Agronomic Assessment of Nitrogen Use Efficiency in Spring Wheat and Interrelations with Leaf Greenness Under Field Conditions

Four spring wheat genotypes (Triticum aestivum L.) were grown without (N0 = 0 kg N ha^?1) and under ample (N1 = 250 kg ha^?1) nitrogen (N) fertilizer in field experiments in two seasons. The aim was to assess genotypic variation in N use efficiency (NUE) components and N-related indices during grain filling thus to identify superior wheat genotypes. Leaf chlorophyll (SPAD) readings at crucial growth stages were employed to help differentiate genotypes. Interrelations between yield and N-related indices with SPAD, where also assessed to explain possible pathways of improving NUE early in the growing season. Results showed that genotypic effects on NUE were mostly evident in 2000, a year with drier preanthesis and wetter postanthesis than the normal periods. ‘Toronit’ almost always had the highest biomass yield (BY) and grain yield (GY). Except in 1999 under N0, ‘L94491? showed the highest % grain N concentration (GNC). Genotypes affected SPAD at almost all stages and N fertilization delayed leaf senescence for all genotypes and growth seasons. Correlations between SPAD at different growth stages and GY, N biomass yield at maturity (NBYM) and GNC were significant (P≤ 0.001), positive and strong/very strong (>r = 0.7). N translocation efficiency (NTE) was inversely related to PANU (~r = ? 0.77, P≤ 0.001), suggesting that N after anthesis is being preferentially transported to the ears to meet the N demand of the growing grains. It is concluded that there is still a large potential for increased NUE by improved N recirculation, use of fast and inexpensive crop N monitoring tools and high yielding, N uptake efficient genotypes.

Abbreviations: NUE, Nitrogen use efficiency; SPAD, Minolta SPAD-502 chlorophyll meter, NHI, nitrogen harvest index; HI, Harvest index; NTE, N translocation efficiency from vegetative plant parts to grain; DMTE, dry matter translocation efficiency; CPAY, contribution of pre-anthesis assimilates to yield; PANU, Post-anthesis N uptake, d.a.s., days after sowing, N0, zero (0) kg ha^?1 applied N fertilizer, N1, 250 kg ha^?1 applied N fertilizer.     

COURSE OF DRY MATTER AND NITROGEN ACCUMULATION OF SPRING WHEAT GENOTYPES KNOWN TO VARY IN PARAMETERS OF NITROGEN USE EFFICIENCY

Field experiments were conducted for two years to compare and identify bread spring wheat (Triticum aestivum L.) genotypes which make the most efficient use of nitrogen (N). Such information is required for breeding strategies to reverse the negative relationship between yield and protein content. Three Swiss spring wheat cultivars (‘Albis’, ‘Toronit’, ‘Pizol’) and an experimental line (‘L94491’) were grown without (N0; 0 kg N ha^?1) and with high fertilizer N [(NH₄NO₃); (N1; 250 kg N ha^?1) supply on a clay loam soil with low organic matter content. Biomass and nitrogen accumulation in biomass as well as the leaf growth and senescence patterns (SPAD) were investigated in an attempt to explain the physiology of growth and N translocation of these genotypes. The pre-anthesis accumulation of biomass and N in the biomass depended on genotype only at N1 in 2000. In this year, conditions were less favorable for the pre-anthesis accumulation of biomass and N, which was, on average, 10 and 20% lower, respectively, of the total than in 1999. The contribution of pre-anthesis assimilates to the grain yield (CPAY) was higher in 1999 for all genotypes (36.9%) compared to 2000 (13.5%) except ‘Toronit’. Between anthesis and maturity the climate influenced the genetic variability of some N use efficiency components: N translocation efficiency (NTE) and dry matter translocation efficiency (DMTE). NTE was higher in 1999 (68.1%) compared to 2000 (50.7%); 1999 was a year in which the post-anthesis period was drier and warmer than usual. ‘Toronit’ produced the highest biomass by maturity due mainly to greater and longer lasting green leaf area after anthesis. ‘Albis’ performed relatively well under low input conditions, with considerable amounts of N being re-translocated to the seeds at maturity (NHI), whereas ‘Pizol’ accumulated in grains N as high as for ‘L94491’. In a humid temperate climate breeding for greater N uptake and partitioning efficiency may be a promising way to minimize N losses and produce high phytomass and grain yields. Using high protein lines as selection material and combining them with high biomass genotypes may lead to high protein contents without decreasing yield.     

SUBSOIL ROOT GROWTH OF FIELD GROWN SPRING WHEAT GENOTYPES (TRITICUM AESTIVUM L.) DIFFERING IN NITROGEN USE EFFICIENCY PARAMETERS

In a two-year (1999–2000) field experiment four Swiss spring wheat (Triticum aestivum L.) genotypes (cvs. ‘Albis’, ‘Toronit’ and ‘Pizol’ and an experimental line ‘L94491’) were compared for genotypic differences in the root parameters that determine uptake potential and nitrogen use efficiency (NUE):root surface area (RSA) and its components, root length density (RLD) and the diameter of the roots. The genotypes were grown under no (N0) and under ample fertilizer nitrogen (N) [ammonium nitrate (NH₄NO₃); N1; 250 kg N ha^?1] supply. Root samples were taken from all the genotypes at anthesis from the subsoil (30–60 cm). Genotypic effects on RLD and RSA were evident only in 2000 and large amounts of N fertilizer usually diminished root growth. Adequate soil moisture in 1999 may have favored the establishment of the root system of all the genotypes before anthesis. Parameters of NUE for each genotype were also determined at anthesis and at physiological maturity. ‘Albis’ the least efficient cv. in recovering fertilizer N (ranged from 36.5 to 61.1%) with the lowest N uptake efficiency (0.47 to 0.79 kg kg^?1) had the lowest RLD and RSA in both seasons. Among genotypes ‘Toronit’, a high-yielding cv., efficient in recovering fertilizer N, exhibited the higher NUE (22.4 to 29.3 kg kg^?1) and tended to have the highest values of RLD and RSA. Nitrogen fertilization also led to an increase in the proportion of roots with diameters less than 300 μm and decreased the proportion of roots with diameters of 300 to 700 μm. These trends were more pronounced for cv. ‘Pizol’ in 1999 and for cv. ‘Toronit’ in 1999 and 2000. By anthesis in a humid temperate climate, there are no marked differences in the subsoil root growth of the examined genotypes. Some peculiarities on the root growth characteristics of the cultivars ‘Albis’ and ‘Toronit’ may partially explain their different NUE performance.     

Nitrogen and genotype effects on root growth and root survivorship of spring wheat

The nitrogen (N) fertilization of wheat (Triticum aestivum L.) is important for stable and high grain yield. However, the effect of N on root growth and survivorship is poorly understood. The objectives of this study were (1) to determine the effect of varying N availability on the growth and survivorship of roots and (2) to determine whether genotypic variation in N‐related traits are linked to root growth and survivorship. In a two‐year study, two spring wheat cultivars (Albis and Toronit) and an experimental line (L94491) were grown under low (20 kg N ha^–1) and high N supply (270 kg N ha^–1) in lysimeters equipped with minirhizotrons. The genotypes showed significant differences in N‐related traits: total shoot N content, grain N yield, N harvest index, and rate of decline in flag‐leaf greenness. However, there were relatively weak and inconsistent genotypic effects on the time course of root density, root growth during grain filling, and root survivorship. The level of N supply was the factor that most influenced the establishment, growth, and survivorship of roots; the high N supply, depending on the year and genotype, increased growth and survivorship of roots from 0% to 68% and 24% to 34%, respectively.     

Genotypic Differences in Dry Bean Yield and Yield Components as Influenced by Nitrogen Fertilization and Rhizobia

Dry bean (Phaseolus vulgaris L.) is an important legume worldwide and nitrogen (N) is most yield limiting nutrients. A field experiment was conducted for two consecutive years to evaluate response of 15 dry bean genotypes to nitrogen and rhizobial inoculation. The N and rhizobia treatments were (i) control (0 kg N ha^?1), (ii) seed inoculation with rhizobia strains, (iii) seed inoculation with rhizobia strains + 50 kg N ha^?1, and (iv) 120 kg N ha^?1. Straw yield, grain yield, and yield components were significantly influenced by N and rhizobial treatments. Grain yield, straw yield, number of pods m^?2, and grain harvest index were significantly influenced by year, nitrogen + rhizobium, and genotype treatments. Year × Nitrogen + rhizobium × genotype interactions were also significant for these traits. Hence, these traits varied among genotypes with the variation in year and nitrogen + rhizobium treatments. Inoculation with rhizobium alone did not produce maximum yield and fertilizer N is required in combination with inoculation. Based on grain yield efficiency index, genotypes were classified as efficient, moderately efficient, and inefficient in nitrogen use efficiency (NUE). NUE defined as grain produced per unit N applied decreased with increasing N rate. Overall, NUE was 23.17 kg grain yield kg^?1 N applied at 50 kg N ha^?1 and 13.33 kg grain per kg N applied at 120 kg N ha^?1.     

Timing and Method of 15Nitrogen-Labeled Fertilizer Application on Grain Protein and Nitrogen Use Efficiency of Spring Wheat

ABSTRACT

Grain protein content is one of the most important quality constraints for bread wheat (Triticum aestivum L.) production in eastern Canada. A field experiment was conducted for two years (1999 and 2000) on the Central Experimental Farm, Ottawa, Canada, to study whether split application of nitrogen (N) fertilizer improved grain protein content and nitrogen-use efficiency (NUE). Two cultivars (‘Celtic,’ as N-responsive and ‘Grandin’, as N-non-responsive) were grown using three different N doses and application methods: (1) 100 kg N ha^?1 as NH₄NO₃, soil-applied at seeding with ¹⁵N₂-labeled NH₄NO₃ to microplots, (2) 60 kg N ha^?1 soil-applied at seeding plus 40 kg N ha^?1 foliar-applied at the boot stage with ¹⁵N₂-labeled urea to microplots, and (3) 90 kg N ha^?1 as soil-applied at seeding plus 10 kg N ha^?1 foliar-applied at the boot stage with ¹⁵N₂-labeled urea to microplots. Plants were sampled at heading and maturity. While dry-matter production and grain yields were not affected by the treatments in either year, N application methods influenced tissue N concentration and NUE. In 1999, extended drought stress led to significant yield reduction; in 2000, foliar application of 10 kg N ha^?1 at the boot stage significantly increased grain N concentration when grain protein was under the limit for bread quality, suggesting that later-applied N can contribute to grain protein content. At maturity, the average NUE was 22.3% in 1999 and 34.5% in 2000, but was always greater when all N was applied at seeding (42.5%) than when N was foliar-applied at the boot stage (18.5% to 24.5%). We conclude that application of a small amount of fertilizer N at the boot stage can improve the bread-making quality of spring wheat by increasing grain protein concentration.     

Field-level comparison of nitrogen rates and application methods on maize yield,grain quality and nitrogen use efficiency in a humid environment

Appropriate nitrogen (N) management practices are of critical importance in improving N use efficiency (NUE), maize (Zea mays) yield and environmental quality. A six-year (2005–2010) on-farm trial was conducted in Ottawa, Canada to assess the effects of N rates and application methods on grain yield and NUE. In four out of the six-year study, grain yield increased by 60–77 kg ha^?1 by sidedress, compared to 49–66 kg ha^?1 for each kg N ha^?1 applied at preplant. Grain yield response to N between the two strategies was similar in the other growing seasons. Sidedress strategy required 15 kg N ha^?1 less of the maximum economic rate of N (MERN) than preplant application. Our results indicate that sidedress application of 90–120 kg N ha^?1 with a starter of 30 kg N ha^?1 resulted in greater yield, grain quality and NUE than preplant N application in this cool, humid and short growing-season region.     

Effect of nitrogen rate on plant nitrogen loss in winter wheat varieties 1

Gaseous nitrogen (N) loss from winter wheat (Triticum aestivum L.) plants has been identified, but has not been simultaneously evaluated for several genotypes grown under different N fertility. Two field experiments were initiated in 1993 and 1994 at the Agronomy Research Station in Stillwater and Perkins to estimate plant N loss from several cultivars as a function of N applied and to characterize nitrogen use efficiency (NUE). A total of five cultivars were evaluated at preplant N rates ranging from 30 to 180 kg·ha^‐1. Nitrogen loss was estimated as the difference between total forage N accumulated at anthesis and the total (grain + straw) N at harvest. Forage, grain, straw yield, N uptake, and N loss increased with increasing N applied at both Stillwater and Perkins. Significant differences were observed among varieties for yield, N uptake, N loss, and components of NUE in forage, grain, straw, and grain + straw. Estimates of N loss over this two‐year period ranged from 4.0 to 27.9 kg·ha^‐1 (7.7 to 59.4% of total forage N at anthesis). Most N losses occurred between anthesis and 14 days post‐anthesis. Avoiding excess N application would reduce N loss and increase NUE in winter wheat varieties. Varieties with high harvest index (grain yield/total biomass) and low forage yield had low plant N loss. Estimates of plant loss suggest N balance studies should consider this variable before assuming that unaccounted N was lost to leaching and denitrification.     

Response of Wheat Cultivars to Nitrogen and Sulfur for Crop Yield,Nitrogen Use Efficiency,and Protein Quality in the Semiarid Region

ABSTRACT

The effects of nitrogen (N at 0, 100 and 180 kg N ha-1) and sulfur (S at 0, 20, 40 and 60 kg S ha-1) on crop yield, nutrient uptake, nitrogen use efficiency (NUE), and amino acid composition of two bread wheat cultivars, ‘Shehan’ and ‘Enkoy,’ grown in Andisols and Cambisols in randomized blocks with three replications were evaluated. Both cultivars responded significantly (P < 0.05) to N and S applications and S application with N improved the NUE by 28%. The yield increase for the two cultivars by N and S application ranged between 0.8 to 2.4 Mg ha^?1. The N concentration increased significantly from N0 to N2 in both cultivars. Sulfur fertilization increased the concentration of cysteine and methionine by 27% and 14%, respectively, as compared to N alone. The grain yield, NUE, N, and S uptake, and the S-amino acid concentration of ‘Enkoy’ were significantly higher than ‘Shehan’ cultivar.     

Nitrogen Balance in the Magruder Plots Following 109 Years in Continuous Winter Wheat

Abstract

The Magruder plots are the oldest continuous soil fertility wheat research plots in the Great Plains region, and are one of the oldest continuous soil fertility wheat plots in the world. They were initiated in 1892 by Alexander C. Magruder who was interested in the productivity of native prairie soils when sown continuously to winter wheat. This study reports on a simple estimate of nitrogen (N) balance in the Magruder plots, accounting for N applied, N removed in the grain, plant N loss, denitrification, non‐symbiotic N fixation, nitrate (NO₃ ^?) leaching, N applied in the rainfall, estimated total soil N (0–30 cm) at the beginning of the experiment and that measured in 2001. In the Manure plots, total soil N decreased from 6890 kg N ha^?1 in the surface 0–30 cm in 1892, to 3198 kg N ha^?1 in 2002. In the Check plots (no nutrients applied for 109 years) only 2411 kg N ha^?1 or 35% of the original total soil organic N remains. Nitrogen removed in the grain averaged 38.4 kg N ha^?1 yr^?1 and N additions (manure, N in rainfall, N via symbiotic N fixation) averaged 44.5 kg N ha^?1 yr^?1 in the Manure plots. Following 109 years, unaccounted N ranged from 229 to 1395 kg N ha^?1. On a by year basis, this would translate into 2–13 kg N ha^?1 yr^?1 that were unaccounted for, increasing with increased N application. For the Manure plots, the estimate of nitrogen use efficiency (NUE) (N removed in the grain, minus N removed in the grain of the Check plots, divided by the rate of N applied) was 32.8%, similar to the 33% NUE for world cereal production reported in 1999.     

N-Source Effects on Temporal Distribution of NO3-N Leaching Losses to Subsurface Drainage Water

Understanding the temporal distribution of NO₃-N leaching losses from subsurface drained ‘tile’ fields as a function of climate and management practices can help develop strategies for its mitigation. A field study was conducted from 1999 through 2003 to investigate effects of the most vulnerable application of pig manure (fall application and chisel plow), safe application of pig manure (spring application and no-tillage) and common application of artificial nitrogen (UAN spring application and chisel plow) on NO₃-N leaching losses to subsurface drainage water beneath corn (Zea mays L.)–soybean (Glycine max L.) rotation systems as a randomized complete block design. The N application rates averaged over five years ranged from 166 kg-N ha^?1 for spring applied manure to 170 kg-N ha^?1 for UAN and 172 kg-N ha^?1 for fall applied manure. Tillage and nitrogen source effects on tile flow and NO₃-N leaching losses were not significant (P?<?0.05). Fall applied manure with CP resulted in significantly greater corn grain yield (10.8 vs 10.4 Mg ha^?1) compared with the spring manure-NT system. Corn plots with the spring applied manure-NT system gave relatively lower flow weighted NO₃-N concentration of 13.2 mg l^?1 in comparison to corn plots with fall manure-CP (21.6 mg l^?1) and UAN-CP systems (15.9 mg l^?1). Averaged across five years, about 60% of tile flow and NO₃-N leaching losses exited the fields during March through May. Growing season precipitation and cycles of wet and dry years primarily controlled NO₃-N leaching losses from tile drained fields. These results suggest that spring applied manure has potential to reduce NO₃-N concentrations in subsurface drainage water and also strategies need to be developed to reduce early spring NO₃-N leaching losses.     

Residue,tillage and N-fertilizer rate affected yield and N efficiency in irrigated spring wheat

Yield and nitrogen (N)-content in wheat was studied under applied treatments of crop residues (legume vs. cereal), tillage depths (deep vs. shallow) and N-fertilizer rates (0, 40, 80, 120 and 160 kg ha^?1) at wheat-maize cropping systems. Experiments were conducted at Agronomy Research farm, the University of Agriculture, Peshawar Pakistan, during winter season 2009–2010 and 2010–2011 crop growth seasons. Well-chopped crop residues (5 t ha^?1) on dry matter basis of legume (Vigna unguicuata) and cereal (Zea mays) were applied to soil and subsequently plowed with mold-board plow as deep tillage (DT) and cultivator as shallow tillage (ST) treatment (main plot treatments). A month after residue and tillage application, seedbed was prepared and wheat was planted with drill in rows 25 cm apart in middle of November each year. Phosphorus and potassium were applied uniformly 80 and 40 kg ha^?1, respectively during seedbed preparation. N-fertilizer rates were applied in two splits: half 15 days after sowing (DAS) and other half 45 DAS (sub-plot treatment). Uniform cultural practices were applied during crop growth and development. Legumes residues amendments showed better responses than cereal but lower than no-residue treatment for N-content in leaf blade before anthesis (LBA), after anthesis (LAA), straw N-content (SNC), grain N-content (GNC), grain N-uptake (GNU), crop N-removal (CNR), recovery efficiency of added nitrogen (REAN), N-use efficiency (NUE), grain N-uptake (GNU) and grain yield. Likewise, shallow tillage proved better than deep tillage system for LBA, LAA, SNC, GNC, GNU, CNR, REAN, NUE, GNU and grain yield. Increased N-fertilizer from control onwards showed significant (p > 0.05) increments in LBA, LAA, SNC, GNC, GNU, CNR, N-uptake and grain yield. Treatments interaction was also found significant (p > 0.05). Study suggested, regardless of the given treatments, GNU and grain yield were in strong positive linear relationship. Legume residue incorporated shallow out yielded GNU and NUE of spring wheat in wheat-maize cropping system. It is concluded that LR and ST with 120 kg N ha^?1 ensures production of good wheat quantity and quality.     

Enhancing Environmental Performance with Winter Cover Cropping after Potato Harvest in Eastern Canada

ABSTRACT

In humid climates, the risk of nitrate leaching and topsoil loss due to erosion is high on bare soil in the fall after potato (Solanum tuberosum L.) harvest and in the spring with snowmelt. This 2-year study (2016–2017) compared three winter cover crops. Two of these are used as cash crops (winter rye [Secale cereale L.], winter wheat [Triticum aestivum L.]), and one is a winter-killed cover crop (spring barley, Hordeum vulgare L.). They were all seeded on two dates after potato harvest (end of September or first week of October) in Prince Edward Island, Canada. The measured parameters included soil nitrate measured at different times in fall and in the following spring and summer, splash detachment, C and N contents in splashed sediments, cereal straw dry matter yield, and cereal grain yield. In both years, all winter cover crops decreased splash detachment compared with the no winter cover control, with winter rye having the greatest reduction. A similar trend was observed for C and N contents in splashed sediments. There was a trend toward lower soil nitrate following winter cover crops in comparison with bare soil, but the trend was not consistent across trials and sampling dates. Winter wheat grain yield ranged from 4.5 to 7.6 Mg ha^?1, while that associated with winter rye ranged from 3.2 to 5.1 Mg ha^?1. Therefore, winter cereal seeded after potato harvest can constitute a good source of revenue while mitigating the risk of soil erosion and reducing nitrate leaching in some cases.     

NITROGEN NUTRITION ON LEAF CHLOROPHYLL,CANOPY REFLECTANCE,GRAIN PROTEIN AND GRAIN YIELD OF WHEAT VARIETIES WITH CONTRASTING GRAIN PROTEIN CONCENTRATION

Wheat cultivars (‘AC Barrie’, ‘Brook Field’, ‘Hoffman’, and ‘Norwell’) with different protein concentrations were compared under four nitrogen (N) levels (0, 50, 100 and 150 kg ha^?1) in an environment-controlled greenhouse, and the same experiment with an additional N level (200 kg N ha^?1) was repeated in the field in 2007. In the greenhouse experiment, application of 100 kg N ha^?1 resulted in significantly greater grain yield due mainly to higher number of grains per spike and heavier mean grain weight; in the field study, the 150 kg N ha^?1 treatment produced the greatest yield (P<0.01) primarily due to more number of grains per spike. Crude grain protein percentage was increased significantly with each increment of N up to the highest level; however, protein yield (kg ha^?1) increased significantly with fertilizer up to 150 kg N ha^?1. Leaf chlorophyll contents were increased linearly with increment of N levels up to 150 kg ha^?1 both in the greenhouse and field trials while leaf area indices continued to increase up to the highest application rate (200 kg N ha^?1). Canopy reflectance, expressed as normalized difference vegetation index (NDVI), attained maximum value with 150 kg N ha^?1 in the field experiment. Among the varieties tested, “Hoffman” out-yielded other three varieties due to heavier grain weight. Although highest grain and/or plant crude protein content were recorded in ‘AC Barrie’, it was the variety ‘Hoffman’ that produced the highest total protein (kg ha^?1) with largest NDVI and leaf area index (LAI) values.     

The Relationship Between Yield and Fructan Exo-Hydrolases Activity in Two Drought Resistant Wheat Cultivars Grown Under Different Fertilizer and Tillage Treatments

Using two drought resistant wheat (Triticum aestivum L.) cultivars, ‘Changwu134’ and ‘Changhan58,’ a field experiment was conducted in ChangWu Agro-ecological Experiment Station on China's Loess Plateau during 2008 and 2009 to compare the effects of different fertilizer and tillage treatments on the fructan contents as well as fructan exo-hydrolase (FEH) activity in relation to wheat yield. We found that ‘Changhan58’ had greater yield and fructan content in the penultimate internode and higher FEH activity than did ‘Changwu134.’ For ‘Changhan58.’ applying 195 kg·ha^?1(120 +75) N plus 45000 kg·ha^?1 of pig manure and 120 kg·ha^?1 phosphate under conservation tillage produced the highest yield (6769 ka/ha), fructan content in penultimate internode, water use efficiency (WUE), as well as FEH activity among the fertilizer and tillage treatments. Therefore, routine soil management for wheat should focus on combined use of manures and inorganic fertilizer to enhance the amount and transportation efficiency of WSC and ultimately ensure greater yield.     

Winter Wheat Yield,Quality, and Nitrogen Removal Following Compost- or Manure-Fertilized Sugarbeet

To efficiently use nitrogen (N) while protecting water quality, one must know how a second-year crop, without further N fertilization, responds in years following a manure application. In an Idaho field study of winter wheat (Triticum aestivum L.) following organically fertilized sugarbeet (Beta vulgaris L.), we determined the residual (second-year) effects of fall-applied solid dairy manure, either stockpiled or composted, on wheat yield, biomass N, protein, and grain N removal. Along with a no-N control and urea (202 kg N ha^?1), first-year treatments included compost (218 and 435 kg estimated available N ha^?1) and manure (140 and 280 kg available N ha^?1). All materials were incorporated into a Greenleaf silt loam (Xeric Calciargid) at Parma in fall 2002 and 2003 prior to planting first-year sugarbeet. Second-year wheat grain yield was similar among urea and organic N sources that applied optimal amounts of plant-available N to the preceding year’s sugarbeet, thus revealing no measurable second-year advantage for organic over conventional N sources. Both organic amendments applied at high rates to the preceding year’s sugarbeet produced greater wheat yields (compost in 2004 and manure in 2005) than urea applied at optimal N rates. On average, second-year wheat biomass took up 49% of the inorganic N remaining in organically fertilized soil after sugarbeet harvest. Applying compost or manure at greater than optimum rates for sugarbeet may increase second-year wheat yield but increase N losses as well.

Abbreviations CNS, carbon–nitrogen–sulfur     

Variation in Yield,Phosphorus Uptake,and Physiological Efficiency of Wheat Genotypes at Adequate and Stress Phosphorus Levels in Soil

Genetic differences among crop genotypes can be exploited for identification of genotypes more suited to a low‐input agricultural system. Twenty wheat (Triticum aestivum L.) genotypes were evaluated for their differential yield response, phosphorus (P) uptake in grain and straw, and P‐use efficiency at the zero‐P control and 52 kg P ha^?1 rates. Substantial and significant differences were obvious among genotypes for both grain and straw yields at stress (8 mg P kg^?1 soil, native soil P, no P addition) and adequate (52 kg P ha^?1) P levels. Genotype 5039 produced maximum grain yield at both P levels. Relative reduction in grain yield due to P‐deficiency stress [i.e., P stress factor (PSF)] ranged between none and 32.4%, indicating differential P requirement of these genotypes. Pasban 90, Pitic 62, Rohtas 90, Punjab 85, and line 4943 did not respond to P application and exhibited high relative yield compared to those at adequate P level. FSD 83 exhibited the best response to P with maximum value for PSF (32.4%). Genotypes were distributed into nine groups on the basis of relationship between grain yield and total P uptake. Rohtas 90 and lines 4072 and 5039 exhibited high grain yield and medium P uptake (HGY‐MP). However, line 5039 with high total index score utilized less P (12.2 kg P ha^?1) than line 4072 and Rohtas 90 (13.5 and 13.6 kg P ha^?1, respectively). Moreover, this genotype also had greater P harvest index (PHI, %) and P physiological efficiency index (PPEI) at stress P level. Pasban 90, Pitic 62, and Pak 81 had the greatest total index score (21), mainly due to high total P uptake, but yielded less grain than lines 5039 and 4072 under low available P conditions. Line 6142 had minimum total index score (15) and also produced minimum grain yield. A wide range of significant differences in PPEI (211 to 365 kg grain kg^?1 P absorbed at stress and 206 to 325 kg grain kg^?1 P absorbed by aboveground plant material at adequate P) indicated differential utilization of absorbed P by these genotypes for grain production at both P levels. It is concluded from the results that wheat genotypes differed considerably in terms of their P requirements for growth and response to P application. The findings suggest that PSF, PHI, and PPEI parameters could be useful to determine P‐deficiency stress tolerance in wheat.     

Effects of Irrigation Water Salinity and Leaching Fraction on Yield and Evapotranspiration in Spring Wheat

To determine the effects of irrigation water salinity and leaching fraction on crop evapotranspiration (ETc), grain yield, straw yield, shoot sodium (Na), and chloride (Cl) concentrations of spring wheat (Triticum aestivum L.) cultivar ‘Onfarom 9,’ a pot experiment was conducted using saline soil with electrical conductivity of soil paste extract (ECe) of 13.2 dS m^?1. A factorial experiment with a completely randomized design replicated seven times was used with three levels of saline irrigation water (4, 9, and 12 dS m^?1) and four leaching levels (0, 17, 29, and 37%) included as the factors. The results showed that ETc significantly decreased as a result of an increase in irrigation water salinity (ECi) and decrease in leaching level. Crop evapotranspiration deficit and decreasing irrigation and drainage water effectively resulted in grain and straw yield reduction. Increase in ECi increased accumulation of Cl and Na in crop shoot, but application of leaching decreased this accumulation.     

Functional traits associated with nitrogen use efficiency in wheat

The association between functional traits and nitrogen use efficiency (NUE) was investigated to assist the breeding of nitrogen (N) use-efficient bread wheat (Triticum aestivum ssp. aestivum) varieties. This study combined results from a climate chamber experiment involving 41 spring wheat varieties and a field experiment involving six winter and six spring wheat varieties grown with and without the application of mineral N fertiliser. The climate chamber experiment was analysed by partial least squares (PLS) regression, with several predictors and NUE as response, to identify traits related to NUE. Specific hypotheses were then tested in the field experiment. The PLS indicated six traits of particular importance for overall NUE: leaf chlorophyll (SPAD value) of the top leaf at stem elongation, grains ear^?1, ears pot^?1, straw biomass pot^?1, days between emergence and anthesis, and days between emergence and completed senescence. In the field experiment, the SPAD value of flag leaves of winter wheat around anthesis was positively correlated with NUE and total grain N, at both N levels. Fast development was positively correlated with high NUE and N uptake efficiency in spring wheat. Early senescence of the flag leaf was positively correlated with grain N concentration and negatively correlated with grain-specific N efficiency in winter wheat at low N fertilisation levels. The results indicate that high SPAD value of the top leaf might be a candidate trait that could be used in wheat breeding for improved NUE, while genetic variation in senescence could possibly be used to tailor varieties for different end-use quality when grown at low N. More studies are needed to validate these findings in other environments and for other genotypes.     

Compost and Nitrogen Management Influence Productivity of Spring Maize (Zea mays L.) under Deep and Conventional Tillage Systems in Semi-arid Regions

On-farm research was conducted to investigate the effects of nitrogen (N) and compost (C) on yield and yield components of spring maize (Zea mays L.) under conventional and deep tillage system (T) at the research farm of the University of Agriculture, Peshawar, Pakistan, during spring 2013. The experiment was laid out in a randomized complete block design with split-plot arrangement, using three replications. Three compost levels (0, 1, and 2 t ha^?1) and two tillage systems (conventional and deep tillage) were allotted to the main plot, whereas N levels (60, 90, 120, and 150 kg N ha^?1) were allotted to subplots in the form of urea. Nitrogen and compost levels had significantly affected all the parameters. Plots treated with 150 kg N ha^?1 increased ear length (31 cm), grains ear^?1 (413), thousand-grain weight (240.2 g), grain yield (3097 kg ha^?1), straw yield (9294 kg ha^?1), harvest index (24.7 percent), and shelling percentage (81.7 percent). Compost applied at 2 t ha^?1 increased ear length (32 cm), grains ear^?1 (430), thousand-grain weight (242.3 g), grain yield (2974 kg ha^?1), straw yield (8984 kg ha^?1), harvest index (24.6 percent), and shelling percentage (83.2 percent). Tillage system had significant effect on all parameters except ear length and harvest index. Deep tillage system produced more grains ear^?1 (365), thousand-grain weight (233.3 g), grain yield (2630 kg ha^?1), straw yield (8549 kg ha^?1), and shelling percentage (79.6 percent). It was concluded from the results that application of 120 kg N ha^?1 + 2 C t ha^?1 under a deep tillage system could improve spring maize yield and yield-contributing traits under semi-arid conditions.