不同鸡新城疫疫苗免疫鸡血清HI抗体的测定

将试验鸡分成3个试验组和1个对照组。A组鸡接种鸡新城疫系疫苗,B组鸡接种油乳剂灭活苗,C组鸡接种鸡新城疫系疫苗,并在接种疫苗后第3、4、5、6、7、9、11、13、15、20、25d采取各组鸡血并分离血清,检测HI抗体。结果表明,接种系疫苗的组,HI抗体效价均值从4.67log2上升到10log2,接种后第5d开始上升,接种后第11d达到峰值,持续6d保持高滴度抗体水平。接种系疫苗的组,HI抗体效价均值从4.67log2上升到7log2,接种后第4d开始上升,接种后第9d达到峰值。接种油乳剂灭活苗的组,HI抗体效价均值从4.67log2上升到9.33log2,接种后第5d开始上升,接种后第11d达到峰值,持续16d保持高滴度抗体水平。系疫苗HI抗体效价上升快,效价高,较适合于紧急接种,油乳剂灭活苗HI抗体效价可在高水平维持较长时间,较适合于预防接种。     

重组禽流感灭活苗和禽流感灭活苗对鸡免疫效果的观察

用重组禽流感灭活苗接种10日龄、14日龄和21日龄的SPF鸡,接种后HI抗体效价无显著差异。将H5N1和H5N2疫苗分别接种21日龄SPF鸡,结果表明,H5N1和H5N2均能刺激SPF鸡产生较高的HI抗体;分别接种三黄鸡,接种后21 d,H5N1能刺激三黄鸡产生较高的HI抗体;而H5N2不能刺激三黄鸡产生合格的HI抗体,与SPF鸡免疫组相比差异显著。经过二次接种,HI抗体平均为8.9 log2,与SPF鸡组接种后42 d的各组相比差异不显著,而与一免后21 d的各组HI抗体效价相比差异显著。表明应用禽流感灭活苗对三黄鸡免疫接种,必须进行二免方可达到理想免疫效果,而应用重组禽流感灭活苗对三黄鸡进行免疫接种,一次免疫即可获得较高的HI抗体效价。     

鸡新城疫诊断抗原制备方法的研究

将疑似新城疫病料无菌采集后进行RT-PCR鉴定,将阳性病料研磨后接种SPF鸡胚,收获的阳性尿囊液进行血凝试验(HA)及血凝抑制试验(HI)检测病毒效价,病毒效价达到2~7~2~8,病毒凝集效价达到10log2。使用10%蔗糖溶液及18-25k Da透析袋对阳性尿囊液进行浓缩,浓缩至原体积的1/5;10%福尔马林灭活诊断抗原,经过6h后灭活达100%;诊断抗原效价测定:血凝效价为2~(10);血凝抑制效价为10log2。利用制备的鸡新城疫诊断抗原和鸡新城疫标准诊断抗原与鸡新城疫标准检测抗体进行血凝试验(HA)与血凝抑制试验(HI),对比结果:鸡新城疫诊断抗原病毒效价为2~(10),高于鸡新城疫标准诊断抗原的2~9,鸡新城疫诊断抗原凝集效价为10log2,等同于鸡新城疫标准诊断抗原,证明所制备鸡新城疫诊断抗原符合要求,可以应用到生产实践中。     

合肥野生动物园5种雉鸡接种新城疫疫苗后抗体水平测定

选择成年白冠长尾雉5只,白腹锦鸡5只,白鹇3只,红腹锦鸡3只和孔雀11只,同时用L系弱毒冻干苗和新城疫油乳剂灭活疫苗进行免疫接种,通过血凝抑制试验(HI)测定其抗体滴度.结果表明,在免疫接种后26 d,白冠长尾雉抗体效价为9.8log2,白腹锦鸡为8.0log2,白鹇为8.0log2,红腹锦鸡为10.7log2;孔雀在免疫后接种0、8、15、20、25 d,抗体水平分别为3.8log2、5.0log2、6.2log2、7.5log2及8.2log2.     

鸡传染性支气管炎病毒M41株和Conn株的血清交叉血凝抑制试验

本试验用系统血凝抑制(HI)交叉试验,对鸡传染性支气管炎病毒M41株和Conn株进行抗体区分。结果表明,M41株和Conn株标准抗原与M41阳性血清HI抗体效价在免疫后21 d分别为8 log2、5 log2,相差3 log2;免疫后28 d分别为8 log2、5 log2,相差3 log2;免疫后35 d分别为8 log2、5 log2,相差3 log2。M41株和Conn株标准抗原与Conn阳性血清HI抗体效价在免疫后21 d分别为4 log2、8 log2,相差4;免疫后28 d分别为4 log2、8 log2,相差4 log2;免疫后35 d分别为4 log2、9 log2,相差5 log2。因此,用血清HI交叉试验能够将抗M41株和Conn株抗体区分开来。     

兔出血症-巴氏杆菌病二联蜂胶灭活苗的研制及免疫效果的观察

采用对本地区具有良好免疫原性的兔病毒性出血症强毒株和巴氏杆菌菌株,研制出常规二联灭活苗和蜂胶二联灭活苗.用血清学方法对常规二联灭活苗和蜂胶二联灭活苗免疫后兔病毒性出血症抗体的检测.结果表明:常规二联灭活苗免疫后7 d HI抗体可达5.0log2,15 d明显上升,达5.510g2,30 d达到高峰,可达8.0log2,;而蜂胶二联灭活苗在免疫后7 d HI抗体可达5.0log,15d明显上升,达5.5log2以上,20 d显著上升9.25log2以上,30 d达到高峰,可达11.0log2.说明蜂胶提高抗体效价作用明显,可显著增强免疫效果.     

甘肃省肉仔鸡HPAIH5亚型母源抗体消长规律研究

用HI法对甘肃省肉仔鸡H5N1型禽流感（HPAI）母源抗体消长规律进行动态监测,结果显示,肉仔鸡母源抗体保护期长达67 d。其中,种蛋的母源抗体效价最高为9.48 log2,1日龄肉仔鸡母源抗体效价均值为7.95 log2,4 d降为7.81 log2,7 d母源抗体升至高峰,达8.57log2,接近父母代抗体效价8.80 log2;随后,抗体效价逐渐下降并略有波动;67-70 d,抗体效价迅速降至1.67 log2,79 d趋于消失,为1.83 log2。该测定结果提示,HI试验在HPAI诊断中意义不大,仅能作为参考,确诊需进行病毒的分离培养。     

H5Rel与H5N28两种禽流感疫苗对肉番鸭免疫效果对比试验

用H5Rel与H5N28两种疫苗在肉番鸭体内的免疫效果对比研究。首免后35d,两组抗体效价没有明显差异。二免后19d,H5Rel组抗体效价提高0.9log2,至免后25d效价达到最高峰值8.4log2,比H5N28组效价高2.9log2。以后相对稳定,至二免后60d抗体效价H5Rel组平均维持在7.3log2,H5N28组平均为5log2。     

H5Rel与H5N28两种禽流感疫苗对肉番鸭免疫效果对此试验

用H5Rel与H5N28两种疫苗在肉番鸭体内的免疫效果对比研究.首免后35d,两组抗体效价没有明显差异.二免后19d,H5Rel组抗体效价提高0.9 log2,至免后25d效价达到最高峰值8.4 log2,比H5N28组效价高2.9 log2.以后相对稳定,至二免后60d抗体效价H5Rel组平均维持在7.3 log2,H5N28组平均为5 log2.     

H5Rel与H5N28两种禽流感疫苗对肉番鸭免疫效果的对比试验

用H5Rel与H5N28两种疫苗在肉番鸭体内的免疫效果对比研究。首免后35d，两组抗体效价没有明显差异。二免后19d，H5Rel组抗体效价提高0．9log2，至免后25d效价达到最高峰值8．4log2，比H5N28组效价高2．9log2。以后相对稳定，至二免后60d抗体效价H5Rel组平均维持在7.3log2，H5N28组平均为5log2。     

2009-2010年新疆地区活禽市场肉鸡禽流感H5抗体监测与分析

为了解新疆地区活禽市场中肉鸡禽流感H5抗体的情况,对2009-2010年新疆五个地区八个大型活禽批发市场采集的37批次1225份血清样品进行了AIH5抗体监测。结果表明,AIH5抗体效价≥4.0log2样品占总样品率的32.5%,0效价样品率达到54.5%;不同地区和不同市场的样品AIH5抗体效价存在较大差异;在37批次样品中有9批次样品来自于免疫鸡群,抗体效价≥4.0log2样品率达到99.2%以上;28批次样品来自于非免疫鸡群,0效价样品率高达71.3%。     

ELISA检测DLV虫苗免疫鸡抗体消长规律的研究

将柔嫩、毒害、巨型艾美耳球虫的孢子化卵囊制成可溶性抗原，用酶联免疫吸附试验检测鸡球虫双重致弱多凤苗虫苗、为柔嫩、毒害、巨型艾美耳球虫三价苗免疫鸡血清抗球虫抗体的消长规律。     

不同新城疫弱毒疫苗在紧急免疫中临床应用效果比较

对发生新城疫感染的某蛋鸡养殖场,将发病鸡群分成A组、B组、C组,并分别用ZM10株、Clone30株、LaSota株三种不同新城疫弱毒株疫苗进行紧急免疫,监测并记录每组蛋鸡死亡率、产蛋率和抗体效价变化情况,并进行比较分析。结果表明,用ZM10株弱毒疫苗进行紧急免疫要优于LaSota株和Clone30株。     

鸡传染性法氏囊病免疫复合物疫苗对1日龄低母源抗体水平雏鸡免疫效果的研究

将鸡传染性法代囊病病毒(IBDV)BX株抗原和IBDV高免血清按一定比例混合,初步制备了无菌的鸡传染性法氏囊病免疫复合物疫苗1~5(分别含有32、8、4、0.5及0.125单位IBDV中和抗体),用鸡传染性法氏囊病免疫复合物疫苗1~5及BX株活疫苗分别免疫1日龄低母源抗体水平雏鸡,免疫后9 d观察法氏囊病变,免疫后28 d采血测定IBDV中和抗体,并用强毒攻击,计算各组疫苗保护率。结果显示,免疫后9 d,复合物疫苗1~3免疫组鸡法氏囊正常,复合物疫苗4、5免疫组和BX株活疫苗免疫组分别有2/10、4/10和5/10的试验鸡法氏囊出现了病变;免疫后28 d,复合物疫苗1~5免疫组和BX株活疫苗免疫组IBDV中和抗体效价分别为8.34log2、9.60log2、9.21log2、7.88log2、9.50log2和9.12log2,攻毒保护率分别为90%、100%、100%、80%、90%和80%。试验结果表明鸡传染性法氏囊病免疫复合物疫苗2、3(分别含8、4单位IBDV中和抗体)免疫1日龄低母源抗体水平的雏鸡效果最好,攻毒保护率能达到100%。     

Study on the Immunity Effect of Immune Complex Vaccine for Infectious Bursal Disease on One-day Old Low Maternal Antibody Chicken

Five kinds of infectious bursal disease (IBD) immune complex (IC) vaccines were prepared with infectious bursal disease virus (IBDV) BX strain mixed with IBDV hyperimmune serum according to a certain proportion (containing 32, 8, 4, 0.5 and 0.125 units IBDV neutralizing antibody, respectively).One-day old low maternal antibody chickens were vaccinated with IBD IC vaccines 1 to 5 and BX strain live vaccine, respectively, pathological changes of the bursa of fabricius in chickens were observed at the 9th day after immunization.On the day of 28 after immunization, blood samples were taken and the IBDV neutralizing antibody were tested, meanwhile all experimental chickens were challenged with high virulent IBDV, the protective rates of vaccines were calculated.The results showed that at the 9th day after immunization, the bursa of fabricius were normal in IC vaccine 1, 2 and 3 groups, however 2/10, 4/10 and 5/10 of pathological changes of the bursa of fabricius in IC vaccine 4, 5 groups and BX strain vaccine group, respectively.At the 28th day after immunization, the IBDV neutralizing antibodies in IC vaccine 1, 2, 3, 4, 5 groups and BX strain vaccine group were 8.34log2, 9.60log2, 9.21log2, 7.88log2, 9.50log2 and 9.12log2, while 90%, 100%, 100%, 80%, 90% and 80% protection rates were provided, respectively.The results showed that IBD IC vaccines 2 and 3 (containing 8, 4 units IBDV neutralizing antibody, respectively) had the best immunity effect on one-day old low maternal antibody chickens, protection rates were both 100%.     

重组禽流感病毒灭活疫苗(H5N1亚型,Re-1株)免疫鸡抗体消长规律及免疫程序的初步研究

研究新型重组H5N1亚型禽流感灭活疫苗对种鸡和肉鸡的免疫原性,并对雏鸡母源抗体和免疫后的抗体进行动态观察,根据试验结果推荐该疫苗对鸡的免疫程序。用HI方法检测种鸡、肉鸡的母源抗体和免疫抗体,根据母源抗体的衰减和免疫抗体的消长规律确定首免和再免日龄。结果表明种鸡的母源抗体约能维持10 d多;0.3 mL/羽首免后10 d HI抗体就可达到6.40 log2,3-5周达到高峰期,至免疫17周后(19周龄)HI抗体水平仍然维持在4.88 log2;19周龄时0.5 mL/羽进行二免,有效抗体能维持约20周;280日龄0.5 mL/羽三免后抗体水平均一,下降缓慢,至种鸡淘汰时(三免后29周)抗体水平仍能维持在5.32 log2。肉鸡母源抗体约能维持7d,10日龄时0.3 mL/羽免疫,有效抗体能维持到上市。新型重组H5N1亚型禽流感灭活疫苗对鸡的免疫原性确实。     

鸡传染性法氏囊病免疫复合物疫苗对1日龄中等母源抗体水平雏鸡免疫效果的研究

将鸡传染性法氏囊病免疫复合物疫苗和BX株活疫苗分别免疫1日龄中等母源抗体水平京红雏鸡,同时免疫1日龄SPF雏鸡作对照,免疫后第9天观察法氏囊病变,免疫后第28天采血测定IBDV中和抗体,并用强毒攻击,计算各组疫苗保护率。结果显示,免疫后第9天,免疫复合物疫苗组SPF雏鸡和京红雏鸡法氏囊均正常,BX株活疫苗组SPF雏鸡法氏囊出现了萎缩病变;免疫后第28天,对照SPF雏鸡和京红雏鸡的免疫复合物疫苗组、BX活疫苗组IBDV中和抗体效价分别为9.2log2、8.5log2和 7.2log2、4.4log2,攻毒保护率均为100%。试验结果表明免疫复合物疫苗免疫1日龄中等水平母源抗体雏鸡效果很好,能产生较高的中和抗体,攻毒保护率能达到100%。     

Study on the Immunity Effect of Infectious Bursal Disease Immune Complex Vaccine on One-day Old Moderate Maternal Antibody Chick

One-day-old moderate maternal antibody Jinghong chickens and SPF chickens were vaccinated with infectious bursal disease (IBD) immune complex (IC) vaccine and BX strain live vaccine,respectively,pathological changes of bursa of fabricius in chickens were observed at the 9th day after immunization.On the 28th day after immunization,blood samples were taken and the IBDV neutralizing antibody were tested,meanwhile experimental chickens were challenged with high virulent IBDV,the protective rates of experimental groups were calculated.The results showed that at the 9th day after immunization,the bursa of fabricius in SPF and Jinghong chickens were normal in IC vaccine group,however atrophic changes of bursa of fabricius in BX strain live vaccine group were shown.At the 28th day after immunization,the IBDV neutralizing antibody of IC vaccine group and BX strain live vaccine group in SPF and Jinghong chickens were 9.2log2,8.5log2,7.2log2 and 4.4log2,respectively,while 100% protection rates were provided.The results provided that IC vaccine had better immunity effect on one-day-old moderate maternal antibody chicken,high levels of neutralizing antibodies against IBDV were produced and protection rates were 100%.     

豆类丝核菌次级代谢产物对鸡免疫功能的影响

研究豆类丝核菌次级代谢产物对鸡免疫功能的影响.试验一将200只14日龄健康未免疫雏公鸡随机分为8组,Ⅰ-Ⅳ组动物于14日龄以新城疫活疫苗滴鼻进行第1次免疫,2周后加强免疫1次,V-Ⅷ组不免疫.Ⅰ-Ⅲ组、Ⅴ-Ⅶ组为试验组,分别注射高、中、低(1、2、4 mg/kg)3种剂量的豆类丝核菌次级代谢产物.Ⅳ组和Ⅷ组分别为疫苗对...