青蒿散抗鸡球虫药效研究

为探讨青蒿散对鸡柔嫩艾美耳球虫(E.tenella)的防治效果,本试验选取90只健康黄羽肉鸡,分为6组,除空白对照组外,其余各组(感染对照组、药物对照组及青蒿散低、中、高剂量组)试验鸡均经口感染6×104个柔嫩艾美耳球虫孢子化卵囊,各试验组在接种前一天开始用药至试验结束。记录各组试验鸡的眼观病变、组织病理学检查、相对增重率、盲肠病变记分、血便记分、每克粪便中的卵囊数(OPG)、卵囊值及抗球虫指数(ACI)指标,评价青蒿散不同给药浓度的抗球虫效果。结果显示,空白对照组、感染对照组、药物对照组及青蒿散低、中、高剂量组的抗球虫指数分别为200.00、72.23、157.19、82.89、125.32和137.06,与感染对照组相比,药物对照组和青蒿散各剂量组的眼观病变、组织病理学检查均有所缓解,青蒿散能减轻病鸡盲肠的肿胀和出血等症状,且病鸡精神状态较感染对照组好;与感染对照组相比,各给药组的平均增重均有所升高,盲肠病变、卵囊值及血便情况减少。综上所述,青蒿散能缓解柔嫩艾美耳球虫侵染鸡盲肠所致的病理症状和组织病变,减少粪便中卵囊的排出数量,有一定的抗球虫效果,且给药浓度越高抗球虫效果越好,具有剂量依赖性。     

杀球灵对柔嫩艾美耳球虫作用的研究

本文报道了杀球灵(Diclazuril)对鸡柔嫩艾美耳球虫E.tenella的杀灭作用、作用峰期及停药后作用持续时间。以1ppm的推荐剂量混于饲料,分别于感染前2天、感染当天、感染后1天开始饲喂,对经口一次人工感染8×10~4/只柔嫩艾美耳球虫卵囊的鸡有显著的保护作用。试验鸡不出现血便、盲肠组织学检查及粘膜刮取物镜检均不见合子及卵囊,感染后2天给药则其抗球虫作用显著降低。故可认为杀球灵作用峰期是子孢子和第一代裂殖体阶段。停药后其抗球虫作用很快减弱或消失。     

复方海南霉素的药效试验

从某鸡场发生球虫病鸡的粪便中分离出柔嫩艾美耳球虫卵囊，经鸡体繁殖、复壮，待球虫孢子化后，配成100000个／mL的球虫卵囊液，各取一mL分别感染随机分为9组的16日龄健康岭南黄鸡苗，通过饲喂配有不同剂量抗球虫药。结果表明，复方海南霉素能提高抗球虫效果。     

纯中草药散剂对鸡球虫病的疗效试验

250只海蓝灰雏鸡，饲养至15日龄，随机分为5组，分别为试验组（1％纯中药散剂组、0.5％纯中药散剂组、0.25％纯中药散剂组）、阳性对照组（只感染球虫卵囊不给药）及药物（三字球虫粉）对照组，每组50只。试验鸡全部经口感染鸡柔嫩艾美耳球虫孢子化卵囊，试验组及药物对照组连续给药5 d。在第8 d将鸡全部扑杀剖检。试验期间统计每组鸡的死亡率、增重率、粪便记分、病变记分、病变值、卵囊计数、卵囊比数、卵囊值、鸡抗球虫指数等指标。结果表明：0.5％～1％的纯中药散剂抗球虫指数在160～180之间，为中效抗球虫药。     

不同发育期两种艾美耳球虫在鸡消化道内的分布

对柔嫩艾美耳球虫Eimeria teenlla和巨型艾美耳球虫Eimeria maxima的卵囊、孢子囊和子孢子在鸡消化道和粪便中的分布情况进行了研究。口服接种柔嫩艾美耳球虫卵囊第1小时后,盲肠内孢子囊数为3.4×10~8、以后则逐渐减少;而子孢子数增加且直到接种后第12小时仍保持一个高水平。其它肠段只有少量的孢子囊和子孢子。在接种巨型艾美耳球虫卵囊后的第2小时,主要在空肠内发现有大量的子孢子。研究发现,柔嫩艾美耳球虫和巨型艾美耳球虫的绝大多数子孢子分别寄居在盲肠和空肠内,表明每种球虫子孢子侵袭部位的特异性在侵袭发生前就确定了。     

青蒿散抗鸡球虫药效研究

为探讨青蒿散对鸡柔嫩艾美耳球虫（E.tenella）的防治效果,本试验选取90只健康黄羽肉鸡,分为6组,除空白对照组外,其余各组（感染对照组、药物对照组及青蒿散低、中、高剂量组）试验鸡均经口感染6×10⁴个柔嫩艾美耳球虫孢子化卵囊,各试验组在接种前一天开始用药至试验结束。记录各组试验鸡的眼观病变、组织病理学检查、相对增重率、盲肠病变记分、血便记分、每克粪便中的卵囊数（OPG）、卵囊值及抗球虫指数（ACI）指标,评价青蒿散不同给药浓度的抗球虫效果。结果显示,空白对照组、感染对照组、药物对照组及青蒿散低、中、高剂量组的抗球虫指数分别为200.00、72.23、157.19、82.89、125.32和137.06,与感染对照组相比,药物对照组和青蒿散各剂量组的眼观病变、组织病理学检查均有所缓解,青蒿散能减轻病鸡盲肠的肿胀和出血等症状,且病鸡精神状态较感染对照组好;与感染对照组相比,各给药组的平均增重均有所升高,盲肠病变、卵囊值及血便情况减少。综上所述,青蒿散能缓解柔嫩艾美耳球虫侵染鸡盲肠所致的病理症状和组织病变,减少粪便中卵囊的排出数量,有一定的抗球虫效果,且给药浓度越高抗球虫效果越好,具有剂量依赖性。     

张家口市鸡柔嫩艾美耳球虫对磺胺氯吡嗪钠的耐药性

为了解张家口市鸡柔嫩艾美耳球虫耐磺胺氯吡嗪钠的情况，试验选取张家口市坝下地区蔚县、宣化区和坝上地区张北县、沽源县以磺胺氯吡嗪钠作为抗球虫药的养鸡场进行球虫耐药性检测。从采集的粪便样本中进行卵囊分离，宣化区和张北县粪便样本中检出球虫卵囊，蔚县和沽源县未检出卵囊。将检出卵囊的粪便样本进行卵囊分离保存，经耐药性试验发现，宣化地区柔嫩艾美耳球虫株对磺胺氯吡嗪钠的敏感浓度为9.6mg/kg，张北县为4.8mg/kg。本次试验结果可为张家口市养鸡场防治鸡柔嫩艾美耳球虫的给药浓度提供一定数据支持，有效缓解因球虫耐药性对养鸡产业造成的危害及经济损失。     

白头翁不同提取物及复方抗鸡柔嫩艾美耳球虫研究

以存活率、相对增重率、盲肠病变记分、卵囊值和抗球虫指数为判定指标,考察白头翁不同提取物及复方对鸡柔嫩艾美耳球虫孢子化卵囊致病性的影响(体外试验)和抗鸡柔嫩艾美耳球虫感染效果(体内试验)。体内、体外试验结果均表明,白头翁不同提取物及复方均能提高相对增重率,降低盲肠病变计分和卵囊值。抗球虫指数(ACI)检测表明,白头翁不同提取物及复方对球虫孢子化卵囊有微弱的抑杀效果,对鸡柔嫩艾美耳球虫感染预防效果较差,不宜作为鸡柔嫩艾美耳球虫病的预防和治疗药物。     

海南霉素对鸡球虫病的预防和治疗效果评价

为评价海南霉素对鸡球虫病的防治效果 ,作者用海南霉素和马杜拉霉素分别进行了抗鸡球虫病的预防和治疗试验。二个试验各设 7个组 ,每组 12羽 17日龄 AA肉鸡 ;在 18日龄 ,除不感染组外 ,每羽鸡接种柔嫩艾美耳球虫孢子化卵囊 6万 ;药物组分别在感染前 1天和感染后第 4天 ,开始投服含药饲料 ;感染后第 8天结束试验 ,以抗球虫指数、病变记分、卵囊数、粪便记分等作为试验指标。结果在预防试验中 ,海南霉素的平均抗球虫指数为 182 ,病变记分减少 96 % ,粪便和盲肠卵囊数减少 98% ,粪便记分为 0 ;在治疗试验中 ,海南霉素的平均抗球虫指数为 14 6 ,病变记分减少 33% ,粪便和盲肠卵囊数减少 5 3%。海南霉素是一种高效抗鸡球虫病药物 ,其预防效果明显优于治疗效果     

中药复方制剂对球虫感染鸡血液免疫指标和酶活性影响

为了研究中草药复方制剂对人工感染柔嫩艾美耳球虫的鸡血液免疫指标和酶活性影响,选取80只罗曼蛋公雏饲喂至24日龄,随机分为4组,分别为非感染给药组、非感染非给药组、感染非给药组和感染给药组。27日龄时,感染组每只鸡接种1×105个孢子化球虫卵囊。感染球虫8d后,心脏采血,收集血清,采用半自动生化分析仪测定血清免疫指标:总蛋白(TP),白蛋白(ALB),球蛋白,免疫球蛋白IgA、IgG、IgM,血清酶指标:谷丙转氨酶(GPT)、谷草转氨酶(GOT)、碱性磷酸酶(ALP)。结果表明非感染非给药组的总蛋白、白蛋白、球蛋白血清浓度明显高于感染组,而感染给药组与非感染非给药组之间差异不显著(P0.05);感染组IgA、IgM含量显著高于非感染非给药组(P0.01),而IgG含量则显著低于非感染非给药组(P0.01),IgA、IgM含量感染给药组显著低于感染非给药组(P0.01),IgG含量感染给药组显著高于感染非给药组(P0.01);与非感染非给药组相比,感染组3种血清酶的活性均明显下降,感染给药组各种酶的活性均显著高于感染非给药组(P0.01)。表明该中草药复方制剂可降低柔嫩艾美耳球虫对鸡血清蛋白浓度的影响,使血清酶活性升高。     

Avian eimeria: invasion in foreign host birds and generation of partial immunity against coccidiosis

Four species of avian Eimeria invaded the intestine of foreign host birds in the same areas in which they invaded the natural host. Repeated inoculation (immunization) of chickens with the turkey coccidian, Eimeria adenoeides, partially protected the chickens against a subsequent challenge with 5.8 x 10(4) E. tenella oocysts. At 6 days post-challenge, the weight gain and feed conversion efficiency of the immunized chickens was significantly better than those of the chickens that were not immunized with E. adenoeides. Lesion scores and cellular invasion by the sporozoites were significantly lower in the immunized birds than in the unimmunized group. Electrophoresis and Western blot analysis identified changes in the serum antibody profiles of the chickens that appeared to be associated with the immunization and challenge programs. An antibody or antibodies recognizing a 60,000-molecular-weight antigen of E. tenella sporozoites disappeared when chickens immunized with E. adenoeides were challenged with E. tenella; an antibody or antibodies recognizing a 23,000-molecular-weight sporozoite antigen appeared within 6 days of challenge. Reciprocal studies, in which turkeys were immunized with E. tenella and challenged with E. adenoeides, showed little evidence of protection.     

硫酸化酵母葡聚糖对鸡球虫病的防治效果

旨在探讨硫酸化酵母葡聚糖（sGSC）对人工感染鸡柔嫩艾美耳球虫（Eimeria tenella）病的防治效果。采用笼饲法，将200只鸡随机分为A组（4 mg·kg^-1）、B组（16 mg·kg^-1）、C组（64 mg·kg^-1）、D组（感染对照组）和E组（健康对照组）。感染球虫前3 d开始，给A、B、C组鸡每天按体重饮水喂上述各浓度的sGSC，给D、E组的鸡喂纯水（RO水），持续至试验结束。试验过程中观察各组鸡的临床症状、血便情况并记录，收集感染后5～6 d的粪便，检测每克粪便中的卵囊数，感染后第7天剖杀所有鸡，计算脏器指数；并通过Illumina测序平台研究鸡盲肠内微生物群落多样性。结果显示：1）与D组比较，各给糖组鸡的盲肠肿胀明显减轻，肠内容物和出血明显减少，与E组鸡体重相比，A、D组体重明显降低（P<0.05），但B组和C组鸡的体重升高至接近E组水平，体重与E组鸡无显著差异（P>0.05）；B组和C组鸡排出的卵囊数与D组相比，出现了明显的减少，但无统计学差异（P>0.05）；A组鸡的胸腺指数明显增加（P<0.05），肝指数也出现显著改善（P<0.05）；2）对盲肠内菌群分析发现，sGSC能提高盲肠内韦荣氏菌科（Veillonellaceae）和乳酸杆菌科（Lactobacillaceae）的相对丰度，降低肠球菌科（Enterococcaceae）的相对丰度。sGSC能减轻柔嫩艾美尔球虫感染对鸡造成的危害，且随着sGSC剂量的增加，效果越明显。综上表明，sGSC具有一定的抗球虫效果，并且对感染球虫鸡肠道内的菌群具有有益的调节作用。     

石榴皮提取物对离体培养兔球虫卵囊的杀灭作用

本试验旨在研究石榴皮提取物对离体培养兔球虫卵囊的杀灭作用。采集已感染球虫的18只家兔(体重2.0~2.5 kg)的新鲜粪便(24 h内,35 g/只),用饱和盐水漂浮法分离、提取球虫卵囊。球虫卵囊分别在蒸馏水、石榴皮水提物、石榴皮黄酮、石榴皮多酚、盐酸氯苯胍中培养24、48、72 h,光镜下观察不同浓度的各种药物对兔球虫卵囊数量的影响。结果显示,与蒸馏水组比较,5、10 mg/mL剂量组培养72 h,4种药物均使兔球虫卵囊数量明显减少。球虫卵囊的减少率石榴皮水提物组大于石榴皮黄酮组,石榴皮黄酮组大于石榴皮多酚组和盐酸氯苯胍组;起效时间约为48~72 h。1 mg/mL剂量组4种药物虽可使兔球虫卵囊数量减少,但无显著差异(P＞0.05)。结果提示,石榴皮提取物对体外培养的兔球虫卵囊有较强的杀灭作用,其中石榴皮水提物对兔球虫卵囊杀灭作用最强,石榴皮黄酮和石榴皮多酚次之,其杀灭作用呈一定的正相关量效关系。     

Effect of resinous extract from Commiphora swynnertonii (Burrt) on experimental coccidial infection in chickens

A crude resinous extract from Commiphora swynnertonii was tested against an experimental coccidial infection in local chickens. A total of 80 growing chickens were randomly assigned into five groups, which received different treatments. Chickens in G1 were not infected with coccidian oocysts and therefore served as a negative control. All chickens in G2, G3, G4 and G5 were infected through oral administration of coccidian oocysts suspension at a dosed rate of 1.5?×?10⁴ Eimeria spp. oocysts per bird. Starting from day 3 post-infection (p.i), chickens in different groups were treated for 7 consecutive days as follows: G1 and G2 (positive control) received 5 ml of normal saline as placebo, G3 and G4 were given the extract at 400 and 800 mg/kg bodyweight whereas G5 received anticoccidial drug. Clinical signs, bodyweights, oocysts counts and mortality rates were observed regularly. Results showed that oral administration of the resinous extract to chickens with coccidiosis significantly reduced mortality rate from 94 to 25 % and oocysts counts from 1.03?×?10⁵ to 6.55?×?10³ oocysts/g faeces (p?<?0.05). Also a body condition score chart indicated less severe clinical signs of the disease in the groups which received the extract. Mean daily body weights were slightly reduced by the administration of the extract but this effect disappeared by day 7 p.i. These findings clearly indicate that resinous extract from C. swynnertonii has significant anticoccidial effect against experimental Eimeria spp. infection in chickens. A larger field trial to validate the use of the extract in chickens naturally infected with Eimeria spp. is required.     

假蒟提取物的体外抗氧化和抗炎效果研究

本研究旨在分析假蒟提取物的活性成分并评价其体外抗氧化和抗炎活性。试验通过ABTS法检测3种假蒟提取物(石油醚、正丁醇及乙酸乙酯提取物)的抗氧化能力;采用LPS法建立猪小肠上皮细胞(IPEC-J2)体外炎症模型,用假蒟提取物预处理后,以ELISA法检测细胞上清液中肿瘤坏死因子(TNF-α)、白细胞介素-1(IL-1)和白细胞介素-6(IL-6)的含量。结果表明,3种假蒟提取物总抗氧化活性由大到小依次是假蒟正丁醇提取物、乙酸乙酯提取物、石油醚提取物(P<0.05),其中假蒟正丁醇提取物总生物碱、总酚含量最高,分别为2.81 mEq/100 g、161.82 mg/g。与空白组相比,LPS组IPEC-J2细胞的TNF-α(P<0.05)、IL-1(P>0.05)及IL-6(P<0.05)含量均升高;与LPS组相比,假蒟预处理组的这几种细胞因子含量均显著降低(P<0.05),由高到低依次为乙酸乙酯提取物>石油醚提取物>正丁醇提取物。综合试验结果,假蒟提取物具有一定的抗氧化作用,能影响IPEC-J2细胞炎性因子的分泌,抑制炎症反应。     

Experimental infection of budgerigars (Melopsittacus undulatus) with a low virulent K21 strain of Toxoplasma gondii

In total 53 budgerigars (Melopsittacus undulatus) were divided into six groups and orally infected with a suspension of oocysts of low virulent Toxoplasma gondii K21 strain in the doses of 10(2), 10(3), 10(4), 10(5) and 10(6), respectively. Blood was collected from the birds prior to the inoculation and then on days 10, 20 and 30 post infection. Latex-agglutination test (LAT) was used for the detection of antibodies in the inoculated birds. The infected birds showed no apparent signs of disease. The antibodies were found in all but two birds inoculated a dose of 10(2) oocysts. Haematological values remained unchanged after infection. T. gondii was isolated by bioassay in mice from all 37 birds fed 10(3) or more oocysts and 6 of 9 fed 10(2) oocysts. The results demonstrate that budgerigars are resistant to T. gondii infection.     

检测鸡球虫四价弱毒疫苗抗体应答的ELISA方法的建立及初步应用

本研究旨在建立检测鸡球虫四价弱毒疫苗(柔嫩艾美耳球虫、毒害艾美耳球虫、堆型艾美耳球虫、巨型艾美耳球虫)抗体应答的酶联免疫吸附试验(ELISA),并初步阐明鸡免疫四价弱毒疫苗及攻虫后的抗体应答。将300羽岭南黄鸡分为A(免疫攻虫组)、B(不免疫不攻虫组)、C(不免疫攻虫组)共3个组。A组雏鸡在4日龄时进行首免,11日龄时鸡开始二免,二免完成后(17日龄时)对A组及C组的鸡进行攻虫(28万个4种球虫的混合卵囊/羽),7 d后检查A、C两组鸡的存活率。在鸡二免和三免完成后,分别对A、B组进行采血,通过优化最佳抗原包被浓度、酶结合物工作浓度及最佳血清稀释度,建立了ELISA方法来检测鸡体对球虫四价弱毒疫苗的抗体应答情况。A组鸡血清抗体的平均D值为0.870和0.904,明显高于B组鸡的平均D值0.261和0.270,证明了鸡体免疫疫苗后可以刺激产生相应抗体。免疫鸡攻虫后的存活率和抗体应答的检测结果,都充分说明了此鸡球虫弱毒四价疫苗具有良好的免疫原性,所建立的ELISA方法为今后评价鸡球虫弱毒四价疫苗的免疫效力提供了有效手段。     

槟榔提取物对球虫感染鸡血液指标及抗球虫效果的影响

本试验旨在研究槟榔提取物对感染柔嫩艾美耳球虫鸡血液指标及抗球虫效果的影响。选取270只1日龄文昌鸡公鸡,随机分为6个组,分别为空白对照组、阴性对照组、阳性对照组及槟榔1、2、3组,其中,3个对照组均饲喂雏鸡基础日粮,槟榔1、2、3组分别饲喂在基础日粮中添加200、400、600 mg/kg槟榔提取物粉剂的试验日粮。15日龄时,随机从每组中抽取30只体重相近的鸡,除空白对照组外,各组每只试验鸡经口灌服1 mL含1×10⁵个球虫孢子化卵囊的生理盐水混合液。结果表明:①感染后第6、9天,与空白对照组、阳性对照组相比,槟榔1、2、3组和阴性对照组血液中红细胞数、血红蛋白浓度、红细胞比容降低;②感染后第3、9天,与空白对照组相比,阳性对照组和槟榔1组血清中总蛋白、白蛋白、球蛋白含量升高;感染后第9天,槟榔2、3组血清中谷丙转氨酶含量较空白对照组、阳性对照组显著降低(P<0.05),槟榔1组血清中尿酸含量较阴性对照组、阳性对照组及槟榔2、3组显著降低(P<0.05);感染后第6天,阴性对照组血清中Na⁺、Cl^-浓度较空白对照组、阳性对照组显著降低(P<0.05),槟榔1组Na⁺、Cl^-浓度与3个对照组间差异均不显著(P>0.05);③槟榔1、2、3组的抗球虫指数分别为128.48、126.53和141.11。综上所述,日粮中添加200、400和600 mg/kg槟榔提取物粉剂对于球虫感染鸡抗球虫药效中等;200、400 mg/kg槟榔提取物粉剂可维持感染球虫鸡血清中Na⁺、Cl^-浓度的稳定,降低肝脏损伤。     

Effects of dietary tannic acid and vaccination on the course of coccidiosis in experimentally challenged broiler chicken

An experiment was carried out to assess the influence of tannic acid (TA) on integrity of the intestine in broiler chicks vaccinated against coccidiosis and challenged with the disease. In a 2 × 2 factorial design, the trial had five groups of 10 chickens each, including positive (group 2) and negative (group 1) controls. The chickens were kept on wood shavings and fed a commercial maize and soybean-based starter-grower diet. From day 1, groups 3 and 5 received TA (10 g kg(-1)) in their diet. On day 4, birds of groups 4 and 5 were vaccinated orally against coccidiosis with anti-coccidial vaccine, Livacox T?. Each dose of the vaccine contained 300-500 sporulated oocysts of each of Eimeria acervulina, Eimeria maxima and Eimeria tenella. On day 18, all experimental groups except for the negative (group 1) were challenged with 10-fold dose of Livacox T? to produce a mild coccidiosis infection. Faecal samples of individual birds were collected on day 23, and the number of faecal oocysts was determined. d-Xylose absorption test was also carried out on all birds on day 23. Immediately after d-xylose absorption test, all birds were killed humanely and the intestinal tract was removed, weighed and examined for gross lesions. Results showed that negative (group 1) and positive controls (group 2) had the highest and lowest levels of plasma d-xylose post-ingestion of the substrate, respectively. Vaccination and/or feeding TA raised the level of plasma d-xylose in infected birds, although this was not significant for TA-fed birds. Vaccination reduced but TA increased the total number of oocysts per gram of faeces. Birds of groups 2-5 had distinct intestinal lesions when compared with group 1. However, vaccination prevented intestinal lesions. Relative weights of intestinal parts were the lowest in group 1 and the highest in group 2. Vaccination but not TA reduced the relative weights of intestinal parts in infected birds. It was concluded that dietary tannins may reduce the efficacy of anticoccidial vaccines and alter the proper development of immunity against the disease.     

柔嫩艾美耳球虫对藏鸡和隐性白羽鸡致病性差异分析

为进一步明确柔嫩艾美耳球虫对球虫易感性差异鸡种的致病性,本研究以1×10⁵个柔嫩艾美耳球虫孢子化卵囊的剂量分别感染对球虫具有抗性的藏鸡和易感的隐性白羽鸡,接种后观察记录各组鸡的临床表征、血便记分、死亡率、增重、盲肠病变记分、卵囊产量,并于感染前和感染后3、6和8 d每个品种分别随机选择5只鸡采集抗凝血,应用流式细胞仪（FCAS）检测外周血CD4⁺T和CD8⁺T淋巴细胞亚群数量。结果显示,柔嫩艾美耳球虫感染后藏鸡相对增重率高于隐性白羽鸡,死亡率、血便记分和盲肠病变记分均低于隐性白羽鸡,但卵囊产量高于隐性白羽鸡。外周血T淋巴细胞亚群变化方面,感染前,藏鸡CD4⁺T淋巴细胞数及CD4⁺/CD8⁺比值均高于隐性白羽鸡。感染后第3天,藏鸡CD4⁺、CD8⁺ T淋巴细胞数及CD4⁺/CD8⁺比值下降,隐性白羽鸡CD8⁺ T淋巴细胞数略有下降,CD4⁺T淋巴细胞数及CD4⁺/CD8⁺比值上升,但CD4⁺/CD8⁺比值仍显著低于藏鸡（P<0.05）。感染后第6天,2个品种鸡CD4⁺ T淋巴细胞数及CD4⁺/CD8⁺比值均下降,其中藏鸡表现为显著下降（P<0.05）,而隐性白羽鸡仅CD4⁺/CD8⁺比值显著降低（P<0.05）,隐性白羽鸡CD8⁺ T淋巴细胞数显著升高（P<0.05）。感染后第8天,2个品种鸡CD4⁺/CD8⁺比值显著下降（P<0.05）,藏鸡CD8⁺ T淋巴细胞数显著高于隐性白羽鸡（P<0.05）,但CD4⁺/CD8⁺比值显著低于隐性白羽鸡（P<0.05）。结果表明,球虫对藏鸡和隐性白羽鸡的致病性存在差异,这种差异与T淋巴细胞介导的免疫应答反应密切相关。