有机无机肥配施对玉米-豇豆种植系统土壤N2O排放的影响

在等施氮量条件下,比较有机肥与无机肥施用后旱地玉米-豇豆复种系统土壤硝化与反硝化作用、N_2O排放与作物产量的变化,有助于正确认识肥料施用对N_2O排放的影响,为制定大田合理的丰产减排措施提供理论依据。本研究通过田间试验,利用静态箱技术和BaPS气压过程分离技术研究了不同肥料类型处理(无机肥、有机肥、有机无机肥配施)下玉米-豇豆种植系统土壤N_2O排放、硝化与反硝化作用的变化特征。结果表明:1)相对于单施无机肥或有机肥,有机无机肥配施可显著降低土壤硝化作用速率;在玉米生长季,有机无机肥配施处理平均土壤硝化作用速率分别比化肥和有机肥处理显著降低了28.74%和13.96%,豇豆生长季显著降低了24.66%和13.28%。土壤反硝化作用速率在各施肥处理间差异不显著。2)有机无机肥配施显著降低土壤N_2O排放;在玉米生长季,有机无机肥配施处理分别比无机肥处理和有机肥处理显著降低33.44%和32.29%,在豇豆生长季分别显著降低27.00%和15.14%。3)相关分析表明,土壤N_2O排放与硝化作用速率呈极显著相关,而与反硝化作用速率呈不显著相关。4)有机无机配施处理玉米和豇豆产量最高。因此,有机无机肥配施能有效降低玉米-豇豆系统土壤N_2O排放和提高作物产量,是一项丰产低N_2O排放的施肥技术,但长期有机无机肥配施对土壤N_2O排放和作物产量的影响还需要进一步研究。     

配施有机肥对潜育化水稻土的培肥效果

为探索潜育化水稻土的培肥措施,通过田间试验研究了有机无机肥配施对潜育化低产水稻土产量和土壤养分有效性的影响。结果显示:连续2季施用有机肥后,潜育化水稻土产量显著提高,以早稻配施紫云英-晚稻配施猪粪处理的产量最高。添加有机肥降低了潜育化水稻土耕层土壤p H值。早稻配施紫云英,晚稻配施猪粪处理的有机质提高明显,比NPK处理提高了15.22%;早稻配施紫云英,晚稻配施猪粪处理的潜育化水稻土全氮含量比单施化肥处理提高4.19%;早稻配施紫云英,晚稻单施化肥处理的潜育化水稻土有效磷含量提高最多,比NPK处理提高了4.92%;相比于NPK处理,早稻配施紫云英,晚稻单施化肥处理的土壤速效钾含量比两季均单施化肥处理提高了13.2%。因此,潜育化水稻土适当配施有机肥可以提高土壤养分有效性,增加水稻产量,可以作为培肥改良潜育化水稻土的参考方法。     

长期施肥对黑土氮素矿化与硝化作用特征的影响

采用培养试验研究了长期施肥对黑土矿化与硝化作用特征的影响。结果表明,黑土的矿化作用和硝化作用都较强,长期施肥对黑土矿质态氮量有显著影响,施用化肥能够增加矿质态氮量,在施用NPK肥基础上增施有机肥,矿质态氮量进一步增加,表明在土壤管理上如果增加有机肥的施用,可以提高土壤的供氮能力。长期施肥黑土的硝化率与施N肥相关性较好,其次是施用PK肥。有机肥与无机肥配施可使土壤硝化率显著提高;硝化率高低取决于黑土可矿化态氮素含量和土壤pH。     

不同有机物及其堆肥与化肥配施对小麦生长及氮素吸收的影响

采用15N示踪技术,选用水稻土和灰潮土在宜兴进行小麦盆栽试验,研究了稻草、猪粪及其堆肥与化肥配施对作物生长及氮素吸收的影响。结果表明,在水稻土和灰潮土上,不同有机物及其堆肥与化肥配施分别比单施化肥增产4.46%～24.82%和1.01%～20.53%,稻草堆肥和猪粪堆肥配施化肥处理籽粒产量分别高于稻草和猪粪直接与化肥配施处理。稻草和猪粪堆肥后更利于作物吸收氮素,增加植物体内15N累积。两种土壤上15N回收率表现为相同配比的堆肥处理未堆肥处理单施化肥处理。随着小麦生育期的推进,土壤微生物量氮和矿质态氮含量均呈下降趋势,稻草和猪粪处理的微生量氮含量始终高于稻草堆肥和猪粪堆肥处理。有机无机肥配施处理土壤矿质态氮在小麦生育前期低于单施化肥,成熟期则高于单施化肥。整个生育期中,稻草堆肥和猪粪堆肥处理土壤矿质态氮含量分别高于稻草和猪粪处理。因此,有机物堆肥后与化肥配施更有利于提高产量,促进作物对氮素的吸收利用。     

有机无机氮肥配施对莴苣土壤N2O排放的影响

采用静态箱–气相色谱法研究不同种类有机无机氮肥配合施用对盆栽莴苣土壤N_2O排放规律及排放量的影响。试验设置不施肥(CK)、不施氮肥(PK)、施纯化肥(NPK)、有机无机肥配施1(20%猪粪氮+80%化肥氮,NPKM1)、有机无机肥配施2(20%沼渣沼液氮+80%化肥氮,NPKM2)和有机无机肥配施3(20%猪粪堆肥氮+80%化肥氮,NPKM3)共6个处理。结果表明:莴苣生育期各处理施肥后土壤N_2O排放出现多个峰值,出峰时间和大小不一;累积排放量随着生育期的进程逐渐增加,处理间差异更为明显。莴苣生育期各处理土壤N_2O平均排放通量及累积排放量范围分别为0.10～0.25 mg/(m2·h)和1.37～3.42 kg/hm2,大小均表现为NPKNPKM2PKNPKM1NPKM3CK。土壤N_2O排放系数范围为0.13%～0.68%,大小表现为NPKNPKM2NPKM1NPKM3。与NPK处理相比,NPKM1、NPKM2和NPKM3处理莴苣土壤N_2O累积排放量均分别降低48.08%、25.75%和48.30%,产量分别增加48.66%、22.13%和53.76%。总之,施用纯化肥会促进菜地土壤N_2O的排放,而不同种类有机无机氮肥配施能有效减少N_2O排放且提高作物产量,以猪粪类配施效果最佳。因此,有机无机配施是菜地N_2O减排、降低蔬菜种植中氮素损失的重要途径。     

浅层淹水条件下不同施肥处理对黑土氮素净转化的影响

以东北旱作黑土为对象,在25℃和浅层淹水条件下开展了为期35 d的室内培养试验,研究不同施肥处理对黑土矿化作用和硝化作用的影响。结果表明,浅层淹水条件下土壤矿化作用和硝化作用仍能进行。与不施肥对照处理相比,施用氮肥抑制了培养初期的有机氮矿化,但对后期氮矿化没有影响。氮肥施用初期对硝化作用没有影响,但2周后显著促进了硝化作用的进行。培养期间单施氮肥处理的平均净矿化速率为N 1.07 mg/(kg·d),与对照处理没有显著差异;平均净硝化速率为N 4.50 mg/(kg·d),是对照处理的2.43倍。浅层淹水条件下氮肥配施有机物料显著促进了土壤无机氮的生物固定,培养初期氮肥配施秸秆处理的无机氮固定量大于氮肥配施猪粪处理,后期则相对稍低,氮肥配施猪粪和配施秸秆处理的平均净氮矿化速率分别为N-5.61和-3.15 mg/(kg·d),两者间差异显著。与单施氮肥处理相比,浅层淹水条件下氮肥配施有机物料显著抑制了土壤硝化作用,培养期间氮肥配施猪粪和氮肥配施秸秆处理的平均净硝化速率分别为N 0.29和0.18 mg/(kg·d),分别比单施氮肥处理下降了93.5%和96.0%。     

配施有机肥提高化肥氮利用效率的微生物作用机制研究

采用15N示踪技术和盆栽试验研究了水稻生长期间不同施肥处理土壤微生物量氮的动态变化,探讨了配施有机肥提高化肥氮利用率的微生物作用机制。结果表明,在水稻生育前期,化肥配施鸡粪堆肥、猪粪堆肥和酒糟堆肥较化肥单施均提高了土壤微生物对化肥15N的固持率,降低了土壤矿质态15N含量。而随水稻生育进程推进,在先前被微生物固持的化肥15N化肥配施鸡粪堆肥、猪粪堆肥和酒糟堆肥处理分别有87%、81%和81%被释放,增加了同期水稻对化肥15N的吸收量。化肥配施鸡粪堆肥、猪粪堆肥和酒糟堆肥,化肥15N利用率均超过60%,而单施化肥利用率仅39%。可见,配施有机肥提高化肥氮利用率,其机制之一是通过促进土壤微生物对化肥氮的有效调控,使化肥氮更好地被转化利用。     

太湖地区黄泥土表层氮库变化与水稻产量间的关系研究

24年田间定位试验研究表明,长期不施肥处理和化肥PK配施处理土壤表层N呈下降趋势,而施入化肥N处理(包括化肥N、化肥NP配施、化肥NK配施、化肥NPK配施处理)N增加较缓慢,有机与无机肥结合处理土壤表层N增加迅速。以长期不施肥为对照,长期有机肥 NPK处理水稻增产最多,达35.94%。随着N肥的逐年施入,土壤表层全N含量与产量间由显著正相关关系变为相关性不显著。     

有机无机氮肥配施对菜地土壤二氧化碳和甲烷排放的影响

采用静态箱—气相色谱法研究不同种类有机无机肥料配合施用对盆栽莴苣土壤二氧化碳(CO_2)和甲烷(CH_4)排放规律及排放量的影响。以等氮(N)用量为基准,磷(P)、钾(K)用量相应折算,分别以猪粪、沼渣沼液、猪粪堆肥与化肥进行配施,且各处理N、P、K总施用量一致。共设置6个处理:不施肥(CK);不施氮肥(PK);施纯化肥(NPK);有机无机肥配施1(20%猪粪N+80%化肥N,NPKM1);有机无机肥配施2(20%沼渣沼液N+80%化肥N,NPKM2);有机无机肥配施3(20%猪粪堆肥N+80%化肥N,NPKM3)。结果表明:莴苣全生育期内土壤CH_4排放各处理呈现"源"、"汇"交替现象,各时间测点排放通量在每次施肥后均呈现升降波动趋势,直至总体趋于平稳;各阶段排放累积量随着生育期的变化,处理间差异逐渐明显。莴苣盆栽土壤CH_4累积排放量为-5.90～-0.42 kg·hm~(-2),大小表现为CK=PKNPKNPKM3NPKM1NPKM2。莴苣全生育期内土壤CO_2的各时间测点排放通量总体呈现上升趋势,受施肥影响略有波动;各阶段排放累积量随着生育期的增加,处理间差异更加明显。莴苣盆栽土壤CO_2累积排放量为1 506.37～2 260.58 kg·hm~(-2),大小表现为NPKM3NPKM1NPKNPKM2PKCK。莴苣盆栽全球增温潜势为1.50～2.23 t CO_2eq·hm~(-2),大小表现为NPKM3NPKM1NPKNPKM2PKCK。总体而言,不同有机无机肥料配施表现为菜地土壤的CH_4汇,以沼渣沼液配施效果最佳,而猪粪类有机肥配施会导致菜地全球增温潜势升高,应合理搭配进行施用。     

长期施肥下黑土玉米田土壤温室气体的排放特征

研究不同施肥措施下东北黑土区玉米农田温室气体(CO_2、N_2O和CH_4)的排放量及其增温潜势,将为制定农业温室气体减排措施提供理论依据。本研究以国家(公主岭)黑土长期定位试验为平台,采用静态箱-气相色谱法对不同施肥措施下玉米农田土壤温室气体排放通量进行了监测,并分析了不同施肥处理间玉米田的综合温室效应差异。结果表明:各施肥处理土壤温室气体CO_2和N_2O的排放高峰均出现在玉米拔节期。农家肥和化肥配施(M_2NPK)处理土壤CO_2、N_2O排放通量和CH_4吸收量均显著高于施化肥处理(P0.05);施用化肥处理土壤CO_2、N_2O排放通量高于不施肥处理;撂荒区土壤CO_2排放通量最高,而土壤N_2O排放通量显著低于施肥处理;等施氮量条件下,化肥(NPK)处理土壤N_2O排放通量明显高于秸秆还田(SNPK)处理,而土壤CH4净吸收量结果则截然相反。从土壤综合温室效应和温室气体强度可分析出,与不施肥(CK)比较,偏施化肥N和NPK处理的综合温室效应(GWP)分别增加了142%和32%,SNPK综合温室效应降低了38%;尤其是有机无机配施(M_2NPK)处理的综合温室效应为负值,为净碳汇。平衡施肥NPK和有机无机肥配施(SNPK和M_2NPK)温室气体排放强度(GHGI)较弱,显著低于不施肥(CK)和偏施化肥(N)处理,其中M2NPK为-222 kg CO_2-eq·t~(-1)。因此,为同步实现较高的玉米产量和较低的温室气体排放强度,有机无机肥配施是东北黑土区较为理想的土壤培肥方式。     

施肥方式对紫色土农田生态系统N2O和NO排放的影响

依托紫色土施肥方式与养分循环长期试验平台(2002年—),采用静态箱-气相色谱法开展紫色土冬小麦-夏玉米轮作周期(2013年10月至2014年10月)农田生态系统N_2O和NO排放的野外原位观测试验。长期施肥方式包括单施氮肥(N)、传统猪厩肥(OM)、常规氮磷钾肥(NPK)、猪厩肥配施氮磷钾肥(OMNPK)和秸秆还田配施氮磷钾肥(RSDNPK)等5种,氮肥用量相同[小麦季130 kg(N)×hm~(-2),玉米季150 kg(N)×hm~(-2)],不施肥对照(CK)用于计算排放系数,对比不同施肥方式对紫色土典型农田生态系统土壤N_2O和NO排放的影响,以期探寻紫色土农田生态系统N_2O和NO协同减排的施肥方式。结果表明,所有施肥方式下紫色土N_2O和NO排放速率波动幅度大,且均在施肥初期出现峰值;强降雨激发N_2O排放,但对NO排放无明显影响。在整个小麦-玉米轮作周期,N、OM、NPK、OMNPK和RSDNPK处理的N_2O年累积排放量分别为1.40 kg(N)×hm~(-2)、4.60 kg(N)×hm~(-2)、0.95 kg(N)×hm~(-2)、2.16kg(N)×hm~(-2)和1.41 kg(N)×hm~(-2),排放系数分别为0.41%、1.56%、0.25%、0.69%、0.42%;NO累积排放量分别为0.57 kg(N)×hm~(-2)、0.40 kg(N)×hm~(-2)、0.39 kg(N)×hm~(-2)、0.46 kg(N)×hm~(-2)和0.17 kg(N)×hm~(-2),排放系数分别为0.21%、0.15%、0.15%、0.17%、0.07%。施肥方式对紫色土N_2O和NO累积排放量具有显著影响(P0.05),与NPK处理比较,OM和OMNPK处理的N_2O排放分别增加384%和127%,同时NO排放分别增加3%和18%;RSDNPK处理的NO排放减少56%。表明长期施用猪厩肥显著增加N_2O和NO排放,而秸秆还田有效减少NO排放。研究表明,土壤温度和水分条件均显著影响小麦季N_2O和NO排放(P0.01),对玉米季N_2O和NO排放没有显著影响(P0.05),土壤无机氮含量则是在小麦-玉米轮作期N_2O和NO排放的主要限制因子(P0.01)。全量秸秆还田与化肥配合施用是紫色土农田生态系统N_2O和NO协同减排的优化施肥方式。     

施肥对夏玉米季紫色土N2O排放及反硝化作用的影响

采用原状土柱-乙炔抑制培养法研究了施肥对紫色土玉米生长季土壤N2O排放通量和反硝化作用的影响.结果表明:玉米季施肥显著增加土壤N2O排放和反硝化损失,同时,各施肥处理间N2O排放与反硝化损失量差异显著.猪厩肥、猪厩肥配施氮磷钾肥、氮肥、氮磷钾肥和秸秆配施氮磷钾肥等处理的土壤N,O排放量分别为3.01、2.86、2.51、2.19和1.88 kg hm-2,分别占当季氮肥施用量的1.63％、1.53％、1.30％、1.09％和0.88％,反硝化损失量分别为6.74、6.11、5.23、4.69和4.12 kg hm-2,分别占当季氮肥施用量的3.97％、3.55％、2.97％、2.61％和2.23％,不施肥土壤的N2O排放量和反硝化损失量仅为0.56和0.78 kg hm-2.施肥是紫色土玉米生长前期(2周内)土壤N2O排放和反硝化速率出现高峰的主要驱动因子,土壤铵态氮和硝态氮含量是影响土壤N2O排放、土壤硝化和反硝化作用的限制因子,土壤含水量是重要影响因子,降雨是主要促发因素.土壤N2O排放量与反硝化损失量的比值介于0.45 ～0.72之间,土壤反硝化损失量极显著高于土壤N2O排放量,说明土壤反硝化作用是紫色土玉米生长季氮肥损失的重要途径.     

Linkage of N2O emissions to the abundance of soil ammonia oxidizers and denitrifiers in purple soil under long-term fertilization

Abstract

Microbial nitrification and denitrification are responsible for the majority of soil nitrous (N₂O) emissions. In this study, N₂O emissions were measured and the abundance of ammonium oxidizers and denitrifiers were quantified in purple soil in a long-term fertilization experiment to explore their relationships. The average N₂O fluxes and abundance of the amoAgene in ammonia-oxidizing bacteria during the observed dry season were highest when treated with mixed nitrogen, phosphorus and potassium fertilizer (NPK) and a single N treatment (N) using NH₄HCO₃as the sole N source; lower values were obtained using organic manure with pig slurry and added NPK at a ratio of 40%:60% (OMNPK),organic manure with pig slurry (OM) and returning crop straw residue plus synthetic NH₄HCO₃fertilizer at a ratio of 15%:85% (SRNPK). The lowest N₂O fluxes were observed in the treatment that used crop straw residue(SR) and in the control with no fertilizer (CK). Soil NH₄⁺provides the substrate for nitrification generating N₂O as a byproduct. The N₂O flux was significantly correlated with the abundance of the amoA gene in ammonia-oxidizing bacteria (r = 0.984, p < 0.001), which was the main driver of nitrification. During the wet season, soil nitrate (NO₃^?) and soil organic matter (SOC) were found positively correlated with N₂O emissions (r = 0.774, p = 0.041 and r = 0.827, p = 0.015, respectively). The nirS gene showed a similar trend with N₂O fluxes. These results show the relationship between the abundance of soil microbes and N₂O emissions and suggest that N₂O emissions during the dry season were due to nitrification, whereas in wet season, denitrification might dominate N₂O emission.     

施肥方式对冬小麦季紫色土N2O排放特征的影响

利用紫色土养分循环长期定位施肥试验平台,通过静态箱-气相色谱法,于2012年11月至2013年5月,研究了单施氮肥(N)、猪厩肥(OM)、常规氮磷钾肥(NPK)、猪厩肥配施氮磷钾肥(OMNPK)、秸秆还田配施氮磷钾肥(CRNPK)及对照不施肥(NF)6种施肥方式下,紫色土冬小麦季土壤N2O的排放特征。结果表明,在相同施氮水平[130 kg(N)·hm-2]下,施肥方式对N2O排放量有显著影响(P0.05)。N、OM、NPK、OMNPK和CRNPK处理下,土壤N2O排放量[kg(N)·hm-2]分别为0.38、0.36、0.29、0.33和0.19,N2O排放系数分别为0.25%、0.23%、0.18%、0.21%和0.10%。NF的土壤N2O排放量为0.06 kg(N)·hm-2。土壤无机氮含量(NO3--N和NH4+-N)是N2O排放的主要影响因子,降雨能有效激发N2O排放。基于小麦产量评价不同施肥方式下的N2O排放,结果表明,N、OM、NPK、OMNPK和CRNPK单位小麦产量N2O的GWP值[yield-scaled GWP,kg(CO2 eq)·t-1]分别为132.57、45.70、49.07、48.92和26.41。CRNPK的小麦产量与6种施肥方式中获得最大产量的OM间没有显著差异,但显著高于其他处理。而且,CRNPK的yield-scaled GWP比紫色土地区冬小麦种植中常规施肥方式(NPK)显著减少46%,并显著低于其他4种施肥方式。可见,秸秆还田配施氮磷钾肥在保证小麦产量的同时,能有效减少因施肥引发的N2O排放,可作为紫色土地区推荐的最佳施肥措施。     

Impact of Long-Term Application of Fertilizers on N2O and NO Production Potential in an Intensively Cultivated Sandy Loam Soil

Literature reports on N₂O and NO emissions from organic and mineral agricultural soil amended with N-containing fertilizers have reached contradictory conclusions. To understand the influence of organic manure (OM) and chemical fertilizer application on N₂O and NO emissions, we conducted laboratory incubation experiments on an agricultural sandy loam soil exposed to different long-term fertilization practices. The fertilizer treatments were initiated in 1989 at the Fengqiu State Key Agro-ecological Experimental Station and included a control without fertilizer (CK), OM, mineral NPK fertilizer (NPK), mineral NP fertilizer (NP), and mineral NK fertilizer (NK). The proportion of N emitted as NO and N₂O varied considerably among fertilizer treatments, ranging from 0.83% to 2.50% as NO and from 0.08% to 0.36% as N₂O. Cumulative NO emission was highest in the CK treatment after NH ₄ ⁺ -N was added at a rate of 200 mg N kg^?1 soil during the 612-h incubation period, whereas the long-term application of fertilizers significantly reduced NO emission by 54–67%. In contrast, the long-term application of NPK fertilizer and OM significantly enhanced N₂O emission by 95.6% and 253%, respectively, compared to CK conditions. The addition of NP fertilizer (no K) significantly reduced N₂O emission by 25.5%, whereas applications of NK fertilizer (no P) had no effect. The difference among the N-fertilized treatments was due probably to discrepancies in the N₂O production potential of the dominant ammonia-oxidizing bacteria (AOB) species rather than AOB abundance. The ratio of NO/N₂O was approximately 24 in the CK treatment, significantly higher than those in the N-fertilized treatments (3–11), and it decreased with increasing N₂O production potential in N-fertilized treatments. Our data suggests that the shift in the dominant AOB species might produce reciprocal change in cumulative NO and N₂O emissions.     

施肥方式对冬小麦—夏玉米轮作土壤N_2O排放的影响

氧化亚氮(N_2O)是一种重要的农田温室气体,本研究利用紫色土长期施肥试验平台,采用静态箱/气相色谱法对紫色土旱作农田冬小麦—夏玉米轮作系统的N_2O排放进行了定位观测(2012年11月至2013年9月),研究单施氮肥(N)、常规氮磷钾肥(NPK)、猪厩肥(OM)、猪厩肥配施氮磷钾肥(OMNPK)和秸秆还田配施氮磷钾肥(ICRNPK)等施肥方式对紫色土N_2O排放特征的影响;不施肥(NF)作为对照计算排放系数,以探寻紫色土地区可操作性强、环境友好的施肥方式。结果表明,所有施肥方式的N_2O排放均呈现双峰排放,峰值出现在施肥初期;玉米季N_2O排放峰值显著高于小麦季(p0.05)。在相同的施氮水平(小麦季130 kg hm~(~(-2)),玉米季150 kg hm~(~(-2)))下,施肥方式对N_2O排放和作物产量均有显著影响(p0.05)。N、OM、NPK、OMNPK和ICRNPK处理的土壤N_2O周年累积排放量分别为1.93、1.96、1.12、1.50和0.79 kg hm~(~(-2)),排放系数分别为0.62%、0.63%、0.33%、0.47%和0.21%,全年作物产量分别为4.35、11.95、8.39、9.77、10.93 t hm~(~(-2))。施用猪厩肥显著增加N_2O排放量,而秸秆还田在保证作物产量的同时显著降低N_2O排放量,可作为紫色土地区环境友好的施肥方式。土壤无机氮(NO_3~--N和NH_4~+-N)是N_2O排放的主要限制因子。因此,在施氮水平相同时,施肥方式对紫色土活性氮含量的影响导致N_2O排放差异显著,是土壤N_2O排放差异的根本原因。土壤孔隙充水率也是影响N_2O排放的重要环境因子,并且其对N_2O排放的影响存在阈值效应。     

Decreased nitrous oxide emissions associated with functional microbial genes under bio-organic fertilizer application in vegetable fields

Bio-organic fertilizers enriched with plant growth-promoting microbes(PGPMs)have been widely used in crop fields to promote plant growth and maintain soil microbiome functions.However,their potential effects on N₂O emissions are of increasing concern.In this study,an in situ measurement experiment was conducted to investigate the effect of organic fertilizer containing Trichoderma guizhouense(a plant growth-promoting fungus)on soil N₂O emissions from a greenhouse vegetable field.The following four treatments were used:no fertilizer(control),chemical fertilizer(NPK),organic fertilizer derived from cattle manure(O),and organic fertilizer containing T.guizhouense(O+T,referring to bio-organic fertilizer).The abundances of soil N cycling-related functional genes(amoA)from ammonium-oxidizing bacteria(AOB)and archaea(AOA),as well as nirS,nirK,and nosZ,were simultaneously determined using quantitative PCR(qPCR).Compared to the NPK plot,seasonal total N₂O emissions decreased by 11.7%and 18.7%in the O and O+T plots,respectively,which was attributed to lower NH₄⁺-N content and AOB amoA abundance in the O and O+T plots.The nosZ abundance was significantly greater in the O+T plot,whilst the AOB amoA abundance was significantly lower in the O+T plot than in the O plot.Relative to the organic fertilizer,bio-organic fertilizer application tended to decrease N₂O emissions by 7.9%and enhanced vegetable yield,resulting in a significant decrease in yield-scaled N₂O emissions.Overall,the results of this study suggested that,compared to organic and chemical fertilizers,bio-organic fertilizers containing PGPMs could benefit crop yield and mitigate N₂O emissions in vegetable fields.     

Azolla incorporation and dual cropping influences CH4 and N2O emissions from flooded paddy ecosystems

ABSTRACT

To investigate the influence of Azolla (A. filiculoides Lam.) incorporated as a green manure and its subsequent growth as a dual crop with rice on simultaneous methane (CH₄) and nitrous oxide (N₂O) emissions from a flooded alluvial soil planted with rice, a pot experiment with three treatments, chemical fertilizers (NPK) as the control, incorporation of Azolla as green manure (AGM), and AGM plus basal chemical fertilizers (NPK+AGM) was conducted in Tsuruoka, Yamagata, Japan in 2017. AGM and NPK+AGM treatments significantly increased CH₄ emissions at early rice growth stages before 63 days after transplanting (DAT) by 123.0% and 176.7% compared to NPK, respectively. At late rice growth stages (after 63 DAT), only the NPK+AGM treatment significantly increased CH₄ emission by 22.1% compared to NPK. However, percentage of CH₄ emitted after 63 DAT relative to the seasonal CH₄ emission followed the order of NPK (86.2%) > AGM (76.5%) > NPK+AGM (73.3%). Higher CH₄ emissions from AGM and NPK+AGM before 63 DAT were attributed to the incorporated Azolla, while the higher emissions after 63 DAT in all treatment groups were ascribed to rice photosynthesis. AGM and NPK+AGM treatments significantly decreased N₂O emissions by 71.6% and 81.1% compared to NPK, respectively, at early rice growth stages. Azolla incorporation may have restricted N₂O emission from initial soil nitrate before 63 DAT and not have contributed to N₂O emissions after 63 DAT. Significantly higher grain yields were observed under the AGM (32.5%) and NPK+AGM (36.3%) compared to NPK. Together, AGM and NPK+AGM treatments significantly increased seasonal CH₄ emissions by 31.5% and 43.5%, and decreased seasonal N₂O emissions 3.4- and 4.6- fold compared to NPK, respectively. There were no significant differences in the CH₄ emissions per grain yield among the treatments; however compared to NPK, AGM and NPK+AGM treatments significantly reduced N₂O emissions per grain yield by 78.7% and 84.1%, respectively.     

Mechanisms of soil N dynamics following long-term application of organic fertilizers to subtropical rain-fed purple soil in China

In this study, a ¹⁵N tracing incubation experiment and an in situ monitoring study were combined to investigate the effects of different N fertilizer regimes on the mechanisms of soil N dynamics from a long-term repeated N application experiment. The field study was initiated in 2003 under a wheat-maize rotation system in the subtropical rain-fed purple soil region of China. The experiment included six fertilization treatments applied on an equivalent N basis (280 kg N ha⁻¹), except for the residue only treatment which received 112 kg N ha⁻¹: (1) UC, unfertilized control; (2) NPK, mineral fertilizer NPK; (3) OM, pig manure; (4) OM-NPK, pig manure (40% of applied N) with mineral NPK (60% of applied N); (5) RSD, crop straw; (6) RSD-NPK, crop straw (40% of applied N) with mineral NPK (60% of applied N). The results showed that long-term repeated applications of mineral or organic N fertilizer significantly stimulated soil gross N mineralization rates, which was associated with enhanced soil C and N contents following the application of N fertilizer. The crop N offtake and yield were positively correlated with gross mineralization. Gross autotrophic nitrification rates were enhanced by approximately 2.5-fold in the NPK, OM, OM-NPK, and RSD-NPK treatments, and to a lesser extent by RSD application, compared to the UC. A significant positive relationship between gross nitrification rates and cumulative N loss via interflow and runoff indicated that the mechanisms responsible for increasing N loss following long-term applications of N fertilizer were governed by the nitrification dynamics. Organic fertilizers stimulated gross ammonium (NH₄⁺) immobilization rates and caused a strong competition with nitrifiers for NH₄⁺, thus preventing a build-up of nitrate (NO₃⁻). Overall, in this study, we found that partial or complete substitution of NPK fertilizers with organic fertilizers can reduce N losses and maintain high crop production, except for the treatment involving application of RSD alone. Therefore, based on the N transformation dynamics observed in this study, organic fertilizers in combination with mineral fertilizer applications (i.e. OM, OM-NPK, and RSD-NPK treatments) are recommended for crop production in the subtropical rain-fed purple soils in China.     

有机无机肥料配合施用对设施菜田土壤N2O排放的影响

采用静态箱气相色谱法研究了有机无机肥料配合施用对设施菜田土壤N2O排放的影响。结果表明: 1）设施芹菜和番茄施基肥后57 d（灌溉后13 d）出现土壤N2O排放通量峰值,追肥后（施肥与灌溉同步）1 d出现土壤N2O排放通量峰值; 芹菜季和番茄季施用基肥后20 d内N2O排放量分别占当季总排放量的40%65%左右,是土壤N2O主要排放期。2）施用基肥后至定植灌水前各处理土壤N2O排放量逐渐降低,灌水后N2O排放通量迅速上升。各处理土壤N2O排放通量与土壤含水量之间呈显著相关,相关系数在0.43~0.72之间。3）土壤N2O排放主要发生在番茄季,番茄生育期各处理土壤N2O总排放量是芹菜生育期的3.1倍; 各处理土壤N2O排放通量与5 cm土层温度之间总体上呈显著相关,相关系数在0.40~0.58之间。4）设施菜田大幅减施化肥的有机无机肥配合施用模式可显著降低土壤N2O排放量和肥料损失率,芹菜季和番茄季土壤N2O排放量较习惯施肥处理分别降低66.3%和85.1%,肥料损失率分别降低45.2%和74.9%。5）等氮量投入时,施用秸秆较施用猪粪可有效降低土壤N2O排放,芹菜季和番茄季分别降低43.4%和74.2%。