken manure-treated vegetable soil by 8.10%. However, N leaching in sheep manure-treated reclaimed soil was unaffected by the biochar application. Thus, the effect of the biochar on the organic nitrogen mineralization was affected by both soil and organic fertilizer type.     

不同施肥管理措施对土壤碳含量及基础呼吸的影响

连续7年试验研究了施用15t/hm2和7.5t/hm2有机肥(包括EM堆肥、EM鸡粪肥和传统堆肥)、化肥和对照处理对土壤碳含量与基础呼吸的影响,结果表明:随有机肥施用量的提高,土壤可溶性碳、总有机碳、微生物生物量碳和土壤的基础呼吸随之增加。施用化肥可一定程度提高土壤可溶性碳、总有机碳、微生物生物量碳和土壤的基础呼吸。不同施肥措施对土壤有机碳、微生物生物量碳和土壤基础呼吸的影响趋势为EM堆肥处理>传统堆肥处理>化肥处理>对照,施肥对土壤微生物代谢商的影响趋势为EM堆肥处理<传统堆肥处理<化肥处理<对照。土壤微生物生物量碳与可溶性碳、总有机碳及土壤基础呼吸之间呈极显著正相关。土壤微生物代谢商与土壤可溶性碳、总有机碳、微生物生物量碳及基础呼吸之间呈极显著负相关。     

肥水蚓坑措施下不同施肥对土壤养分和山地苹果生产的影响

陕北黄土高原丘陵沟壑区是山地苹果重要产区,为提升山地果园土壤养分和果实品质及产量,以“肥水蚓坑”措施为基础,通过田间试验探讨不施肥、单施化肥、有机无机肥配施(牛粪+化肥、菇渣+化肥、牛粪+菇渣+化肥)等不同施肥对山地果园土壤养分、叶片营养、苹果产量与品质的影响。结果表明：(1)不施肥处理的土壤有机质含量和速效养分含量均低于施肥处理,其中,单施化肥处理低于有机无机肥配施处理,牛粪+菇渣+化肥处理的效果优于其他施肥处理。(2)施肥处理的苹果叶片不同生育期的全氮、磷、钾含量均显著高于不施肥对照,有机无机肥配施处理优于单施化肥处理。(3)不施肥处理与施肥处理的苹果果实硬度和果形指数无显著差异,施肥处理均可显著提高苹果产量和果实可溶性固形物、可溶性糖及维生素C含量,牛粪+菇渣+化肥优于其他处理,单施化肥的果实可滴定酸含量显著高于不施肥和有机无机肥配施。单施化肥的果实糖酸比与不施肥无显著差异,却显著低于有机无机肥配施。综上,“肥水蚓坑”结合有机无机肥配施可以减少化肥的施用,提高土壤养分含量,确保苹果提质增产,牛粪+菇渣+化肥(牛粪∶菇渣=1∶1)是山地苹果提质增产的优质管理模式,值得在陕北黄土丘陵...     

生物质炭与有机物料配施的土壤培肥效果及对玉米生长的影响

生物质炭作为一种多功能的土壤培肥材料被广泛应用,但其与传统有机物料的对比及配施研究还比较少。通过盆栽试验,研究了生物质炭与秸秆、发酵鸡粪单施及配施对壤质潮土和砂土养分含量、酶活性及玉米生长的影响,并采用主成分分析方法对3种有机物料的培肥效果进行综合评价。试验设6个处理,分别为不添加有机物料(CK)、添加生物质炭(BC)、小麦秸秆(WS)、发酵鸡粪(CM)、秸秆和生物质炭(WS+BC)、鸡粪和生物质炭(CM+BC)。研究结果表明,各处理均增加了砂土玉米生物量和株高,3种有机物料的提升幅度排序为:鸡粪生物质炭秸秆,鸡粪还可增加壤质潮土玉米生物量和株高。添加生物质炭和有机物料还可提高土壤有机质含量,其中生物质炭的提升幅度最大。此外,3种有机物料对土壤养分和酶活性的影响各异,单施鸡粪分别增加壤质潮土和砂土的碱解氮22.08%和26.67%,速效磷91.92%和53.65%,脲酶活性40.54%和36.94%;单施生物质炭分别增加壤质潮土和砂土速效磷83.52%和89.91%,速效钾79.38%和127.02%,过氧化氢酶活性3.41%和11.22%,却降低了土壤碱解氮含量,且与鸡粪配施后会抑制鸡粪中氮的有效性;单施秸秆分别增加壤质潮土和砂土速效钾49.48%和63.02%,β-葡糖苷酶活性51.86%和59.09%;生物质炭与鸡粪或秸秆配施可以更均衡地提升土壤肥力。通过主成分分析和相关分析发现,玉米生物量和株高与土壤氮、磷供应正变化的第2主成分(PC2)得分呈极显著正相关关系。因此,3种有机物料中,鸡粪对土壤氮、磷含量及相关酶活性影响最大;秸秆对土壤钾以及纤维素分解相关酶影响较大,而生物质炭对土壤肥力的提升作用更均衡,且土壤肥力综合得分最高。秸秆或鸡粪配施生物质炭可以更全面地提高土壤肥力。     

减氮配施抑制剂及鸡粪提高尿素氮在稻田土壤中的转化及利用

  【目的】  探究氮肥减量配施氮肥抑制剂和鸡粪的情况下土壤及肥料氮素供应和利用状况,及其对土壤肥力和水稻产量的影响,为我国东北地区水稻生产中提高氮肥利用效率、实现节肥增效提供理论基础。  【方法】  采用15N同位素示踪技术,盆栽试验设不施氮肥处理 (CK)、常规氮肥 (15N示踪尿素) 处理 (N)、80%尿素氮+20%鸡粪氮处理 (NM)、80%尿素氮+抑制剂处理 (NI)、80%尿素氮+抑制剂+20%鸡粪氮处理 (NIM)。测定不同生长时期来自于土壤及肥料中的铵态氮、微生物量氮含量及植株含氮量,收获时测定水稻产量。  【结果】  1) NI处理在土壤及肥料来源的铵态氮供应能力方面与N处理相当,抑制剂添加对氮肥减施有一定的补偿作用。在分蘖期和灌浆期,NM处理供氮能力优于无机氮肥处理。NIM处理在铵态氮和硝态氮供应能力方面效果最好。与N处理相比,NIM处理在水稻返青期、分蘖期和灌浆期土壤铵态氮含量分别提高了19.2%、66.3%和36.5%,硝态氮含量分别提高了13.9%、12.7%和17.3%,15NH₄+-N含量在分蘖期增加了14.59 mg/kg。2) 无机氮肥处理 (N、NI) 对土壤微生物量碳含量无显著影响,但添加鸡粪处理 (NM、NIM) 显著提高了返青期和灌浆期土壤微生物量氮含量 (P < 0.05)。与N处理相比,NIM处理在水稻返青期、分蘖期、灌浆期和成熟期土壤微生物量碳含量分别提高了32.61%、29.23%、53.46%和2.85%,微生物量氮含量分别提高了147.98%、22.97%、133.33%和24.63%,15N-微生物量氮含量在分蘖期增加了约22.56 mg/kg。3) 抑制剂及鸡粪添加均提高了水稻产量和生物量,NIM处理的水稻生物量、产量和吸氮量较N处理分别提高了83.59%、124.18%和46.66% (P < 0.05),土壤中肥料氮的残留量显著增加了56.48%,肥料氮的损失减少了约78.7%。NIM处理的氮素吸收利用率、氮肥农学效率等显著高于其他处理,抑制剂与鸡粪在提高肥料氮素利用率方面存在显著交互作用。  【结论】  在我国北方棕壤水稻土上,在尿素中添加抑制剂 (1%PPD+1%NBPT+2%DMPP) 或者用鸡粪替代20%的尿素均能改善土壤氮素供应,氮肥减量20%配施抑制剂和鸡粪不仅不会减产,还会在提高水稻产量的同时提高肥料利用率。从肥料氮释放及水稻吸收利用的角度综合考量,减少20%尿素投入,添加氮肥抑制剂,以及添加氮肥抑制剂的同时,用鸡粪替代20%的尿素的效果较好。     

不同有机肥对花生营养吸收、土壤酶活性及速效养分的影响

通过盆栽试验,研究等养分投入条件下,施用化肥与不同有机肥(猪粪、牛粪、鸡粪、麸酸有机无机复混肥)对花生营养吸收、土壤酶活性及速效养分的影响。结果表明,与化肥相比,施用有机肥脲酶活性提高6.2%～22.1%,磷酸酶活性提高7.9%～27.9%,过氧化氢酶活性提高45.1%～65.2%,分别以猪粪、鸡粪、麸酸有机无机复混肥最高,而转化酶活性各处理表现不一。施用有机肥较化肥促进了N、P、K养分向花生果仁转移累积,果仁吸N量、吸P量、吸K量、吸S量分别较化肥提高22.7%～78.0%、47.1%～74.5%、65.2%～91.6%、5.6%～61.2%,其NPK养分总吸收量以麸酸有机无机复混肥最高。施肥均提高了种植花生后的土壤N、P、K速效养分含量,施用麸酸有机无机复混肥还明显改善了土壤S素营养。     

不同培肥模式对茶园土壤微生物活性和群落结构的影响

以闽东地区红黄壤茶园定位实验地为对象,通过测定6种不同施肥处理土壤微生物学特性,研究不同培肥对土壤微生物特性和生物化学过程的影响,阐明各指标间的相互关系.结果表明,除了单施无机肥处理外,半量化肥+半量有机肥、全量有机肥、全量化肥+豆科绿肥以及半量化肥+半量有机肥+豆科绿肥等的培肥方式均不同程度提高了土壤有机质,可培养微生物数量,微生物量碳、氮含量及土壤酶活性,尤以半量无机肥+半量有机肥+豆科牧草的培肥模式增幅更为明显,而单施无机肥不利于微生物的生长、酶活性的提高和维持生态系统的稳定性.微生物群落磷脂脂肪酸(PLFAs)标记主成分分析显示,各种不同施肥方式使微生物群落结构发生改变.相关分析表明,微生物量与可培养微生物数量、微生物磷脂脂肪酸含量之间的相关性明显高于微生物量与各种酶活性之间的相关性,说明微生物数量大小对微生物群落结构的影响大于对酶活性功能的影响.研究也表明土壤各微生物指标能从不同方面反映土壤肥力水平,所以采用各种不同的方法能更客观地评价闽东地区茶园红黄壤质量的优劣.     

Effect of Integrated Nutrient Management on Soil Physical Properties and Crop Productivity under a Maize (Zea mays)–Mustard (Brassica campestris) Cropping Sequence in Acidic Soils of Northeast India

A five-year (2001/02–2006/07) field experiment was carried out on acidic clay loam soil classified as Typic Hapludalf with a maize–mustard crop sequence to study the effect of continuous application of nitrogen, phosphorus, and potassium (NPK) fertilizers alone and in combination with lime, farmyard manure (FYM), and biofertilizers on soil physical properties, soil organic carbon (SOC), soil microbial biomass carbon (SMBC), and crop yields on the hilly ecosystem of Meghalaya. Significant improvement in the soil physical conditions of the soil was observed under integrated application of organic manure and inorganic fertilizers. Addition of NPK fertilizers along with organic manure, lime, and biofertilizers increased soil organic carbon (SOC) content, aggregate stability, moisture-retention capacity, and infiltration rate of the soil while reducing bulk density. The SOC content under the treatment of 100% NPK + lime + biofertilizer + FYM was significantly greater (68.58%) than in control plots. Maize and mustard crop yields also significantly increased (4.73- and 21.09-folds, respectively) with continuous application of balanced inorganic (100% NPK) + lime + biofertilizer + FYM as compared to the control plots. However, crop yields drastically reduced under application of integrated nutrients without FYM as compared to the treatment with FYM application. Thus, the results suggest that integrated use of a balanced inorganic fertilizer in combination with lime and organic manure sustains a soil physical environment that is better for achieving higher crop productivity under intensive cropping systems in the hilly ecosystem of northeastern India.     

Effects of biochar compared to organic and inorganic fertilizers on soil quality and plant growth in a greenhouse experiment

Our contemporary society is struggling with soil degradation due to overuse and climate change. Pre‐Columbian people left behind sustainably fertile soils rich in organic matter and nutrients well known as terra preta (de Indio) by adding charred residues (biochar) together with organic and inorganic wastes such as excrements and household garbage being a model for sustainable agriculture today. This is the reason why new studies on biochar effects on ecosystem services rapidly emerge. Beneficial effects of biochar amendment on plant growth, soil nutrient content, and C storage were repeatedly observed although a number of negative effects were reported, too. In addition, there is no consensus on benefits of biochar when combined with fertilizers. Therefore, the objective of this study was to test whether biochar effects on soil quality and plant growth could be improved by addition of mineral and organic fertilizers. For this purpose, two growth periods of oat (Avena sativa L.) were studied under tropical conditions (26°C and 2600 mm annual rainfall) on an infertile sandy soil in the greenhouse in fivefold replication. Treatments comprised control (only water), mineral fertilizer (111.5 kg N ha^–1, 111.5 kg P ha^–1, and 82.9 kg K ha^–1), compost (5% by weight), biochar (5% by weight), and combinations of biochar (5% by weight) plus mineral fertilizer (111.5 kg N ha^–1, 111.5 kg P ha^–1, and 82.9 kg K ha^–1), and biochar (2.5% by weight) plus compost (2.5% by weight). Pure compost application showed highest yield during the two growth periods, followed by the biochar + compost mixture. biochar addition to mineral fertilizer significantly increased plant growth compared to mineral fertilizer alone. During the second growth period, plant yields were significantly smaller compared to the first growth period. biochar and compost additions significantly increased total organic C content during the two growth periods. Cation‐exchange capacity (CEC) could not be increased upon biochar addition while base saturation (BS) was significantly increased due to ash addition with biochar. On the other hand, compost addition significantly increased CEC. Biochar addition significantly increased soil pH but pH value was generally lower during the second growth period probably due to leaching of base cations. Biochar addition did not reduce ammonium, nitrate, and phosphate leaching during the experiment but it reduced nitrification. The overall plant growth and soil fertility decreased in the order compost > biochar + compost > mineral fertilizer + biochar > mineral fertilizer > control. Further experiments should optimize biochar–organic fertilizer systems.     

土壤中可溶性氮和pH对有机肥和化肥的短期响应

通过温室盆栽试验,施用猪粪堆肥(PC)、污泥堆肥(SC)、菜籽饼肥(CM)和无机化肥(IF)后,对土壤pH值和不同形态氮素的短期响应进行研究,探讨土壤可溶性无机氮、有机氮及土壤pH值之间的相互关系。结果表明,CM,PC和IF处理的pH值都呈现先快速下降后缓慢回升的趋势,但PC处理下降幅度较小;而SC处理和不施肥对照(CK)处理的pH值基本不变;不同施肥处理均提高了土壤中氮素水平,3种有机肥中以CM处理最显著地提高了土壤中总可溶性氮(TDN)、NH4+-N、NO3--N和可溶性有机氮(DON)浓度,PC处理的次之,SC处理的最弱,而IF处理与CM处理提高幅度相似。不同施肥处理中土壤可溶性氮表现出不同的短期响应,IF处理和CM处理的TDN分别经过11,13d的稳定期后迅速上升到一个较高的水平,至31d开始下降;PC处理和SC处理未出现突然升高的现象,而是前20d较稳定在一定的范围内波动,之后缓慢下降,总体与对照趋势一致。不同处理土壤中NH4+-N均呈下降趋势;IF处理、CM处理的NO3--N呈增加趋势,PC处理的呈先增加后降低趋势,而SC处理的呈缓慢下降趋势,结果说明不同有机肥在土壤中的转化存在明显的差异。相关性分析显示,CM处理、IF处理的土壤pH值与NH4+-N呈极显著正相关,而与NO3--N呈极显著负相关;除PC处理外,其他处理的DON含量与TDN具有显著正相关性,而PC处理的DON同时与NH4+-N、NO3--N呈显著负相关。     

有机无机肥料配合施用对滨海盐土土壤生物量态氮及土壤供氮特征的影响

采用盆栽试验研究海盐渍化土上蚕粪与无机氮肥（碳铵和尿素）的施用对土壤生物量态氮和土壤供氮特征的影响，指出（１）施肥均能明显地提高土壤生物量态碳和生物量态氮的含量，蚕粪与无机氮肥配合施用比化肥单施或蚕粪单施更能增加土壤生物量态氮含量。（２）土壤生物量态氮是土壤氮素转化的重要环节，也是土壤有效氮的重组成部分；（３）土壤生物量态氮的消长与壤的供氮特征关系极为密切，只要前期有强烈的微生物固持无机氮肥过程，     

Corn biomass,uptake and fractionation of soil phosphorus in five soils amended with organic wastes as P fertilizers

Abstract

Sustainable food production includes mitigating environmental pollution and avoiding unnecessary use of non-renewable mineral phosphate resources. Efficient phosphorus (P) utilization from organic wastes is crucial for alternative P sources to be adopted as fertilizers. There must be predictable plant responses in terms of P uptake and plant growth. An 18-week pot experiment was conducted to assess corn (Zea mays L.) plant growth, P uptake, soil test P and P fractionation in response to application of organic P fertilizer versus inorganic P fertilizer in five soils. Fertilizers were applied at a single P rate using: mono-ammonium phosphate, anaerobically digested dairy manure, composted chicken manure, vegetable compost and a no-P control. Five soils used varied in soil texture and pH. Corn biomass and tissue P concentrations were different among P fertilizers in two soils (Warden and Quincy), with greater shoot biomass for composted chicken manure and higher tissue P concentration for MAP. Plant dry biomass ranged from highest to lowest with fertilizer treatment as follows: composted chicken manure?>?AD dairy?=?MAP?=?no-P control?=?vegetable compost. Soil test P was higher in soils with any P fertilizer treatment versus the no-P control. The loosely bound and soluble P (2.7?mg P kg^?1) accounted for the smallest pool of inorganic P fractions, followed by iron bound P (13.7?mg P kg^?1), aluminum bound P (43.4?mg P kg^?1) and reductant soluble P (67.9?mg P kg^?1) while calcium bound P (584.6?mg P kg^?1) represented the largest pool of inorganic P.     

有机无机氮肥配施下洛阳烟田土壤N2O排放特点及其控制因素

随着农田化肥使用量的逐年增加和土壤退化问题日趋严重,农田温室气体排放关注度持续提高,为研究旱作植烟土壤N_2O排放特征及影响机理,设置6个田间试验处理,分别为CK0(不施肥处理)、CK1(100%无机氮)、T1(50%无机氮+50%饼肥氮)、T2(50%无机氮+50%羊粪肥氮)、T3(25%无机氮+75%饼肥氮)、T4(25%无机氮+75%羊粪肥氮),各处理施氮量均为45 kg/hm²,烟田施用基肥后起142天内测量不同处理土壤N_2O排放通量、硝态氮、铵态氮含量、根层温度和含水率。结果表明:(1)基肥施入后的3~7天内,土壤N_2O排放通量进入高峰,无机肥处理和有机无机肥配施处理的高峰期分别可维持20,9天,追肥后3天再次出现排放峰并持续9天,随后伴随烟株的生长发育,烟地N_2O排放通量逐渐趋向稳定。(2)基肥施用后仅1个月内N_2O累积排放量可达到总排放量的27.4%~32.6%;处理间N_2O排放量和排放系数均表现为无机>有机+无机(1∶1)>有机+无机(3∶1),无机肥配施有机肥明显降低了肥料中氮素以N_2O形态的损失量;与无机肥相比,T1和T2烟叶产量分别增加9.44%和6.37%,T1、T2、T3和T4处理的N_2O排放强度有着不同程度的降低。(3)主成分分析结果显示,在不施肥烟地中0—5 cm土壤温度和含水率是N_2O排放通量主导因子,利用相关性分析此环境下温度和水分分别与N_2O排放通量间呈现显著和极显著正相关关系;施肥后土壤铵态氮含量和土壤含水率是烟地N_2O排放通量的主导因子且相关性分析均呈现极显著正相关关系。综上,旱地植烟土壤N_2O排放受氮肥种类影响较大,施肥后N_2O排放通量对土壤温度响应减弱,主要受土壤铵态氮含量和含水量的影响;在总氮量相同情况下,有机无机肥配施比例为1∶1时明显降低土壤N_2O排放并提高了产量,该比例饼肥和羊粪肥处理分别将烟地N_2O排放强度降低20.4%和23.7%。     

不同有机肥源对土壤微生物生物量及花生产量的影响

通过盆栽试验,采用平板计数法和DGGE分析法,研究施用化肥与不同来源的有机肥对土壤微生物生物量及花生产量的影响.结果表明,施肥均显著提高了花生的经济产量与生物产量,其中以施用麸酸有机复混肥处理最高;土壤中细菌、真菌、放线菌总量以施用鸡粪处理最高,其他处理差别不大;土壤微生物总DNA提取、PCR扩增及其产物DGGE分析表明,施用各品种有机肥较不施肥与施用化肥促进了土壤某些微生物量的提高,而施用不同有机肥品种促使不同种类微生物量的提高.故不同有机肥源对土壤微生物生物量乃至其多样性特征均产生影响.