Effects of activated charcoal and tannin added to compost and to soil on carbon dioxide,nitrous oxide and ammonia volatilization

Given high mineralization rates of soil organic matter addition of organic fertilizers such as compost and manure is a particularly important component of soil fertility management under irrigated subtropical conditions as in Oman. However, such applications are often accompanied by high leaching and volatilization losses of N. Two experiments were therefore conducted to quantify the effects of additions of activated charcoal and tannin either to compost in the field or directly to the soil. In the compost experiment, activated charcoal and tannins were added to compost made from goat manure and plant material at a rate of either 0.5 t activated charcoal ha^?1, 0.8 t tannin extract ha^?1, or 0.6 t activated charcoal and tannin ha^?1 in a mixed application. Subsequently, emissions of CO₂, N₂O, and NH₃ volatilization were determined for 69 d of composting. The results were verified in a 20‐d soil incubation experiment in which C and N emissions from a soil amended with goat manure (equivalent to 135 kg N ha^?1) and additional amendments of either 3 t activated charcoal ha^?1, or 2 t tannin extract ha^?1, or the sum of both additives were determined. While activated charcoal failed to affect the measured parameters, both experiments showed that peaks of gaseous CO₂ and N emission were reduced and/or occurred at different times when tannin was applied to compost and soil. Application of tannins to compost reduced cumulative gaseous C emissions by 40% and of N by 36% compared with the non‐amended compost. Tannins applied directly to the soil reduced emission of N₂O by 17% and volatilization of NH₃ by 51% compared to the control. However, emissions of all gases increased in compost amended with activated charcoal, and the organic C concentration of the activated charcoal amended soil increased significantly compared to the control. Based on these results, tannins appear to be a promising amendment to reduce gaseous emissions from composts, particularly under subtropical conditions.     

有机无机肥配施对酸性菜地土壤硝化作用的影响

通过室内培养和田间试验, 研究了有机无机肥配施对酸性菜地土硝化作用的影响。培养试验条件为60%土壤最大持水量和25 ℃。 结果表明,土壤硝化作用模式为指数方程,延滞期10天。与纯化肥处理（NPK）相比,鲜猪粪配施无机肥（FPM+NPK）和猪粪堆肥配施无机肥（CPM+NPK）均能降低土壤硝化势和氨氧化潜势,猪粪堆肥配施无机肥还能增加土壤微生物量碳、 氮。鲜猪粪配施无机肥和猪粪堆肥配施无机肥处理在硝化培养和田间试验期间N2O释放量均没有差异,但硝化培养期间鲜猪粪配施无机肥的N2O释放量显著低于纯化肥处理,田间试验期间猪粪堆肥配施无机肥的N2O释放量显著低于纯化肥处理。培养试验结束后的土壤pH值与土壤硝化势间,以及硝化培养期间N2O累积释放量与土壤硝化势间均存在显著正相关关系。本研究表明, 有机无机肥配施显著影响土壤硝化作用以及硝化培养期间和田间N2O释放。     

Nitrous Oxide Emissions from a Coal Mine Land Reclaimed with Stabilized Manure

Mine‐soil treatment using stabilized manure rapidly sequesters large quantities of organic carbon and nutrients. However, the nutrient‐rich soil conditions may become highly conducive for production and emission of N₂O. We examined this possibility in a Pennsylvania coal mine restored using poultry manure stabilized in two forms: composted (Comp) or mixed with paper mill sludge (Man + PMS) at C/N ratios of 14, 21, and 28 and compared those with the emissions from conventionally treated soil. The mine soil was extremely well drained with 59% coarse fragments. Soil–atmosphere exchange of N₂O and CO₂ was determined using a sampling campaign of ten measurements between 16 June and 14 September 2009 (90 days) and 13 measurements between 28 June and 9 November 2010 (134 days) using static vented chambers at ambient and increased moisture (water added) content. Potential denitrification was determined in a laboratory incubation experiment. While non‐amended mine soil did not have a measurable potential for denitrifying activity, the manure‐based amendments introduced the potential. Soil water filled pore space was less than 60% on most sampling days in both ambient and water‐added plots. Daily N₂O‐N emissions ranged between 40 and 70 g N ha⁻¹ with cumulative emissions of 2–4 kg N ha⁻¹ from non‐amended, lime and fertilizer (L + F) and Comp, and 3–10 kg N ha⁻¹ from Man + PMS treatments. The maximum emission obtained from Man + PMS represented <1% loss of applied N. Although stabilized manure‐treated soil exhibits the potential for N₂O production, the emission is limited when soils are excessively well drained and reducing conditions rarely develop. Copyright © 2015 John Wiley & Sons, Ltd.     

Effect of the long-term application of organic matter on soil carbon accumulation and GHG emissions from a rice paddy field in a cool-temperate region,Japan-II. Effect of different compost applications-

ABSTRACT

The influence of long-term application of different types of compost on rice grain yield, CH₄ and N₂O emissions, and soil carbon storage (0 ? 30 cm) in rice paddy fields was clarified. Two sets of paddy fields applied with rice straw compost or livestock manure compost mainly derived from cattle were used in this study. Each set comprised long-term application (LT) and corresponding control (CT) plots. The application rates for rice straw compost (42 years) and livestock manure compost (41 years in total with different application rates) were 20 Mg fresh weight ha^–1. Soil carbon storage increased by 33% and 37% with long-term application of rice straw compost and livestock manure compost, respectively. The soil carbon sequestration rate by the organic matter application was 23% higher with the livestock manure compost than with the rice straw compost. The rice grain yield in the LT plot was significantly higher than that in the corresponding CT plot with both types of compost. Although the difference was not significant in the rice straw compost, cumulative CH₄ emissions increased with long-term application of both composts. Increase rate of CH₄ emission with long-term application was higher in the livestock manure compost (99%) than that in the rice straw compost (26%). In both composts, the long-term application did not increase N₂O emission significantly. As with the rice straw compost, the increase in CH₄ emission with the long-term application of livestock manure compost exceeded the soil carbon sequestration rate, and the change in the net greenhouse gas (GHG) balance calculated by the difference between them was positive, indicating a net increase in the GHG emissions. The increase in CH₄ and net GHG emissions owing to the long-term application of the livestock manure compost could be higher than that of the rice straw compost owing to the amount of applied carbon, the quality of compost and the soil carbon accumulation. The possibility that carbon sequestration in the subsoil differs depending on the type of composts suggests the importance of including subsoil in the evaluation of soil carbon sequestration by long-term application of organic matter.     

施肥方式对紫色土农田生态系统N2O和NO排放的影响

依托紫色土施肥方式与养分循环长期试验平台(2002年—),采用静态箱-气相色谱法开展紫色土冬小麦-夏玉米轮作周期(2013年10月至2014年10月)农田生态系统N_2O和NO排放的野外原位观测试验。长期施肥方式包括单施氮肥(N)、传统猪厩肥(OM)、常规氮磷钾肥(NPK)、猪厩肥配施氮磷钾肥(OMNPK)和秸秆还田配施氮磷钾肥(RSDNPK)等5种,氮肥用量相同[小麦季130 kg(N)×hm~(-2),玉米季150 kg(N)×hm~(-2)],不施肥对照(CK)用于计算排放系数,对比不同施肥方式对紫色土典型农田生态系统土壤N_2O和NO排放的影响,以期探寻紫色土农田生态系统N_2O和NO协同减排的施肥方式。结果表明,所有施肥方式下紫色土N_2O和NO排放速率波动幅度大,且均在施肥初期出现峰值;强降雨激发N_2O排放,但对NO排放无明显影响。在整个小麦-玉米轮作周期,N、OM、NPK、OMNPK和RSDNPK处理的N_2O年累积排放量分别为1.40 kg(N)×hm~(-2)、4.60 kg(N)×hm~(-2)、0.95 kg(N)×hm~(-2)、2.16kg(N)×hm~(-2)和1.41 kg(N)×hm~(-2),排放系数分别为0.41%、1.56%、0.25%、0.69%、0.42%;NO累积排放量分别为0.57 kg(N)×hm~(-2)、0.40 kg(N)×hm~(-2)、0.39 kg(N)×hm~(-2)、0.46 kg(N)×hm~(-2)和0.17 kg(N)×hm~(-2),排放系数分别为0.21%、0.15%、0.15%、0.17%、0.07%。施肥方式对紫色土N_2O和NO累积排放量具有显著影响(P0.05),与NPK处理比较,OM和OMNPK处理的N_2O排放分别增加384%和127%,同时NO排放分别增加3%和18%;RSDNPK处理的NO排放减少56%。表明长期施用猪厩肥显著增加N_2O和NO排放,而秸秆还田有效减少NO排放。研究表明,土壤温度和水分条件均显著影响小麦季N_2O和NO排放(P0.01),对玉米季N_2O和NO排放没有显著影响(P0.05),土壤无机氮含量则是在小麦-玉米轮作期N_2O和NO排放的主要限制因子(P0.01)。全量秸秆还田与化肥配合施用是紫色土农田生态系统N_2O和NO协同减排的优化施肥方式。     

Effects of green manure storage and incorporation methods on nitrogen release and N2O emissions after soil application

More efficient use of green manure-derived nitrogen (N) may improve crop yields and reduce environmental impacts in stockless organic arable farming. In this 3-month incubation study, we tested a new strategy where green manure leys are harvested and preserved until the following spring either as compost mixed with straw or as silage of harvested ley biomass. Grass-clover compost or silage was soil-incorporated by either simulated ploughing (green manure placed at 15 cm depth) or harrowing (green manure mixed into the upper 5-cm soil horizon) in order to assess treatment effects on net release of plant-available N, nitrous oxide (N₂O) fluxes and soil respiration. Grass-clover silage provided the highest net N release with similar results for the two incorporation methods. Up to one third of the total N content in silage became plant-available during the 3 months. In contrast, no net N release was observed for the composted grass-clover and straw mixture. In fact, soil incorporation of compost by harrowing caused temporal immobilization of soil mineral N. Silage incorporated by ploughing gave rise to the largest N₂O effluxes with silage-induced emissions corresponding to 0.3 % of applied total N. Possibly N₂O production via denitrification was stimulated by oxygen-limited conditions near the decomposing silage. In contrast, compost incorporated by harrowing caused net N₂O uptake, presumably an effect of reduced mineral N availability in this treatment. Overall, our study revealed that ensiled grass-clover was the best fertilizer product and that the method chosen for incorporation of green manure is likely to influence N₂O emissions.     

N2O and NO emissions from an Andisol field as influenced by pelleted poultry manure

Animal manures from intensive livestock operations can be pelleted to improve handlings and recyclings of embodied nutrients. The aim of this study was to evaluate the influence of pelleted poultry manure on N₂O and NO fluxes from an Andisol field. In autumn 2006 and summer 2007, poultry manure (PM), pelleted poultry manure (PP), and chemical fertilizer (CF) were applied at a rate of 120 kg N ha⁻¹ in each cultivation period to Komatsuna (Brassica rapa var. peruviridis). Nitrous oxide and NO fluxes were measured using an automated monitoring system. A soil incubation experiment was also conducted to determine the influence of intact and ground pelleted manure on N₂O, NO, and CO₂ production with a water-filled pore space (WFPS) of 30 or 50%. In the field measurements, N₂O emission rates from the organic fertilizer treatments were larger than that from the CF treatment, possibly because organic C stimulated denitrification. The highest N₂O flux was observed from the PP treatment after a rainfall following fertilization, and the cumulative emission rate (2.72 ± 0.22 kg N ha⁻¹ y⁻¹) was 3.9 and 7.1 times that from the PM and CF treatments, respectively. In contrast, NO emission rates were highest from the CF treatment. The NO/N₂O flux ratio indicated that nitrification was the dominant process for NO and N₂O production from the CF treatment. Cumulative N₂O emission rates from all treatments were generally higher during the wetter cultivation period (autumn 2006) than during the drier cultivation period (summer 2007). In contrast, NO emission rates were higher in the drier than in the wetter cultivation period. The incubation experiment results showed a synergistic effect of soil moisture and the pelleted manure form on N₂O emission rates. The intact pelleted manure with the 50% WFPS treatment produced the highest N₂O and CO₂ fluxes and resulted in the lowest soil NO₃⁻ content after the incubation. These results indicate that anaerobic conditions inside the pellets, caused by rainfall and heterotrophic microbial activities, led to denitrification, resulting in high N₂O fluxes. Controlling the timing of N application by avoiding wet conditions might be one mitigation option to reduce N₂O emission rates from the PP treatment in this study field.     

Linking Organic Carbon,Water Content,and Nitrous Oxide Emission in a Reclaimed Coal Mine Soil

Mine‐land reclamation for biomass production is often achieved by means of large applications of N and organic C with amendments that could create soil conditions favorable for N₂O production and emissions. To investigate this possibility, we conducted a laboratory experiment using mine soil collected from an active surface coal mine site near Philipsburg, Pennsylvania. During a 37‐d incubation period, we measured N₂O and CO₂ fluxes from non‐amended soil and from soil amended with ammonium nitrate (L + F), composted poultry manure (Comp), poultry manure alone (Man) and mixed with 3 rates of paper mill sludge (PMS) to obtain carbon to nitrogen ratios of 14, 20 and 27 (Man + PMS14, 20 and 27), each at 60% and 80% water filled pore space (WFPS). Results showed that manure alone leads to a greater emission of N₂O under laboratory conditions than with L + F. However, composting manure effectively reduced the emissions compared to that of L + F despite a large addition of organic C and N. Composted manure‐treated soil emitted less than all other manure‐based treatments at both 60% and 80% WFPS. The emissions were greater from soil amended with the Man + PMS treatments compared to non‐amended and L + F‐amended soil, and it increased during periods of intense microbial activity created by the application of manure and PMS. Higher water content increased emissions particularly during periods of intense microbial activity coupled with inorganic N availability. Cumulative N₂O emissions from manure‐treated soils represented less than 0·1% loss of total applied N. Copyright © 2014 John Wiley & Sons, Ltd.     

Effect of Adding Flue Gas Desulphurization Gypsum on the Transformation and Fate of Nitrogen during Composting

The objective of this study was to investigate the effect of adding flue gas desulphurization gypsum (FGDG) on the transformation and fate of nitrogen during co-composting of dairy manure and pressmud of a sugar refinery. The ammonia absorption of FGDG was investigated. The changes in compost temperature, pH, electrical conductivity (EC), moisture, organic matter, the C/N ratio, Kjeldahl N, NH₄⁺-N, NO₂^?-N, NO₃^?-N were assessed. The addition of FGDG did not significantly affect compost temperature, pH, EC, moisture, and organic matter degradation. However, the addition of FGDG significantly increased the NH₄⁺-N content in the compost during the thermophilic phase, and the NH₄⁺-N maximal content in the compost with FGDG (CP+G) was 59.9% more than that in the compost without FGDG (CP–G). FGDG was thought to create the formation of (NH₄)₂SO₄ and the cation exchange between NH₄⁺ and Ca²⁺. The NO₂^?-N content in the CP+G peaked on day 15, and was not observed in the CP–G. In the final compost products, the NO₃^?-N concentration in the CP–G was more than that in the CP+G, which was 1451 (CP–G) and 1109 mg·kg^?1 (CP+G) dry material. This might be due to the NO₂^? accumulation in the CP+G, which accelerated N loss in the form of N₂O. There is a strong correlation between N₂O emission and NO₂^?-N accumulation in the composting process. Compared with the original N content in the compost mixture, the N loss in CP–G and CP+G were 15.0 and 10.8%, respectively. These results revealed that NH₄⁺-N conservation effect was improved during the thermophilic phase and the total N loss was mitigated by adding FGDG into composting materials. FGDG could be utilized as a potential amendment to conserve nitrogen during composting.     

有机无机肥料配合施用对设施菜田土壤N2O排放的影响

采用静态箱气相色谱法研究了有机无机肥料配合施用对设施菜田土壤N2O排放的影响。结果表明: 1）设施芹菜和番茄施基肥后57 d（灌溉后13 d）出现土壤N2O排放通量峰值,追肥后（施肥与灌溉同步）1 d出现土壤N2O排放通量峰值; 芹菜季和番茄季施用基肥后20 d内N2O排放量分别占当季总排放量的40%65%左右,是土壤N2O主要排放期。2）施用基肥后至定植灌水前各处理土壤N2O排放量逐渐降低,灌水后N2O排放通量迅速上升。各处理土壤N2O排放通量与土壤含水量之间呈显著相关,相关系数在0.43~0.72之间。3）土壤N2O排放主要发生在番茄季,番茄生育期各处理土壤N2O总排放量是芹菜生育期的3.1倍; 各处理土壤N2O排放通量与5 cm土层温度之间总体上呈显著相关,相关系数在0.40~0.58之间。4）设施菜田大幅减施化肥的有机无机肥配合施用模式可显著降低土壤N2O排放量和肥料损失率,芹菜季和番茄季土壤N2O排放量较习惯施肥处理分别降低66.3%和85.1%,肥料损失率分别降低45.2%和74.9%。5）等氮量投入时,施用秸秆较施用猪粪可有效降低土壤N2O排放,芹菜季和番茄季分别降低43.4%和74.2%。     

Effect of nitrite accumulation on nitrous oxide emission and total nitrogen loss during swine manure composting

Abstract

The present study investigated the nitrogen balance in swine manure composting to evaluate the effect of nitrite (NO^? ₂) accumulation, which induces nitrogenous emissions, such as N₂O, during compost maturation. During active composting, most N losses result from NH₃ emission, which was 9.5% of the initial total nitrogen (TN_initial), after which, NO^? ₂ began to accumulate as only ammonia-oxidizing bacteria proliferated. After active composting, the addition of mature swine compost (MSC), including nitrite-oxidizing bacteria (NOB), could prevent NO^? ₂ accumulation and reduce N₂O emission by 70% compared with the control in which NO^? ₂ accumulated as a result of delayed growth of indigenous NOB. Total N₂O emissions in the control and in the treatment of MSC addition (MA) were 9.3% and 3.0% of TN_initial, respectively, whereas N losses as the sum total of NH₃ and N₂O over the whole period were 19.0% (control) and 12.8% (MA) of TN_initial, respectively. However, the difference in total N losses was markedly greater than that measured as NH₃ and N₂O, which were 27.8% (control) and 13.3% (MA) of TN_initial, respectively. These results demonstrated that the magnitude of nitrogen losses induced by NO^? ₂ accumulation is too large to ignore in the composting of swine manure.     

Effect of Woody Peat as an Additive on Maturity and Gaseous Emissions During Pig Manure Composting

Woody peat was used as an additive to compost with pig manure in 1.2 m³ composting reactors under aerobic conditions for a 77?days period to estimate the effect on the compost maturity and gaseous emissions (NH₃, N₂O, and CH₄). Pig manure was also composted with cornstalks (the traditional method) as a control treatment. The results showed that both cornstalks and woody peat composts reached the required maturity standard. Composting with woody peat as a bulking agent was found to reduced NH₃ emissions by 36% than the cornstalks amended treatment. Although CH₄ emission increased by adding woody peat, N₂O emission was considerably reduced, resulting in a slight decrease in total greenhouse gas emissions. More importantly, woody peat could reduce the losses of total carbon and total nitrogen, improve the compost quality as fertilizer.     

Residual Effects of Novel versus Traditional Organic Amendments for Rain-fed No-till Barley: Yield,Nutrient Uptake,and N2O Emissions

Organic amendments recycle nutrients, but N₂O emissions are both environmental and agronomic concerns. We conducted a 4-year field experiment to determine no-till barley (Hordeum vulgare L.) yield and nutrient uptake and soil N₂O emissions following a single application of six amendment treatments: (1) no amendment (Check); (2) synthetic N fertilizer (Fert); (3) fresh beef cattle feedlot manure (ManureF); (4) beef cattle feedlot manure compost (CompostR); (5) beef cattle feedlot manure composted with cattle mortalities (CompostM); and (6) separated solids from anaerobically digested cattle feedlot manure (ADM). Barley grown in Year 1 (2006), Year 2 (2007), and Year 4 (2009) (with Year 3 (2008) under fallow) had higher grain yields from ManureF (4.73 Mg ha^?1) in Year 2 and ADM (6.30 Mg ha^?1) in Year 4 (p < 0.05) than other treatments. The grain N and P contents were not affected (p > 0.05), but N uptake over 3 years (112.8 kg N ha^?1 yr^?1), and P uptake in Year 1 (19.1 kg ha^?1 yr^?1) and Year 2 (14.3 kg ha^?1 yr^?1) from ManureF, were higher (p < 0.05×) than other treatments. The cumulative N₂O emissions from ManureF in Year 1 (1.488 kg N ha^?1) and from ADM in Year 2 (1.072 kg N ha^?1) were higher (p < 0.05) than other treatments while the fraction of applied N emitted as N₂O was small (0.00 to 0.79%) and not affected by treatment. However, the percentages of applied N emitted as N₂O from compost and ADM were similar to synthetic fertilizer and livestock manure.     

施肥方式对冬小麦—夏玉米轮作土壤N_2O排放的影响

氧化亚氮(N_2O)是一种重要的农田温室气体,本研究利用紫色土长期施肥试验平台,采用静态箱/气相色谱法对紫色土旱作农田冬小麦—夏玉米轮作系统的N_2O排放进行了定位观测(2012年11月至2013年9月),研究单施氮肥(N)、常规氮磷钾肥(NPK)、猪厩肥(OM)、猪厩肥配施氮磷钾肥(OMNPK)和秸秆还田配施氮磷钾肥(ICRNPK)等施肥方式对紫色土N_2O排放特征的影响;不施肥(NF)作为对照计算排放系数,以探寻紫色土地区可操作性强、环境友好的施肥方式。结果表明,所有施肥方式的N_2O排放均呈现双峰排放,峰值出现在施肥初期;玉米季N_2O排放峰值显著高于小麦季(p0.05)。在相同的施氮水平(小麦季130 kg hm~(~(-2)),玉米季150 kg hm~(~(-2)))下,施肥方式对N_2O排放和作物产量均有显著影响(p0.05)。N、OM、NPK、OMNPK和ICRNPK处理的土壤N_2O周年累积排放量分别为1.93、1.96、1.12、1.50和0.79 kg hm~(~(-2)),排放系数分别为0.62%、0.63%、0.33%、0.47%和0.21%,全年作物产量分别为4.35、11.95、8.39、9.77、10.93 t hm~(~(-2))。施用猪厩肥显著增加N_2O排放量,而秸秆还田在保证作物产量的同时显著降低N_2O排放量,可作为紫色土地区环境友好的施肥方式。土壤无机氮(NO_3~--N和NH_4~+-N)是N_2O排放的主要限制因子。因此,在施氮水平相同时,施肥方式对紫色土活性氮含量的影响导致N_2O排放差异显著,是土壤N_2O排放差异的根本原因。土壤孔隙充水率也是影响N_2O排放的重要环境因子,并且其对N_2O排放的影响存在阈值效应。     

NH3 Volatilization,N2O Emission and Microbial Biomass Turnover from 15N-Labeled Manure Under Laboratory Conditions

On irrigated agricultural soils from semi-arid and arid regions, ammonia (NH₃) volatilization and nitrous oxide (N₂O) emission can be a considerable source of N losses. This study was designed to test the capture of ¹⁵N loss as NH₃ and N₂O from previous and recent manure application using a sandy, calcareous soil from Oman amended one or two times with ¹⁵N labeled manure to elucidate microbial turnover processes under laboratory conditions. The system allowed to detect ¹⁵N enrichments in evolved N₂O-N and NH₃-N of up to 17% and 9%, respectively, and total N, K₂SO₄ extractable N and microbial N pools from previous and recent ¹⁵N labeled manure applications of up to 7%, 8%, and 15%. One time manured soil had higher cumulative N₂O-N emissions (141 µg kg^?1) than repeatedly manured soil with 43 µg kg^?1 of which only 22% derived from recent manure application indicating a priming effect.     

Nitrous oxide emissions under a four-year crop rotation system in northern Japan: impacts of reduced tillage,composted cattle manure application and increased plant residue input

In the context of their role in global warming, nitrous oxide (N₂O) emissions from agricultural soil under different management practices were studied in Hokkaido, northern Japan. To assess the impacts of reduced tillage, composted cattle manure-based fertilization and amendments with crop residues and green manure on N₂O emissions from soil, a field experiment was conducted under a four-year crop rotation on a well-drained Andisol. The crop rotation included potato (Solanum tuberosum L.) or sweet corn (Zea mays L.), winter wheat (Triticum aestivum L.), sugar beet (Beta vulgaris L. subsp. vulgaris) and soybean (Glycine max (L.) Merr.). The cumulative N₂O emissions for the four-year study period differed widely (0.33 to 4.90?kg?N?ha^?1), depending on the treatments imposed, being the greatest for a combination of conventional moldboard plow tillage, composted cattle manure-based fertilization and increased plant residue input, and the lowest for a combination of conventional tillage, chemical fertilizer-based fertilization and normal plant residue input treatments. The cumulative N₂O emissions under reduced tillage were all small, irrespective of fertilization and plant residue input treatments. Composted cattle manure-based fertilization (P?≤?0.01) and increased plant residue input (P?≤?0.01) significantly increased cumulative N₂O emissions. Tillage showed a significant interaction with fertilization and plant residue input, indicating that N₂O emissions were enhanced when composted cattle manure, crop residues and green manure were incorporated by conventional tillage. In the present study, the N₂O emission factors for chemical fertilizer, composted cattle manure and crop residues were 0.26?±?0.44, 0.11?±?0.16 and ?0.03?±?0.52%, respectively, all much lower than the country-specific emission factor for Japan's well-drained soils (0.62%) and the default emission factor used in the IPCC guideline (1%).     

Nitrous oxide and methane emissions from grassland treated with bark- or sawdust-containing manure at different rates

On the main Japanese island of Honshu, bark or sawdust is often added to cattle excreta as part of the composting process. Dairy farmers sometimes need to dispose of manure that is excess to their requirements by spreading it on their grasslands. We assessed the effect of application of bark- or sawdust-containing manure at different rates on annual nitrous oxide (N₂O) and methane (CH₄) emissions from a grassland soil. Nitrous oxide and CH₄ fluxes from an orchardgrass (Dactylis glomerata L.) grassland that received this manure at 0, 50, 100, 200, or 300?Mg?ha^?1?yr^?1 were measured over a two-year period by using closed chambers. Two-way analysis of variance (ANOVA) was employed to examine the effect of annual manure application rates and years on annual N₂O and CH₄ emissions. Annual N₂O emissions ranged from 0.47 to 3.03?kg?N?ha^?1?yr^?1 and increased with increasing manure application rate. Nitrous oxide emissions during the 140-day period following manure application increased with increasing manure application rate, with the total nitrogen concentration in the manure, and with cumulative precipitation during the 140-day period. However, manure application rate did not affect the N₂O emission factors of the manure. The overall average N₂O emission factor was 0.068%. Annual CH₄ emissions ranged from ?1.12 to 0.01?kg?C?ha^?1?yr^?1. The annual manure application rate did not affect annual CH₄ emissions.     

Nitrous oxide emissions and nitrogen cycling in managed grassland in Southern Hokkaido,Japan

Abstract

Nitrous oxide (N₂O) emissions were measured and nitrogen (N) budgets were estimated for 2?years in the fertilizer, manure, control and bare plots established in a reed canary grass (Phalaris arundinacea L.) grassland in Southern Hokkaido, Japan. In the manure plot, beef cattle manure with bark was applied at a rate of 43–44?Mg fresh matter (236–310?kg?N)?ha^?1?year^?1, and a supplement of chemical fertilizer was also added to equalize the application rate of mineral N to that in the fertilizer plots (164–184?kg?N?ha^?1?year^?1). Grass was harvested twice per year. The total mineral N supply was estimated as the sum of the N deposition, chemical fertilizer application and gross mineralization of manure (GMm), soil (GMs), and root-litter (GMl). GMm, GMs and GMl were estimated by dividing the carbon dioxide production derived from the decomposition of soil organic matter, root-litter and manure by each C?:?N ratio (11.1 for soil, 15.5 for root-litter and 23.5 for manure). The N uptake in aboveground biomass for each growing season was equivalent to or greater than the external mineral N supply, which is composed of N deposition, chemical fertilizer application and GMm. However, there was a positive correlation between the N uptake in aboveground biomass and the total mineral N supply. It was assumed that 58% of the total mineral N supply was taken up by the grass. The N supply rates from soil and root-litter were estimated to be 331–384?kg?N?ha^?1?year^?1 and 94–165?kg?N?ha^?1?year^?1, respectively. These results indicated that the GMs and GMl also were significant inputs in the grassland N budget. The cumulative N₂O flux for each season showed a significant positive correlation with mineral N surplus, which was calculated as the difference between the total mineral N supply and N uptake in aboveground biomass. The emission factor of N₂O to mineral N surplus was estimated to be 1.2%. Furthermore, multiple regression analysis suggested that the N₂O emission factor increased with an increase in precipitation. Consequently, soil and root-litter as well as chemical fertilizer and manure were found to be major sources of mineral N supply in the grassland, and an optimum balance between mineral N supply and N uptake is required for reducing N₂O emission.     

Primed N2O emission from native soil nitrogen: A 15N‐tracing laboratory experiment

Soils can naturally be a source of the potent greenhouse gas nitrous oxide (N₂O). By contrast, the largest anthropogenic source of N₂O is the application of nitrogen (N) fertilizer on agricultural soil, but it is unclear if fertilizer‐supported N₂O emission only originates from the fertilizer N directly or through additionally stimulated N₂O production from native soil N. Even though native soil N also includes mineral N already in soil before fertilizer application, organic N is the principal native N pool and thereby provides for mineral N cycling and N₂O emission. Here, we tested (1) the contribution of native soil N to N₂O emission after mineral N fertilizer application and (2) whether it is affected by different soil organic matter (SOM) contents by conducting a laboratory ¹⁵N‐tracing experiment with agricultural soil from a long‐term field trial with two treatments. Both field treatments are fertilized with mineral N, whereas only one of the two receives liquid manure causing higher SOM content. Soil sampling was conducted in March 2016 shortly before fertilizer application in the field. The application of ¹⁵N‐labeled fertilizer more than doubled the N₂O production from native N sources compared to the non‐fertilized control incubations. This primed N₂O production contributed by 5–8% to the fertilizer‐induced N₂O emission after one week of incubation and was similar for both field treatments regardless of liquid manure application. Therefore, further research is needed to link N₂O priming to its potential production pathways and sources. While the observed effect may be important in soils, the amount of applied N fertilizer remains the largest concern being responsible for the majority of N₂O emission.