峡江鱼道过鱼效果初步评价

鱼道是解决拦河筑坝阻隔鱼类洄游的重要手段之一。为了满足兴建峡江水利枢纽工程后洄游鱼类上溯的要求,保护赣江水生生态环境的完整性,减缓工程对鱼类种群遗传交流的影响,针对峡江水利枢纽实际情况制定了鱼道过鱼方式。鱼道采用垂直竖缝式结构设计,由上游鱼道(出口段)、坝体过鱼孔口、下游主、副鱼道(进口段)、集鱼系统及连接段组成,设计主要过鱼季节为4-7月,流速0.7~1.2 m/s。采用仪器监测与人工观察相结合的方式对峡江鱼道过鱼效果进行了初步监测。监测结果显示:2017年监测到过鱼总数67.8万尾,其中上行占41.67%;期间共监测到22种鱼类,共计4目、7科、18属,以小型鱼类为主,如贝氏(Hemiculter bleekeri)、三角鲂( Megalobrama terminalis)、银鲴(Xenocypris argentea)、银鮈(Squalidus argentatus);不同季节过鱼数量不同,其中以二季度最多,占总数49.17%,过鱼数量以7月最多;过鱼数量昼夜差别大,以上午10:00至下午16:00是过鱼数量最多;与国内其他鱼道相比,峡江鱼道过鱼数量较多,过鱼效果显著。     

长洲水利枢纽鱼道功能的初步研究

鱼道是解决水坝阻隔鱼类上溯的重要途径之一,过鱼效果的监测是其科学运行的重要环节。２011年和2012年４－６月对试运行阶段的长洲水利枢纽鱼道进行了监测采样;共计采集到鱼类３０种,主要优势种类为银飘鱼（Pseudolaubuca sinensis）、餐（Hemiculter leucisclus）、瓦氏黄颡鱼（Pelteobagrus fulvidraco）、赤眼鳟（Spualiobarbus curriculus）、鲮（Cirrhinus molitorella）、日本鳗鲡（Anguill japonica）,青鱼（Mylopharyngodon piceus）、草鱼（Ctenopharyngodon idellus）、鲢（Hypophthalmichthys）、鳙（Aristichthys mobilis）也有出现;体长6０～870ｍｍ的个体可以通过鱼道上溯;鱼道中鲢体重达1.５ｋｇ,鲤（Cyprinus carpio）、鲶（Schilbe intermedius）体重达到2.８ｋｇ,采集到最大规格的种类为鳡（Elopichthys bambusa）,体长达870ｍｍ,体重7061ｇ。监测数据表明,长洲水利枢纽鱼道具有较好的过鱼功能,但监测中未采集到西江重要的洄游性鱼类广东鲂（Megalobrama hoffmanni）。从不同批次监测到的鱼类种类组成及规格上分析,鱼道运行效果明显受水文变化的影响,其运行及效果监测需要充分考虑水文状况及西江鱼类的生态习性。     

流溪河水厂坝丹尼尔式鱼道运行效果初探

为检验流溪河水厂坝建立的丹尼尔式鱼道试点工程效果,在2015-2016年鱼道主要过鱼时段4-10月,利用张网法逐日监测。结果显示,鱼道取样共采集鱼类906尾,隶属于3目、8科、32属、34种,体长范围5～74cm,优势种为尼罗罗非鱼(Tilapia niloticus)(数量比51.10%)、歺又鱼(Hemiculter leucisculus)(16.23%)、唇鱼骨(Hemibarbus labeo)(8.17%)、银鮈(Squalidus argentatus)(6.73%)、东方墨头鱼(Garra orientalis)(1.55%),以定居型鱼类为主,未见河口洄游性鱼类。水温与上游水位是影响鱼类种类时间分布的主要环境因素。聚类分析表明,6月过鱼群体的种类和数量独成一类,与其他月份鱼类群体最不相似。虽然Shannon多样性指数在5月和6月分别为2.068和1.719,但总体偏低(1.451±0.535),而Margalef丰富度指数差别较小,表明不同鱼类对鱼道入口的感应存在种间差异,推测是由工程全段翻滚闸门下泄水流导致鱼道入口不易发现所致。研究表明,丹尼尔式鱼道试点工程效果较好,为该河段的绝大部分鱼类提供了上溯通道,对流溪河构建整体过鱼设施体系起到了良好的示范作用,有助于流溪河生态系统的健康与恢复。     

新疆某枢纽工程鱼道的设计

新疆某枢纽工程为低水头拦河建筑物,位于河流平原区,两岸地形平坦开阔。１１月－翌年３月,泄洪闸全部开启,河道基本为自然状态,鱼类可以自由通过;４－１０月,部分泄洪闸关闭,是本工程的过鱼季节。过鱼对象主要是新疆裸重唇鱼等需要短距离迁徙的裂鳆鱼类,兼顾草鱼、鲢等经济鱼类。综合考虑诱鱼能力、过鱼能力、鱼类适应程度、建造成本和运行维护等因素,其过鱼设施经过比选后选定为仿自然通道型鱼道。介绍了该工程仿自然通道型鱼道的论证与设计过程,为低水头拦河建筑物的过鱼设施设计提供借鉴。     

高坝鱼道工程设计案例分析

坝高１１６ｍ,最大水头６７.０ｍ,额定水头５３．５ｍ。主要过鱼季节为３－６月,兼顾过鱼季节为２月、７－１０月。综合考虑工程特性和过鱼对象生态习性,设计采用竖缝式池室结构型式鱼道,主要由进口段、槽身段、暗涵段、明渠段、过坝段、出口段、进口补水设施和观测研究室等部分组成,全长３５６６．３２５ｍ。共设置２个常用进口,２个备用进口,鱼道过鱼孔设计流速１．１ｍ／ｓ。介绍了鱼道设计技术路线及研究方法,提出了休息池、鱼道进口、补水设施和带集鱼池的观测研究室的设计思路。     

射频识别技术(RFID)在鱼道监测中的应用

过鱼效果的监测评价及相应技术优化是鱼道成功运行的保证。通过对比射频识别技术与其他水生生物监测技术之间的优缺点,从鱼类生理伤害度、监测精度、标签遗失率以及适用鱼类个体尺寸范围和监测成本等方面,阐述了射频识别技术在鱼道监测工作中的优越性。结合射频识别技术在鱼道内部通过效果、鱼道进口吸引效果和鱼道河流连通性修复方面的应用,概括了射频识别技术应用现状及其所包含的关键技术,加强射频识别技术的研究对鱼道监测、评价、优化具有重大意义。     

亚马逊流域玛代拉河Santo Antonio 鱼道设计与建设的启示

Santo Antonio过鱼设施位于亚马逊河最大支流玛代拉河下游,其设计规模宏大,在世界范围内鲜有,并且工程建设地所在区域水文环境和鱼类生物学特征等与我国长江较为类似,通过分析该过鱼设施,得出启示主要包括：（1）过鱼设施是为生物对象设计的水利工程,其设计和建设过程中必须由工程师和生物学家紧密合作;（2）吸引水流对过鱼设施的成败起关键性作用,虽然吸引水流系统需要加大投资并且运行过程中会损失大量的水能,但从确保过鱼设施效果而言是非常必要的;（3）物理模型对于鱼道进口位置,鱼道内部水力学特征的确定等具有重要参考价值,尤其在1:1局部模型内开展的生物学实验是细化和优化鱼道内部结构细节的有效方法;（4）卵石框(石笼）隔板的水池分隔方法兼具水池式鱼道与仿自然通道的双重特征,并且完工后还可以根据鱼道的运行情况灵活调整,非常方便;（5）进口处采用较高的人字闸门,即可适应尾水变化,又可与吸引水流系统相配合,创造出有效的吸引水流,一举多得;（6）全部采用大功率灯泡式水轮机是保护下行鱼类的有效措施;（7）监测系统和公众教育等配套设施的建设非常必要,有利于改进鱼道运行方式和提高过鱼效率。     

峡江水利枢纽鱼道过鱼效果的初步研究

为了解峡江水利枢纽鱼道过鱼效果,采用张网法和截堵法,于2019年10月、2020年1月、2020年4月、2020年8月对峡江水利枢纽鱼道的过鱼效果开展季度调查.鱼道内共采集鱼类4605尾,计42种,隶属于4目、9科、34属,以中小型鱼类为主,体长范围为2.3～48.5 cm,优势种为宽鳍鱲(Zacco platypus...     

我国过鱼设施现状分析及鱼道适宜性管理的关键问题

水电开发对于洄游性鱼类产生的负面影响最为直接,阻碍了其繁殖、索饵以及越冬等生命行为。在调查的25个水电项目中,有17个采取了鱼类保护措施,其中采取增殖放流措施的占82.4%,仅有1个项目修建了鱼道。介绍了“莱茵河-2000计划”和哥伦比亚河鱼道建设及运行效果。鱼道不单纯是洄游性鱼类的通道,更是实现大坝上下游物质与能量循环的生态廊道。国内开展鱼道适应性管理的关键问题是：建立鱼道长效运行与保障机制,对鱼道过鱼进行长效监测以及研究鱼道过鱼效果评价方法。     

一种鱼道进口诱鱼结构装置的试验研究

常规鱼道的研究和设计大多侧重于其内部结构和水流条件等方面,对鱼道进口位置布置及结构优化的研究相对较少,往往存在鱼类发现并进入鱼道较为困难的情况。通过建立鱼道进口设计的生态水力学指标,提出了某项目集诱鱼进口所需要满足的流速范围为0.47～1.30 m/s,将鱼类游泳能力与鱼道进口流态指标相结合,用标准三维κ-ε模型数值模拟的方式,对以往鱼道进口位置的选择原则进行了验证,提出了一种可控流量、流速的新型诱鱼补水装置,对鱼道进口结构进行优化。该诱鱼补水装置利用局部水流落差,上游发电下泄水流进行诱鱼补水,避免采用自库区压力管道引水而产生的消能问题,降低了鱼道运行的成本;同时降低机组满发或发电流量较高时河段内的流速,可达到灵活调控诱鱼水流进而诱导鱼类进入鱼道完成洄游的效果,最终完成鱼道进口的优化,提高过鱼效果。     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Rare serotype occurrence and PFGE genotypic diversity of Streptococcus agalactiae isolated from tilapia in China

Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

Purification and partial characterization of GtHs (cfLH and cfFSH) from Indian walking catfish (Clarias batrachus) (L.) and development of a homologous ELISA for cfLH

Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL^?1 (1.25 ng well^?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation.     

Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection

Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg^?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality).     

Protein and amino acid composition from the mantle of juvenile O ctopus vulgaris exposed to prolonged starvation

Protein and amino acid composition of the mantle of juvenile O ctopus vulgaris (Cuvier, 1797) during fasting for 27 days were determined. Average protein content of octopus mantle was of 711.19 ± 46.80 g kg^?1 DW, and it decreased with increasing fasting days. The non‐essential amino acids content was higher (486.18 ± 11.08 g kg^?1 protein) than essential amino acids (425.82 ± 9.15 g kg^?1 protein) at the start of the experiment (unstarved animals). The results suggest that the amino acid profile of the mantle where the most abundant amino acids are Arg, His, Lys, Gly, Leu and Pro could indicate a prolonged fasting condition (>20 days) or poor nutrition of O . vulgaris. This study supports the idea of using mantle for metabolic needs of starved O . vulgaris suggesting that the degradation pathway of amino acids to pyruvate and tricarboxylic acid cycle intermediates was favoured contrary to the degradation pathway of ketogenic amino acids. Special considerations should be taken concerning Thr, Ile, Ser, Ala, Asx (Asp, Asn), Glx (Glu, Gln) (because of their fast intake) and Lys and His (due to their stable contents) during a prolonged period of fasting.     

Imported ornamental fish are colonized with antibiotic‐resistant bacteria

There has been growing concern about the overuse of antibiotics in the ornamental fish industry and its possible effect on the increasing drug resistance in both commensal and pathogenic organisms in these fish. The aim of this study was to carry out an assessment of the diversity of bacteria, including pathogens, in ornamental fish species imported into North America and to assess their antibiotic resistance. Kidney samples were collected from 32 freshwater ornamental fish of various species, which arrived to an importing facility in Portland, Oregon from Colombia, Singapore and Florida. Sixty‐four unique bacterial colonies were isolated and identified by PCR using bacterial 16S primers and DNA sequencing. Multiple isolates were identified as bacteria with potential to cause disease in both fish and humans. The antibiotic resistance profile of each isolate was performed for nine different antibiotics. Among them, cefotaxime (16% resistance among isolates) was the antibiotic associated with more activity, while the least active was tetracycline (77% resistant). Knowing information about the diversity of bacteria in imported ornamental fish, as well as the resistance profiles for the bacteria will be useful in more effectively treating clinical infected fish, and also potential zoonoses in the future.     

Effect of iodophor disinfection of non‐hardened Salmo trutta eggs on their bacterial and fungus load

This study investigated the efficiency of iodophor disinfection (135 ppm active iodine for 15–30 min) of non‐hardened Salmo trutta eggs against different groups of bacteria and against fungus. Egg samples were taken from non‐disinfected and from disinfected eggs, microorganisms were cultured on specific nutrient media and their mass was measured by turbidimetric methods. Bacteria and fungus mass of non‐hardened eggs could be reduced but not eliminated by iodophor disinfection with 135 ppm active iodine for 15 min. The extent of reduction was 47–65% (Experiment 1). The efficiency of disinfection increased with disinfection time as the reduction in bacteria and fungus mass was 40–55% after 15 min and 58–74% after 30 min (Experiment 2). Disinfection efficiency of iodophor solution diluted in water (reduction 49–57%) and of iodophor solution diluted in sodium chloride solution iso‐osmolar to the oocytes (reduction 52–61%) was similar (Experiment 3). The reduction in bacteria and fungus mass was persistent as it was 39–72% lower in embryos deriving from disinfected eggs than in embryos deriving from non‐disinfected ones (Experiment 4). In conclusion, the tested disinfection method is inadequate to eliminate pathogens completely but it could positively influence immune defence of eggs and embryos.     

Influence of the antibacterial herbs, Solanum trilobatum, Andrographis paniculata and Psoralea corylifolia on the survival, growth and bacterial load of Penaeus monodon post larvae

The indiscriminate use of antibiotics and chemicals in shrimp hatcheries has led to biomagnification and that in turn could lead to rejection of a whole consignment. The application of the bioencapsulation technique as a tool for curative treatment in shrimp larvae was investigated. Herbs having antibacterial properties such as Solanum trilobatum, Andrographis paniculata and Psoralea corylifolia (methanolic extracts) were bioencapsulated in Artemia and fed to Penaeus monodon post larvae PL 1–25. The post larvae were reared in a medium inoculated with pathogenic bacteria such as Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Vibrio sp. Post larvae reared in the non-inoculated water and fed with non-enriched Artemia exhibited 90% survival, highest specific growth rate (12.43%) and reduced bacterial load. P. monodon reared in the bacterial inoculated water and fed with the non-enriched Artemia exhibited the lowest survival (10–30%), specific growth rate (8.42–9.1%) and increased bacterial load (2.86 × 10³ to 3.76 × 10⁵ cfu/g). The methanolic extracts of the herbs helped to increase survival and specific growth rate and reduced bacterial load in the P. monodon culture system. Among the three herbal extracts, P. corylifolia enriched Artemia fed post larvae showed the tendency to higher survival (>50%), growth rate (11.5 averaged) and low bacterial load (1.12 × 10⁵ cfu/g). This revised version was published online in August 2006 with corrections to the Cover Date.     

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Plasma estradiol-17 (E₂), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E₂ and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E₂ and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass.