Fast and versatile multiresidue method for the analysis of botanical insecticides on fruits and vegetables by HPLC/DAD/MS

A simple multiresidue method for screening analysis of 12 botanical insecticides used by organic farmers has been developed. The method involves a rapid and small-scale extraction procedure with acetonitrile. For all fruit and vegetable samples, there was no need for clean up. Rotenone, azadirachtin, ryanodines, and pyrethrins can be separated by high-performance liquid chromatography, quantified, and confirmed with a diode array detector (DAD) and atmospheric pressure chemical ionization mass spectrometry (APCI-MS) in the select ion-monitoring mode (SIM). The majority of pesticide recoveries for various fruits and vegetables were >70% in the concentration range from 0.01 to 5 mg/kg. The limit of quantitation for most of the pesticides was 0.01 mg/kg, with the majority of relative standard deviations (RSD) mostly below 10%.     

Analysis by hplc of ryanodine and dehydroryanodine residues on fruits and in ryania powdery wood.

A simple and rapid HPLC method to evaluate residues of the major ryanoids (ryanodine and dehydroryanodine) on three fruits (olives, apples, and pears) has been developed. The pesticides were extracted from the fruits with hexane and acetone solution (1:1, v/v). Cleanup was carried out with aminopropyl-bonded silica cartridges. This method is characterized by recovery >75%, precision <11% RSD, and sensitivity of 0.020 mg/kg. The method can also be used to determine the level of active ingredients in ryania powdery wood.     

岚皋县岩石、土壤和农产品中硒分布规律研究

[目的]探讨岚皋县岩石、土壤和农产品硒含量分布特征及其相互关系,揭示岚皋县岩石、土壤和农产品硒的分布规律.[方法]采集岚皋县岩石、土壤和农产品样本,测定样本中总硒含量.[结果]岚皋县岩石硒含量范围0.006～43.38 mg kg-1,均值4.15 mg kg-1,震旦系和寒武系地层岩石硒含量显著高于其他地层;土壤硒含...     

Isolation, characterization, and surfactant properties of the major triterpenoid glycosides from unripe tomato fruits

Various triterpenoid glycosides were extracted from whole unripe tomato fruits ( Lycopersicon esculentum cv. Cedrico), using aqueous 70% (v/v) ethanol to study their surfactant properties. Cation-exchange chromatography using a Source 15S column and subsequent semipreparative HPLC using an XTerra RP18 were employed to purify individual triterpenoid glycosides from the extract. The structure of the purified compounds was established by mass spectrometry and nuclear magnetic resonance spectroscopy. The furostanol glycoside tomatoside A (749 mg/kg of DW) and the glycoalkaloids alpha-tomatine (196 mg/kg of DW) and esculeoside A (427 mg/kg of DW) were the major triterpenoid glycosides present. Furthermore, minor amounts of a new dehydrofurostanol glycoside, dehydrotomatoside, were found. The critical micelle concentrations of the major triterpenoid glycosides, alpha-tomatine, tomatoside A, and esculeoside A, were determined as 0.099, 0.144, and 0.412 g/L, respectively. The results show that tomatoside A, and not the more well-known alpha-tomatine, is the predominant triterpenoidal surfactant in unripe tomato fruits.     

Determination of ETU in tomatoes and tomato products by HPLC-PDA: evaluation of cleanup procedures

An HPLC-PDA method for the determination of ethylenethiourea (ETU), the main degradation product of the organic fungicides ethylene bis(dithiocarbamate)s (EBDCs), in tomatoes and tomato products is reported. Solid-matrix liquid-liquid (l-l) partitioning and separatory funnel l-l partitioning for the cleanup were examined. The effect of salt addition, pH, and phase ratio on analyte recovery at the cleanup step was studied. It was found that solid-matrix l-l partitioning afforded higher precision and more selective separation of the analyte. According to the method proposed, the samples were extracted with methanol/water (3:1, v/v) and cleaned up on an Extrelut 20 column. ETU was eluted with dichloromethane and separated on a reversed phase HPLC column. For tomato products with degrees Brix > 20 further purification through silica cartridge was adopted. The method was validated over the following ranges of concentrations: 0.01-0.5 mg/kg for tomatoes, 0.01-0.1 mg/kg for tomato juice, and 0.05-0.25 mg/kg for tomato paste. The accuracy (recoveries > 70%) and the precision obtained (%RSD < 10%) were satisfactory.     

广州市蔬菜和菜地土壤砷含量及其健康风险研究

测定了广州市郊区菜地土壤(n=120)、菜地蔬菜(n=109)和市售蔬菜(n=237)中砷含量,结合广州居民蔬菜消费情况,分析了砷对广州居民的健康风险影响.结果表明:不同类型菜地土壤砷含量不同,为菜园土(12.94±9.78)mg/kg>水稻土(8.67±10.24)mg/kg>赤红壤(4.17±3.70)mg/kg,土壤质量主要属于二级标准以内;菜地蔬菜砷含量范围为ND～0.179 mg/kg,均值为(0.017±0.024)mg/kg;市售蔬菜砷含量范围为ND-0.314 mg/kg,含量变化为豆类(0.038±0.047)mg/kg)>根茎类(0.027±0.031)mg/kg>茄类(0.025±0.030)mg/kg>叶菜类(0.024±0.022)mg/kg>葱蒜类(0.019±0.025)mg/kg>瓜类(0.017±0.020)mg/kg,所有蔬菜均没有超过我国食品中砷的限量卫生标准(GB-4810-94).广州市居民从蔬菜中摄入的砷为0.045 mg/d,叶菜类和根茎类蔬菜是主要的贡献者.     

铁及其它矿质元素在苹果树不同器官中的分布

成龄苹果树果实采收期整株分析结果表明，单株总铁含量4915.78mg，叶、果、根、枝干的铁含量分布为1165.36mg、300.72mg、1500.38mg和1950.43mg，分别占单株总铁量的23.70%、6.12%、30.52%和39.66%。铁浓度以幼嫩根中最高，直径＜0.5cm根中达257.51mg／kg；其次为叶片，达214.09mg/kg；果实中最低，为18.27mg/kg。微量元素在叶中以铁浓度最高，在果实中以硼浓度最高，为29.26mg/kg；大量元素在叶中以氮浓度最高达18300mg/kg，在果实中以钾浓度最高，为6200mg/kg。     

Determination of acetamiprid,imidacloprid, and nitenpyram residues in vegetables and fruits by high-performance liquid chromatography with diode-array detection

Determination of 3 neonicotinoid insecticides, nitenpyram, imidacloprid, and acetamiprid, was studied. Vegetables and fruits were extracted with acetonitrile. The crude extract was passed through a weak anion-exchange cartridge (PSA). The effluent was subjected to silica gel cartridge. Imidacloprid and acetamiprid were eluted with 10 mL of 4:6 (v/v) acetone/hexane, followed by nitenpyram with acetone (20 mL). Pesticides were determined by HPLC with a C-18 column and diode-array detection system. Imidacloprid and acetamiprid were recovered at about 90% at the spike levels with 0.2 and 2 mg/kg in cucumber, potato, tomato, eggplant, Japanese radish, and grape. Nitenpyram was recovered at 64-80%. Relative standard deviations were less than 10% throughout all the recovery tests. In the residue analysis, agriculturally incurred pesticides at 0.08-0.14 mg/kg were designated with UV spectra compared with respective reference standards.     

Gas chromatographic determination of fenpropimorph residues in citrus fruit

A rapid gas chromatographic method for determining fenpropimorph residues in citrus fruit is reported. The fungicide is extracted with hexane after pH adjustment of the fruit homogenate. A short liquid-liquid partitioning process is performed before gas chromatography on an OV-17 column with nitrogen-phosphorus specific detection. The limit of detection of the method was 0.01 mg/kg, based on a 25 g sample. Recovery was always higher than 70%. Fenpropimorph residues in "Washington Navel" oranges and "Hernandina" clementine fruits dipped in a 1500 mg/L fungicide solution were determined. The fungicide remains mainly in the peel, with levels less than 0.1 mg/kg in the pulp. Fungicide residues in the peel decrease during storage, mainly in Washington Navel peel, where values decreased from 5.2 to 2.8 mg/kg.     

Plutonium in Gemüse,Obst und Böden

Plutonium in vegetables, fruits, and soils The plutonium content of fruits and vegetables from the Karlsruhe area in the years of 1971 - 1975 was determined. In vegetables the Pu-239 and Pu-240 activities were highly different, fluctuating between 64 fCi/kg fresh weight and values below the detection limit of about 6 fCi/kg. The activity of most of the vegetables and of all fruits was not higher than 10 fCi/kg. The nuclide Pu-238 could be identified in a qualitative way only, i. e. all measuring values remained below the detection limit of about 17 fCi/kg. The total-alpha-activities which were simultaneously determined in the vegetable and fruit ashes were in the range of 0.5 -19 pCi/kg fresh weight. Soil samples taken from the same region had a mean Pu-239 and Pu-240 activity of 5.9 ± 1.4 fCi/g and a total-alpha-activity of (10 ± 1.5) · 10³ fCi/g. Samples taken from the immediate vicinity of the Karlsruhe Nuclear Research Centre did not show increased activity levels.     

脲醛氮肥不影响叶菜的甲醛残留量及食用安全

【目的】研究脲醛氮肥(UF)对生菜、 上海青体内甲醛累积量、 甲醛含量的影响,评价其在叶菜上施用的安全性。【方法】采用3因素4水平完全设计分别进行第一次生菜及上海青盆栽试验。三因素为 50%尿素+50%脲醛[活性系数 (AI)=67%,UF1]、 50%尿素+50%脲醛(AI=57%,UF2)、 100%尿素(U); 施氮量设4个水平,分别是每千克土壤 N0、 100、 200、 300 mg,共 12 个处理,每个处理6个重复。采用4因素3水平完全设计进行第二次生菜盆栽试验,四个因素分别是AI分别为55%的脲醛(UF3)、 63%的脲醛(UF4)、 72%的脲醛(UF5)和100%尿素(U)(含 N 46%); 施氮量设3个水平,分别是每千克土壤 N 0、 200、 300 mg,共 12 个处理,每个处理6个重复。收获后,用乙酰丙酮法测定植株甲醛含量。【结果】无论施氮与否,生菜和上海青体内均能检测到甲醛。无氮对照在第一次生菜和上海青试验中的甲醛累积量分别为 0.21和0.35 mg/pot,在第二次生菜试验的生菜甲醛含量为 1.72 mg/kg。第一次生菜试验施 N 100 mg/kg土 时,U和 U+UF处理的生菜体内甲醛是无氮对照的 2.7~2.8 倍,但施氮处理间(U、 U+UF)无差异。在施 N 200、 300 mg/kg土时,U处理的生菜甲醛累积量明显大于U+UF处理,尿素处理甲醛累积量分别为 0.68、 0.56 mg/pot; 施 N 200 mg/kg土时,U处理的甲醛累积量比 UF1 + U和 UF2 + U 处理分别高出 26%、 33%,N 300 mg/kg土时的相应值是 30%、 24%。第二次生菜试验,两个施氮水平下,U和 UF5处理的甲醛含量最大; 施 N 200 mg/kg土时,UF3和UF4处理的生菜体内甲醛含量与无氮处理的无差异,当施氮量增加到300 mg/kg土时,活性指数为 55%的 UF3 与无氮处理的生菜甲醛含量无差异,活性指数为 63%的UF4的生菜甲醛含量甚至明显低于无氮处理。U和 UF5 处理的生菜甲醛含量范围为 4.97~5.36 mg/kg,UF3和 UF4处理的分别比 UF5处理的降低了68%和 70%,分别比U处理的的降低了69%和 71%。施氮量从 N 100 mg/kg土增加到 300 mg/kg土,均未影响叶菜体内的甲醛残留。【结论】与常规尿素相比,单独施用脲醛氮肥或尿素与脲醛氮肥混合施用,无论脲醛氮肥活性指数从 50%增加到 71%,还是氮肥用量从 100 增加到 300 mg/kg土,均不会增加叶菜体内的甲醛残留量,并且甲醛残留量处于安全范围内。     

Determination of bisphenol A in canned foods by immunoaffinity chromatography, HPLC, and fluorescence detection

Bisphenol A (BPA) concentrations were determined in canned beverages, fruits, vegetables, and fat-containing foodstuffs bought in Austrian supermarkets. The analysis method consisted of sol-gel immunoaffinity chromatography followed by high-performance liquid chromatography with fluorescence detection. With one exception traces of BPA were detected in all samples. BPA recovery strongly depended on the food matrix, ranging from 27% in goulash to 103% in a lemon soft drink. The results obtained allow a more realistic picture of the BPA exposure caused by cans with an epoxy resin protective coating because--in contrast to several previous studies--only those fractions of the can contents that are actually consumed were analyzed. BPA concentrations ranging from 0.1 ng/mL (lemon soft drink) to 38 ng/g (ready-to eat soup from Thailand) were significantly lower than the European Union migration limit of 0.6 mg of BPA/kg of food.     

改进型环形射流泵输送不同果蔬试验

为了研究果蔬的形状、大小和密度对环形射流泵输送性能的影响,该文采用喉管直径为60 mm的环形射流泵进行了马铃薯、胡萝卜、圣女果和金桔的输送试验,对入口结构、流量比、输送高度和果蔬种类4个因素对输送性能的影响进行了研究,并分析了不同工况对果蔬损伤的影响。结果表明:吸入口结构对输送性能有很大的影响;该环形射流泵输送马铃薯、胡萝卜、圣女果和金桔的最高输送能力分别为3 038.13、2 158.83、2 302.92和1 949.50 kg/h;在相同的面积比和输送高度下,输送能力随工作流体流量的增加而增大;在相同的面积比和工作流体流量下,输送能力随输送高度的升高而降低;在相同的工况下,输送椭球形的果蔬的能力要优于输送长条形果蔬的能力,输送密度较大的果蔬的能力要优于输送密度较小的果蔬的能力。输送单位质量果蔬的能耗在低流量比时变化不明显,在高流量比时有较大提升。马铃薯和胡萝卜在输送过程中损伤很小,损伤原因是与管道或泵内壁或者管道接口处碰撞,马铃薯和胡萝卜的最大质量损失率分别为0.13%和0.21%,最大表面损失率为0.68%和1.20%,圣女果和金桔在所有工况没有损伤。该研究可为今后环形射流泵输送果蔬的深入研究提供参考。     

High-amylose corn exhibits better antioxidant activity than typical and waxy genotypes

The consumption of fruits, vegetables, and whole grains rich in antioxidative phytochemicals is associated with a reduced risk of chronic diseases such as cancer, coronary heart disease, diabetes, Alzheimer's disease, cataract, and aged-related functional decline. For example, phenolic acids are among the main antioxidative phytochemicals in grains that have been shown to be beneficial to human health. Corn (Zea mays L.) is a major staple food in several parts of the world; thus, the antioxidant activity of several corn types was evaluated. The 2,2-Diphenyl-1-picryhydrazyl free radical (DPPH*) scavenging activity, total phenolic content (TPC), antioxidant capacity of lipid-soluble substances (ACL), oxygen radical absorbance capacity (ORAC), and phenolic acid compositions of typical and mutant genotypes (typical-1, waxy, typical-2, and high-amylose) were investigated. The DPPH* scavenging activity at 60 min was 34.39-44.51% in methanol extracts and 60.41-67.26% in HCl/methanol (1/99, v/v) extracts of corn. The DPPH* scavenging activity of alkaline hydrolysates of corn ranged from 48.63 to 64.85%. The TPC ranged from 0.67 to 1.02 g and from 0.91 to 2.15 g of ferulic acid equiv/kg of corn in methanol and HCl/methanol extracts, respectively. The TPC of alkaline hydrolysates ranged from 2.74 to 6.27 g of ferulic acid equiv/kg of corn. The ACL values were 0.41-0.80 and 0.84-1.59 g of Trolox equiv/kg of corn in methanol and HCl/methanol extracts, respectively. The ORAC values were 10.57-12.47 and 18.76-24.92 g of Trolox equiv/kg of corn in methanol and HCl/methanol extracts, respectively. ORAC values of alkaline hydrolysates ranged from 42.85 to 68.31 g of Trolox equiv/kg of corn. The composition of phenolic acids in alkaline hydrolysates of corn was p-hydroxybenzoic acid (5.08-10.6 mg/kg), vanillic acid (3.25-14.71 mg/kg), caffeic acid (2.32-25.73 mg/kg), syringic acid (12.37-24.48 mg/kg), p-coumaric acid (97.87-211.03 mg/kg), ferulic acid (1552.48-2969.10 mg/kg), and o-coumaric acid (126.53-575.87 mg/kg). Levels of DPPH* scavenging activity, TPC, ACL, and ORAC in HCl/methanol extracts were obviously higher than those present in methanol extracts. There was no significant loss of antioxidant capacity when corn was dried at relatively high temperatures (65 and 93 degrees C) postharvest as compared to drying at ambient temperatures (27 degrees C). Alkaline hydrolysates showed very high TPC, ACL, and ORAC values when compared to methanol and HCl/methanol extracts. High-amylose corn had a better antioxidant capacity than did typical (nonmutant) corn genotypes.     

Content of the flavonols quercetin, myricetin, and kaempferol in 25 edible berries

The amounts of quercetin, myricetin, and kaempferol aglycons in 25 edible berries were analyzed by an optimized RP-HPLC method with UV detection and identified with diode array and electrospray ionization mass spectrometry detection. Sixteen species of cultivated berries and nine species of wild berries were collected in Finland in 1997. Quercetin was found in all berries, the contents being highest in bog whortleberry (158 mg/kg, fresh weight), lingonberry (74 and 146 mg/kg), cranberry (83 and 121 mg/kg), chokeberry (89 mg/kg), sweet rowan (85 mg/kg), rowanberry (63 mg/kg), sea buckthorn berry (62 mg/kg), and crowberry (53 and 56 mg/kg). Amounts between 14 and 142 mg/kg of myricetin were detected in cranberry, black currant, crowberry, bog whortleberry, blueberries, and bilberry. Kaempferol was detected only in gooseberries (16 and 19 mg/kg) and strawberries (5 and 8 mg/kg). Total contents of these flavonols (100-263 mg/kg) in cranberry, bog whortleberry, lingonberry, black currant, and crowberry were higher than those in the commonly consumed fruits or vegetables, except for onion, kale, and broccoli.     

小叶女贞果实花青素组分鉴定及色谱纯化技术

为提高小叶女贞果实的食用、药用价值,该文系统研究了果实中花青素种类构成及提取物的制备技术。试验采用紫外可见光谱法、高效液相色谱-质谱串联法、酸水解制备苷元等技术对小叶女贞果实花青素含量、单体种类进行了测定,并借助提取、萃取、柱层析等技术研究了花青素提取物的分离纯化过程。研究结果如下:测得每100 g小叶女贞果实中含花青素总量为(499±18.42)mg,从中鉴定出2种花青素单体,分别为矢车菊素-3-O-葡萄糖苷和牵牛花色素-3-O-葡萄糖苷,并以后者为主要存在形式;获得了纯天然、简单易行的花青素提取物制备技术,主要包括酸化乙醇提取、乙酸乙脂萃取、Amberlite XAD-7HP大孔树脂层析分离步骤,最终制得的花青素提取物纯度为35%、得率为0.6%。该研究为后期制备高纯度牵牛花素-3-O-葡萄糖苷单体提供了良好原料基础,为深入研究小叶女贞果实花青素功能活性及其在食品、药品领域潜在应用提供了参考。     

Associations between parental report of the home food environment and adolescent intakes of fruits, vegetables and dairy foods

OBJECTIVE: This study examines parental report of household food availability, parent dietary intake and associations with adolescent intakes of fruits, vegetables and dairy foods. DESIGN: Cross-sectional study. Adolescents completed the Project EAT survey and the Youth Adolescent Food Frequency Questionnaire at school. Parents of adolescents were interviewed by telephone about the home food environment, eating habits and weight-related behaviours. General linear modelling was used to compare dietary intakes of adolescents across different levels of household food availability and parental intakes. SUBJECTS/SETTING: The study sample included 902 adolescents and their parent or guardian. RESULTS: Many parents were not consuming the minimum number of daily recommended fruit (44.5%), vegetable (69.9%) or dairy (46.9%) servings. While most parents reported that fruits and vegetables were available at home (90.3%) and vegetables were usually served at dinner (87.0%), fewer parents reported milk was served at meals (66.6%). Soft drinks were usually available at home (56.8%). Among girls, household availability was positively associated with fruit and vegetable intake (ttrend=2.70, P<0.01) and soft drink availability was inversely associated with dairy intake (ttrend=2.08, P=0.04). Among boys, serving milk at meals was positively associated with dairy intake (ttrend=3.65, P<0.01). Parental intakes were positively associated with dairy intake for boys (ttrend=2.04, P=0.04), and with dairy (ttrend=2.43, P=0.01), vegetable (ttrend=3.72, P<0.01) and fruit (ttrend=3.17, P<0.01) intakes for girls. CONCLUSIONS/APPLICATIONS: Interventions designed to help adolescents improve consumption of fruits, vegetables and dairy foods may be enhanced by including a parental component aimed at increasing household availability and parents' intake of healthful food choices.     

Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity

Processed fruits and vegetables have been long considered to have lower nutritional value than their fresh commodities due to the loss of vitamin C during processing. This research group found vitamin C in apples contributed < 0.4% of total antioxidant activity, indicating most of the activity comes from the natural combination of phytochemicals. This suggests that processed fruits and vegetables may retain their antioxidant activity despite the loss of vitamin C. Here it is shown that thermal processing elevated total antioxidant activity and bioaccessible lycopene content in tomatoes and produced no significant changes in the total phenolics and total flavonoids content, although loss of vitamin C was observed. The raw tomato had 0.76 +/- 0.03 micromol of vitamin C/g of tomato. After 2, 15, and 30 min of heating at 88 degrees C, the vitamin C content significantly dropped to 0.68 +/- 0.02, 0.64 +/- 0.01, and 0.54 +/- 0.02 micromol of vitamin C/g of tomato, respectively (p < 0.01). The raw tomato had 2.01 +/- 0.04 mg of trans-lycopene/g of tomato. After 2, 15, and 30 min of heating at 88 degrees C, the trans-lycopene content had increased to 3.11+/- 0.04, 5.45 +/- 0.02, and 5.32 +/- 0.05 mg of trans-lycopene/g of tomato (p < 0.01). The antioxidant activity of raw tomatoes was 4.13 +/- 0.36 micromol of vitamin C equiv/g of tomato. With heat treatment at 88 degrees C for 2, 15, and 30 min, the total antioxidant activity significantly increased to 5.29 +/- 0.26, 5.53 +/- 0.24, and 6.70 +/- 0.25 micromol of vitamin C equiv/g of tomato, respectively (p < 0.01). There were no significant changes in either total phenolics or total flavonoids. These findings indicate thermal processing enhanced the nutritional value of tomatoes by increasing the bioaccessible lycopene content and total antioxidant activity and are against the notion that processed fruits and vegetables have lower nutritional value than fresh produce. This information may have a significant impact on consumers' food selection by increasing their consumption of fruits and vegetables to reduce the risks of chronic diseases.     

Association between consumption of cruciferous vegetables and condiments and excretion in urine of isothiocyanate mercapturic acids

A high intake of cruciferous vegetables is associated with a reduced risk of cancer and cardiovascular diseases. This protective effect has been linked to isothiocyanates, enzymatic hydrolysis products of glucosinolates. In this study, the metabolic fate of glucosinolates and isothiocyanates after ingestion of 19 different cruciferous vegetables was studied in three male subjects. After the consumption of 13 cruciferous vegetables (glucosinolate content, 0.01-0.94 mmol/kg) and six condiments (isothiocyanate content, 0.06-49.3 mmol/kg), eight different isothiocyanate mercapturic acids were determined in urine samples. Excretion levels after the consumption of raw vegetables and condiments were higher (bioavailability, 8.2-113%) as compared to cooked vegetables (bioavailability, 1.8-43%), but the excretion rate was similar (t1/2=2.1-3.9 h). Isothiocyanates in urine remain longer at a nonzero level after the consumption of glucosinolates from cooked vegetables, as compared to raw vegetables and condiments, and maximal levels in urine were reached about 4 h later. Isothiocyanate mercapturic acids can be used as a biomarker to reflect the active dose of isothiocyanates absorbed.     

Determination of ascorbic acid and carotenoids in food commodities by liquid chromatography with mass spectrometry detection

Two methods, one to determine ascorbic acid and one to determine lycopene and beta-carotene, in vegetables and fruits by liquid chromatography coupled with mass spectrometry (LC-MS) have been established. The chromatographic separation of the studied compounds and their MS parameters were optimized to improve selectivity and sensitivity. In both methods, separation was carried out with two coupled columns, first a C(18) and then a dC(18), using as mobile phase 70% methanol (0.005% acetic acid) and 30% acetic acid 0.05% for ascorbic acid determination and a mixture of methanol, tetrahydrofuran, and acetonitrile (60:30:10 v/v/v) for carotenoid analysis in isocratic mode. The molecular ion was selected for the quantification in selective ion monitoring (SIM) mode. Ascorbic acid was detected with electrospray ionization probe (ESI) in negative mode, while chemical ionization atmospheric pressure (APCI) in positive mode was used for the target carotenoids. The methodology for ascorbic acid analysis is based on an extraction with polytron using methanol and a mixture of methaphosphoric acid and acetic acid. Extraction of the carotenoids was carried out with tetrahydrofuran/methanol (1:1) (v/v). The proposed methods were applied, after their corresponding validations, to the analysis of four varieties of tomatoes, tomato in tin enriched and dried tomato, and to the analysis of mango and kiwi fruits, to compare the content in these compounds. Moreover, the influence of the process of freezing and the effect that the manipulation/preservation has in the content of ascorbic acid in tomato have also been studied.