棉花黄枯萎病拮抗菌株的筛选及抗菌蛋白的分离纯化研究

从棉花植株根部筛选的108株芽孢杆菌中,通过对棉花黄枯萎主要病原菌大丽轮枝菌拮抗效果的复筛,分离到一株LC-105菌株,其抑菌圈达到20 mm以上;本研究从该菌株的发酵代谢产物中,分离出活性拮抗物质,进一步通过DEAE-Sephadex A-50离子交换层析和Sephacryl-S100凝胶层析对其代谢产物进行了分离纯化,证明该拮抗物质为一种蛋白质,最后通过聚丙烯酰胺凝胶电泳证实了所分离物质的纯度达到电泳纯.     

Study on the Projects of the Daily Regulation of Jinghong Hydroelectric Station on Lancang River

The one dimension mathematical model has been adopted to study the projects of the daily regulation of hydroelectric station: the implicit format of Preissmann has been adopted to solve the equation.The program of one dimension unsteady flow has been worked out.The result of calculation of the program has been verified by the result of the physical model and the coincide well.Under 4 kinds of projects of the daily regulation of Jinghong Hydroelectric Station,the unsteady flow conditions in lower reaches of the dam with the length of about 85km have been calculated by the program.The graph has been adopted to analyze the important characteristic parameters of the unstable flow conditions of 4 kinds of projects: the range of water level per day,the range of water level per hours and the range of water level per 20 minutes.Finally,the project of the daily regulation of Jinghong Hydroelectric Station which has a smallest influence on the navigable flow conditions has been obtained.     

Design and Analysis of the Air Flow and Distribution of Laminated Air Conditioning in Summer in the Powerhouse of Sanxia Hydropower Station

Around the design of air flow and distribution about the laminated air conditioning in summer in the powerhouse of Sanxia hydropower station, the system division, air flow pattern, air supply state and volume etc have been analyzed separately. Based on the existing domestic achievements, the relative calculation model has been established and the application method has been explored. From this the program named as AFDC/LACS has been compiled with language FORTRAN77 and the calculation on the airflow and distribution of the place mentioned above has been done with several design conditions. Thus some batches of the technical parameters have been provided to plan the test conditions in the heat simulated test..     

Forming process of asymmetric wall forging

Asymmetric wall products are widely used in the engineering. However, there are many limitations in the traditional forming processes employed to produce them. Oil pump stator has been studied as an instance to present the forming process of asymmetric wall products. First, a theoretical analysis of the forging's forming process has been made. Second, a rigid-visco-plastic finite element model of oil pump stator has been constructed and three dimensions FEM simulation and analyzing of extruding process have been taken by employing the simulation software. At last, parameters of the forming process have been optimized, the rule of forging's metal flow in the forming process has been analyzed and some distributions of stress and strain have been offered. These data can be use to direct the production of the other asymmetric wall forgings.     

Optimal Design of Aerostatic Bearing

A method using fuzzy sets theory and optimization method in designing aerostatic bearings has been presented,which has solved the multiobjective optimal designing problems of aerostatic bearings and has obtained rational structure parameters,raised the efficiency of designing.The automation of aerostatic bearing design has been realized.     

食品中亚硝胺类化合物的危害及控制研究进展

亚硝胺是对人类健康危害性极高的化合物之一。本文阐述N-亚硝基化合物的危害及在食品中的分布状况,综述应用辐照和天然植物提取物等阻断剂消除和控制食品中N-亚硝基化合物技术措施的研究进展。     

现代艺术在城市园林设计中运用的讨论

摘要：随着当今社会的发展,大众的生活水平、文化水平、对艺术的认识水平不断提高,人们的审美水平在日益的增长。现代艺术设计是新时代的产物,而设计与艺术是无法分隔开的。城市园林设计是现代最为综合的艺术,它与现代艺术形式之间有着必然的联系。如今,城市园林设计飞速发展,艺术设计在艺术学领域也在蓬勃兴起。本文简要对城市园林设计的内涵做了界定,阐述了城市园林设计与现代艺术之间的关系,从园林中的小品、雕塑、植物造景等多个方面,分析了现代艺术影响下的城市园林设计。     

一流课程背景下“食品营养学”案例库的建设及评价

"食品营养学"课程属性和行业需求与一流课程建设"两性一度"指导思想三者高度统一。初步形成以学生为中心、产出为导向,强化学生实践技能培养的"食品营养学"一流课程建设的总体思路,构建了"食品营养学"案例库并成功实施于教学中,跟踪最近3年的教学效果,对比发现学生的创新意识和实践能力得到较大提高。     

Dynamic Optimization of System Parameters For the Epicyclic Gear Drive with Internal Tooth

The epicyclic gear drive with internal tooth has been analysed,the formula has been also deduced for computing the inertial force and change of contact force,and the dynamic optimization of system parameters for the force of bearing has been carried,it has given a reliable proof to reduce the dynamic load and prolong the life of bearing of three ring type reducer.     

Discussion of the Stiffness Reduction Factor of Bent-columns

The simplified structural analysis and design method employing stiffness reduction of member bars,has been accepted by Chinese Standard, in which second-order effects already been considered through elastic finite element analysis,and its accuracy mostly depends on the rationality of the stiffness reduction factor.Due to the absence of investigation in the stiffness reduction factor of bent frame,according to the non-linear finite element analysis of several single-story single-span bent frames,the stiffness reduction factor of bent-columns has been suggested.And the rationality of this factor has been proved by comparison of the internal force in bent-columns and displacement of bent frames in second-order elasticity analysis and non-linear finite element analysis.     

离子束生物技术在药用植物遗传改良中的应用前景

本文主要简要介绍了离子束生物工程的特点,系统阐述了药用植物资源领域的现状以及离子束作为一种新的诱变源在药用植物遗传改良中的应用前景。     

GIS- and RS-based Analysis of Suburban Landscape Layout in Haikou City

Landsat TM/ETM+ and Alos remote sensing data of the study area in 1991, 2001 and 2010 were interpreted by using object-oriented RS image extraction technology, landscape ecology theories were applied as well as GIS and FRAGSTATS4.1 to choose reasonable landscape indexes for studying temporal-spatial changes of suburban landscape layouts of Haikou City, exploring spatial features and change tendency of rural landscapes. According to the results, tropical garden landscapes have taken a dominant status in the last 2 decades; tropical farmland landscapes have had the most complicate shapes; tropical open forest landscapes have been most influenced by human interventions but had the best connectivity and high degree of concentration; tropical settlement landscapes and tropical meadow landscapes have been widely scattered with poor connectivity; landscape connectivity has been well maintained; contagion index has changed slightly, landscape equilibrium has been stable; landscape diversity has been maintained, all landscape types have been evenly distributed, landscape fragmentation has increased, and landscape heterogeneity has grown.     

Stability Analysis for the Excavation Engineering Under Coupling Effect of Seepage from Heavy Rain and River Fluctuation

The seepage accident is very easily occurred, when the excavation engineering has been constructed in rainy season and it is very easily influenced by the coupling effect of seepage duo to heavy rain and water level fluctuation of neighboring river. So a coupling seepage equation that based on the theory of Dupuit has been established for the ground water in the layered foundation. And then, the water table surrounding the excavation engineering has been determined on the consideration of the coupling effect of seepage duo to heavy rain and water level fluctuation of river. In the meantime, by means of the theory of coupling analysis of stress and seepage and the software of Midas, a model of coupling analysis of stress and seepage has been built for the specific excavation engineering and the seepage destroy characteristics of this project has been researched.     

Control of ripening in transgenic tomatoes

Major progress has been made over the last few years in the identification and regulation of tomato ripening genes. At least 25 genes showing elevated expression during ripening have been cloned and several, including polygalacturonase, which modifies fruit textures, have been shown to be ripening-specific. In addition, genes have been cloned for ACC synthase and ACC oxidase, which control the synthesis of ethylene, which plays a critical role in ripening. Inhibition of expression of polygalacturonase, pectinesterase, ACC synthase, ACC oxidase and phytoene synthase has been achieved in transgenic plants, using antisense technology. The expression of several genes has also been inhibited by sense gene suppression. New traits caused by these transgenes are stably inherited. Antisense tomatoes with reduced polygalacturonase have improved textural qualities which are being exploited commercially for the fresh and processed markets. Overexpression of phytoene synthase has been shown to restore carotenoid production in the yellow flesh mutant and can be used to enhance colour in other cultivars. Antisense fruit in which ACC synthase or ACC oxidase are inhibited show slower ripening and reduced over-ripening. ACC oxidase antisense genes have also been shown to delay leaf senescence. It is to be expected that further genes determining other quality traits will be identified and manipulated soon.     

粮食作物空间诱变育种技术工作研究进展

1987年以来,先后成功的利用返回式卫星（15次）和飞船（6次）进行生物搭载试验,选育出一批优良新品种、新品系,其中通过省级以上审定的30多个,并在各地推广应用,取得明显的经济效益和社会效益,同时,在空间诱变育种机理研究上也取得重要进展。各地实践证明,空间诱变育种是集太空育种技术与农业育种技术相结合的高技术育种新途径,具有明显加快育种进程,缩短育种周期,提高育种效益的作用     

Calculation of Fatigue Reliable Life of Natural Gas Pipeline

Various defects and cracks may be produced in the welding process in natural gas pipeline.Under variable pressure of natural gas,the cracks will be propagated it may lead to break and invalidity.The effect of internal surface cracks are more serious.It has been researched that the propagation along radial direction of internel surface cracks in the natural gas pipeline.The fatig life of pipeline has been calculated.On the other hand,considering the size of cracks, pressure of gas and character of material are random variable,and by using reliability method,the fatig reliable life of pipeline has been determined.This method has some practical value in design, working and overhaul of pipeline.     

超高茬麦套稻的产量及低碳效应分析

通过对超高茬麦田套稻稻作方式的机理进行了介绍,分析了其低碳优势和高产原因,为现代农业生产提供参考。     

Study of Larger Taper Cutting Mechanism for Wire-EDM Machine

After a analyze for current taper cutting mechanism of NC wire-EDM machine,the paper but forward a new mechanism for larger taper cutting and introducted the working principle in great detail. It has been proved the new mechanism could solve many key techanical problems which current mechanisms had. The new mechanism has been patented. Newly developed NC wire-EDM,DK7725 TC,has been warmly welcome by numerous users.     

冬枣采后浆胞病定性研究

对冬枣采后贮藏期间危害最为严重的病害--浆胞病进行了定性研究,通过对其病原菌的分离、培养和回接试验,确定了冬枣浆胞病为混合感染,病原菌种类分别为交链孢霉菌和根菌索菌两种;同时调查不同温度对冬枣浆胞病病原菌生长的影响.     

牛奶体细胞快速检测方法研究进展

体细胞数是评价牛奶品质的重要指标,同时也是奶牛乳房炎诊断的一个重要指标。在乳业发达国家的牧场,奶站和乳品加工企业都将体细胞检测作为一项日常工作,并且对体细胞检测技术也做了大量的研究工作。主要综述了国内外的体细胞快速检测技术和研究进展。